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Achille P Caputi - One of the best experts on this subject based on the ideXlab platform.

  • protective effects of poly adp ribose synthase inhibitors in zymosan activated plasma induced Paw edema
    Life Sciences, 1999
    Co-Authors: Salvatore Cuzzocrea, Basilia Zingarelli, Giuseppina Costantino, Achille P Caputi
    Abstract:

    Abstract The aim of the present study was to investigate the role of poly (ADP-ribose) synthetase (PARS) in a model of acute local inflammation (zymosan-activated plasma (ZAP)-induced Paw edema), in which the oxyradicals, nitric oxide and peroxynitrite, are known to play a crucial role. Injection of zymosan-activated plasma (ZAP) into the rat Paw induced edema formation. The maximal increase in Paw volume was observed at three hours after administration (maximal in Paw volume: 1.29 ± 0.09 ml).At this time point, there was a marked increase in neutrophil infiltration in the Paw, as measured by an increase in myeloperoxidase (MPO) activity in the Paw tissue (260 ± 25 mU 100 mg wet tissue). However, ZAP-induced Paw edema was significantly reduced in a dosedependent manner by treatment with 3-aminobenzamide (3-AB) or nicotinamide (NIC), two inhibitors of PARS, at 1, 2,3,4 hours after ZAP injection. PARS inhibition also caused a significant reduction of MPO activity. The Paw tissues were also examined immunohistochemically for the presence of nitrotyrosine (a footprint for peroxynitrite formation). At 3h following ZAP injection, staining for nitrotyrosine were also found to be localised within discrete cells in the inflamed Paw tissue. Treatment with PARS inhibitor prevented the appearance of nitrotyrosine in the tissues. Our results suggest that in Paw edema induced by ZAP, inhibition of PARS exert potent antiinflammatory effects.

  • Protective effects of melatonin in zymosan-activated plasma-induced Paw inflammation
    European journal of pharmacology, 1998
    Co-Authors: Giuseppina Costantino, Salvatore Cuzzocrea, Emanuela Mazzon, Achille P Caputi
    Abstract:

    The aim of the present study was to investigate the protective effect of the pineal hormone melatonin in a model of acute local inflammation (zymosan-activated plasma-induced Paw oedema), in which oxyradicals, nitric oxide (NO) and peroxynitrite are known to play a crucial role in the inflammatory process. The intraplantar injection of zymosan-activated plasma elicited an inflammatory response that was characterized by a time-dependent increase in Paw oedema, neutrophil infiltration and increased levels of nitrite/nitrate in the Paw exudate. The maximal increase in Paw volume was observed at 3 h after administration (maximal in Paw volume: 1.34 +/- 0.09 ml). At this time point, myeloperoxidase activity and lipid peroxidation were markedly increased in the zymosan-activated plasma-treated Paw (226 +/- 10.2 mU/100 mg wet tissue, 31 +/- 2.1 mM/mg wet tissue, respectively). However, zymosan-activated plasma-induced Paw oedema was significantly reduced in a dose-dependent manner by treatment with melatonin (given at 62.5 and 125 microg/Paw) at 1, 2, 3, 4 h after injection of zymosan-activated plasma. Melatonin treatment also caused a significant reduction of the myeloperoxidase activity and lipid peroxidation and inhibited nitrite/nitrate levels in the Paw exudate. The Paw tissues were also examined immunohistochemically for the presence of nitrotyrosine (a marker of peroxynitrite formation). At 3 h following injection of zymosan-activated plasma, staining for nitrotyrosine was also found to be localised in the inflamed Paw tissue. Treatment with melatonin (125 microg/Paw) reduced the appearance of nitrotyrosine in the tissues. Our findings support the view that melatonin exerts anti-inflammatory effects.

Giuseppina Costantino - One of the best experts on this subject based on the ideXlab platform.

