The Experts below are selected from a list of 255 Experts worldwide ranked by ideXlab platform
Dennis J. Pillion - One of the best experts on this subject based on the ideXlab platform.
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sucrose cocoate a component of cosmetic preparations enhances nasal and ocular Peptide Absorption
International Journal of Pharmaceutics, 2003Co-Authors: Fakhrul Ahsan, John J. Arnold, Elias Meezan, Dennis J. PillionAbstract:Sucrose cocoate (SL-40), an emulsifier employed in emollient, skin-moisturizing cosmetic formulations, contains a mixture of sucrose esters of coconut fatty acids in aqueous ethanol solution. In order to determine its potential utility in enhancing nasal and ocular drug delivery, Absorption studies were performed in anesthetized Sprague-Dawley male rats with calcitonin and insulin, two distinct therapeutic Peptides. Administration of a nasal insulin formulation containing 0.5% sucrose cocoate caused a rapid and significant increase in plasma insulin levels, with a concomitant decrease in blood glucose levels. When insulin was administered ocularly in the presence of 0.5% sucrose cocoate, a smaller increase in plasma insulin levels, and a decrease in blood glucose levels, were observed. Administration of a nasal calcitonin formulation containing 0.5% sucrose cocoate caused a rapid increase in plasma calcitonin levels and a concomitant decrease in plasma calcium levels. Mass spectrometric analyses were used to characterize the nature of the sucrose fatty acid esters in the mixture. The most abundant sucrose ester in sucrose cocoate was sucrose monododecanoate, with smaller amounts of sucrose monodecanoate and sucrose monotetradecanoate. In vivo experiments confirmed that this ester was an effective enhancer of nasal Peptide drug Absorption.
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Sucrose cocoate, a component of cosmetic preparations, enhances nasal and ocular Peptide Absorption
International Journal of Pharmaceutics, 2003Co-Authors: Fakhrul Ahsan, John J. Arnold, Elias Meezan, Dennis J. PillionAbstract:Sucrose cocoate (SL-40), an emulsifier employed in emollient, skin-moisturizing cosmetic formulations, contains a mixture of sucrose esters of coconut fatty acids in aqueous ethanol solution. In order to determine its potential utility in enhancing nasal and ocular drug delivery, Absorption studies were performed in anesthetized Sprague-Dawley male rats with calcitonin and insulin, two distinct therapeutic Peptides. Administration of a nasal insulin formulation containing 0.5% sucrose cocoate caused a rapid and significant increase in plasma insulin levels, with a concomitant decrease in blood glucose levels. When insulin was administered ocularly in the presence of 0.5% sucrose cocoate, a smaller increase in plasma insulin levels, and a decrease in blood glucose levels, were observed. Administration of a nasal calcitonin formulation containing 0.5% sucrose cocoate caused a rapid increase in plasma calcitonin levels and a concomitant decrease in plasma calcium levels. Mass spectrometric analyses were used to characterize the nature of the sucrose fatty acid esters in the mixture. The most abundant sucrose ester in sucrose cocoate was sucrose monododecanoate, with smaller amounts of sucrose monodecanoate and sucrose monotetradecanoate. In vivo experiments confirmed that this ester was an effective enhancer of nasal Peptide drug Absorption. © 2002 Published by Elsevier Science B.V.
Michael G Sarr - One of the best experts on this subject based on the ideXlab platform.
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Peptide Absorption after massive proximal small bowel resection mechanisms of ileal adaptation
Journal of Gastrointestinal Surgery, 2011Co-Authors: Hisham G Qandeel, Fernando Alonso, David J Hernandez, Srivats Madhavan, Judith A Duenes, Ye Zheng, Michael G SarrAbstract:Background Protein Absorption occurs as di- and tri-Peptides via H+/Peptide co-transporter-1 (PepT1).
