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Rengarajulu Puvanakrishnan - One of the best experts on this subject based on the ideXlab platform.
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A novel Peptide Derivative exhibits anti inflammatory and antioxidant activity in adjuvant induced arthritis in rats.
Molecular and cellular biochemistry, 2020Co-Authors: Dilly Ashok Kumar, Panchatcharam Manikandan, Miriyala Sumitra, Chandrasekar Gayathri, Natarajan Arutselvan, Kalidindi V S Narayana Raju, Rengarajulu PuvanakrishnanAbstract:A tetraPeptide Derivative PEP1261 [Boc-Lys-(Boc)-Arg-Asp-Ser-(tBu)-OtBu], corresponding to residues 39-42 of human lactoferrin, was tested for its antiinflammatory action in adjuvant induced arthritis in rats. Administration of heat killed Mycobacterium tuberculosis (500 microg/0.1 ml of paraffin oil) intradermally into the foot pad of right hind paw resulted in an increased paw volume and an increase in the levels of reactive oxygen species and beta-glucuronidase as well as a decrease in the antioxidants levels. PEP1261, at an effective dose of 10 mg/kg body wt., exhibited a significant antiarthritic activity as evidenced by lowering of paw volume and inhibited the free radicals toxicity by increasing the antioxidants levels. This Peptide Derivative was also shown to have a membrane stabilizing action by significantly decreasing the total and free activity of beta-glucuronidase and inhibiting the rate of release of the enzyme from lysosomal rich fraction. Histopathological studies confirmed the above results by showing a decrease in mononuclear cell infiltration, hypertrophy, hyperplasia and pannus formation after PEP1261 treatment in arthritic rats.
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a novel Peptide Derivative exhibits anti inflammatory and antioxidant activity in adjuvant induced arthritis in rats
Molecular and Cellular Biochemistry, 2002Co-Authors: Dilly Ashok Kumar, Panchatcharam Manikandan, Miriyala Sumitra, Chandrasekar Gayathri, Natarajan Arutselvan, Kalidindi Narayana V S Raju, Rengarajulu PuvanakrishnanAbstract:A tetraPeptide Derivative PEP1261 {Boc-Lys-(Boc)-Arg-Asp-Ser-(tBu)-OtBu}, corresponding to residues 39–42 of human lactoferrin, was tested for its antiinflammatory action in adjuvant induced arthritis in rats. Administration of heat killed Mycobacterium tuberculosis (500 μg/0.1 ml of paraffin oil) intradermally into the foot pad of right hind paw resulted in an increased paw volume and an increase in the levels of reactive oxygen species and β-glucuronidase as well as a decrease in the antioxidants levels. PEP1261, at an effective dose of 10 mg/kg body wt., exhibited a significant antiarthritic activity as evidenced by lowering of paw volume and inhibited the free radicals toxicity by increasing the antioxidants levels. This Peptide Derivative was also shown to have a membrane stabilizing action by significantly decreasing the total and free activity of β-glucuronidase and inhibiting the rate of release of the enzyme from lysosomal rich fraction. Histopathological studies confirmed the above results by showing a decrease in mononuclear cell infiltration, hypertrophy, hyperplasia and pannus formation after PEP1261 treatment in arthritic rats.
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A novel Peptide Derivative exhibits antiinflammatory and antioxidant activity in adjuvant induced arthritis in rats
Molecular and Cellular Biochemistry, 2002Co-Authors: Dilly Ashok Kumar, Panchatcharam Manikandan, Miriyala Sumitra, Kalidindi V.s. Narayana Raju, Chandrasekar Gayathri, Natarajan Arutselvan, Rengarajulu PuvanakrishnanAbstract:A tetraPeptide Derivative PEP1261 {Boc-Lys-(Boc)-Arg-Asp-Ser-(tBu)-OtBu}, corresponding to residues 39–42 of human lactoferrin, was tested for its antiinflammatory action in adjuvant induced arthritis in rats. Administration of heat killed Mycobacterium tuberculosis (500 μg/0.1 ml of paraffin oil) intradermally into the foot pad of right hind paw resulted in an increased paw volume and an increase in the levels of reactive oxygen species and β-glucuronidase as well as a decrease in the antioxidants levels. PEP1261, at an effective dose of 10 mg/kg body wt., exhibited a significant antiarthritic activity as evidenced by lowering of paw volume and inhibited the free radicals toxicity by increasing the antioxidants levels. This Peptide Derivative was also shown to have a membrane stabilizing action by significantly decreasing the total and free activity of β-glucuronidase and inhibiting the rate of release of the enzyme from lysosomal rich fraction. Histopathological studies confirmed the above results by showing a decrease in mononuclear cell infiltration, hypertrophy, hyperplasia and pannus formation after PEP1261 treatment in arthritic rats.
