The Experts below are selected from a list of 282 Experts worldwide ranked by ideXlab platform
A Carpentier - One of the best experts on this subject based on the ideXlab platform.
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gal knockout pig Pericardium new source of material for heart valve bioprostheses
Journal of Heart and Lung Transplantation, 2010Co-Authors: Nermine Lila, Christopher G A Mcgregor, Sophie Carpentier, Jeanne Rancic, Guerard W Byrne, A CarpentierAbstract:BACKGROUND: Although glutaraldehyde fixation is known to reduce immunogenicity and degeneration of heart valve bioprostheses, some degree of immunogenicity persists, which may trigger calcification. The aims of this study were to: (1) define the role of alpha-1,3-galactosyltransferase (alpha-Gal) antigen in valve calcification by comparing alpha-Gal-positive and alpha-Gal-deficient (GT-KO) pig Pericardium; and (2) elucidate the role of human anti-Gal antibodies in the process of calcification and to determine the potential influence of different tissue-fixation techniques. METHODS: Glutaraldehyde-treated Pericardium from alpha-Gal-positive and GT-KO pigs, with or without pre-labeling with human anti-Gal antibodies, were implanted in rats during 1 month. RESULTS: In glutaraldehyde-fixed Pericardium, calcification levels were significantly lower in GT-KO pig Pericardium (132.8 +/- 5.8 microg/mg) as compared with alpha-Gal-positive pig Pericardium (155.7 +/- 7.1 microg/mg) (p < 0.015). In glutaraldehyde-fixed pig Pericardium followed by a mix of formaldehyde, ethanol and Tween 80 (FET), the calcification levels were lower in GT-KO pig Pericardium (0.35 +/- 0.1 microg/mg) as compared with alpha-Gal-positive pig Pericardium (4.6 +/- 4.2 microg/mg). In glutaraldehyde-fixed pig Pericardium + FET pre-incubated with human anti-Gal antibodies, calcification levels were significantly greater in alpha-Gal-positive pig Pericardium (43.8 +/- 8.5 microg/mg) as compared with GT-KO pig Pericardium (5.7 +/- 2.9 microg/mg) (p < 0.0001). CONCLUSIONS: This study demonstrates the role of alpha-Gal antigen and human alpha-Gal antibodies in the calcification process of valvular bioprostheses. It is suggested that GT-KO pig Pericardium could be beneficial as a new source of material for heart valve bioprostheses.
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gal knockout pig Pericardium new source of material for heart valve bioprostheses
Journal of Heart and Lung Transplantation, 2010Co-Authors: Nermine Lila, Christopher G A Mcgregor, Sophie Carpentier, Jeanne Rancic, Guerard W Byrne, A CarpentierAbstract:Background Although glutaraldehyde fixation is known to reduce immunogenicity and degeneration of heart valve bioprostheses, some degree of immunogenicity persists, which may trigger calcification. The aims of this study were to: (1) define the role of alpha-1,3-galactosyltransferase (α-Gal) antigen in valve calcification by comparing α-Gal–positive and α-Gal–deficient (GT-KO) pig Pericardium; and (2) elucidate the role of human anti-Gal antibodies in the process of calcification and to determine the potential influence of different tissue-fixation techniques. Methods Glutaraldehyde-treated Pericardium from α-Gal–positive and GT-KO pigs, with or without pre-labeling with human anti-Gal antibodies, were implanted in rats during 1 month. Results In glutaraldehyde-fixed Pericardium, calcification levels were significantly lower in GT-KO pig Pericardium (132.8 ± 5.8 μg/mg) as compared with α-Gal–positive pig Pericardium (155.7 ± 7.1 μg/mg) ( p p Conclusions This study demonstrates the role of α-Gal antigen and human α-Gal antibodies in the calcification process of valvular bioprostheses. It is suggested that GT-KO pig Pericardium could be beneficial as a new source of material for heart valve bioprostheses.
