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Elizabeth F Rangel - One of the best experts on this subject based on the ideXlab platform.

  • first report of lutzomyia nyssomyia neivai diptera psychodidae phlebotominae naturally infected by leishmania viannia braziliensis in a Periurban Area of south brazil using a multiplex polymerase chain reaction assay
    American Journal of Tropical Medicine and Hygiene, 2009
    Co-Authors: Daniela De Pitapereira, Getulio D Souza, Adriana Zwetsch, Carlos Roberto Alves, Constanca Britto, Elizabeth F Rangel
    Abstract:

    To identify Lutzomyia (Nyssomyia) neivai naturally infected by Leishmania a multiplex polymerase chain reaction (PCR) was used for the analysis of 450 specimens (270 females, 180 males) collected in an endemic Periurban Area of cutaneous leishmaniasis in Porto Alegre, Brazil. Insects were grouped into pools of 10 and positive results were achieved in 3/27 Lu. (N.) neivai female pools. Infection by L. (Viannia) braziliensis was confirmed after hybridizing PCR products with a subgenus-specific biotinylated probe. Considering the detection of three positive pools with at least one infected insect in each, an infection rate of 1.1% was estimated. Our results associated with epidemiologic data suggest a potential ability of Lu. (N.) neivai in transmitting L. braziliensis in Porto Alegre, where the first notifications of autochthonous cutaneous leishmaniasis in humans occurred in 2002, with an increase in the number of cases in recent years possibly as a consequence of deforestation and agricultural activities in the Area.

  • first report of lutzomyia nyssomyia neivai diptera psychodidae phlebotominae naturally infected by leishmania viannia braziliensis in a Periurban Area of south brazil using a multiplex polymerase chain reaction assay
    American Journal of Tropical Medicine and Hygiene, 2009
    Co-Authors: Daniela De Pitapereira, Getulio D Souza, Adriana Zwetsch, Carlos Roberto Alves, Constanca Britto, Elizabeth F Rangel
    Abstract:

    To identify Lutzomyia ( Nyssomyia ) neivai naturally infected by Leishmania a multiplex polymerase chain reaction (PCR) was used for the analysis of 450 specimens (270 females, 180 males) collected in an endemic Periurban Area of cutaneous leishmaniasis in Porto Alegre, Brazil. Insects were grouped into pools of 10 and positive results were achieved in 3/27 Lu . ( N.) neivai female pools. Infection by L. ( Viannia ) braziliensis was confirmed after hybridizing PCR products with a subgenus-specific biotinylated probe. Considering the detection of three positive pools with at least one infected insect in each, an infection rate of 1.1% was estimated. Our results associated with epidemiologic data suggest a potential ability of Lu . ( N.) neivai in transmitting L. braziliensis in Porto Alegre, where the first notifications of autochtho- nous cutaneous leishmaniasis in humans occurred in 2002, with an increase in the number of cases in recent years possibly as a consequence of deforestation and agricultural activities in the Area. In the present study, a multiplex polymerase chain reac- tion (PCR) assay associated to non-isotopic hybridization was used to evaluate the occurrence of Lu. ( N. ) neivai naturally infected by Leishmania parasites in an urban Area of tourism activity located in Porto Alegre, where human ACL cases were recently described. Sand flies were collected monthly from October 2006 to May 2007 with Centers for Disease Control and Prevention (CDC) light traps in 10 monitoring stations distributed inside houses, in the peridomicile close to domestic animal shelters and in the forest. A sample constituted of 450 Lu. ( N.) neivai phlebotomines (270 females, 180 males) was

Daniela De Pitapereira - One of the best experts on this subject based on the ideXlab platform.

  • first report of lutzomyia nyssomyia neivai diptera psychodidae phlebotominae naturally infected by leishmania viannia braziliensis in a Periurban Area of south brazil using a multiplex polymerase chain reaction assay
    American Journal of Tropical Medicine and Hygiene, 2009
    Co-Authors: Daniela De Pitapereira, Getulio D Souza, Adriana Zwetsch, Carlos Roberto Alves, Constanca Britto, Elizabeth F Rangel
    Abstract:

    To identify Lutzomyia (Nyssomyia) neivai naturally infected by Leishmania a multiplex polymerase chain reaction (PCR) was used for the analysis of 450 specimens (270 females, 180 males) collected in an endemic Periurban Area of cutaneous leishmaniasis in Porto Alegre, Brazil. Insects were grouped into pools of 10 and positive results were achieved in 3/27 Lu. (N.) neivai female pools. Infection by L. (Viannia) braziliensis was confirmed after hybridizing PCR products with a subgenus-specific biotinylated probe. Considering the detection of three positive pools with at least one infected insect in each, an infection rate of 1.1% was estimated. Our results associated with epidemiologic data suggest a potential ability of Lu. (N.) neivai in transmitting L. braziliensis in Porto Alegre, where the first notifications of autochthonous cutaneous leishmaniasis in humans occurred in 2002, with an increase in the number of cases in recent years possibly as a consequence of deforestation and agricultural activities in the Area.

