The Experts below are selected from a list of 321 Experts worldwide ranked by ideXlab platform
Philippe Rott - One of the best experts on this subject based on the ideXlab platform.
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towards identification of mutations associated with variation in Pathogenicity among nine genetically closely related strains of xanthomonas albilineans from guadeloupe poster 38
11èmes Rencontres Plantes-Bactéries Aussois France 3-7 février 2014, 2014Co-Authors: Isabelle Pieretti, Philippe Rott, Stephane Cociancich, Stephanie Bolot, Sebastien Carrere, Pierre Lefeuvre, Monique RoyerAbstract:Xanthomonas albilineans causes leaf scald, a lethal disease of sugarcane. This bacterium exhibits distinctive pathogenic mechanisms, ecology and taxonomy when compared to other species of Xanthomonas. For example, X. albilineans is missing two loci required for Pathogenicity in other plant ·pathogenic species of Xanlhol11onas: the xanthan gum biosynthesis and the Hrp (for Hypersensitive response and pathogenicily) TTss (for Type ThreeSecretion System) gene clusters. X albilineans also exhibits a large intra-species variability which was previously described using different genetic markers, including PFGE (for PulsedField Gel Electrophoresis) ( 1). Aerial transmission of the pathogen has been shown to occur in several sugarcane producing locations of the world (including Florida, Guadeloupe, Louisiana, Mauritius, Taiwan, and Texas), and was associated with outbreaks of the disease in the late 1980's. Strain s of X albilineans involved in these out breaks were shown to belong to a single PFGE group called PFGE-B (1). A former study revealed high variation in Pathogenicity among 19 PFGE-B strains isolated in Guadeloupe in 2003 (2). These 19 strains, which differ in their capacity to colonize sugarcane stalks and/or to cause foliar symptoms, were distributed into 4 colonization groups (CG): CG-I to CG-IV with CG-I including the least aggressive strains and CG-IV the most aggressive strains. AFLP analyses were performed using 9 of these 19 strains which span the variability o f Pathogenicity (CG- I la CG-IV), and results revealed that these 9 strain s were genetically c10sely related. However, no relationship was found between the AFLP genetic variation and the variation in pathogcnicity (2). In the present stud y, we sequenced these nine strains in order to identify SNP (for Single Nucleotide Polymorphism) associated with the distribution of the strains into the 4 groups of colonization. (Resume d' auteur)
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Identification of new candidate Pathogenicity factors in the xylem-invading pathogen Xanthomonas albilineans by transposon mutagenesis
Molecular Plant-microbe Interactions, 2011Co-Authors: Philippe Rott, Monique Royer, Gary C Marlow, Laura Fleites, Dean W GabrielAbstract:Xanthomonas albilineans is a xylem-invading pathogen that produces the toxin albicidin that blocks chloroplast differentiation, resulting in disease symptoms of sugarcane leaf scald. In contrast to other xanthomonads, X. albilineans does not possess a hypersensitive response and Pathogenicity type III secretion system and does not produce xanthan gum. Albicidin is the only previously known Pathogenicity factor in X. albilineans, yet albicidin-deficient mutant strains are still able to efficiently colonize sugarcane. To identify additional host adaptation or Pathogenicity factors, sugarcane ‘CP80-1743’ was inoculated with 1,216 independently derived Tn5 insertions in X. albilineans XaFL07-1 from Florida. Sixty-one Tn5 mutants were affected in development of leaf symptoms or in stalk colonization. The Tn5 insertion sites of these mutants were determined and the interrupted genes were identified using the recently available genomic DNA sequence of X. albilineans GPE PC73 from Guadeloupe. Several pathogenicit...
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high variation in Pathogenicity of genetically closely related strains of xanthomonas albilineans the sugarcane leaf scald pathogen in guadeloupe
Phytopathology, 2006Co-Authors: Patrice Champoiseau, Isabelle Pieretti, Monique Royer, Stephane Cociancich, Jean-heinrich Daugrois, Philippe RottAbstract:Pathogenicity of 75 strains of Xanthomonas albilineans from Guadeloupe was assessed by inoculation of sugarcane cv. B69566, which is susceptible to leaf scald, and 19 of the strains were selected as representative of the variation in Pathogenicity observed based on stalk colonization. In vitro production of albicidin varied among these 19 strains, but the restriction fragment length polymorphism pattern of their albicidin biosynthesis genes was identical. Similarly, no genomic variation was found among strains by pulsed-field gel electrophoresis. Some variation among strains was found by amplified fragment length polymorphism, but no relationship between this genetic variation and variation in Pathogenicity was found. Only 3 (pilB, rpfA, and xpsE) of 40 genes involved in Pathogenicity of bacterial species closely related to X. albilineans could be amplified by polymerase chain reaction from total genomic DNA of all nine strains tested of X. albilineans differing in Pathogenicity in Guadeloupe. Nucleotide sequences of these genes were 100% identical among strains, and a phylogenetic study with these genes and housekeeping genes efp and ihfA suggested that X. albilineans is on an evolutionary road between the X. campestris group and Xylella fastidiosa, another vascular plant pathogen. Sequencing of the complete genome of Xanthomonas albilineans could be the next step in deciphering molecular mechanisms involved in Pathogenicity of X. albilineans.