Nermine Lila - One of the best experts on this subject based on the ideXlab platform.
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gal knockout pig Pericardium new source of material for heart valve bioprostheses
Journal of Heart and Lung Transplantation, 2010Co-Authors: Nermine Lila, Christopher G A Mcgregor, Sophie Carpentier, Jeanne Rancic, Guerard W Byrne, A CarpentierAbstract:BACKGROUND: Although glutaraldehyde fixation is known to reduce immunogenicity and degeneration of heart valve bioprostheses, some degree of immunogenicity persists, which may trigger calcification. The aims of this study were to: (1) define the role of alpha-1,3-galactosyltransferase (alpha-Gal) antigen in valve calcification by comparing alpha-Gal-positive and alpha-Gal-deficient (GT-KO) pig Pericardium; and (2) elucidate the role of human anti-Gal antibodies in the process of calcification and to determine the potential influence of different tissue-fixation techniques. METHODS: Glutaraldehyde-treated Pericardium from alpha-Gal-positive and GT-KO pigs, with or without pre-labeling with human anti-Gal antibodies, were implanted in rats during 1 month. RESULTS: In glutaraldehyde-fixed Pericardium, calcification levels were significantly lower in GT-KO pig Pericardium (132.8 +/- 5.8 microg/mg) as compared with alpha-Gal-positive pig Pericardium (155.7 +/- 7.1 microg/mg) (p < 0.015). In glutaraldehyde-fixed pig Pericardium followed by a mix of formaldehyde, ethanol and Tween 80 (FET), the calcification levels were lower in GT-KO pig Pericardium (0.35 +/- 0.1 microg/mg) as compared with alpha-Gal-positive pig Pericardium (4.6 +/- 4.2 microg/mg). In glutaraldehyde-fixed pig Pericardium + FET pre-incubated with human anti-Gal antibodies, calcification levels were significantly greater in alpha-Gal-positive pig Pericardium (43.8 +/- 8.5 microg/mg) as compared with GT-KO pig Pericardium (5.7 +/- 2.9 microg/mg) (p < 0.0001). CONCLUSIONS: This study demonstrates the role of alpha-Gal antigen and human alpha-Gal antibodies in the calcification process of valvular bioprostheses. It is suggested that GT-KO pig Pericardium could be beneficial as a new source of material for heart valve bioprostheses.
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gal knockout pig Pericardium new source of material for heart valve bioprostheses
Journal of Heart and Lung Transplantation, 2010Co-Authors: Nermine Lila, Christopher G A Mcgregor, Sophie Carpentier, Jeanne Rancic, Guerard W Byrne, A CarpentierAbstract:Background Although glutaraldehyde fixation is known to reduce immunogenicity and degeneration of heart valve bioprostheses, some degree of immunogenicity persists, which may trigger calcification. The aims of this study were to: (1) define the role of alpha-1,3-galactosyltransferase (α-Gal) antigen in valve calcification by comparing α-Gal–positive and α-Gal–deficient (GT-KO) pig Pericardium; and (2) elucidate the role of human anti-Gal antibodies in the process of calcification and to determine the potential influence of different tissue-fixation techniques. Methods Glutaraldehyde-treated Pericardium from α-Gal–positive and GT-KO pigs, with or without pre-labeling with human anti-Gal antibodies, were implanted in rats during 1 month. Results In glutaraldehyde-fixed Pericardium, calcification levels were significantly lower in GT-KO pig Pericardium (132.8 ± 5.8 μg/mg) as compared with α-Gal–positive pig Pericardium (155.7 ± 7.1 μg/mg) ( p p Conclusions This study demonstrates the role of α-Gal antigen and human α-Gal antibodies in the calcification process of valvular bioprostheses. It is suggested that GT-KO pig Pericardium could be beneficial as a new source of material for heart valve bioprostheses.
Frederick J. Schoen - One of the best experts on this subject based on the ideXlab platform.
