The Experts below are selected from a list of 132 Experts worldwide ranked by ideXlab platform
G R Wang - One of the best experts on this subject based on the ideXlab platform.
-
α terthienyl photosensitizes damage to PBR322 dna
Photochemistry and Photobiology, 1991Co-Authors: Tian P Wang, Jacques Kagan, R W Tuveson, G R WangAbstract:: alpha-Terthienyl photosensitizes single strand breaks in PBR322 DNA. Almost identical results were observed under oxygen and under argon. In the presence of oxygen, this DNA nicking was enhanced by histidine and was not affected by superoxide dismutase, catalase, or the antioxidant BHT. Although chemical damage to DNA treated with alpha-terthienyl plus near-UV was clearly demonstrated in vitro, transformation in E. coli with this damaged PBR322 DNA still took place. Likewise, Haemophilus influenzae DNA transforming activity was not significantly decreased by photosensitization with alpha-terthienyl.
-
α‐TERTHIENYL PHOTOSENSITIZES DAMAGE TO PBR322 DNA
Photochemistry and Photobiology, 1991Co-Authors: Tian P Wang, Jacques Kagan, R W Tuveson, G R WangAbstract:: alpha-Terthienyl photosensitizes single strand breaks in PBR322 DNA. Almost identical results were observed under oxygen and under argon. In the presence of oxygen, this DNA nicking was enhanced by histidine and was not affected by superoxide dismutase, catalase, or the antioxidant BHT. Although chemical damage to DNA treated with alpha-terthienyl plus near-UV was clearly demonstrated in vitro, transformation in E. coli with this damaged PBR322 DNA still took place. Likewise, Haemophilus influenzae DNA transforming activity was not significantly decreased by photosensitization with alpha-terthienyl.
G G Schmitz - One of the best experts on this subject based on the ideXlab platform.
-
swal a unique restriction endonuclease from staphylococcus warneri which recognizes 5 atttaaat 3
Nucleic Acids Research, 1992Co-Authors: M Lechner, J Antonbotella, Cassandra L Smith, Waltraud Ankenbauer, B. Frey, Frank Laue, G G SchmitzAbstract:: A novel class-II restriction endonuclease designated SwaI was purified from Staphylococcus warneri. This enzyme cleaves adenovirus 2 DNA, SV40 DNA and M13mp7 at one site each, but does not cleave lambda, PhiX174, PBR322 or pBR328 DNA. SwaI recognizes the octanucleotide sequence 5'-ATTTAAAT-3', cleaving in the center of the recognition sequence creating blunt ended DNA fragments. SwaI was used to digest chromosomal DNA from various microorganisms and human cells.
-
Swal, a unique restriction endonuclease from Staphylococcus warneri, which recognizes 5′-ATTTAAAT-3′
Nucleic Acids Research, 1992Co-Authors: M Lechner, Cassandra L Smith, Waltraud Ankenbauer, J. Anton-botella, B. Frey, Frank Laue, G G SchmitzAbstract:: A novel class-II restriction endonuclease designated SwaI was purified from Staphylococcus warneri. This enzyme cleaves adenovirus 2 DNA, SV40 DNA and M13mp7 at one site each, but does not cleave lambda, PhiX174, PBR322 or pBR328 DNA. SwaI recognizes the octanucleotide sequence 5'-ATTTAAAT-3', cleaving in the center of the recognition sequence creating blunt ended DNA fragments. SwaI was used to digest chromosomal DNA from various microorganisms and human cells.
Tian P Wang - One of the best experts on this subject based on the ideXlab platform.
-
α terthienyl photosensitizes damage to PBR322 dna
Photochemistry and Photobiology, 1991Co-Authors: Tian P Wang, Jacques Kagan, R W Tuveson, G R WangAbstract:: alpha-Terthienyl photosensitizes single strand breaks in PBR322 DNA. Almost identical results were observed under oxygen and under argon. In the presence of oxygen, this DNA nicking was enhanced by histidine and was not affected by superoxide dismutase, catalase, or the antioxidant BHT. Although chemical damage to DNA treated with alpha-terthienyl plus near-UV was clearly demonstrated in vitro, transformation in E. coli with this damaged PBR322 DNA still took place. Likewise, Haemophilus influenzae DNA transforming activity was not significantly decreased by photosensitization with alpha-terthienyl.
-
α‐TERTHIENYL PHOTOSENSITIZES DAMAGE TO PBR322 DNA
Photochemistry and Photobiology, 1991Co-Authors: Tian P Wang, Jacques Kagan, R W Tuveson, G R WangAbstract:: alpha-Terthienyl photosensitizes single strand breaks in PBR322 DNA. Almost identical results were observed under oxygen and under argon. In the presence of oxygen, this DNA nicking was enhanced by histidine and was not affected by superoxide dismutase, catalase, or the antioxidant BHT. Although chemical damage to DNA treated with alpha-terthienyl plus near-UV was clearly demonstrated in vitro, transformation in E. coli with this damaged PBR322 DNA still took place. Likewise, Haemophilus influenzae DNA transforming activity was not significantly decreased by photosensitization with alpha-terthienyl.
Venugopal P. Menon - One of the best experts on this subject based on the ideXlab platform.