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Intestinal Adaptation for OligoPeptide Absorption via PepT1 After Massive (70%) Mid-Small Bowel Resection
Journal of Gastrointestinal Surgery, 2011Co-Authors: Srivats Madhavan, Judith A Duenes, Ye Zheng, Jeffrey S. Scow, Rizwan M. Chaudhry, Munenori Nagao, Michael G SarrAbstract:Introduction Proteins are absorbed primarily as short Peptides via Peptide transporter 1 (PepT1). Hypothesis Intestinal adaptation for Peptide Absorption after massive mid-small intestinal resection occurs by increased expression of PepT1 in the remnant small intestine and colon. Methods Peptide uptake was measured in duodenum, jejunum, ileum, and colon using glycyl-sarcosine 1 week ( n = 9) and 4 weeks ( n = 11) after 70% mid-small bowel resection and in corresponding segments from unoperated rats ( n = 12) and after transection and reanastomosis of jejunum and ileum ( n = 8). Expression of PepT1 (mRNA, protein) and villus height were measured. Results Intestinal transection/reanastomosis did not alter gene expression. Compared to non-operated controls, 70% mid-small bowel resection increased jejunal Peptide uptake ( p
Nikhil J. Kavimandan - One of the best experts on this subject based on the ideXlab platform.
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nanoscale analysis of protein and Peptide Absorption insulin Absorption using complexation and ph sensitive hydrogels as delivery vehicles
European Journal of Pharmaceutical Sciences, 2006Co-Authors: Nicholas A. Peppas, Nikhil J. KavimandanAbstract:Abstract Recent advances in the discovery and delivery of drugs to cure chronic diseases are achieved by combination of intelligent material design with advances in nanotechnology. Since many drugs act as protagonists or antagonists to different chemicals in the body, a delivery system that can respond to the concentrations of certain molecules in the body is invaluable. For this purpose, intelligent therapeutics or “smart drug delivery” calls for the design of the newest generation of sensitive materials based on molecular recognition. Biomimetic polymeric networks can be prepared by designing interactions between the building blocks of biocompatible networks and the desired specific ligands and by stabilizing these interactions by a three-dimensional structure. These structures are at the same time flexible enough to allow for diffusion of solvent and ligand into and out of the networks. Synthetic networks that can be designed to recognize and bind biologically significant molecules are of great importance and influence a number of emerging technologies. These synthetic materials can be used as unique systems or incorporated into existing drug delivery technologies that can aid in the removal or delivery of biomolecules and restore the natural profiles of compounds in the body.
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Nanoscale analysis of protein and Peptide Absorption: Insulin Absorption using complexation and pH-sensitive hydrogels as delivery vehicles
European Journal of Pharmaceutical Sciences, 2006Co-Authors: Nicholas A. Peppas, Nikhil J. KavimandanAbstract:Recent advances in the discovery and delivery of drugs to cure chronic diseases are achieved by combination of intelligent material design with advances in nanotechnology. Since many drugs act as protagonists or antagonists to different chemicals in the body, a delivery system that can respond to the concentrations of certain molecules in the body is invaluable. For this purpose, intelligent therapeutics or "smart drug delivery" calls for the design of the newest generation of sensitive materials based on molecular recognition. Biomimetic polymeric networks can be prepared by designing interactions between the building blocks of biocompatible networks and the desired specific ligands and by stabilizing these interactions by a three-dimensional structure. These structures are at the same time flexible enough to allow for diffusion of solvent and ligand into and out of the networks. Synthetic networks that can be designed to recognize and bind biologically significant molecules are of great importance and influence a number of emerging technologies. These synthetic materials can be used as unique systems or incorporated into existing drug delivery technologies that can aid in the removal or delivery of biomolecules and restore the natural profiles of compounds in the body. ?? 2006 Elsevier B.V. All rights reserved.
Xinbei Huang - One of the best experts on this subject based on the ideXlab platform.
