The Experts below are selected from a list of 267 Experts worldwide ranked by ideXlab platform

Knud J. Jensen - One of the best experts on this subject based on the ideXlab platform.

  • Linkers, resins, and general procedures for solid-phase Peptide Synthesis.
    Methods in molecular biology (Clifton N.J.), 2013
    Co-Authors: Pernille Tofteng Shelton, Knud J. Jensen
    Abstract:

    This chapter describes the basic protocols for solid-phase Peptide Synthesis using the Fmoc group as the N (α)-protecting group (Fmoc-SPPS). The chapter introduces resins and their handling, choice of linkers, and the most common methods for Peptide chain assembly. The proper choice of resins and linkers for solid-phase Synthesis is a key parameter for successful Peptide Synthesis. This chapter provides an overview of the most common and useful resins and linkers for the Synthesis of Peptides with C-terminal amides, carboxylic acids, and more. The chapter finishes with robust protocols for general solid-phase Peptide Synthesis, i.e., the standard operations.

  • Solid-phase Peptide Synthesis: an introduction.
    Methods in molecular biology (Clifton N.J.), 2013
    Co-Authors: Knud J. Jensen
    Abstract:

    This chapter provides an introduction to and overview of Peptide chemistry with a focus on solid-phase Peptide Synthesis. The background, the most common reagents, and some mechanisms are presented. This chapter also points to the different chapters and puts them into perspective.

Alberto Oddo - One of the best experts on this subject based on the ideXlab platform.

  • Fmoc Solid-Phase Peptide Synthesis.
    Methods in molecular biology (Clifton N.J.), 2015
    Co-Authors: Paul R. Hansen, Alberto Oddo
    Abstract:

    Synthetic Peptides are important as drugs and in research. Currently, the method of choice for producing these compounds is solid-phase Peptide Synthesis. In this nonspecialist review, we describe the scope and limitations of Fmoc solid-phase Peptide Synthesis. Furthermore, we provide a detailed protocol for Fmoc Peptide Synthesis.

Gregg B. Fields - One of the best experts on this subject based on the ideXlab platform.

  • Solid phase Peptide Synthesis utilizing 9‐fluorenylmethoxycarbonyl amino acids
    International journal of peptide and protein research, 2009
    Co-Authors: Gregg B. Fields, Richard L. Noble
    Abstract:

    9-Fluorenylmethoxycarbonyl (Fmoc) amino acids were first used for solid phase Peptide Synthesis a little more than a decade ago. Since that time, Fmoc solid phase Peptide Synthesis methodology has been greatly enhanced by the introduction of a variety of solid supports, linkages, and side chain protecting groups, as well as by increased understanding of solvation conditions. These advances have led to many impressive syntheses, such as those of biologically active and isotopically labeled Peptides and small proteins. The great variety of conditions under which Fmoc solid phase Peptide Synthesis may be carried out represents a truly "orthogonal" scheme, and thus offers many unique opportunities for bioorganic chemistry.

  • UNIT 18.1 Introduction to Peptide Synthesis
    Current protocols in immunology, 2002
    Co-Authors: Gregg B. Fields
    Abstract:

    This overview presents a review of classical and current solid-phase Peptide Synthesis techniques. Peptide Synthesis has become a more practical part of present-day scientific research following the advent of solid-phase techniques, and has practical commercial and scientific implications. This unit provides information on solid-phase Peptide-Synthesis methodology, offering descriptions of different supports, coupling reagents, and practical approaches to the Synthesis of modified residues and structures. It also presents information concerning protein Synthesis and the purification and analysis of synthetic Peptides. This methodology has great implications for the design of synthetic Peptides to serve in antibody production.

Srinivasan Chandrasekaran - One of the best experts on this subject based on the ideXlab platform.

  • Applications of Propargyl Esters of Amino Acids in Solution-Phase Peptide Synthesis
    International journal of peptides, 2011
    Co-Authors: Ramesh Ramapanicker, Rohit Gupta, Rajendran Megha, Srinivasan Chandrasekaran
    Abstract:

    Propargyl esters are employed as effective protecting groups for the carboxyl group during solution-phase Peptide Synthesis. The propargyl ester groups can be introduced onto free amino acids by treating them with propargyl alcohol saturated with HCl. The reaction between propargyl groups and tetrathiomolybdate is exploited to deblock the propargyl esters. The removal of the propargyl group with the neutral reagent tetrathiomolybdate ensures that most of the other protecting groups used in Peptide Synthesis are untouched. Both acid labile and base labile protecting groups can be removed in the presence of a propargyl ester. Amino acids protected as propargyl esters are employed to synthesize di- to tetraPeptides in solution-phase demonstrating the possible synthetic utilities of the methodology. The methodology described here could be a valuable addition to currently available strategies for Peptide Synthesis.

  • Synthesis and applications of propargyl pentafluorophenyl carbonate for Peptide Synthesis
    Tetrahedron Letters, 2002
    Co-Authors: Ramakrishna G. Bhat, Erwan Kerouredan, Emmanuel Porhiel, Srinivasan Chandrasekaran
    Abstract:

    Propargyl pentafluorophenyl carbonate was Synthesised in quantitative yield by the reaction of propargyl chloroformate and pentafluorophenol. All the N-propargyloxycarbonyl (N-Poc) amino acids were obtained in good yield. The use of Poc-OPfp in Peptide Synthesis has been explored.

Les P. Miranda - One of the best experts on this subject based on the ideXlab platform.

  • Solid-phase Peptide Synthesis using microwave irradiation.
    Methods in molecular biology (Clifton N.J.), 2011
    Co-Authors: Justin K. Murray, Jennifer Aral, Les P. Miranda
    Abstract:

    Since the advent of solid-phase Peptide Synthesis (SPPS) in the late 1950s, numerous advancements in the underlying chemistry (i.e., orthogonal protection strategy, coupling reagents, and solid support matrices) have greatly improved the efficiency of the technique. More recently, application of microwave radiation to SPPS has been found to reduce reaction time and/or increase the initial purity of synthetic Peptide products. In this protocol, conditions are described to accomplish rapid Peptide coupling and 9-fluorenylmethoxycarbonyl (Fmoc) removal reactions under temperature-controlled conditions in either a manual or automated Synthesis format using a microwave reactor. These microwave-assisted Peptide Synthesis procedures have been used to rapidly prepare a "difficult" Peptide sequence from the acyl carrier protein, ACP(65-74), in less than 3 h and the reduced, linear precursor to human hepcidin, in high initial purity.