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Kwok-yung Yuen - One of the best experts on this subject based on the ideXlab platform.

  • Draft Genome Sequence of Penicillium marneffei Strain PM1
    Eukaryotic cell, 2011
    Co-Authors: Patrick C. Y. Woo, Susanna K. P. Lau, Bin Liu, James J. Cai, Ken T. K. Chong, Herman Tse, Richard Y. T. Kao, Che-man Chan, Wang-ngai Chow, Kwok-yung Yuen
    Abstract:

    ABSTRACT Penicillium marneffei is the most important thermal dimorphic, pathogenic fungus endemic in China and Southeast Asia and is particularly important in HIV-positive patients. We report the 28,887,485-bp draft genome sequence of P. marneffei, which contains its complete mitochondrial genome, sexual cycle genes, a high diversity of Mp1p homologues, and polyketide synthase genes.

  • Asymptomatic Penicillium marneffei fungemia in an HIV-infected patient.
    International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases, 2006
    Co-Authors: Teresa K. F. Wang, Kwok-yung Yuen, Samson S. Y. Wong
    Abstract:

    Penicillium marneffei is an AIDS-defining endemic mycosis in Southeast Asia. Patients usually present with fever weight loss mucocutaneous lesions lymphadenopathy hepatomegaly or pulmonary infiltrates. We hereby report the first case of asymptomatic P. marneffei fungemia in an HIV-infected patient. (excerpt)

  • Penicillium marneffei fungaemia in an allogeneic bone marrow transplant recipient
    Bone Marrow Transplantation, 2005
    Co-Authors: Patrick C. Y. Woo, Samson S. Y. Wong, Susanna K. P. Lau, Ken T. K. Chong, Candy Choiyi Lau, Waiting Hui, Kwok-yung Yuen
    Abstract:

    Penicillium marneffei is the most important thermal dimorphic fungus causing respiratory, skin and systemic mycosis in Southeast Asia.1, 2, 3 About 8% of AIDS patients in Hong Kong are infected with P. marneffei. Clinically, penicilliosis manifests as a systemic febrile illness, which results from intracellular infection of the reticuloendothelial cells by the yeast phase of the fungus. Besides HIV-positive patients, P. marneffei infections have been reported in other immunocompromised patients, such as renal transplant recipients, patients with systemic lupus erythematosus and patients who are receiving corticosteroid therapy.4, 5 Here, we describe the first case of P. marneffei infection in a bone marrow transplant recipient.

  • Preparation and identification of monoclonal antibodies against Penicillium marneffei
    Di 1 jun yi da xue xue bao = Academic journal of the first medical college of PLA, 2003
    Co-Authors: Li-wen Qiu, Dong-xian Peng, Juan Jia, Xiao-yan Che, Kwok-yung Yuen
    Abstract:

    Objective To prepare and identify monoclonal antibodies (mAbs) against Penicillium marneffei. Methods Recombinant mannoprotein1 (MP1) of Penicillium marneffei was used to immunize BALB/c mice, and anti-MP1 mAbs were obtained by means of hybridoma. Screening and identification of the mAbs were subsequently performed with indirect enzyme-linked immunosorbent assay and Western blotting, respectively. Results Four hybridomas producing antibodies against Penicillium marneffei were obtained, and the IgG isotypes of the 4 mAbs were identified as IgG1 with affinity constants (K) of 8.2x10(-9), 4.7x10(-9), 6.5x10(-9) and 2.7x10(-9), respectively. Western blotting demonstrated specific recognition of Aspergillus fumigatus MP1 by the obtained mAbs. Conclusion The 4 hybridomas producing anti-MP1 mAbs with high specificity and affinity can be of significant value in the diagnosis of Penicilliosis marneffei infections.

  • Candida tropicalis and Penicillium marneffei Mixed Fungaemia in a Patient with Waldenström's Macroglobulinaemia
    European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology, 2001
    Co-Authors: Samson S. Y. Wong, Patrick C. Y. Woo, Kwok-yung Yuen
    Abstract:

    A patient with Waldenstrom's macroglobulinaemia who had previously been treated with cladribine presented with septic arthritis caused by Escherichia coli. The patient's condition subsequently deteriorated, and he succumbed to pneumonia and mixed fungaemia due to Candida tropicalis and Penicillium marneffei. Profound lymphopenia coexisted with fungaemia. This is the first reported case of mixed Penicillium marneffei and Candida fungaemia and penicilliosis marneffei in a patient with Waldenstrom's macroglobulinaemia. Penicilliosis marneffei should be considered as a potential complication in patients with markedly impaired cell-mediated immunity who have travelled to or resided in endemic areas. Patients who have undergone therapy with purine nucleoside analogues such as fludarabine and cladribine are also at risk.