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Mechanism of Peptide Absorption in the isolated forestomach epithelial cells of dairy cows
Journal of the Science of Food and Agriculture, 2018Co-Authors: Qingbiao Xu, Feng-qi Zhao, Yueming Wu, Xinbei HuangAbstract:Peptide Absorption from the forestomach plays a vital role in protein nutrition of dairy cows. This study was conducted to investigate the mechanism of diPeptide Absorption in the forestomach of dairy cows using isolated omasal epithelial cells (OECs) and ruminal epithelial cells (RECs). RESULTS Compared with RECs, the OECs formed a less tight monolayer, but had greater ability to transport glycylsarcosine (Gly-Sar) (P
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establishment and characterization of an omasal epithelial cell model derived from dairy calves for the study of small Peptide Absorption
PLOS ONE, 2014Co-Authors: Qingbiao Xu, Yueming Wu, Xinbei HuangAbstract:The objective of this study was to establish a primary culture of omasal epithelial cells (OECs) derived from dairy calves and to characterize its function in small Peptide Absorption. Bovine omasal tissues were obtained from newborn Chinese Holstein calves and digested with a 2.5% trypsin solution to obtain OECs. The isolated cells were later cultured in DMEM containing 10% fetal bovine serum, 5 μg/ml insulin, 10 ng/ml epidermal growth factor, 100 U/ml penicillin, 100 μg/ml streptomycin, 50 μg/ml gentamycin and 2.5 μg/ml amphotericin B. Hematoxylin and eosin staining of omasal tissue after digestion indicated that the cultured cells originated from the epithelial strata. Pure epithelial cells displayed an epithelial cell-like morphology, similar to cobblestone, with few visible fibroblasts and were cytokeratin 18-positive according to immunocytochemical analyses. The OECs were morphologically characterized with desmosomes, tight junctions and microvilli. These cells exhibited normal growth properties, as assessed using a cell growth curve, and were stably cultured for 10 passages. The OECs expressed the Peptide transporter 1 (PEPT1) mRNA and absorbed intact glycylsarcosine (Gly-Sar). The uptake of Gly-Sar by OECs was pH-dependent with an optimal pH of 5.5–6.5. Furthermore, the uptake of Gly-Sar was also time-dependent, concentration-dependent and temperature-dependent. Moreover, PEPT1 was saturated with Gly-Sar at a concentration of 2.5 mM. The uptake via PEPT1 was higher compared with that via passive route at low substrate concentrations (<1.5 mM). This result suggested that PEPT1 contributed more to total small Peptide Absorption at low concentrations. In addition, this uptake could be competitively inhibited by methionine-glycine. Taken together, these data suggested that PEPT1 contributes to small Peptide Absorption in OECs. Thus, OECs may serve as a useful culture model for the study of the Absorption of small Peptides in bovine omasum.
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Establishment and characterization of an omasal epithelial cell model derived from dairy calves for the study of small Peptide Absorption.
PLOS ONE, 2014Co-Authors: Qingbiao Xu, Yueming Wu, Xinbei HuangAbstract:The objective of this study was to establish a primary culture of omasal epithelial cells (OECs) derived from dairy calves and to characterize its function in small Peptide Absorption. Bovine omasal tissues were obtained from newborn Chinese Holstein calves and digested with a 2.5% trypsin solution to obtain OECs. The isolated cells were later cultured in DMEM containing 10% fetal bovine serum, 5 μg/ml insulin, 10 ng/ml epidermal growth factor, 100 U/ml penicillin, 100 μg/ml streptomycin, 50 μg/ml gentamycin and 2.5 μg/ml amphotericin B. Hematoxylin and eosin staining of omasal tissue after digestion indicated that the cultured cells originated from the epithelial strata. Pure epithelial cells displayed an epithelial cell-like morphology, similar to cobblestone, with few visible fibroblasts and were cytokeratin 18-positive according to immunocytochemical analyses. The OECs were morphologically characterized with desmosomes, tight junctions and microvilli. These cells exhibited normal growth properties, as assessed using a cell growth curve, and were stably cultured for 10 passages. The OECs expressed the Peptide transporter 1 (PEPT1) mRNA and absorbed intact glycylsarcosine (Gly-Sar). The uptake of Gly-Sar by OECs was pH-dependent with an optimal pH of 5.5–6.5. Furthermore, the uptake of Gly-Sar was also time-dependent, concentration-dependent and temperature-dependent. Moreover, PEPT1 was saturated with Gly-Sar at a concentration of 2.5 mM. The uptake via PEPT1 was higher compared with that via passive route at low substrate concentrations (
Fakhrul Ahsan - One of the best experts on this subject based on the ideXlab platform.
