The Experts below are selected from a list of 321 Experts worldwide ranked by ideXlab platform
Zebin Shi - One of the best experts on this subject based on the ideXlab platform.
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Article Genome-Wide Analysis of Sorbitol Dehydrogenase (SDH) Genes and Their Differential Expression in Two Sand Pear (Pyrus pyrifolia) Fruits
2016Co-Authors: Meisong Dai, Zebin ShiAbstract:Abstract: Through RNA-seq of a mixed fruit sample, fourteen expressed sorbitol dehydrogenase (SDH) genes have been identified from sand Pear (Pyrus pyrifolia Nakai). Comparative phylogenetic analysis of these PpySDHs with those from other plants supported the closest relationship of sand Pear with Chinese white Pear (P. bretschneideri). The expression levels varied greatly among members, and the strongest six (PpySDH2, PpySDH4, PpySDH8, PpySDH12, PpySDH13 and PpySDH14) accounted for 96 % of total transcript abundance of PpySDHs. Tissue-specific expression of these six members was observed in nine tissues or organs of sand Pear, with the greatest abundance found in functional leaf petioles, followed by the flesh of young fruit. Expression patterns of these six PpySDH genes during fruit development were analyzed in two sand Pear cultivars, “Cuiguan ” and “Cuiyu”. Overall, expression of PpySDHs peaked twice, first at the fruitlet stage and again at or near harvest. The transcript abundance of PpySDHs was higher in “Cuiguan ” than in “Cuiyu”, accompanied by a higher content of sugars and higher ratio of fructose to sorbitol maintained in the former cultivar at harvest. In conclusion, it wa
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genome wide analysis of sorbitol dehydrogenase sdh genes and their differential expression in two sand Pear pyrus pyrifolia fruits
International Journal of Molecular Sciences, 2015Co-Authors: Meisong Dai, Zebin ShiAbstract:Through RNA-seq of a mixed fruit sample, fourteen expressed sorbitol dehydrogenase (SDH) genes have been identified from sand Pear (Pyrus pyrifolia Nakai). Comparative phylogenetic analysis of these PpySDHs with those from other plants supported the closest relationship of sand Pear with Chinese white Pear (P. bretschneideri). The expression levels varied greatly among members, and the strongest six (PpySDH2, PpySDH4, PpySDH8, PpySDH12, PpySDH13 and PpySDH14) accounted for 96% of total transcript abundance of PpySDHs. Tissue-specific expression of these six members was observed in nine tissues or organs of sand Pear, with the greatest abundance found in functional leaf petioles, followed by the flesh of young fruit. Expression patterns of these six PpySDH genes during fruit development were analyzed in two sand Pear cultivars, “Cuiguan” and “Cuiyu”. Overall, expression of PpySDHs peaked twice, first at the fruitlet stage and again at or near harvest. The transcript abundance of PpySDHs was higher in “Cuiguan” than in “Cuiyu”, accompanied by a higher content of sugars and higher ratio of fructose to sorbitol maintained in the former cultivar at harvest. In conclusion, it was suggested that multiple members of the SDH gene family are possibly involved in sand Pear fruit development and sugar accumulation and may affect both the sugar amount and sugar composition.
Bruce C Kirkpatrick - One of the best experts on this subject based on the ideXlab platform.
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identification of phytoplasma taxa and insect vectors of peach yellow leaf roll disease in california
Plant Disease, 2002Co-Authors: C L Blomquist, Bruce C KirkpatrickAbstract:ABSTRACT Two peach diseases in California, western X-disease (WX) and peach yellow leaf roll (PYLR) are caused by two genetically distinct phytoplasmas. Based on symptoms alone, WX and PYLR cannot be reliably distinguished. The objectives of this study were to determine which phytoplasma was causing PYLR disease in peach orchards planted near Pear orchards and which phytoplasmas were present in potential insect vectors captured in both peach and Pear orchards. Leafhoppers and Pear psylla were collected from yellow sticky traps placed in three Pear orchards and four peach orchards located in the Sacramento Valley from 1994 to 1996. DNA was extracted from potential vectors and suspect diseased trees, and analyzed for the presence of the WX and PYLR phytoplasmas using strain-specific DNA hybridization or polymerase chain reaction (PCR) assays. The most abundant phytoplasma detected in diseased peach trees was the PYLR phytoplasma. In 1994 and 1995, 10 to 25% of groups of Pear psylla tested positive for the P...