-
Evaluation of antioxidant activity of hesperidin and its protective effect on H_2O_2 induced oxidative damage on PBR322 DNA and RBC cellular membrane
Molecular and Cellular Biochemistry, 2009Co-Authors: K. B. Kalpana, M. Srinivasan, Venugopal P. MenonAbstract:The present work was carried out to evaluate the antioxidant activity of hesperidin and to study its protective effect on H_2O_2 induced oxidative damage on PBR322 DNA and RBC cellular membrane. The in vitro assays were performed with different concentrations (2, 4, 6, 8, and 10 μg/ml, which were equivalent to 3.27, 6.55, 9.83, 13.10, and 16.38 μM) of hesperidin and the results clearly indicate that hesperidin at 10 μg/ml exhibited radical scavenging activity greater than that of standards like ascorbic acid and trolox. The protective effect of hesperidin on PBR322 DNA and RBC cellular membrane on treatment with different concentrations of H_2O_2 shows that hesperidin at 2.5 mM converts the open circular form (oc) of PBR322 DNA that is an indication of damage to super coiled (ccc) form and at 10 μg/ml it prevents membrane damage. Thus, our result proves hesperidin to be a valuable antioxidant that protects PBR322 DNA and RBC cellular membrane from free radical induced oxidative damage.
-
evaluation of antioxidant activity of hesperidin and its protective effect on h2o2 induced oxidative damage on PBR322 dna and rbc cellular membrane
Molecular and Cellular Biochemistry, 2009Co-Authors: K. B. Kalpana, M. Srinivasan, Venugopal P. MenonAbstract:The present work was carried out to evaluate the antioxidant activity of hesperidin and to study its protective effect on H2O2 induced oxidative damage on PBR322 DNA and RBC cellular membrane. The in vitro assays were performed with different concentrations (2, 4, 6, 8, and 10 μg/ml, which were equivalent to 3.27, 6.55, 9.83, 13.10, and 16.38 μM) of hesperidin and the results clearly indicate that hesperidin at 10 μg/ml exhibited radical scavenging activity greater than that of standards like ascorbic acid and trolox. The protective effect of hesperidin on PBR322 DNA and RBC cellular membrane on treatment with different concentrations of H2O2 shows that hesperidin at 2.5 mM converts the open circular form (oc) of PBR322 DNA that is an indication of damage to super coiled (ccc) form and at 10 μg/ml it prevents membrane damage. Thus, our result proves hesperidin to be a valuable antioxidant that protects PBR322 DNA and RBC cellular membrane from free radical induced oxidative damage.
-
antioxidant potential of aminothiazole derivative and its protective effect on h2o2 induced oxidative damage on PBR322 dna and rbc cellular membrane
Molecular and Cellular Biochemistry, 2008Co-Authors: K. B. Kalpana, M. Srinivasan, Venugopal P. MenonAbstract:The present work was carried out to evaluate the antioxidant and free radical scavenging activity of aminothiazole derivative by performing various in vitro assays; to study its protective effect on H2O2-induced oxidative damage on PBR322 DNA and on RBC cellular membrane. The in vitro assays were performed with different concentrations of aminothiazole derivative (6.15, 12.29, 18.44, 24.59, and 30.73 μM) and the results were compared with standards like ascorbic acid and trolox. Our results clearly indicated that aminothiazole derivative at a dose of 18.44 μM exhibited radical scavenging activity greater than that of ascorbic acid and trolox. The DNA protective effect on PBR322 DNA showed that there was a concentration-dependent inhibition of the disappearance of supercoiled (ccc) form of DNA on incubation with 30 mM H2O2 in the presence of different concentrations of aminothiazole derivative. Thus our compound at 1.5 mM prevents the conversion from supercoiled (ccc) form to open circular form (oc) form of PBR322 DNA. Pretreatment with aminothiazole derivative at a dose of 18.44 μM prevents membrane damage and exhibits an IC50 value, which is the concentration of the sample required to inhibit 50% of the radical formed greater than that of the standards (ascorbic acid and trolox). Thus our compound of interest aminothiazole derivative exhibits antioxidant and free radical scavenging properties greater than that of standards like ascorbic acid and trolox and thereby protects PBR322 DNA and RBC cellular membrane from free radical induced oxidative damage.
M Lechner - One of the best experts on this subject based on the ideXlab platform.
-
swal a unique restriction endonuclease from staphylococcus warneri which recognizes 5 atttaaat 3
Nucleic Acids Research, 1992Co-Authors: M Lechner, J Antonbotella, Cassandra L Smith, Waltraud Ankenbauer, B. Frey, Frank Laue, G G SchmitzAbstract:: A novel class-II restriction endonuclease designated SwaI was purified from Staphylococcus warneri. This enzyme cleaves adenovirus 2 DNA, SV40 DNA and M13mp7 at one site each, but does not cleave lambda, PhiX174, PBR322 or pBR328 DNA. SwaI recognizes the octanucleotide sequence 5'-ATTTAAAT-3', cleaving in the center of the recognition sequence creating blunt ended DNA fragments. SwaI was used to digest chromosomal DNA from various microorganisms and human cells.
-
Swal, a unique restriction endonuclease from Staphylococcus warneri, which recognizes 5′-ATTTAAAT-3′
Nucleic Acids Research, 1992Co-Authors: M Lechner, Cassandra L Smith, Waltraud Ankenbauer, J. Anton-botella, B. Frey, Frank Laue, G G SchmitzAbstract:: A novel class-II restriction endonuclease designated SwaI was purified from Staphylococcus warneri. This enzyme cleaves adenovirus 2 DNA, SV40 DNA and M13mp7 at one site each, but does not cleave lambda, PhiX174, PBR322 or pBR328 DNA. SwaI recognizes the octanucleotide sequence 5'-ATTTAAAT-3', cleaving in the center of the recognition sequence creating blunt ended DNA fragments. SwaI was used to digest chromosomal DNA from various microorganisms and human cells.