Dean W Gabriel - One of the best experts on this subject based on the ideXlab platform.
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Identification of new candidate Pathogenicity factors in the xylem-invading pathogen Xanthomonas albilineans by transposon mutagenesis
Molecular Plant-microbe Interactions, 2011Co-Authors: Philippe Rott, Monique Royer, Gary C Marlow, Laura Fleites, Dean W GabrielAbstract:Xanthomonas albilineans is a xylem-invading pathogen that produces the toxin albicidin that blocks chloroplast differentiation, resulting in disease symptoms of sugarcane leaf scald. In contrast to other xanthomonads, X. albilineans does not possess a hypersensitive response and Pathogenicity type III secretion system and does not produce xanthan gum. Albicidin is the only previously known Pathogenicity factor in X. albilineans, yet albicidin-deficient mutant strains are still able to efficiently colonize sugarcane. To identify additional host adaptation or Pathogenicity factors, sugarcane ‘CP80-1743’ was inoculated with 1,216 independently derived Tn5 insertions in X. albilineans XaFL07-1 from Florida. Sixty-one Tn5 mutants were affected in development of leaf symptoms or in stalk colonization. The Tn5 insertion sites of these mutants were determined and the interrupted genes were identified using the recently available genomic DNA sequence of X. albilineans GPE PC73 from Guadeloupe. Several pathogenicit...
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Intragenic recombination of a single plant pathogen gene provides a mechanism for the evolution of new host specificities.
Journal of bacteriology, 1995Co-Authors: Yinong Yang, Dean W GabrielAbstract:Gene pthA is required for virulence of Xanthomonas citri on citrus plants and has pleiotropic Pathogenicity and avirulence functions when transferred to many different xanthomonads. DNA sequencing revealed that pthA belongs to a family of Xanthomonas avirulence/Pathogenicity genes characterized by nearly identical 102-bp tandem repeats in the central region. By inserting an nptI-sac cartridge into the tandemly repeated region of pthA as a selective marker, intragenic recombination among homologous repeats was observed in both Xanthomonas spp. and Escherichia coli. Intragenic recombination within pthA created new genes with novel host specificities and altered Pathogenicity and/or avirulence phenotypes. Many pthA recombinants gained or lost avirulence function in Pathogenicity assays on bean, citrus, and cotton cultivars. Although the ability to induce cell division (hyperplastic cankers) on citrus could be lost, this ability was not acquired on cotton or bean plants. Intragenic recombination therefore provides a genetic mechanism for the generation of multiple, different, and gratuitous avirulence genes from a single, required, host-specific Pathogenicity gene.
Monique Royer - One of the best experts on this subject based on the ideXlab platform.
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towards identification of mutations associated with variation in Pathogenicity among nine genetically closely related strains of xanthomonas albilineans from guadeloupe poster 38
11èmes Rencontres Plantes-Bactéries Aussois France 3-7 février 2014, 2014Co-Authors: Isabelle Pieretti, Philippe Rott, Stephane Cociancich, Stephanie Bolot, Sebastien Carrere, Pierre Lefeuvre, Monique RoyerAbstract:Xanthomonas albilineans causes leaf scald, a lethal disease of sugarcane. This bacterium exhibits distinctive pathogenic mechanisms, ecology and taxonomy when compared to other species of Xanthomonas. For example, X. albilineans is missing two loci required for Pathogenicity in other plant ·pathogenic species of Xanlhol11onas: the xanthan gum biosynthesis and the Hrp (for Hypersensitive response and pathogenicily) TTss (for Type ThreeSecretion System) gene clusters. X albilineans also exhibits a large intra-species variability which was previously described using different genetic markers, including PFGE (for PulsedField Gel Electrophoresis) ( 1). Aerial transmission of the pathogen has been shown to occur in several sugarcane producing locations of the world (including Florida, Guadeloupe, Louisiana, Mauritius, Taiwan, and Texas), and was associated with outbreaks of the disease in the late 1980's. Strain s of X albilineans involved in these out breaks were shown to belong to a single PFGE group called PFGE-B (1). A former study revealed high variation in Pathogenicity among 19 PFGE-B strains isolated in Guadeloupe in 2003 (2). These 19 strains, which differ in their capacity to colonize sugarcane stalks and/or to cause foliar symptoms, were distributed into 4 colonization groups (CG): CG-I to CG-IV with CG-I including the least aggressive strains and CG-IV the most aggressive strains. AFLP analyses were performed using 9 of these 19 strains which span the variability o f Pathogenicity (CG- I la CG-IV), and results revealed that these 9 strain s were genetically c10sely related. However, no relationship was found between the AFLP genetic variation and the variation in pathogcnicity (2). In the present stud y, we sequenced these nine strains in order to identify SNP (for Single Nucleotide Polymorphism) associated with the distribution of the strains into the 4 groups of colonization. (Resume d' auteur)
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Identification of new candidate Pathogenicity factors in the xylem-invading pathogen Xanthomonas albilineans by transposon mutagenesis
Molecular Plant-microbe Interactions, 2011Co-Authors: Philippe Rott, Monique Royer, Gary C Marlow, Laura Fleites, Dean W GabrielAbstract:Xanthomonas albilineans is a xylem-invading pathogen that produces the toxin albicidin that blocks chloroplast differentiation, resulting in disease symptoms of sugarcane leaf scald. In contrast to other xanthomonads, X. albilineans does not possess a hypersensitive response and Pathogenicity type III secretion system and does not produce xanthan gum. Albicidin is the only previously known Pathogenicity factor in X. albilineans, yet albicidin-deficient mutant strains are still able to efficiently colonize sugarcane. To identify additional host adaptation or Pathogenicity factors, sugarcane ‘CP80-1743’ was inoculated with 1,216 independently derived Tn5 insertions in X. albilineans XaFL07-1 from Florida. Sixty-one Tn5 mutants were affected in development of leaf symptoms or in stalk colonization. The Tn5 insertion sites of these mutants were determined and the interrupted genes were identified using the recently available genomic DNA sequence of X. albilineans GPE PC73 from Guadeloupe. Several pathogenicit...