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A novel surface-active Peptide Derivative exhibits in vitro inhibition of platelet aggregation
Peptides, 1998Co-Authors: C.v. Ramesh, Rajadas Jayakumar, Rengarajulu PuvanakrishnanAbstract:Abstract A tetraPeptide corresponding to a region of the N-terminal portion of lactotransferrin with hydrophobic alkyl groups at the terminal ends was synthesized and its physicochemical properties as well as its effect on thrombin-stimulated platelet aggregation were examined. The tetraPeptide Derivative, in the aggregated state, produced inhibitory effect on platelet aggregation. The concentration dependent activity of the Peptide was analyzed in the light of micelle formation, with the micellar aggregate comprising four tetraPeptide units. The unique action of this Peptide Derivative on the inhibition of platelet aggregation might be useful in the development of potent antithrombotic drugs.
Dilly Ashok Kumar - One of the best experts on this subject based on the ideXlab platform.
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A novel Peptide Derivative exhibits anti inflammatory and antioxidant activity in adjuvant induced arthritis in rats.
Molecular and cellular biochemistry, 2020Co-Authors: Dilly Ashok Kumar, Panchatcharam Manikandan, Miriyala Sumitra, Chandrasekar Gayathri, Natarajan Arutselvan, Kalidindi V S Narayana Raju, Rengarajulu PuvanakrishnanAbstract:A tetraPeptide Derivative PEP1261 [Boc-Lys-(Boc)-Arg-Asp-Ser-(tBu)-OtBu], corresponding to residues 39-42 of human lactoferrin, was tested for its antiinflammatory action in adjuvant induced arthritis in rats. Administration of heat killed Mycobacterium tuberculosis (500 microg/0.1 ml of paraffin oil) intradermally into the foot pad of right hind paw resulted in an increased paw volume and an increase in the levels of reactive oxygen species and beta-glucuronidase as well as a decrease in the antioxidants levels. PEP1261, at an effective dose of 10 mg/kg body wt., exhibited a significant antiarthritic activity as evidenced by lowering of paw volume and inhibited the free radicals toxicity by increasing the antioxidants levels. This Peptide Derivative was also shown to have a membrane stabilizing action by significantly decreasing the total and free activity of beta-glucuronidase and inhibiting the rate of release of the enzyme from lysosomal rich fraction. Histopathological studies confirmed the above results by showing a decrease in mononuclear cell infiltration, hypertrophy, hyperplasia and pannus formation after PEP1261 treatment in arthritic rats.
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A novel Peptide Derivative exhibits antiinflammatory and antioxidant activity in adjuvant induced arthritis in rats
Molecular and Cellular Biochemistry, 2002Co-Authors: Dilly Ashok Kumar, Panchatcharam Manikandan, Miriyala Sumitra, Kalidindi V.s. Narayana Raju, Chandrasekar Gayathri, Natarajan Arutselvan, Rengarajulu PuvanakrishnanAbstract:A tetraPeptide Derivative PEP1261 {Boc-Lys-(Boc)-Arg-Asp-Ser-(tBu)-OtBu}, corresponding to residues 39–42 of human lactoferrin, was tested for its antiinflammatory action in adjuvant induced arthritis in rats. Administration of heat killed Mycobacterium tuberculosis (500 μg/0.1 ml of paraffin oil) intradermally into the foot pad of right hind paw resulted in an increased paw volume and an increase in the levels of reactive oxygen species and β-glucuronidase as well as a decrease in the antioxidants levels. PEP1261, at an effective dose of 10 mg/kg body wt., exhibited a significant antiarthritic activity as evidenced by lowering of paw volume and inhibited the free radicals toxicity by increasing the antioxidants levels. This Peptide Derivative was also shown to have a membrane stabilizing action by significantly decreasing the total and free activity of β-glucuronidase and inhibiting the rate of release of the enzyme from lysosomal rich fraction. Histopathological studies confirmed the above results by showing a decrease in mononuclear cell infiltration, hypertrophy, hyperplasia and pannus formation after PEP1261 treatment in arthritic rats.