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identification of phytoplasma taxa and insect vectors of peach yellow leaf roll disease in california
Plant Disease, 2002Co-Authors: C L Blomquist, Bruce C KirkpatrickAbstract:Two peach diseases in California, western X-disease (WX) and peach yellow leaf roll (PYLR) are caused by two genetically distinct phytoplasmas. Based on symptoms alone, WX and PYLR cannot be reliably distinguished. The objectives of this study were to determine which phytoplasma was causing PYLR disease in peach orchards planted near Pear orchards and which phytoplasmas were present in potential insect vectors captured in both peach and Pear orchards. Leafhoppers and Pear psylla were collected from yellow sticky traps placed in three Pear orchards and four peach orchards located in the Sacramento Valley from 1994 to 1996. DNA was extracted from potential vectors and suspect diseased trees, and analyzed for the presence of the WX and PYLR phytoplasmas using strain-specific DNA hybridization or polymerase chain reaction (PCR) assays. The most abundant phytoplasma detected in diseased peach trees was the PYLR phytoplasma. In 1994 and 1995, 10 to 25% of groups of Pear psylla tested positive for the PYLR phytoplasma, while no psylla groups tested positive for the WX phytoplasma. Only one captured leafhopper tested positive for the WX phytoplasma. These results indicate the Pear psylla is the primary vector of PYLR in northern California.
Meisong Dai - One of the best experts on this subject based on the ideXlab platform.
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Article Genome-Wide Analysis of Sorbitol Dehydrogenase (SDH) Genes and Their Differential Expression in Two Sand Pear (Pyrus pyrifolia) Fruits
2016Co-Authors: Meisong Dai, Zebin ShiAbstract:Abstract: Through RNA-seq of a mixed fruit sample, fourteen expressed sorbitol dehydrogenase (SDH) genes have been identified from sand Pear (Pyrus pyrifolia Nakai). Comparative phylogenetic analysis of these PpySDHs with those from other plants supported the closest relationship of sand Pear with Chinese white Pear (P. bretschneideri). The expression levels varied greatly among members, and the strongest six (PpySDH2, PpySDH4, PpySDH8, PpySDH12, PpySDH13 and PpySDH14) accounted for 96 % of total transcript abundance of PpySDHs. Tissue-specific expression of these six members was observed in nine tissues or organs of sand Pear, with the greatest abundance found in functional leaf petioles, followed by the flesh of young fruit. Expression patterns of these six PpySDH genes during fruit development were analyzed in two sand Pear cultivars, “Cuiguan ” and “Cuiyu”. Overall, expression of PpySDHs peaked twice, first at the fruitlet stage and again at or near harvest. The transcript abundance of PpySDHs was higher in “Cuiguan ” than in “Cuiyu”, accompanied by a higher content of sugars and higher ratio of fructose to sorbitol maintained in the former cultivar at harvest. In conclusion, it wa
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genome wide analysis of sorbitol dehydrogenase sdh genes and their differential expression in two sand Pear pyrus pyrifolia fruits
International Journal of Molecular Sciences, 2015Co-Authors: Meisong Dai, Zebin ShiAbstract:Through RNA-seq of a mixed fruit sample, fourteen expressed sorbitol dehydrogenase (SDH) genes have been identified from sand Pear (Pyrus pyrifolia Nakai). Comparative phylogenetic analysis of these PpySDHs with those from other plants supported the closest relationship of sand Pear with Chinese white Pear (P. bretschneideri). The expression levels varied greatly among members, and the strongest six (PpySDH2, PpySDH4, PpySDH8, PpySDH12, PpySDH13 and PpySDH14) accounted for 96% of total transcript abundance of PpySDHs. Tissue-specific expression of these six members was observed in nine tissues or organs of sand Pear, with the greatest abundance found in functional leaf petioles, followed by the flesh of young fruit. Expression patterns of these six PpySDH genes during fruit development were analyzed in two sand Pear cultivars, “Cuiguan” and “Cuiyu”. Overall, expression of PpySDHs peaked twice, first at the fruitlet stage and again at or near harvest. The transcript abundance of PpySDHs was higher in “Cuiguan” than in “Cuiyu”, accompanied by a higher content of sugars and higher ratio of fructose to sorbitol maintained in the former cultivar at harvest. In conclusion, it was suggested that multiple members of the SDH gene family are possibly involved in sand Pear fruit development and sugar accumulation and may affect both the sugar amount and sugar composition.