  • protective effects of poly adp ribose synthase inhibitors in zymosan activated plasma induced Paw edema
    Life Sciences, 1999
    Co-Authors: Salvatore Cuzzocrea, Basilia Zingarelli, Giuseppina Costantino, Achille P Caputi
    Abstract:

    Abstract The aim of the present study was to investigate the role of poly (ADP-ribose) synthetase (PARS) in a model of acute local inflammation (zymosan-activated plasma (ZAP)-induced Paw edema), in which the oxyradicals, nitric oxide and peroxynitrite, are known to play a crucial role. Injection of zymosan-activated plasma (ZAP) into the rat Paw induced edema formation. The maximal increase in Paw volume was observed at three hours after administration (maximal in Paw volume: 1.29 ± 0.09 ml).At this time point, there was a marked increase in neutrophil infiltration in the Paw, as measured by an increase in myeloperoxidase (MPO) activity in the Paw tissue (260 ± 25 mU 100 mg wet tissue). However, ZAP-induced Paw edema was significantly reduced in a dosedependent manner by treatment with 3-aminobenzamide (3-AB) or nicotinamide (NIC), two inhibitors of PARS, at 1, 2,3,4 hours after ZAP injection. PARS inhibition also caused a significant reduction of MPO activity. The Paw tissues were also examined immunohistochemically for the presence of nitrotyrosine (a footprint for peroxynitrite formation). At 3h following ZAP injection, staining for nitrotyrosine were also found to be localised within discrete cells in the inflamed Paw tissue. Treatment with PARS inhibitor prevented the appearance of nitrotyrosine in the tissues. Our results suggest that in Paw edema induced by ZAP, inhibition of PARS exert potent antiinflammatory effects.

  • Protective effects of melatonin in zymosan-activated plasma-induced Paw inflammation
    European journal of pharmacology, 1998
    Co-Authors: Giuseppina Costantino, Salvatore Cuzzocrea, Emanuela Mazzon, Achille P Caputi
    Abstract:

    The aim of the present study was to investigate the protective effect of the pineal hormone melatonin in a model of acute local inflammation (zymosan-activated plasma-induced Paw oedema), in which oxyradicals, nitric oxide (NO) and peroxynitrite are known to play a crucial role in the inflammatory process. The intraplantar injection of zymosan-activated plasma elicited an inflammatory response that was characterized by a time-dependent increase in Paw oedema, neutrophil infiltration and increased levels of nitrite/nitrate in the Paw exudate. The maximal increase in Paw volume was observed at 3 h after administration (maximal in Paw volume: 1.34 +/- 0.09 ml). At this time point, myeloperoxidase activity and lipid peroxidation were markedly increased in the zymosan-activated plasma-treated Paw (226 +/- 10.2 mU/100 mg wet tissue, 31 +/- 2.1 mM/mg wet tissue, respectively). However, zymosan-activated plasma-induced Paw oedema was significantly reduced in a dose-dependent manner by treatment with melatonin (given at 62.5 and 125 microg/Paw) at 1, 2, 3, 4 h after injection of zymosan-activated plasma. Melatonin treatment also caused a significant reduction of the myeloperoxidase activity and lipid peroxidation and inhibited nitrite/nitrate levels in the Paw exudate. The Paw tissues were also examined immunohistochemically for the presence of nitrotyrosine (a marker of peroxynitrite formation). At 3 h following injection of zymosan-activated plasma, staining for nitrotyrosine was also found to be localised in the inflamed Paw tissue. Treatment with melatonin (125 microg/Paw) reduced the appearance of nitrotyrosine in the tissues. Our findings support the view that melatonin exerts anti-inflammatory effects.

Salvatore Cuzzocrea - One of the best experts on this subject based on the ideXlab platform.

  • protective effects of poly adp ribose synthase inhibitors in zymosan activated plasma induced Paw edema
    Life Sciences, 1999
    Co-Authors: Salvatore Cuzzocrea, Basilia Zingarelli, Giuseppina Costantino, Achille P Caputi
    Abstract:

    Abstract The aim of the present study was to investigate the role of poly (ADP-ribose) synthetase (PARS) in a model of acute local inflammation (zymosan-activated plasma (ZAP)-induced Paw edema), in which the oxyradicals, nitric oxide and peroxynitrite, are known to play a crucial role. Injection of zymosan-activated plasma (ZAP) into the rat Paw induced edema formation. The maximal increase in Paw volume was observed at three hours after administration (maximal in Paw volume: 1.29 ± 0.09 ml).At this time point, there was a marked increase in neutrophil infiltration in the Paw, as measured by an increase in myeloperoxidase (MPO) activity in the Paw tissue (260 ± 25 mU 100 mg wet tissue). However, ZAP-induced Paw edema was significantly reduced in a dosedependent manner by treatment with 3-aminobenzamide (3-AB) or nicotinamide (NIC), two inhibitors of PARS, at 1, 2,3,4 hours after ZAP injection. PARS inhibition also caused a significant reduction of MPO activity. The Paw tissues were also examined immunohistochemically for the presence of nitrotyrosine (a footprint for peroxynitrite formation). At 3h following ZAP injection, staining for nitrotyrosine were also found to be localised within discrete cells in the inflamed Paw tissue. Treatment with PARS inhibitor prevented the appearance of nitrotyrosine in the tissues. Our results suggest that in Paw edema induced by ZAP, inhibition of PARS exert potent antiinflammatory effects.