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Prevention of calcification of glutaraldehyde pretreated bovine Pericardium through controlled release polymeric implants: studies of Fe3+, Al3+, protamine sulphate and levamisole.
Biomaterials, 1990Co-Authors: Yashwant V. Pathak, Robert J. Levy, James A. Boyd, Frederick J. SchoenAbstract:Abstract Calcification is the principal cause of the clinical failure of bioprosthetic heart valves fabricated from glutaraldehyde pretreated porcine aortic valves or bovine Pericardium. The present study investigated controlled-release implants for prevention of the calcification of glutaraldehyde pretreated bovine Pericardium in a rat subdermal model. Either Al 3+ and Fe 3+ (inhibitors of the growth and dissolution rate of hydroxyapatite crystals), levamisole (alkaline phosphatase inhibitor) or protamine sulphate (charge modifier) were individually incorporated into various polymeric carriers (either silicone rubber. Polyurethane or silicone rubber-polyurethane copolymer). Polymeric implants were evaluated for in vitro release kinetics, which revealed that sustained drug release was obtained from 21 d to more than 90 d from various drug matrices. In vivo efficacy was studied by co-implanting the polymeric delivery systems with glutaraldehyde pretreated bovine Pericardium for 21 d using a subdermal rat model; glutaraldehyde pretreated bovine Pericardium calcium levels were quantitated by atomic absorption spectroscopy in the explanted tissues. Fe 3+ and Al 3+ polymeric implants were the most effective for inhibiting deposition of calcium mineral. Al 3+ demonstrated 82% inhibition of calcification compared to controls and Fe 3+ resulted in 80% inhibition of calcification. Specific histologie staining methods showed that Fe 3+ and Al 3+ were localized within the devitalized cells of the explanted glutaraldehyde pretreated bovine Pericardium. No adverse effects on somatic growth or recipient bone morphology were noted following controlled-release drug administration. Controlled release of protamine sulphate or levamisole did not significantly inhibit glutaraldehyde pretreated bovine Pericardium calcification. It is concluded that regional controlled release of Fe 3+ or AI 3+ inhibits glutaraldehyde pretreated bovine Pericardium calcification in the rat subdermal model without adverse effects.
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Prevention of calcification of glutaraldehyde pretreated bovine Pericardium through controlled release polymeric implants
1990Co-Authors: Yashwant Pathak, James H. Boyd, Robert J. Levy, Frederick J. SchoenAbstract:Calcification is the principal cause of the clinical failure of bioprosthetic heart valves fabricated from glutaraldehyde pretreated porcine aortic valves or bovine Pericardium. The present study investigated controlled-release implants for prevention of the calcification of glutaraldehyde pretreated bovine Pericardium in a rat subdermal model. Either A13+ and Fe3+ (inhibitors of the growth and dissolution rate of hydroxyapatite crystals), levamisole (alkaline phosphatase inhibitor) or protamine sulphate (charge modifier) were individually incorporated into various polymeric carriers (either silicone rubber, polyurethane or silicone rubber-polyurethane copolymer). Polymeric implants were evaluated for in vitro release kinetics, which revealed that sustained drug release was obtained from 21 d to more than 90 d from various drug matrices. In viva efficacy was studied by co-implanting the polymeric delivery systems with glutaraldehyde pretreated bovine Pericardium for 21 d using a subdermal rat model; glutaraldehyde pretreated bovine Pericardium calcium levels were quantitated by atomic absorption spectroscopy in the explanted tissues. Fe'+ and A13+ polymeric implants were the most effective for inhibiting deposition of calcium mineral. AI'+ demonstrated 82% inhibition of calcification compared to controls and Fe3+ resulted in 80% inhibition of calcification. Specific histologic staining methods showed that Fe3+ and A13+ were localized within the devitalized cells of the explanted glutaraldehyde pretreated bovine Pericardium. No adverse effects on somatic growth or recipient bone morphology were noted following controlled-release drug administration. Controlled release of protamine sulphate or levamisole did not significantly inhibit glutaraldehyde pretreated bovine Pericardium calcification. It is concluded that regional controlled release of Fe3+ or A13+ inhibits glutaraldehyde pretreated bovine Pericardium calcification in the rat subdermal model without adverse effects.