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a novel Peptide Derivative exhibits anti inflammatory and antioxidant activity in adjuvant induced arthritis in rats
Molecular and Cellular Biochemistry, 2002Co-Authors: Dilly Ashok Kumar, Panchatcharam Manikandan, Miriyala Sumitra, Chandrasekar Gayathri, Natarajan Arutselvan, Kalidindi Narayana V S Raju, Rengarajulu PuvanakrishnanAbstract:A tetraPeptide Derivative PEP1261 {Boc-Lys-(Boc)-Arg-Asp-Ser-(tBu)-OtBu}, corresponding to residues 39–42 of human lactoferrin, was tested for its antiinflammatory action in adjuvant induced arthritis in rats. Administration of heat killed Mycobacterium tuberculosis (500 μg/0.1 ml of paraffin oil) intradermally into the foot pad of right hind paw resulted in an increased paw volume and an increase in the levels of reactive oxygen species and β-glucuronidase as well as a decrease in the antioxidants levels. PEP1261, at an effective dose of 10 mg/kg body wt., exhibited a significant antiarthritic activity as evidenced by lowering of paw volume and inhibited the free radicals toxicity by increasing the antioxidants levels. This Peptide Derivative was also shown to have a membrane stabilizing action by significantly decreasing the total and free activity of β-glucuronidase and inhibiting the rate of release of the enzyme from lysosomal rich fraction. Histopathological studies confirmed the above results by showing a decrease in mononuclear cell infiltration, hypertrophy, hyperplasia and pannus formation after PEP1261 treatment in arthritic rats.
Wenxin Li - One of the best experts on this subject based on the ideXlab platform.
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antibacterial activity and mechanism of a scorpion venom Peptide Derivative in vitro and in vivo
PLOS ONE, 2012Co-Authors: Zhongjie Li, Yu Song, Yingliang Wu, Wenxin LiAbstract:BmKn2 is an antimicrobial Peptide (AMP) characterized from the venom of scorpion Mesobuthus martensii Karsch by our group. In this study, Kn2-7 was derived from BmKn2 to improve the antibacterial activity and decrease hemolytic activity. Kn2-7 showed increased inhibitory activity against both Gram-positive bacteria and Gram-negative bacteria. Moreover, Kn2-7 exhibited higher antibacterial activity against clinical antibiotic-resistant strains such as methicillin-resistant Staphylococcus aureus (MRSA). In addition, the topical use of Kn2-7 effectively protected the skin of mice from infection in an S. aureus mouse skin infection model. Kn2-7 exerted its antibacterial activity via a bactericidal mechanism. Kn2-7 killed S. aureus and E. coli rapidly by binding to the lipoteichoic acid (LTA) in the S. aureus cell wall and the lipopolysaccharides (LPS) in the E. coli cell wall, respectively. Finally, the hemolytic activity of Kn2-7 was significantly decreased, compared to the wild-type Peptide BmKn2. Taken together, the Kn2-7 Peptide can be developed as a topical therapeutic agent for treating bacterial infections.
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anti hiv 1 activity of a new scorpion venom Peptide Derivative kn2 7
PLOS ONE, 2012Co-Authors: Yaoqing Chen, Maohua Zhong, Yan Zhang, Qiaoli Li, Jingyi Yang, Dihan Zhou, Benxia He, Jie Yu, Yaoming Li, Wenxin LiAbstract:For over 30 years, HIV/AIDS has wreaked havoc in the world. In the absence of an effective vaccine for HIV, development of new anti-HIV agents is urgently needed. We previously identified the antiviral activities of the scorpion-venom-Peptide-derived mucroporin-M1 for three RNA viruses (measles viruses, SARS-CoV, and H5N1). In this investigation, a panel of scorpion venom Peptides and their Derivatives were designed and chosen for assessment of their anti-HIV activities. A new scorpion venom Peptide Derivative Kn2-7 was identified as the most potent anti-HIV-1 Peptide by screening assays with an EC50 value of 2.76 µg/ml (1.65 µM) and showed low cytotoxicity to host cells with a selective index (SI) of 13.93. Kn2-7 could inhibit all members of a standard reference panel of HIV-1 subtype B pseudotyped virus (PV) with CCR5-tropic and CXCR4-tropic NL4-3 PV strain. Furthermore, it also inhibited a CXCR4-tropic replication-competent strain of HIV-1 subtype B virus. Binding assay of Kn2-7 to HIV-1 PV by Octet Red system suggested the anti-HIV-1 activity was correlated with a direct interaction between Kn2-7 and HIV-1 envelope. These results demonstrated that Peptide Kn2-7 could inhibit HIV-1 by direct interaction with viral particle and may become a promising candidate compound for further development of microbicide against HIV-1.
Miriyala Sumitra - One of the best experts on this subject based on the ideXlab platform.
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A novel Peptide Derivative exhibits anti inflammatory and antioxidant activity in adjuvant induced arthritis in rats.