Patrick C. Y. Woo - One of the best experts on this subject based on the ideXlab platform.

  • Draft Genome Sequence of Penicillium marneffei Strain PM1
    Eukaryotic cell, 2011
    Co-Authors: Patrick C. Y. Woo, Susanna K. P. Lau, Bin Liu, James J. Cai, Ken T. K. Chong, Herman Tse, Richard Y. T. Kao, Che-man Chan, Wang-ngai Chow, Kwok-yung Yuen
    Abstract:

    ABSTRACT Penicillium marneffei is the most important thermal dimorphic, pathogenic fungus endemic in China and Southeast Asia and is particularly important in HIV-positive patients. We report the 28,887,485-bp draft genome sequence of P. marneffei, which contains its complete mitochondrial genome, sexual cycle genes, a high diversity of Mp1p homologues, and polyketide synthase genes.

  • Penicillium marneffei fungaemia in an allogeneic bone marrow transplant recipient
    Bone Marrow Transplantation, 2005
    Co-Authors: Patrick C. Y. Woo, Samson S. Y. Wong, Susanna K. P. Lau, Ken T. K. Chong, Candy Choiyi Lau, Waiting Hui, Kwok-yung Yuen
    Abstract:

    Penicillium marneffei is the most important thermal dimorphic fungus causing respiratory, skin and systemic mycosis in Southeast Asia.1, 2, 3 About 8% of AIDS patients in Hong Kong are infected with P. marneffei. Clinically, penicilliosis manifests as a systemic febrile illness, which results from intracellular infection of the reticuloendothelial cells by the yeast phase of the fungus. Besides HIV-positive patients, P. marneffei infections have been reported in other immunocompromised patients, such as renal transplant recipients, patients with systemic lupus erythematosus and patients who are receiving corticosteroid therapy.4, 5 Here, we describe the first case of P. marneffei infection in a bone marrow transplant recipient.

  • Candida tropicalis and Penicillium marneffei Mixed Fungaemia in a Patient with Waldenström's Macroglobulinaemia
    European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology, 2001
    Co-Authors: Samson S. Y. Wong, Patrick C. Y. Woo, Kwok-yung Yuen
    Abstract:

    A patient with Waldenstrom's macroglobulinaemia who had previously been treated with cladribine presented with septic arthritis caused by Escherichia coli. The patient's condition subsequently deteriorated, and he succumbed to pneumonia and mixed fungaemia due to Candida tropicalis and Penicillium marneffei. Profound lymphopenia coexisted with fungaemia. This is the first reported case of mixed Penicillium marneffei and Candida fungaemia and penicilliosis marneffei in a patient with Waldenstrom's macroglobulinaemia. Penicilliosis marneffei should be considered as a potential complication in patients with markedly impaired cell-mediated immunity who have travelled to or resided in endemic areas. Patients who have undergone therapy with purine nucleoside analogues such as fludarabine and cladribine are also at risk.

Nongnuch Vanittanakom - One of the best experts on this subject based on the ideXlab platform.

  • Insights into the pathogenicity of Penicillium marneffei
    Future microbiology, 2008
    Co-Authors: Chester R. Cooper, Nongnuch Vanittanakom
    Abstract:

    Penicillium marneffei is a significant pathogen of AIDS patients in Southeast Asia. This fungus is unique in that it is the only dimorphic member of the genus. Pathogenesis of P. marneffei requires the saprobic mold form to undergo a morphological change upon tissue invasion. The in vivo form of this fungus reproduces as a fission yeast that capably evades the host immune system. The processes that control these morphological changes, better termed as phase transition, can be replicated in vitro by incubation of the mold form at 37°C. The unidentified molecular mechanisms regulating phase transition in this fungus are now being uncovered using modern methodologies and novel strategies. A better comprehension of these underlying regulatory pathways will provide insight into eukaryotic cellular development as well as the potential factors responsible for infections caused by P. marneffei and other fungi. Such knowledge may lead to better chemotherapeutic interventions of fungal diseases.

