The Experts below are selected from a list of 51 Experts worldwide ranked by ideXlab platform
Uwe Klinge - One of the best experts on this subject based on the ideXlab platform.
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Influence of 4% icodextrin solution on peritoneal tissue response and Adhesion formation.
BMC surgery, 2013Co-Authors: Christian D. Klink, Patrick Schickhaus, Marcel Binnebösel, Stefan Jockenhoevel, Rafael Rosch, Rene Tolba, Ulf P. Neumann, Uwe KlingeAbstract:Background Postoperative peritoneal Adhesion formation following abdominal surgery remains a relevant surgical problem. The application of soluble physico-chemical barriers like 4% icodextrin is one approach to protect the peritoneal surface from getting linked to adhesive scar. The aim of this study was to investigate the influence of 4% icodextrin on peritoneal tissue response both of visceral and parietal Peritoneum, Adhesion formation and wound healing.
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Influence of 4% icodextrin solution on peritoneal tissue response and Adhesion formation
BMC Surgery, 2013Co-Authors: Christian D. Klink, Patrick Schickhaus, Marcel Binnebösel, Stefan Jockenhoevel, Rafael Rosch, Rene Tolba, Ulf P. Neumann, Uwe KlingeAbstract:Background Postoperative peritoneal Adhesion formation following abdominal surgery remains a relevant surgical problem. The application of soluble physico-chemical barriers like 4% icodextrin is one approach to protect the peritoneal surface from getting linked to adhesive scar. The aim of this study was to investigate the influence of 4% icodextrin on peritoneal tissue response both of visceral and parietal Peritoneum, Adhesion formation and wound healing. Methods 40 rats were divided into two groups. After creation of an intraabdominal defect, either 4% icodextrin (Adept®) or sodium chloride was applied. Animals were sacrificed after 7 and 21 days. Adhesions were scored by an Adhesion score. Furthermore, immunohistochemical investigations were conducted to determine the discrete influence of icodextrin on the parietal and visceral peritoneal tissue responses (CD68^+ macrophages, CD3^+ T-lymphocytes, vimentin for mesenchymal cells, HBME-1 for mesothelial cells, and as components of wound healing COX-2, C-myc, catenin). Results Postoperative peritoneal Adhesions were predominantly present in the sodium chloride group as compared to the icodextrin group (14/19 (74%) vs. 9/19 (47%); p = 0.048). The Adhesion score however did not reveal any significant differences, (p = 0.614). Furthermore, the expression of vimentin in both the parietal and visceral Peritoneum after 21 days was significantly lower in the icodextrin group than in the sodium chloride group (p = 0.038 and p = 0.028, respectively). No significant differences were observed for macrophages, lymphocytes, reperitonealisation or the expression of COX-2, C-myc or Catenin. Conclusions The intraperitoneal application of 4% icodextrin reduces Adhesion formation in comparison to sodium chloride. 4% icodextrin solution reduces the inflammatory and mesenchymal infiltrate in the wounded area, thus improving the ratio of mesothel cells to mesenchymal infiltrate. As demonstrated, icodextrin is able to ameliorate the local tissue response. Further experimental studies would be done to elaborate the impact on the early response of the adaptive immune system, which may then trigger the subsequent wound healing and tissue repair.
Christian D. Klink - One of the best experts on this subject based on the ideXlab platform.
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Influence of 4% icodextrin solution on peritoneal tissue response and Adhesion formation.
BMC surgery, 2013Co-Authors: Christian D. Klink, Patrick Schickhaus, Marcel Binnebösel, Stefan Jockenhoevel, Rafael Rosch, Rene Tolba, Ulf P. Neumann, Uwe KlingeAbstract:Background Postoperative peritoneal Adhesion formation following abdominal surgery remains a relevant surgical problem. The application of soluble physico-chemical barriers like 4% icodextrin is one approach to protect the peritoneal surface from getting linked to adhesive scar. The aim of this study was to investigate the influence of 4% icodextrin on peritoneal tissue response both of visceral and parietal Peritoneum, Adhesion formation and wound healing.
