The Experts below are selected from a list of 6885 Experts worldwide ranked by ideXlab platform
Thomas Kroj - One of the best experts on this subject based on the ideXlab platform.
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Optimization of pathogenicity assays to study the Arabidopsis thaliana–Xanthomonas campestris pv. campestris Pathosystem
Molecular plant pathology, 2005Co-Authors: Damien F. Meyer, Emmanuelle Lauber, Dominique Roby, Matthieu Arlat, Thomas KrojAbstract:SUMMARY The cruciferous weed Arabidopsis thaliana and the causal agent of black rot disease of Crucifers Xanthomonas campestris pv. campestris ( Xcc ) are both model organisms in plant pathology. Their interaction has been studied successfully in the past, but these investigations suffered from high variability. In the present study, we describe an improved Arabidopsis- Xcc Pathosystem that is based on a wound inoculation procedure. We show that after wound inoculation, Xcc colonizes the vascular system of Arabidopsis leaves and causes typical black rot symptoms in a compatible interaction, while in an incompatible interaction bacterial multiplication is inhibited. The highly synchronous and reproducible symptom expression allowed the development of a disease scoring scheme that enabled us to analyse the effects of mutations in individual genes on plant resistance or on bacterial virulence in a simple and precise manner. This optimized Arabidopsis- Xcc Pathosystem will be a robust tool for further genetic and post-genomic investigation of fundamental questions in plant pathology. Arabidopsis thaliana has become the model plant in the past 15 years, and the number of tools available for it imposes its use for the study of fundamental questions in plant biology (Somerville and Meyerowitz, 2004). Numerous studies of plant- pathogen interactions have used this model crucifer and have provided considerable information. Although Pathosystems of Arabidopsis with a large number of viral, bacterial and fungal plant pathogenic micro-organisms have been developed, the Arabidopsis- Pseudomonas syringae ( P. syringae ) system occupies an outstanding position, both because of the large number of groups using it and because of the quantity of exciting insights into fundamental aspects of plant-pathogen interaction it has
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optimization of pathogenicity assays to study the arabidopsis thaliana xanthomonas campestris pv campestris Pathosystem
Molecular Plant Pathology, 2005Co-Authors: Damien F. Meyer, Emmanuelle Lauber, Dominique Roby, Matthieu Arlat, Thomas KrojAbstract:SUMMARY The cruciferous weed Arabidopsis thaliana and the causal agent of black rot disease of Crucifers Xanthomonas campestris pv. campestris ( Xcc ) are both model organisms in plant pathology. Their interaction has been studied successfully in the past, but these investigations suffered from high variability. In the present study, we describe an improved Arabidopsis- Xcc Pathosystem that is based on a wound inoculation procedure. We show that after wound inoculation, Xcc colonizes the vascular system of Arabidopsis leaves and causes typical black rot symptoms in a compatible interaction, while in an incompatible interaction bacterial multiplication is inhibited. The highly synchronous and reproducible symptom expression allowed the development of a disease scoring scheme that enabled us to analyse the effects of mutations in individual genes on plant resistance or on bacterial virulence in a simple and precise manner. This optimized Arabidopsis- Xcc Pathosystem will be a robust tool for further genetic and post-genomic investigation of fundamental questions in plant pathology. Arabidopsis thaliana has become the model plant in the past 15 years, and the number of tools available for it imposes its use for the study of fundamental questions in plant biology (Somerville and Meyerowitz, 2004). Numerous studies of plant- pathogen interactions have used this model crucifer and have provided considerable information. Although Pathosystems of Arabidopsis with a large number of viral, bacterial and fungal plant pathogenic micro-organisms have been developed, the Arabidopsis- Pseudomonas syringae ( P. syringae ) system occupies an outstanding position, both because of the large number of groups using it and because of the quantity of exciting insights into fundamental aspects of plant-pathogen interaction it has
Daniel J Kliebenstein - One of the best experts on this subject based on the ideXlab platform.