  • first report of lutzomyia nyssomyia neivai diptera psychodidae phlebotominae naturally infected by leishmania viannia braziliensis in a Periurban Area of south brazil using a multiplex polymerase chain reaction assay
    American Journal of Tropical Medicine and Hygiene, 2009
    Co-Authors: Daniela De Pitapereira, Getulio D Souza, Adriana Zwetsch, Carlos Roberto Alves, Constanca Britto, Elizabeth F Rangel
    Abstract:

    To identify Lutzomyia ( Nyssomyia ) neivai naturally infected by Leishmania a multiplex polymerase chain reaction (PCR) was used for the analysis of 450 specimens (270 females, 180 males) collected in an endemic Periurban Area of cutaneous leishmaniasis in Porto Alegre, Brazil. Insects were grouped into pools of 10 and positive results were achieved in 3/27 Lu . ( N.) neivai female pools. Infection by L. ( Viannia ) braziliensis was confirmed after hybridizing PCR products with a subgenus-specific biotinylated probe. Considering the detection of three positive pools with at least one infected insect in each, an infection rate of 1.1% was estimated. Our results associated with epidemiologic data suggest a potential ability of Lu . ( N.) neivai in transmitting L. braziliensis in Porto Alegre, where the first notifications of autochtho- nous cutaneous leishmaniasis in humans occurred in 2002, with an increase in the number of cases in recent years possibly as a consequence of deforestation and agricultural activities in the Area. In the present study, a multiplex polymerase chain reac- tion (PCR) assay associated to non-isotopic hybridization was used to evaluate the occurrence of Lu. ( N. ) neivai naturally infected by Leishmania parasites in an urban Area of tourism activity located in Porto Alegre, where human ACL cases were recently described. Sand flies were collected monthly from October 2006 to May 2007 with Centers for Disease Control and Prevention (CDC) light traps in 10 monitoring stations distributed inside houses, in the peridomicile close to domestic animal shelters and in the forest. A sample constituted of 450 Lu. ( N.) neivai phlebotomines (270 females, 180 males) was

Getulio D Souza - One of the best experts on this subject based on the ideXlab platform.

  • first report of lutzomyia nyssomyia neivai diptera psychodidae phlebotominae naturally infected by leishmania viannia braziliensis in a Periurban Area of south brazil using a multiplex polymerase chain reaction assay
    American Journal of Tropical Medicine and Hygiene, 2009
    Co-Authors: Daniela De Pitapereira, Getulio D Souza, Adriana Zwetsch, Carlos Roberto Alves, Constanca Britto, Elizabeth F Rangel
    Abstract:

    To identify Lutzomyia (Nyssomyia) neivai naturally infected by Leishmania a multiplex polymerase chain reaction (PCR) was used for the analysis of 450 specimens (270 females, 180 males) collected in an endemic Periurban Area of cutaneous leishmaniasis in Porto Alegre, Brazil. Insects were grouped into pools of 10 and positive results were achieved in 3/27 Lu. (N.) neivai female pools. Infection by L. (Viannia) braziliensis was confirmed after hybridizing PCR products with a subgenus-specific biotinylated probe. Considering the detection of three positive pools with at least one infected insect in each, an infection rate of 1.1% was estimated. Our results associated with epidemiologic data suggest a potential ability of Lu. (N.) neivai in transmitting L. braziliensis in Porto Alegre, where the first notifications of autochthonous cutaneous leishmaniasis in humans occurred in 2002, with an increase in the number of cases in recent years possibly as a consequence of deforestation and agricultural activities in the Area.