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Influence of 4% icodextrin solution on peritoneal tissue response and Adhesion formation
BMC Surgery, 2013Co-Authors: Christian D. Klink, Patrick Schickhaus, Marcel Binnebösel, Stefan Jockenhoevel, Rafael Rosch, Rene Tolba, Ulf P. Neumann, Uwe KlingeAbstract:Background Postoperative peritoneal Adhesion formation following abdominal surgery remains a relevant surgical problem. The application of soluble physico-chemical barriers like 4% icodextrin is one approach to protect the peritoneal surface from getting linked to adhesive scar. The aim of this study was to investigate the influence of 4% icodextrin on peritoneal tissue response both of visceral and parietal Peritoneum, Adhesion formation and wound healing. Methods 40 rats were divided into two groups. After creation of an intraabdominal defect, either 4% icodextrin (Adept®) or sodium chloride was applied. Animals were sacrificed after 7 and 21 days. Adhesions were scored by an Adhesion score. Furthermore, immunohistochemical investigations were conducted to determine the discrete influence of icodextrin on the parietal and visceral peritoneal tissue responses (CD68^+ macrophages, CD3^+ T-lymphocytes, vimentin for mesenchymal cells, HBME-1 for mesothelial cells, and as components of wound healing COX-2, C-myc, catenin). Results Postoperative peritoneal Adhesions were predominantly present in the sodium chloride group as compared to the icodextrin group (14/19 (74%) vs. 9/19 (47%); p = 0.048). The Adhesion score however did not reveal any significant differences, (p = 0.614). Furthermore, the expression of vimentin in both the parietal and visceral Peritoneum after 21 days was significantly lower in the icodextrin group than in the sodium chloride group (p = 0.038 and p = 0.028, respectively). No significant differences were observed for macrophages, lymphocytes, reperitonealisation or the expression of COX-2, C-myc or Catenin. Conclusions The intraperitoneal application of 4% icodextrin reduces Adhesion formation in comparison to sodium chloride. 4% icodextrin solution reduces the inflammatory and mesenchymal infiltrate in the wounded area, thus improving the ratio of mesothel cells to mesenchymal infiltrate. As demonstrated, icodextrin is able to ameliorate the local tissue response. Further experimental studies would be done to elaborate the impact on the early response of the adaptive immune system, which may then trigger the subsequent wound healing and tissue repair.
Setya Anton - One of the best experts on this subject based on the ideXlab platform.
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Differences in the Effect of Using Sterile Water for Irrigation and Normal Saline Washing to Peritoneal Adhesion Post-Laparotomy on White Rats
The Indonesian Biomedical Journal, 2017Co-Authors: Octaviani Octaviani, Agus Rahardjo, Untung Alifianto, Setya AntonAbstract:BACKGROUND: Peritoneal Adhesion is a side effect of abdominal surgery that often occurs. Many efforts were made in abdominal surgery to prevent or minimize the occurrence of this Adhesion. One way to reduce the risk of bowel Adhesions is through washing Peritoneum method, so the differences in the use of washing solution on the incidence of Adhesions after laparotomy surgery need to be investigated. The objective of this study is to determine differences in the effect of using sterile water and normal saline for washing irrigation to peritoneal Adhesions after laparotomy in white rats. METHODS: This was an experimental study. Thirty-two sample of white rats were divided into two groups, group A and Group B, each group consists of 16 rats. Group A got washing using normal saline and group B got washing using sterile water for irrigation in laparotomy procedure to assess the peritoneal Adhesion microscopically after relaparotomy. RESULTS: This study was conducted in April-May 2016. The founding of peritoneal Adhesion after laparotomy in white rats using sterile water for irrigation in grade 1 were seven samples, grade 2 were five samples and grade 3 were four samples. Meanwhile, the founding of Peritoneum Adhesion after laparotomy using normal saline grade 1 are two samples, grade 2 are five samples, and grade 3 are nine samples. Statistically, significant differences were found ( p
Anton S. - One of the best experts on this subject based on the ideXlab platform.
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Differences in the Effect of Using Sterile Water for Irrigation and Normal Saline Washing to Peritoneal Adhesion Post-Laparotomy on White Rats
Prodia Education and Research Institute, 2017Co-Authors: Octaviani O., Rahardjo A., Alifianto U., Anton S.Abstract:BACKGROUND: Peritoneal Adhesion is a side effect of abdominal surgery that often occurs. Many efforts were made in abdominal surgery to prevent or minimize the occurrence of this Adhesion. One way to reduce the risk of bowel Adhesions is through washing Peritoneum method, so the differences in the use of washing solution on the incidence of Adhesions after laparotomy surgery need to be investigated. The objective of this study is to determine differences in the effect of using sterile water and normal saline for washing irrigation to peritoneal Adhesions after laparotomy in white rats.METHODS: This was an experimental study. Thirty-two sample of white rats were divided into two groups, group A and Group B, each group consists of 16 rats. Group A got washing using normal saline and group B got washing using sterile water for irrigation in laparotomy procedure to assess the peritoneal Adhesion microscopically after relaparotomy. Peritoneal Adhesion is one of the side effects that often occur after abdominal surgery.(1) Most of these conditions don\u27t cause problems, but about of 5% of peritoneal Adhesion cancause problems such as small bowel obstruction (SBO), infertility, pelvic and abdominal pain. These problems canRESULTS: This study was cunducted in April-May 2016. The founding of peritoneal Adhesion after laparotomy in white rats using sterile water for irrigation in grade 1 were seven samples, grade 2 were five samples and grade 3 were four samples. Meanwhile, the founding of Peritoneum Adhesion after laparotomy using normal saline grade 1 are two samples, grade 2 are five samples, and grade 3 are nine samples. Statistically, significant differences were found (p
Shin Yazawa - One of the best experts on this subject based on the ideXlab platform.