Sophie Carpentier - One of the best experts on this subject based on the ideXlab platform.
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gal knockout pig Pericardium new source of material for heart valve bioprostheses
Journal of Heart and Lung Transplantation, 2010Co-Authors: Nermine Lila, Christopher G A Mcgregor, Sophie Carpentier, Jeanne Rancic, Guerard W Byrne, A CarpentierAbstract:BACKGROUND: Although glutaraldehyde fixation is known to reduce immunogenicity and degeneration of heart valve bioprostheses, some degree of immunogenicity persists, which may trigger calcification. The aims of this study were to: (1) define the role of alpha-1,3-galactosyltransferase (alpha-Gal) antigen in valve calcification by comparing alpha-Gal-positive and alpha-Gal-deficient (GT-KO) pig Pericardium; and (2) elucidate the role of human anti-Gal antibodies in the process of calcification and to determine the potential influence of different tissue-fixation techniques. METHODS: Glutaraldehyde-treated Pericardium from alpha-Gal-positive and GT-KO pigs, with or without pre-labeling with human anti-Gal antibodies, were implanted in rats during 1 month. RESULTS: In glutaraldehyde-fixed Pericardium, calcification levels were significantly lower in GT-KO pig Pericardium (132.8 +/- 5.8 microg/mg) as compared with alpha-Gal-positive pig Pericardium (155.7 +/- 7.1 microg/mg) (p < 0.015). In glutaraldehyde-fixed pig Pericardium followed by a mix of formaldehyde, ethanol and Tween 80 (FET), the calcification levels were lower in GT-KO pig Pericardium (0.35 +/- 0.1 microg/mg) as compared with alpha-Gal-positive pig Pericardium (4.6 +/- 4.2 microg/mg). In glutaraldehyde-fixed pig Pericardium + FET pre-incubated with human anti-Gal antibodies, calcification levels were significantly greater in alpha-Gal-positive pig Pericardium (43.8 +/- 8.5 microg/mg) as compared with GT-KO pig Pericardium (5.7 +/- 2.9 microg/mg) (p < 0.0001). CONCLUSIONS: This study demonstrates the role of alpha-Gal antigen and human alpha-Gal antibodies in the calcification process of valvular bioprostheses. It is suggested that GT-KO pig Pericardium could be beneficial as a new source of material for heart valve bioprostheses.
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gal knockout pig Pericardium new source of material for heart valve bioprostheses
Journal of Heart and Lung Transplantation, 2010Co-Authors: Nermine Lila, Christopher G A Mcgregor, Sophie Carpentier, Jeanne Rancic, Guerard W Byrne, A CarpentierAbstract:Background Although glutaraldehyde fixation is known to reduce immunogenicity and degeneration of heart valve bioprostheses, some degree of immunogenicity persists, which may trigger calcification. The aims of this study were to: (1) define the role of alpha-1,3-galactosyltransferase (α-Gal) antigen in valve calcification by comparing α-Gal–positive and α-Gal–deficient (GT-KO) pig Pericardium; and (2) elucidate the role of human anti-Gal antibodies in the process of calcification and to determine the potential influence of different tissue-fixation techniques. Methods Glutaraldehyde-treated Pericardium from α-Gal–positive and GT-KO pigs, with or without pre-labeling with human anti-Gal antibodies, were implanted in rats during 1 month. Results In glutaraldehyde-fixed Pericardium, calcification levels were significantly lower in GT-KO pig Pericardium (132.8 ± 5.8 μg/mg) as compared with α-Gal–positive pig Pericardium (155.7 ± 7.1 μg/mg) ( p p Conclusions This study demonstrates the role of α-Gal antigen and human α-Gal antibodies in the calcification process of valvular bioprostheses. It is suggested that GT-KO pig Pericardium could be beneficial as a new source of material for heart valve bioprostheses.