  • first report of lutzomyia nyssomyia neivai diptera psychodidae phlebotominae naturally infected by leishmania viannia braziliensis in a Periurban Area of south brazil using a multiplex polymerase chain reaction assay
    American Journal of Tropical Medicine and Hygiene, 2009
    Co-Authors: Daniela De Pitapereira, Getulio D Souza, Adriana Zwetsch, Carlos Roberto Alves, Constanca Britto, Elizabeth F Rangel
    Abstract:

    To identify Lutzomyia ( Nyssomyia ) neivai naturally infected by Leishmania a multiplex polymerase chain reaction (PCR) was used for the analysis of 450 specimens (270 females, 180 males) collected in an endemic Periurban Area of cutaneous leishmaniasis in Porto Alegre, Brazil. Insects were grouped into pools of 10 and positive results were achieved in 3/27 Lu . ( N.) neivai female pools. Infection by L. ( Viannia ) braziliensis was confirmed after hybridizing PCR products with a subgenus-specific biotinylated probe. Considering the detection of three positive pools with at least one infected insect in each, an infection rate of 1.1% was estimated. Our results associated with epidemiologic data suggest a potential ability of Lu . ( N.) neivai in transmitting L. braziliensis in Porto Alegre, where the first notifications of autochtho- nous cutaneous leishmaniasis in humans occurred in 2002, with an increase in the number of cases in recent years possibly as a consequence of deforestation and agricultural activities in the Area. In the present study, a multiplex polymerase chain reac- tion (PCR) assay associated to non-isotopic hybridization was used to evaluate the occurrence of Lu. ( N. ) neivai naturally infected by Leishmania parasites in an urban Area of tourism activity located in Porto Alegre, where human ACL cases were recently described. Sand flies were collected monthly from October 2006 to May 2007 with Centers for Disease Control and Prevention (CDC) light traps in 10 monitoring stations distributed inside houses, in the peridomicile close to domestic animal shelters and in the forest. A sample constituted of 450 Lu. ( N.) neivai phlebotomines (270 females, 180 males) was

Adriana Zwetsch - One of the best experts on this subject based on the ideXlab platform.

  • first report of lutzomyia nyssomyia neivai diptera psychodidae phlebotominae naturally infected by leishmania viannia braziliensis in a Periurban Area of south brazil using a multiplex polymerase chain reaction assay
    American Journal of Tropical Medicine and Hygiene, 2009
    Co-Authors: Daniela De Pitapereira, Getulio D Souza, Adriana Zwetsch, Carlos Roberto Alves, Constanca Britto, Elizabeth F Rangel
    Abstract:

    To identify Lutzomyia (Nyssomyia) neivai naturally infected by Leishmania a multiplex polymerase chain reaction (PCR) was used for the analysis of 450 specimens (270 females, 180 males) collected in an endemic Periurban Area of cutaneous leishmaniasis in Porto Alegre, Brazil. Insects were grouped into pools of 10 and positive results were achieved in 3/27 Lu. (N.) neivai female pools. Infection by L. (Viannia) braziliensis was confirmed after hybridizing PCR products with a subgenus-specific biotinylated probe. Considering the detection of three positive pools with at least one infected insect in each, an infection rate of 1.1% was estimated. Our results associated with epidemiologic data suggest a potential ability of Lu. (N.) neivai in transmitting L. braziliensis in Porto Alegre, where the first notifications of autochthonous cutaneous leishmaniasis in humans occurred in 2002, with an increase in the number of cases in recent years possibly as a consequence of deforestation and agricultural activities in the Area.

  • first report of lutzomyia nyssomyia neivai diptera psychodidae phlebotominae naturally infected by leishmania viannia braziliensis in a Periurban Area of south brazil using a multiplex polymerase chain reaction assay
    American Journal of Tropical Medicine and Hygiene, 2009
    Co-Authors: Daniela De Pitapereira, Getulio D Souza, Adriana Zwetsch, Carlos Roberto Alves, Constanca Britto, Elizabeth F Rangel
    Abstract:

    To identify Lutzomyia ( Nyssomyia ) neivai naturally infected by Leishmania a multiplex polymerase chain reaction (PCR) was used for the analysis of 450 specimens (270 females, 180 males) collected in an endemic Periurban Area of cutaneous leishmaniasis in Porto Alegre, Brazil. Insects were grouped into pools of 10 and positive results were achieved in 3/27 Lu . ( N.) neivai female pools. Infection by L. ( Viannia ) braziliensis was confirmed after hybridizing PCR products with a subgenus-specific biotinylated probe. Considering the detection of three positive pools with at least one infected insect in each, an infection rate of 1.1% was estimated. Our results associated with epidemiologic data suggest a potential ability of Lu . ( N.) neivai in transmitting L. braziliensis in Porto Alegre, where the first notifications of autochtho- nous cutaneous leishmaniasis in humans occurred in 2002, with an increase in the number of cases in recent years possibly as a consequence of deforestation and agricultural activities in the Area. In the present study, a multiplex polymerase chain reac- tion (PCR) assay associated to non-isotopic hybridization was used to evaluate the occurrence of Lu. ( N. ) neivai naturally infected by Leishmania parasites in an urban Area of tourism activity located in Porto Alegre, where human ACL cases were recently described. Sand flies were collected monthly from October 2006 to May 2007 with Centers for Disease Control and Prevention (CDC) light traps in 10 monitoring stations distributed inside houses, in the peridomicile close to domestic animal shelters and in the forest. A sample constituted of 450 Lu. ( N.) neivai phlebotomines (270 females, 180 males) was

Carlos Roberto Alves - One of the best experts on this subject based on the ideXlab platform.

  • first report of lutzomyia nyssomyia neivai diptera psychodidae phlebotominae naturally infected by leishmania viannia braziliensis in a Periurban Area of south brazil using a multiplex polymerase chain reaction assay
    American Journal of Tropical Medicine and Hygiene, 2009
    Co-Authors: Daniela De Pitapereira, Getulio D Souza, Adriana Zwetsch, Carlos Roberto Alves, Constanca Britto, Elizabeth F Rangel
    Abstract:

    To identify Lutzomyia (Nyssomyia) neivai naturally infected by Leishmania a multiplex polymerase chain reaction (PCR) was used for the analysis of 450 specimens (270 females, 180 males) collected in an endemic Periurban Area of cutaneous leishmaniasis in Porto Alegre, Brazil. Insects were grouped into pools of 10 and positive results were achieved in 3/27 Lu. (N.) neivai female pools. Infection by L. (Viannia) braziliensis was confirmed after hybridizing PCR products with a subgenus-specific biotinylated probe. Considering the detection of three positive pools with at least one infected insect in each, an infection rate of 1.1% was estimated. Our results associated with epidemiologic data suggest a potential ability of Lu. (N.) neivai in transmitting L. braziliensis in Porto Alegre, where the first notifications of autochthonous cutaneous leishmaniasis in humans occurred in 2002, with an increase in the number of cases in recent years possibly as a consequence of deforestation and agricultural activities in the Area.

  • first report of lutzomyia nyssomyia neivai diptera psychodidae phlebotominae naturally infected by leishmania viannia braziliensis in a Periurban Area of south brazil using a multiplex polymerase chain reaction assay
    American Journal of Tropical Medicine and Hygiene, 2009
    Co-Authors: Daniela De Pitapereira, Getulio D Souza, Adriana Zwetsch, Carlos Roberto Alves, Constanca Britto, Elizabeth F Rangel
    Abstract:

    To identify Lutzomyia ( Nyssomyia ) neivai naturally infected by Leishmania a multiplex polymerase chain reaction (PCR) was used for the analysis of 450 specimens (270 females, 180 males) collected in an endemic Periurban Area of cutaneous leishmaniasis in Porto Alegre, Brazil. Insects were grouped into pools of 10 and positive results were achieved in 3/27 Lu . ( N.) neivai female pools. Infection by L. ( Viannia ) braziliensis was confirmed after hybridizing PCR products with a subgenus-specific biotinylated probe. Considering the detection of three positive pools with at least one infected insect in each, an infection rate of 1.1% was estimated. Our results associated with epidemiologic data suggest a potential ability of Lu . ( N.) neivai in transmitting L. braziliensis in Porto Alegre, where the first notifications of autochtho- nous cutaneous leishmaniasis in humans occurred in 2002, with an increase in the number of cases in recent years possibly as a consequence of deforestation and agricultural activities in the Area. In the present study, a multiplex polymerase chain reac- tion (PCR) assay associated to non-isotopic hybridization was used to evaluate the occurrence of Lu. ( N. ) neivai naturally infected by Leishmania parasites in an urban Area of tourism activity located in Porto Alegre, where human ACL cases were recently described. Sand flies were collected monthly from October 2006 to May 2007 with Centers for Disease Control and Prevention (CDC) light traps in 10 monitoring stations distributed inside houses, in the peridomicile close to domestic animal shelters and in the forest. A sample constituted of 450 Lu. ( N.) neivai phlebotomines (270 females, 180 males) was