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pathogen genetic control of transcriptome variation in the arabidopsis thaliana botrytis cinerea Pathosystem
Genetics, 2020Co-Authors: Nicole E Soltis, Celine Caseys, Wei Zhang, Jason A Corwin, Susanna Atwell, Daniel J KliebensteinAbstract:In plant–pathogen relations, disease symptoms arise from the interaction of the host and pathogen genomes. Host–pathogen functional gene interactions are well described, whereas little is known about how the pathogen genetic variation modulates both organisms’ transcriptomes. To model and generate hypotheses on a generalist pathogen control of gene expression regulation, we used the Arabidopsis thaliana–Botrytis cinerea Pathosystem and the genetic diversity of a collection of 96 B. cinerea isolates. We performed expression-based genome-wide association (eGWA) for each of 23,947 measurable transcripts in Arabidopsis (host), and 9267 measurable transcripts in B. cinerea (pathogen). Unlike other eGWA studies, we detected a relative absence of locally acting expression quantitative trait loci (cis-eQTL), partly caused by structural variants and allelic heterogeneity hindering their identification. This study identified several distantly acting trans-eQTL linked to eQTL hotspots dispersed across Botrytis genome that altered only Botrytis transcripts, only Arabidopsis transcripts, or transcripts from both species. Gene membership in the trans-eQTL hotspots suggests links between gene expression regulation and both known and novel virulence mechanisms in this Pathosystem. Genes annotated to these hotspots provide potential targets for blocking manipulation of the host response by this ubiquitous generalist necrotrophic pathogen.
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Pathogen Genetic Control of Transcriptome Variation in the Arabidopsis thaliana – Botrytis cinerea Pathosystem
Genetics, 2020Co-Authors: Nicole E Soltis, Celine Caseys, Wei Zhang, Jason A Corwin, Susanna Atwell, Daniel J KliebensteinAbstract:In plant–pathogen relations, disease symptoms arise from the interaction of the host and pathogen genomes. Host–pathogen functional gene interactions are well described, whereas little is known about how the pathogen genetic variation modulates both organisms’ transcriptomes. To model and generate hypotheses on a generalist pathogen control of gene expression regulation, we used the Arabidopsis thaliana–Botrytis cinerea Pathosystem and the genetic diversity of a collection of 96 B. cinerea isolates. We performed expression-based genome-wide association (eGWA) for each of 23,947 measurable transcripts in Arabidopsis (host), and 9267 measurable transcripts in B. cinerea (pathogen). Unlike other eGWA studies, we detected a relative absence of locally acting expression quantitative trait loci (cis-eQTL), partly caused by structural variants and allelic heterogeneity hindering their identification. This study identified several distantly acting trans-eQTL linked to eQTL hotspots dispersed across Botrytis genome that altered only Botrytis transcripts, only Arabidopsis transcripts, or transcripts from both species. Gene membership in the trans-eQTL hotspots suggests links between gene expression regulation and both known and novel virulence mechanisms in this Pathosystem. Genes annotated to these hotspots provide potential targets for blocking manipulation of the host response by this ubiquitous generalist necrotrophic pathogen.
Damien F. Meyer - One of the best experts on this subject based on the ideXlab platform.
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Optimization of pathogenicity assays to study the Arabidopsis thaliana–Xanthomonas campestris pv. campestris Pathosystem
Molecular plant pathology, 2005Co-Authors: Damien F. Meyer, Emmanuelle Lauber, Dominique Roby, Matthieu Arlat, Thomas KrojAbstract:SUMMARY The cruciferous weed Arabidopsis thaliana and the causal agent of black rot disease of Crucifers Xanthomonas campestris pv. campestris ( Xcc ) are both model organisms in plant pathology. Their interaction has been studied successfully in the past, but these investigations suffered from high variability. In the present study, we describe an improved Arabidopsis- Xcc Pathosystem that is based on a wound inoculation procedure. We show that after wound inoculation, Xcc colonizes the vascular system of Arabidopsis leaves and causes typical black rot symptoms in a compatible interaction, while in an incompatible interaction bacterial multiplication is inhibited. The highly synchronous and reproducible symptom expression allowed the development of a disease scoring scheme that enabled us to analyse the effects of mutations in individual genes on plant resistance or on bacterial virulence in a simple and precise manner. This optimized Arabidopsis- Xcc Pathosystem will be a robust tool for further genetic and post-genomic investigation of fundamental questions in plant pathology. Arabidopsis thaliana has become the model plant in the past 15 years, and the number of tools available for it imposes its use for the study of fundamental questions in plant biology (Somerville and Meyerowitz, 2004). Numerous studies of plant- pathogen interactions have used this model crucifer and have provided considerable information. Although Pathosystems of Arabidopsis with a large number of viral, bacterial and fungal plant pathogenic micro-organisms have been developed, the Arabidopsis- Pseudomonas syringae ( P. syringae ) system occupies an outstanding position, both because of the large number of groups using it and because of the quantity of exciting insights into fundamental aspects of plant-pathogen interaction it has