C L Blomquist - One of the best experts on this subject based on the ideXlab platform.
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identification of phytoplasma taxa and insect vectors of peach yellow leaf roll disease in california
Plant Disease, 2002Co-Authors: C L Blomquist, Bruce C KirkpatrickAbstract:ABSTRACT Two peach diseases in California, western X-disease (WX) and peach yellow leaf roll (PYLR) are caused by two genetically distinct phytoplasmas. Based on symptoms alone, WX and PYLR cannot be reliably distinguished. The objectives of this study were to determine which phytoplasma was causing PYLR disease in peach orchards planted near Pear orchards and which phytoplasmas were present in potential insect vectors captured in both peach and Pear orchards. Leafhoppers and Pear psylla were collected from yellow sticky traps placed in three Pear orchards and four peach orchards located in the Sacramento Valley from 1994 to 1996. DNA was extracted from potential vectors and suspect diseased trees, and analyzed for the presence of the WX and PYLR phytoplasmas using strain-specific DNA hybridization or polymerase chain reaction (PCR) assays. The most abundant phytoplasma detected in diseased peach trees was the PYLR phytoplasma. In 1994 and 1995, 10 to 25% of groups of Pear psylla tested positive for the P...
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identification of phytoplasma taxa and insect vectors of peach yellow leaf roll disease in california
Plant Disease, 2002Co-Authors: C L Blomquist, Bruce C KirkpatrickAbstract:Two peach diseases in California, western X-disease (WX) and peach yellow leaf roll (PYLR) are caused by two genetically distinct phytoplasmas. Based on symptoms alone, WX and PYLR cannot be reliably distinguished. The objectives of this study were to determine which phytoplasma was causing PYLR disease in peach orchards planted near Pear orchards and which phytoplasmas were present in potential insect vectors captured in both peach and Pear orchards. Leafhoppers and Pear psylla were collected from yellow sticky traps placed in three Pear orchards and four peach orchards located in the Sacramento Valley from 1994 to 1996. DNA was extracted from potential vectors and suspect diseased trees, and analyzed for the presence of the WX and PYLR phytoplasmas using strain-specific DNA hybridization or polymerase chain reaction (PCR) assays. The most abundant phytoplasma detected in diseased peach trees was the PYLR phytoplasma. In 1994 and 1995, 10 to 25% of groups of Pear psylla tested positive for the PYLR phytoplasma, while no psylla groups tested positive for the WX phytoplasma. Only one captured leafhopper tested positive for the WX phytoplasma. These results indicate the Pear psylla is the primary vector of PYLR in northern California.
Takayuki Kojima - One of the best experts on this subject based on the ideXlab platform.
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Near-Infrared Monitoring of the Growth Period of Japanese Pear Fruit Based on Constituent Sugar Concentrations
Journal of Agricultural and Food Chemistry, 1996Co-Authors: Munehiro Tanaka, Takayuki KojimaAbstract:To develop nondestructive monitoring techniques in plant science, near-infrared spectroscopy (near-IRS) was applied to estimate the growth stages of the fruit of the Japanese Pear tree based on the constituent sugar concentrations in the juice. Regular differences were observed among the spectra of juice from Pears of different maturity, and the absorption bands of these spectra were assigned to carbohydrate. The multiple linear regression calibration equations of each constituent sugar (namely, sucrose, glucose, fructose, and sorbitol) used three to seven wavelengths as independent variables. The correlation coefficients of each sugar concentration were 0.97, 0.90, 0.99, and 0.98, with standard error of prediction values of 0.17, 0.15, 0.21, and 0.40 respectively. Results of measurement of each constituent sugar concentration in Japanese Pear juice by near-IRS indicated that the sugar components changed with the growth period of fruits. Therefore, near-IRS can be used to effectively evaluate the growth s...