  • Protective effects of melatonin in zymosan-activated plasma-induced Paw inflammation
    European journal of pharmacology, 1998
    Co-Authors: Giuseppina Costantino, Salvatore Cuzzocrea, Emanuela Mazzon, Achille P Caputi
    Abstract:

    The aim of the present study was to investigate the protective effect of the pineal hormone melatonin in a model of acute local inflammation (zymosan-activated plasma-induced Paw oedema), in which oxyradicals, nitric oxide (NO) and peroxynitrite are known to play a crucial role in the inflammatory process. The intraplantar injection of zymosan-activated plasma elicited an inflammatory response that was characterized by a time-dependent increase in Paw oedema, neutrophil infiltration and increased levels of nitrite/nitrate in the Paw exudate. The maximal increase in Paw volume was observed at 3 h after administration (maximal in Paw volume: 1.34 +/- 0.09 ml). At this time point, myeloperoxidase activity and lipid peroxidation were markedly increased in the zymosan-activated plasma-treated Paw (226 +/- 10.2 mU/100 mg wet tissue, 31 +/- 2.1 mM/mg wet tissue, respectively). However, zymosan-activated plasma-induced Paw oedema was significantly reduced in a dose-dependent manner by treatment with melatonin (given at 62.5 and 125 microg/Paw) at 1, 2, 3, 4 h after injection of zymosan-activated plasma. Melatonin treatment also caused a significant reduction of the myeloperoxidase activity and lipid peroxidation and inhibited nitrite/nitrate levels in the Paw exudate. The Paw tissues were also examined immunohistochemically for the presence of nitrotyrosine (a marker of peroxynitrite formation). At 3 h following injection of zymosan-activated plasma, staining for nitrotyrosine was also found to be localised in the inflamed Paw tissue. Treatment with melatonin (125 microg/Paw) reduced the appearance of nitrotyrosine in the tissues. Our findings support the view that melatonin exerts anti-inflammatory effects.

Daniel Neuhauser - One of the best experts on this subject based on the ideXlab platform.

  • real space orthogonal projector augmented wave method
    Physical Review B, 2020
    Co-Authors: Daniel Neuhauser
    Abstract:

    The projector-augmented-wave (Paw) method of Bl\"ochl makes smooth but nonorthogonal orbitals. Here we show how to make a Paw orthogonal using a cheap transformation of the wave functions. We show that the resulting orthogonal Paw (OPaw), applied for density functional theory, reproduces (for a large variety of solids) band gaps from the abinit package. OPaw combines the underlying orthogonality of norm-conserving pseudopotentials with the large grid spacings and small energy cutoffs in Paw. The OPaw framework can also be combined with other electronic structure theory methods.

Mi-song Dupuy - One of the best experts on this subject based on the ideXlab platform.

  • Projector augmented-wave method: an analysis in a one-dimensional setting
    ESAIM: Mathematical Modelling and Numerical Analysis, 2020
    Co-Authors: Mi-song Dupuy
    Abstract:

    In this article, a numerical analysis of the projector augmented-wave (Paw) method is presented, restricted to the case of dimension one with Dirac potentials modeling the nuclei in a periodic setting. The Paw method is widely used in electronic ab initio calculations, in conjunction with pseudopotentials. It consists in replacing the original electronic Hamiltonian by a pseudo-Hamiltonian Paw via the Paw transformation acting in balls around each nuclei. Formally, the new eigenvalue problem has the same eigenvalues as and smoother eigenfunctions. In practice, the pseudo-Hamiltonian Paw has to be truncated, introducing an error that is rarely analyzed. In this paper, error estimates on the lowest Paw eigenvalue are proved for the one-dimensional periodic Schrödinger operator with double Dirac potentials.