  • a highly discriminatory multilocus microsatellite typing mlmt system for Penicillium marneffei
    Molecular Ecology Notes, 2004
    Co-Authors: Matthew C Fisher, Sybren De Hoog, Nongnuch Vanittanakom
    Abstract:

    For eukaryotic pathogens that are depauperate in genetic variation, multilocus microsatellite typing (MLMT) offers an accurate and reproducible method of characterizing genetic diversity; herein we describe the development of an MLMT system for the emerging pathogenic fungus Penicillium marneffei based on 23 microsatellite loci. Screening isolates held within the Centraalbureau voor Schimmelcultures culture collection demonstrate high levels of genetic diversity and 100% reproducibility. This MLMT system provides a powerful epidemiological tool to analyse the underlying parameters that are responsible for the emergence of P. marneffei in human HIV-positive populations.

  • Sialic Acid-Dependent Recognition of Laminin by Penicillium marneffei Conidia
    Infection and immunity, 1998
    Co-Authors: Andrew Hamilton, Nongnuch Vanittanakom, Sirida Youngchim, L. Jeavons, Roderick J. Hay
    Abstract:

    Immunofluorescence microscopy demonstrated that laminin bound to the surface of Penicillium marneffei conidia. Attachment of P. marneffei conidia in an adherence assay was inhibited by soluble laminin and anti-laminin antibody. N-Acetylneuraminic acid abolished adherence, indicating an interaction mediated by a sialic acid-specific lectin.

  • Restriction endonuclease analysis of Penicillium marneffei.
    Journal of clinical microbiology, 1996
    Co-Authors: Nongnuch Vanittanakom, Chester R. Cooper, Suwat Chariyalertsak, Sirida Youngchim, Kenrad E. Nelson, Thira Sirisanthana
    Abstract:

    Forty-six isolates of Penicillium marneffei were differentiated into two DNA types on the basis of their restriction fragment length polymorphisms. Of the 22 human isolates of P. marneffei, 16 (72.7%) were type I and 6 (27.3%) were type II. Of the 23 bamboo rat isolates, 20 from Rhizomys sumatrensis were type I and 3 from Cannomys badius were type II. The soil isolate was type II. These data represent the first molecular epidemiological study of this important emerging fungal pathogen.

Roderick J. Hay - One of the best experts on this subject based on the ideXlab platform.

Susanna K. P. Lau - One of the best experts on this subject based on the ideXlab platform.

  • Draft Genome Sequence of Penicillium marneffei Strain PM1
    Eukaryotic cell, 2011
    Co-Authors: Patrick C. Y. Woo, Susanna K. P. Lau, Bin Liu, James J. Cai, Ken T. K. Chong, Herman Tse, Richard Y. T. Kao, Che-man Chan, Wang-ngai Chow, Kwok-yung Yuen
    Abstract:

    ABSTRACT Penicillium marneffei is the most important thermal dimorphic, pathogenic fungus endemic in China and Southeast Asia and is particularly important in HIV-positive patients. We report the 28,887,485-bp draft genome sequence of P. marneffei, which contains its complete mitochondrial genome, sexual cycle genes, a high diversity of Mp1p homologues, and polyketide synthase genes.

  • Penicillium marneffei fungaemia in an allogeneic bone marrow transplant recipient
    Bone Marrow Transplantation, 2005
    Co-Authors: Patrick C. Y. Woo, Samson S. Y. Wong, Susanna K. P. Lau, Ken T. K. Chong, Candy Choiyi Lau, Waiting Hui, Kwok-yung Yuen
    Abstract:

    Penicillium marneffei is the most important thermal dimorphic fungus causing respiratory, skin and systemic mycosis in Southeast Asia.1, 2, 3 About 8% of AIDS patients in Hong Kong are infected with P. marneffei. Clinically, penicilliosis manifests as a systemic febrile illness, which results from intracellular infection of the reticuloendothelial cells by the yeast phase of the fungus. Besides HIV-positive patients, P. marneffei infections have been reported in other immunocompromised patients, such as renal transplant recipients, patients with systemic lupus erythematosus and patients who are receiving corticosteroid therapy.4, 5 Here, we describe the first case of P. marneffei infection in a bone marrow transplant recipient.