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optimization of pathogenicity assays to study the arabidopsis thaliana xanthomonas campestris pv campestris Pathosystem
Molecular Plant Pathology, 2005Co-Authors: Damien F. Meyer, Emmanuelle Lauber, Dominique Roby, Matthieu Arlat, Thomas KrojAbstract:SUMMARY The cruciferous weed Arabidopsis thaliana and the causal agent of black rot disease of Crucifers Xanthomonas campestris pv. campestris ( Xcc ) are both model organisms in plant pathology. Their interaction has been studied successfully in the past, but these investigations suffered from high variability. In the present study, we describe an improved Arabidopsis- Xcc Pathosystem that is based on a wound inoculation procedure. We show that after wound inoculation, Xcc colonizes the vascular system of Arabidopsis leaves and causes typical black rot symptoms in a compatible interaction, while in an incompatible interaction bacterial multiplication is inhibited. The highly synchronous and reproducible symptom expression allowed the development of a disease scoring scheme that enabled us to analyse the effects of mutations in individual genes on plant resistance or on bacterial virulence in a simple and precise manner. This optimized Arabidopsis- Xcc Pathosystem will be a robust tool for further genetic and post-genomic investigation of fundamental questions in plant pathology. Arabidopsis thaliana has become the model plant in the past 15 years, and the number of tools available for it imposes its use for the study of fundamental questions in plant biology (Somerville and Meyerowitz, 2004). Numerous studies of plant- pathogen interactions have used this model crucifer and have provided considerable information. Although Pathosystems of Arabidopsis with a large number of viral, bacterial and fungal plant pathogenic micro-organisms have been developed, the Arabidopsis- Pseudomonas syringae ( P. syringae ) system occupies an outstanding position, both because of the large number of groups using it and because of the quantity of exciting insights into fundamental aspects of plant-pathogen interaction it has
Claire Campion - One of the best experts on this subject based on the ideXlab platform.
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The Arabidopsis thaliana-Alternaria brassicicola Pathosystem: A model interaction for investigating seed transmission of necrotrophic fungi
Plant methods, 2012Co-Authors: Stéphanie Pochon, Emmanuel Terrasson, Thomas Guillemette, Beatrice Iacomi-vasilescu, Sonia Georgeault, Marjorie Juchaux, Romain Berruyer, Isabelle Debeaujon, Philippe Simoneau, Claire CampionAbstract:Background: Seed transmission constitutes a major component of the parasitic cycle for several fungal pathogens. However, very little is known concerning fungal or plant genetic factors that impact seed transmission and mechanisms underlying this key biological trait have yet to be clarified. Such lack of available data could be probably explained by the absence of suitable model Pathosystem to study plant-fungus interactions during the plant reproductive phase. Results: Here we report on setting up a new Pathosystem that could facilitate the study of fungal seed transmission. Reproductive organs of Arabidopsis thaliana were inoculated with Alternaria brassicicola conidia. Parameters (floral vs fruit route, seed collection date, plant and silique developmental stages) that could influence the seed transmission efficiency were tested to define optimal seed infection conditions. Microscopic observations revealed that the fungus penetrates siliques through cellular junctions, replum and stomata, and into seed coats either directly or through cracks. The ability of the osmosensitive fungal mutant nik1Δ3 to transmit to A. thaliana seeds was analyzed. A significant decrease in seed transmission rate was observed compared to the wild-type parental strain, confirming that a functional osmoregulation pathway is required for efficient seed transmission of the fungus. Similarly, to test the role of flavonoids in seed coat protection against pathogens, a transparent testa Arabidopsis mutant (tt4-1 )n ot producing any flavonoid was used as host plant. Unexpectedly, tt4-1 seeds were infected to a significantly lower extent than wild-type seeds, possibly due to over-accumulation of other antimicrobial metabolites. Conclusions: The Arabidopsis thaliana-Alternaria brassicicola Pathosystem, that have been widely used to study plant-pathogen interactions during the vegetative phase, also proved to constitute a suitable model Pathosystem for detailed analysis of plant-pathogen interactions during the reproductive phase. We demonstrated that it provides an excellent system for investigating the impact of different fungal or plant mutations on the seed transmission process and therefore paves the way towards future high-throughput screening of both Arabidopsis and fungal mutant.