Molecular and cellular biochemistry, 2020Co-Authors: Dilly Ashok Kumar, Panchatcharam Manikandan, Miriyala Sumitra, Chandrasekar Gayathri, Natarajan Arutselvan, Kalidindi V S Narayana Raju, Rengarajulu PuvanakrishnanAbstract:A tetraPeptide Derivative PEP1261 [Boc-Lys-(Boc)-Arg-Asp-Ser-(tBu)-OtBu], corresponding to residues 39-42 of human lactoferrin, was tested for its antiinflammatory action in adjuvant induced arthritis in rats. Administration of heat killed Mycobacterium tuberculosis (500 microg/0.1 ml of paraffin oil) intradermally into the foot pad of right hind paw resulted in an increased paw volume and an increase in the levels of reactive oxygen species and beta-glucuronidase as well as a decrease in the antioxidants levels. PEP1261, at an effective dose of 10 mg/kg body wt., exhibited a significant antiarthritic activity as evidenced by lowering of paw volume and inhibited the free radicals toxicity by increasing the antioxidants levels. This Peptide Derivative was also shown to have a membrane stabilizing action by significantly decreasing the total and free activity of beta-glucuronidase and inhibiting the rate of release of the enzyme from lysosomal rich fraction. Histopathological studies confirmed the above results by showing a decrease in mononuclear cell infiltration, hypertrophy, hyperplasia and pannus formation after PEP1261 treatment in arthritic rats.
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A novel Peptide Derivative exhibits antiinflammatory and antioxidant activity in adjuvant induced arthritis in rats
Molecular and Cellular Biochemistry, 2002Co-Authors: Dilly Ashok Kumar, Panchatcharam Manikandan, Miriyala Sumitra, Kalidindi V.s. Narayana Raju, Chandrasekar Gayathri, Natarajan Arutselvan, Rengarajulu PuvanakrishnanAbstract:A tetraPeptide Derivative PEP1261 {Boc-Lys-(Boc)-Arg-Asp-Ser-(tBu)-OtBu}, corresponding to residues 39–42 of human lactoferrin, was tested for its antiinflammatory action in adjuvant induced arthritis in rats. Administration of heat killed Mycobacterium tuberculosis (500 μg/0.1 ml of paraffin oil) intradermally into the foot pad of right hind paw resulted in an increased paw volume and an increase in the levels of reactive oxygen species and β-glucuronidase as well as a decrease in the antioxidants levels. PEP1261, at an effective dose of 10 mg/kg body wt., exhibited a significant antiarthritic activity as evidenced by lowering of paw volume and inhibited the free radicals toxicity by increasing the antioxidants levels. This Peptide Derivative was also shown to have a membrane stabilizing action by significantly decreasing the total and free activity of β-glucuronidase and inhibiting the rate of release of the enzyme from lysosomal rich fraction. Histopathological studies confirmed the above results by showing a decrease in mononuclear cell infiltration, hypertrophy, hyperplasia and pannus formation after PEP1261 treatment in arthritic rats.
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a novel Peptide Derivative exhibits anti inflammatory and antioxidant activity in adjuvant induced arthritis in rats
Molecular and Cellular Biochemistry, 2002Co-Authors: Dilly Ashok Kumar, Panchatcharam Manikandan, Miriyala Sumitra, Chandrasekar Gayathri, Natarajan Arutselvan, Kalidindi Narayana V S Raju, Rengarajulu PuvanakrishnanAbstract:A tetraPeptide Derivative PEP1261 {Boc-Lys-(Boc)-Arg-Asp-Ser-(tBu)-OtBu}, corresponding to residues 39–42 of human lactoferrin, was tested for its antiinflammatory action in adjuvant induced arthritis in rats. Administration of heat killed Mycobacterium tuberculosis (500 μg/0.1 ml of paraffin oil) intradermally into the foot pad of right hind paw resulted in an increased paw volume and an increase in the levels of reactive oxygen species and β-glucuronidase as well as a decrease in the antioxidants levels. PEP1261, at an effective dose of 10 mg/kg body wt., exhibited a significant antiarthritic activity as evidenced by lowering of paw volume and inhibited the free radicals toxicity by increasing the antioxidants levels. This Peptide Derivative was also shown to have a membrane stabilizing action by significantly decreasing the total and free activity of β-glucuronidase and inhibiting the rate of release of the enzyme from lysosomal rich fraction. Histopathological studies confirmed the above results by showing a decrease in mononuclear cell infiltration, hypertrophy, hyperplasia and pannus formation after PEP1261 treatment in arthritic rats.
Natarajan Arutselvan - One of the best experts on this subject based on the ideXlab platform.
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A novel Peptide Derivative exhibits anti inflammatory and antioxidant activity in adjuvant induced arthritis in rats.