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sucrose cocoate a component of cosmetic preparations enhances nasal and ocular Peptide Absorption
International Journal of Pharmaceutics, 2003Co-Authors: Fakhrul Ahsan, John J. Arnold, Elias Meezan, Dennis J. PillionAbstract:Sucrose cocoate (SL-40), an emulsifier employed in emollient, skin-moisturizing cosmetic formulations, contains a mixture of sucrose esters of coconut fatty acids in aqueous ethanol solution. In order to determine its potential utility in enhancing nasal and ocular drug delivery, Absorption studies were performed in anesthetized Sprague-Dawley male rats with calcitonin and insulin, two distinct therapeutic Peptides. Administration of a nasal insulin formulation containing 0.5% sucrose cocoate caused a rapid and significant increase in plasma insulin levels, with a concomitant decrease in blood glucose levels. When insulin was administered ocularly in the presence of 0.5% sucrose cocoate, a smaller increase in plasma insulin levels, and a decrease in blood glucose levels, were observed. Administration of a nasal calcitonin formulation containing 0.5% sucrose cocoate caused a rapid increase in plasma calcitonin levels and a concomitant decrease in plasma calcium levels. Mass spectrometric analyses were used to characterize the nature of the sucrose fatty acid esters in the mixture. The most abundant sucrose ester in sucrose cocoate was sucrose monododecanoate, with smaller amounts of sucrose monodecanoate and sucrose monotetradecanoate. In vivo experiments confirmed that this ester was an effective enhancer of nasal Peptide drug Absorption.
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Sucrose cocoate, a component of cosmetic preparations, enhances nasal and ocular Peptide Absorption
International Journal of Pharmaceutics, 2003Co-Authors: Fakhrul Ahsan, John J. Arnold, Elias Meezan, Dennis J. PillionAbstract:Sucrose cocoate (SL-40), an emulsifier employed in emollient, skin-moisturizing cosmetic formulations, contains a mixture of sucrose esters of coconut fatty acids in aqueous ethanol solution. In order to determine its potential utility in enhancing nasal and ocular drug delivery, Absorption studies were performed in anesthetized Sprague-Dawley male rats with calcitonin and insulin, two distinct therapeutic Peptides. Administration of a nasal insulin formulation containing 0.5% sucrose cocoate caused a rapid and significant increase in plasma insulin levels, with a concomitant decrease in blood glucose levels. When insulin was administered ocularly in the presence of 0.5% sucrose cocoate, a smaller increase in plasma insulin levels, and a decrease in blood glucose levels, were observed. Administration of a nasal calcitonin formulation containing 0.5% sucrose cocoate caused a rapid increase in plasma calcitonin levels and a concomitant decrease in plasma calcium levels. Mass spectrometric analyses were used to characterize the nature of the sucrose fatty acid esters in the mixture. The most abundant sucrose ester in sucrose cocoate was sucrose monododecanoate, with smaller amounts of sucrose monodecanoate and sucrose monotetradecanoate. In vivo experiments confirmed that this ester was an effective enhancer of nasal Peptide drug Absorption. © 2002 Published by Elsevier Science B.V.