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The Arabidopsis thaliana-Alternaria brassicicola Pathosystem: A model interaction for investigating seed transmission of necrotrophic fungi
Plant Methods, 2012Co-Authors: Stéphanie Pochon, Emmanuel Terrasson, Thomas Guillemette, Beatrice Iacomi-vasilescu, Sonia Georgeault, Marjorie Juchaux, Romain Berruyer, Isabelle Debeaujon, Philippe Simoneau, Claire CampionAbstract:Background: Seed transmission constitutes a major component of the parasitic cycle for several fungal pathogens. However, very little is known concerning fungal or plant genetic factors that impact seed transmission and mechanisms underlying this key biological trait have yet to be clarified. Such lack of available data could be probably explained by the absence of suitable model Pathosystem to study plant-fungus interactions during the plant reproductive phase. Results: Here we report on setting up a new Pathosystem that could facilitate the study of fungal seed transmission. Reproductive organs of Arabidopsis thaliana were inoculated with Alternaria brassicicola conidia. Parameters ( floral vs fruit route, seed collection date, plant and silique developmental stages) that could influence the seed transmission efficiency were tested to define optimal seed infection conditions. Microscopic observations revealed that the fungus penetrates siliques through cellular junctions, replum and stomata, and into seed coats either directly or through cracks. The ability of the osmosensitive fungal mutant nik1 Delta 3 to transmit to A. thaliana seeds was analyzed. A significant decrease in seed transmission rate was observed compared to the wild-type parental strain, confirming that a functional osmoregulation pathway is required for efficient seed transmission of the fungus. Similarly, to test the role of flavonoids in seed coat protection against pathogens, a transparent testa Arabidopsis mutant (tt4-1) not producing any flavonoid was used as host plant. Unexpectedly, tt4-1 seeds were infected to a significantly lower extent than wild-type seeds, possibly due to over-accumulation of other antimicrobial metabolites. Conclusions: The Arabidopsis thaliana-Alternaria brassicicola Pathosystem, that have been widely used to study plant-pathogen interactions during the vegetative phase, also proved to constitute a suitable model Pathosystem for detailed analysis of plant-pathogen interactions during the reproductive phase. We demonstrated that it provides an excellent system for investigating the impact of different fungal or plant mutations on the seed transmission process and therefore paves the way towards future high-throughput screening of both Arabidopsis and fungal mutant.
Guo Liang Wang - One of the best experts on this subject based on the ideXlab platform.
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recent progress in understanding pamp and effector triggered immunity against the rice blast fungus magnaporthe oryzae
Molecular Plant, 2013Co-Authors: Yuese Ning, Zhilong Wang, Xuli Wang, Bo Ding, Guo Liang WangAbstract:ABSTRACT Rice blast, caused by the fungal pathogen Magnaporthe oryzae , is one of the most destructive diseases of rice worldwide. The rice– M. oryzae Pathosystem has become a model in the study of plant–fungal interactions because of its scientific advancement and economic importance. Recent studies have identified a number of new pathogen-associated molecular patterns (PAMPs) and effectors from the blast fungus that trigger rice immune responses upon perception. Interaction analyses between avirulence effectors and their cognate resistance proteins have provided new insights into the molecular basis of plant–fungal interactions. In this review, we summarize the recent research on the characterization of those genes in both M. oryzae and rice that are important for the PAMP- and effector-triggered immunity recognition and signaling processes. We also discuss future directions for research that will further our understanding of this Pathosystem. This review focuses on the recent advances in studies of rice innate immunity against the fungal pathogen Magnaporthe oryzae . The functions of PAMP and avirulence effectors in M. oryzae and their host targets and receptors in rice are summarized.
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recent progress in understanding pamp and effector triggered immunity against the rice blast fungus magnaporthe oryzae
Molecular Plant, 2013Co-Authors: Wende Liu, Yuese Ning, Zhilong Wang, Xuli Wang, Bo Ding, Jinling Liu, Guo Liang WangAbstract:Rice blast, caused by the fungal pathogen Magnaporthe oryzae, is one of the most destructive diseases of rice worldwide. The rice-M. oryzae Pathosystem has become a model in the study of plant-fungal interactions because of its scientific advancement and economic importance. Recent studies have identified a number of new pathogen-associated molecular patterns (PAMPs) and effectors from the blast fungus that trigger rice immune responses upon perception. Interaction analyses between avirulence effectors and their cognate resistance proteins have provided new insights into the molecular basis of plant-fungal interactions. In this review, we summarize the recent research on the characterization of those genes in both M. oryzae and rice that are important for the PAMP- and effector-triggered immunity recognition and signaling processes. We also discuss future directions for research that will further our understanding of this Pathosystem.