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Fucosyltransferases of the Peritoneum contributed to the Adhesion of cancer cells to the mesothelium
Cancer, 1995Co-Authors: Takayuki Asao, F.a.c.s. Yukio Nagamachi, Nobuhiro Morinaga, Yoshinori Shitara, Shei-ichi Takenoshita, Shin YazawaAbstract:Adhesion molecules associating with peritoneal dissemination were investigated using human gastric (MKN45 and MKN74) and colon (KM12C and KM12SM) cancer cells and the mouse Peritoneum. Adhesion of cancer cells to the Peritoneum was determined by a recently reported novel ex vivo method. MKN45 cells established from poorly differentiated adenocarcinoma with less glycosylated sugar chains on their cell surface showed higher Adhesion activities to the Peritoneum ex vivo and produced large amount of metastases in the abdominal cavity of nude mice, whereas MKN74 cells from differentiated adenocarcinoma with more glycosylated sugar chains showed slightly low Adhesion activity. KM12SM cells with highly metastatic potential to liver showed fairly low Adhesion activity to the Peritoneum compared with KM12C cells. The mouse Peritoneum was found to contain α1→2, α1→3, and α1→4 fucosyltransferases, and Adhesion of cancer cells was observed to the cellulose ester membrane, on which partially purified α-fucosyltransferases from mouse Peritoneum were immobilized. The Adhesion of cancer cells to fucosyltransferase-immobilized membrane was specifically inhibited by the addition of oligosaccharides and glycoproteins, which could serve as substrates for α-fucosyltransferases. These results indicate the contribution of α-fucosyltransferases to the Adhesion of disseminated cancer cells to the Peritoneum and support the possibility of antiAdhesion therapy of peritoneal dissemination by treatment with substrates for α-fucosyltransferases. Cancer 1995 ; 75 :1539-44.
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Fucosyltransferase of the Peritoneum contributed to the Adhesion of cancer cells to the mesothelium.
Cancer, 1995Co-Authors: Takayuki Asao, Nobuhiro Morinaga, Yoshinori Shitara, Shei-ichi Takenoshita, Y Nagamachi, Shin YazawaAbstract:Adhesion molecules associating with peritoneal dissemination were investigated using human gastric (MKN45 and MKN74) and colon (KM12C and KM12SM) cancer cells and the mouse Peritoneum. Adhesion of cancer cells to the Peritoneum was determined by a recently reported novel ex vivo method. MKN45 cells established from poorly differentiated adenocarcinoma with less glycosylated sugar chains on their cell surface showed higher Adhesion activities to the Peritoneum ex vivo and produced large amount of metastases in the abdominal cavity of nude mice, whereas MKN74 cells from differentiated adenocarcinoma with more glycosylated sugar chains showed slightly low Adhesion activity. KM12SM cells with highly metastatic potential to liver showed fairly low Adhesion activity to the Peritoneum compared with KM12C cells. The mouse Peritoneum was found to contain alpha 1 --> 2, alpha 1 --> 3, and alpha 1 --> 4 fucosyltransferases, and Adhesion of cancer cells was observed to the cellulose ester membrane, on which partially purified alpha-fucosyltransferases from mouse Peritoneum were immobilized. The Adhesion of cancer cells to fucosyltransferase-immobilized membrane was specifically inhibited by the addition of oligosaccharides and glycoproteins, which could serve as substrates for alpha-fucosyltransferases. These results indicate the contribution of alpha-fucosyltransferases to the Adhesion of disseminated cancer cells to the Peritoneum and support the possibility of antiAdhesion therapy of peritoneal dissemination by treatment with substrates for alpha-fucosyltransferases.