Guerard W Byrne - One of the best experts on this subject based on the ideXlab platform.
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gal knockout pig Pericardium new source of material for heart valve bioprostheses
Journal of Heart and Lung Transplantation, 2010Co-Authors: Nermine Lila, Christopher G A Mcgregor, Sophie Carpentier, Jeanne Rancic, Guerard W Byrne, A CarpentierAbstract:BACKGROUND: Although glutaraldehyde fixation is known to reduce immunogenicity and degeneration of heart valve bioprostheses, some degree of immunogenicity persists, which may trigger calcification. The aims of this study were to: (1) define the role of alpha-1,3-galactosyltransferase (alpha-Gal) antigen in valve calcification by comparing alpha-Gal-positive and alpha-Gal-deficient (GT-KO) pig Pericardium; and (2) elucidate the role of human anti-Gal antibodies in the process of calcification and to determine the potential influence of different tissue-fixation techniques. METHODS: Glutaraldehyde-treated Pericardium from alpha-Gal-positive and GT-KO pigs, with or without pre-labeling with human anti-Gal antibodies, were implanted in rats during 1 month. RESULTS: In glutaraldehyde-fixed Pericardium, calcification levels were significantly lower in GT-KO pig Pericardium (132.8 +/- 5.8 microg/mg) as compared with alpha-Gal-positive pig Pericardium (155.7 +/- 7.1 microg/mg) (p < 0.015). In glutaraldehyde-fixed pig Pericardium followed by a mix of formaldehyde, ethanol and Tween 80 (FET), the calcification levels were lower in GT-KO pig Pericardium (0.35 +/- 0.1 microg/mg) as compared with alpha-Gal-positive pig Pericardium (4.6 +/- 4.2 microg/mg). In glutaraldehyde-fixed pig Pericardium + FET pre-incubated with human anti-Gal antibodies, calcification levels were significantly greater in alpha-Gal-positive pig Pericardium (43.8 +/- 8.5 microg/mg) as compared with GT-KO pig Pericardium (5.7 +/- 2.9 microg/mg) (p < 0.0001). CONCLUSIONS: This study demonstrates the role of alpha-Gal antigen and human alpha-Gal antibodies in the calcification process of valvular bioprostheses. It is suggested that GT-KO pig Pericardium could be beneficial as a new source of material for heart valve bioprostheses.
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gal knockout pig Pericardium new source of material for heart valve bioprostheses
Journal of Heart and Lung Transplantation, 2010Co-Authors: Nermine Lila, Christopher G A Mcgregor, Sophie Carpentier, Jeanne Rancic, Guerard W Byrne, A CarpentierAbstract:Background Although glutaraldehyde fixation is known to reduce immunogenicity and degeneration of heart valve bioprostheses, some degree of immunogenicity persists, which may trigger calcification. The aims of this study were to: (1) define the role of alpha-1,3-galactosyltransferase (α-Gal) antigen in valve calcification by comparing α-Gal–positive and α-Gal–deficient (GT-KO) pig Pericardium; and (2) elucidate the role of human anti-Gal antibodies in the process of calcification and to determine the potential influence of different tissue-fixation techniques. Methods Glutaraldehyde-treated Pericardium from α-Gal–positive and GT-KO pigs, with or without pre-labeling with human anti-Gal antibodies, were implanted in rats during 1 month. Results In glutaraldehyde-fixed Pericardium, calcification levels were significantly lower in GT-KO pig Pericardium (132.8 ± 5.8 μg/mg) as compared with α-Gal–positive pig Pericardium (155.7 ± 7.1 μg/mg) ( p p Conclusions This study demonstrates the role of α-Gal antigen and human α-Gal antibodies in the calcification process of valvular bioprostheses. It is suggested that GT-KO pig Pericardium could be beneficial as a new source of material for heart valve bioprostheses.
