The Experts below are selected from a list of 15 Experts worldwide ranked by ideXlab platform
Neda Slade - One of the best experts on this subject based on the ideXlab platform.
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immunostaining of frozen sections using Peroxidase antiPeroxidase Complex
2007Co-Authors: Neda SladeAbstract:Imunohistokemijsko bojenje može se koristiti i za ispitivanje ekspresije proteina na rezovima smrznutih tkiva.
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immunostaining of paraffin embeded tissue using Peroxidase antiPeroxidase Complex
2007Co-Authors: Neda SladeAbstract:Imunohistokemijsko bojenje važno je za ispitivanje ekspresije proteina u arhivskim tkivima uklopljenim u parafin.
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immunostaining of tissue culture cells using Peroxidase antiPeroxidase Complex
2007Co-Authors: Neda SladeAbstract:Imunocitokemijskim bojenjem može se odrediti smjestaj proteina unutar stanice, koje se mogu prethodno tretirati ili transfecirati.
Elizabeth H Hammond - One of the best experts on this subject based on the ideXlab platform.
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quantitative Peroxidase antiPeroxidase Complex substrate mass determination in tissue sections by a dual wavelength method
Analytical and Quantitative Cytology and Histology, 1992Co-Authors: R Zhou, Dennis L Parker, Elizabeth H HammondAbstract:The aim of this study was to develop a method of quantitating prostate-specific acid phosphatase (PSAP) in histologic sections of prostate tumor tissue labeled with the Peroxidase-antiPeroxidase (PAP) Complex technique using diaminobenzidine (DAB) as a substrate. Studies of PAP-DAB- and hematoxylin-stained prostate tissue sections were performed with a black-and-white, computerized microscope image system. The mass of brown reaction product generated in PAP-DAB staining was the indicator of PSAP intensity. The mass of brown reaction product was determined by using a dual wavelength method in which two 10-nm bandpass filters, peaked at 450 and 510 nm in wavelength, were used. The wavelength-dependent ratio of mass absorptivity of PAP-DAB stain (brown product) and that of hematoxylin (blue product) were estimated at wavelengths of 450 and 510 nm by using slides stained with only PAP-DAB or hematoxylin. The accuracy of the mass measurements, investigated by relating the measurement to the true mass of the brown PAP-DAB product, is reported. There was no significant difference between the measurements at magnifications of 10x, 20x, 40x or 60x in the reproducibility investigation. The PSAP stain intensity was quantitatively determined by the difference of the PAP-DAB stain mass per pixel between the tumor and normal cell region. The relationship between the objective measurement and the conventional subjective grades is presented.
George Weinbaum - One of the best experts on this subject based on the ideXlab platform.
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utilization of a Peroxidase antiPeroxidase Complex in an enzyme linked immunosorbent assay of elastin derived peptides in human plasma
The American review of respiratory disease, 2015Co-Authors: Umberto Kucich, Paul J Christner, Michael Lippmann, Philip Kimbel, Greg Williams, Joel Rosenbloom, George WeinbaumAbstract:Chronic obstructive pulmonary disease (COPD), a major cause of morbidity and death in the smoking population, develops insidiously over many years, and significant impairment of lung function usually occurs before the disease is diagnosed. Because lung elastin degradation appears to be a prerequisite for the development of the disease, immunologic detection of elastin-derived peptides in the blood might be an effective approach to the early detection and monitoring of the disease. We here report an improved enzyme-linked immunosorbent assay for elastin peptides using a Peroxidase-antiPeroxidase Complex as the reporter group. The assay is sensitive to 2 ng/ml elastin peptides. We show that for optimal, reproducible results the assay should be carried out at 16° C rather than at room temperature and that determinations should be made on plasma containing protease inhibitors rather than on serum. The levels of elastin-derived peptides appeared to remain relatively constant when multiple samples were taken du...
R Zhou - One of the best experts on this subject based on the ideXlab platform.
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quantitative Peroxidase antiPeroxidase Complex substrate mass determination in tissue sections by a dual wavelength method
Analytical and Quantitative Cytology and Histology, 1992Co-Authors: R Zhou, Dennis L Parker, Elizabeth H HammondAbstract:The aim of this study was to develop a method of quantitating prostate-specific acid phosphatase (PSAP) in histologic sections of prostate tumor tissue labeled with the Peroxidase-antiPeroxidase (PAP) Complex technique using diaminobenzidine (DAB) as a substrate. Studies of PAP-DAB- and hematoxylin-stained prostate tissue sections were performed with a black-and-white, computerized microscope image system. The mass of brown reaction product generated in PAP-DAB staining was the indicator of PSAP intensity. The mass of brown reaction product was determined by using a dual wavelength method in which two 10-nm bandpass filters, peaked at 450 and 510 nm in wavelength, were used. The wavelength-dependent ratio of mass absorptivity of PAP-DAB stain (brown product) and that of hematoxylin (blue product) were estimated at wavelengths of 450 and 510 nm by using slides stained with only PAP-DAB or hematoxylin. The accuracy of the mass measurements, investigated by relating the measurement to the true mass of the brown PAP-DAB product, is reported. There was no significant difference between the measurements at magnifications of 10x, 20x, 40x or 60x in the reproducibility investigation. The PSAP stain intensity was quantitatively determined by the difference of the PAP-DAB stain mass per pixel between the tumor and normal cell region. The relationship between the objective measurement and the conventional subjective grades is presented.
Umberto Kucich - One of the best experts on this subject based on the ideXlab platform.
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utilization of a Peroxidase antiPeroxidase Complex in an enzyme linked immunosorbent assay of elastin derived peptides in human plasma
The American review of respiratory disease, 2015Co-Authors: Umberto Kucich, Paul J Christner, Michael Lippmann, Philip Kimbel, Greg Williams, Joel Rosenbloom, George WeinbaumAbstract:Chronic obstructive pulmonary disease (COPD), a major cause of morbidity and death in the smoking population, develops insidiously over many years, and significant impairment of lung function usually occurs before the disease is diagnosed. Because lung elastin degradation appears to be a prerequisite for the development of the disease, immunologic detection of elastin-derived peptides in the blood might be an effective approach to the early detection and monitoring of the disease. We here report an improved enzyme-linked immunosorbent assay for elastin peptides using a Peroxidase-antiPeroxidase Complex as the reporter group. The assay is sensitive to 2 ng/ml elastin peptides. We show that for optimal, reproducible results the assay should be carried out at 16° C rather than at room temperature and that determinations should be made on plasma containing protease inhibitors rather than on serum. The levels of elastin-derived peptides appeared to remain relatively constant when multiple samples were taken du...