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high variation in Pathogenicity of genetically closely related strains of xanthomonas albilineans the sugarcane leaf scald pathogen in guadeloupe
Phytopathology, 2006Co-Authors: Patrice Champoiseau, Isabelle Pieretti, Monique Royer, Stephane Cociancich, Jean-heinrich Daugrois, Philippe RottAbstract:Pathogenicity of 75 strains of Xanthomonas albilineans from Guadeloupe was assessed by inoculation of sugarcane cv. B69566, which is susceptible to leaf scald, and 19 of the strains were selected as representative of the variation in Pathogenicity observed based on stalk colonization. In vitro production of albicidin varied among these 19 strains, but the restriction fragment length polymorphism pattern of their albicidin biosynthesis genes was identical. Similarly, no genomic variation was found among strains by pulsed-field gel electrophoresis. Some variation among strains was found by amplified fragment length polymorphism, but no relationship between this genetic variation and variation in Pathogenicity was found. Only 3 (pilB, rpfA, and xpsE) of 40 genes involved in Pathogenicity of bacterial species closely related to X. albilineans could be amplified by polymerase chain reaction from total genomic DNA of all nine strains tested of X. albilineans differing in Pathogenicity in Guadeloupe. Nucleotide sequences of these genes were 100% identical among strains, and a phylogenetic study with these genes and housekeeping genes efp and ihfA suggested that X. albilineans is on an evolutionary road between the X. campestris group and Xylella fastidiosa, another vascular plant pathogen. Sequencing of the complete genome of Xanthomonas albilineans could be the next step in deciphering molecular mechanisms involved in Pathogenicity of X. albilineans.
U Desselberger - One of the best experts on this subject based on the ideXlab platform.
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Rotavirus Pathogenicity.
Virology, 1996Co-Authors: B Burke, U DesselbergerAbstract:The Pathogenicity of rotaviruses depends on multiple viral and host factors. In this review the evidence for the involvement of a number of viral genes in the ability to cause disease is presented. Different genes are of importance in different rotavirus-host systems: there is no single Pathogenicity factor.
Patrice Champoiseau - One of the best experts on this subject based on the ideXlab platform.
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high variation in Pathogenicity of genetically closely related strains of xanthomonas albilineans the sugarcane leaf scald pathogen in guadeloupe
Phytopathology, 2006Co-Authors: Patrice Champoiseau, Isabelle Pieretti, Monique Royer, Stephane Cociancich, Jean-heinrich Daugrois, Philippe RottAbstract:Pathogenicity of 75 strains of Xanthomonas albilineans from Guadeloupe was assessed by inoculation of sugarcane cv. B69566, which is susceptible to leaf scald, and 19 of the strains were selected as representative of the variation in Pathogenicity observed based on stalk colonization. In vitro production of albicidin varied among these 19 strains, but the restriction fragment length polymorphism pattern of their albicidin biosynthesis genes was identical. Similarly, no genomic variation was found among strains by pulsed-field gel electrophoresis. Some variation among strains was found by amplified fragment length polymorphism, but no relationship between this genetic variation and variation in Pathogenicity was found. Only 3 (pilB, rpfA, and xpsE) of 40 genes involved in Pathogenicity of bacterial species closely related to X. albilineans could be amplified by polymerase chain reaction from total genomic DNA of all nine strains tested of X. albilineans differing in Pathogenicity in Guadeloupe. Nucleotide sequences of these genes were 100% identical among strains, and a phylogenetic study with these genes and housekeeping genes efp and ihfA suggested that X. albilineans is on an evolutionary road between the X. campestris group and Xylella fastidiosa, another vascular plant pathogen. Sequencing of the complete genome of Xanthomonas albilineans could be the next step in deciphering molecular mechanisms involved in Pathogenicity of X. albilineans.