Molecular and cellular biochemistry, 2020Co-Authors: Dilly Ashok Kumar, Panchatcharam Manikandan, Miriyala Sumitra, Chandrasekar Gayathri, Natarajan Arutselvan, Kalidindi V S Narayana Raju, Rengarajulu PuvanakrishnanAbstract:A tetraPeptide Derivative PEP1261 [Boc-Lys-(Boc)-Arg-Asp-Ser-(tBu)-OtBu], corresponding to residues 39-42 of human lactoferrin, was tested for its antiinflammatory action in adjuvant induced arthritis in rats. Administration of heat killed Mycobacterium tuberculosis (500 microg/0.1 ml of paraffin oil) intradermally into the foot pad of right hind paw resulted in an increased paw volume and an increase in the levels of reactive oxygen species and beta-glucuronidase as well as a decrease in the antioxidants levels. PEP1261, at an effective dose of 10 mg/kg body wt., exhibited a significant antiarthritic activity as evidenced by lowering of paw volume and inhibited the free radicals toxicity by increasing the antioxidants levels. This Peptide Derivative was also shown to have a membrane stabilizing action by significantly decreasing the total and free activity of beta-glucuronidase and inhibiting the rate of release of the enzyme from lysosomal rich fraction. Histopathological studies confirmed the above results by showing a decrease in mononuclear cell infiltration, hypertrophy, hyperplasia and pannus formation after PEP1261 treatment in arthritic rats.
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A novel Peptide Derivative exhibits antiinflammatory and antioxidant activity in adjuvant induced arthritis in rats
Molecular and Cellular Biochemistry, 2002Co-Authors: Dilly Ashok Kumar, Panchatcharam Manikandan, Miriyala Sumitra, Kalidindi V.s. Narayana Raju, Chandrasekar Gayathri, Natarajan Arutselvan, Rengarajulu PuvanakrishnanAbstract:A tetraPeptide Derivative PEP1261 {Boc-Lys-(Boc)-Arg-Asp-Ser-(tBu)-OtBu}, corresponding to residues 39–42 of human lactoferrin, was tested for its antiinflammatory action in adjuvant induced arthritis in rats. Administration of heat killed Mycobacterium tuberculosis (500 μg/0.1 ml of paraffin oil) intradermally into the foot pad of right hind paw resulted in an increased paw volume and an increase in the levels of reactive oxygen species and β-glucuronidase as well as a decrease in the antioxidants levels. PEP1261, at an effective dose of 10 mg/kg body wt., exhibited a significant antiarthritic activity as evidenced by lowering of paw volume and inhibited the free radicals toxicity by increasing the antioxidants levels. This Peptide Derivative was also shown to have a membrane stabilizing action by significantly decreasing the total and free activity of β-glucuronidase and inhibiting the rate of release of the enzyme from lysosomal rich fraction. Histopathological studies confirmed the above results by showing a decrease in mononuclear cell infiltration, hypertrophy, hyperplasia and pannus formation after PEP1261 treatment in arthritic rats.
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a novel Peptide Derivative exhibits anti inflammatory and antioxidant activity in adjuvant induced arthritis in rats
Molecular and Cellular Biochemistry, 2002Co-Authors: Dilly Ashok Kumar, Panchatcharam Manikandan, Miriyala Sumitra, Chandrasekar Gayathri, Natarajan Arutselvan, Kalidindi Narayana V S Raju, Rengarajulu PuvanakrishnanAbstract:A tetraPeptide Derivative PEP1261 {Boc-Lys-(Boc)-Arg-Asp-Ser-(tBu)-OtBu}, corresponding to residues 39–42 of human lactoferrin, was tested for its antiinflammatory action in adjuvant induced arthritis in rats. Administration of heat killed Mycobacterium tuberculosis (500 μg/0.1 ml of paraffin oil) intradermally into the foot pad of right hind paw resulted in an increased paw volume and an increase in the levels of reactive oxygen species and β-glucuronidase as well as a decrease in the antioxidants levels. PEP1261, at an effective dose of 10 mg/kg body wt., exhibited a significant antiarthritic activity as evidenced by lowering of paw volume and inhibited the free radicals toxicity by increasing the antioxidants levels. This Peptide Derivative was also shown to have a membrane stabilizing action by significantly decreasing the total and free activity of β-glucuronidase and inhibiting the rate of release of the enzyme from lysosomal rich fraction. Histopathological studies confirmed the above results by showing a decrease in mononuclear cell infiltration, hypertrophy, hyperplasia and pannus formation after PEP1261 treatment in arthritic rats.