The Experts below are selected from a list of 360 Experts worldwide ranked by ideXlab platform
Yongsheng Che - One of the best experts on this subject based on the ideXlab platform.
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genetic manipulation of the cop9 signalosome subunit pfcsne leads to the discovery of pestaloficins in Pestalotiopsis fici
Organic Letters, 2017Co-Authors: Yanjing Zheng, Haining Lyu, Ying Huang, Hongwei Liu, Ling Liu, Yongsheng Che, Xingzhong Liu, Huixi Zou, Wen-bing YinAbstract:By deleting the COP9 signalosome subunit PfcsnE from Pestalotiopsis fici, seven compounds that were newly produced by the mutant could be characterized, including five new structures, pestaloficins A–E (1 and 3–6). Pestaloficin A (1) represents a new type of dimeric cyclohexanone derivative linked through an unprecedented pentacyclic spiral ring.
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Genetic Manipulation of the COP9 Signalosome Subunit PfCsnE Leads to the Discovery of Pestaloficins in Pestalotiopsis fici
2017Co-Authors: Yanjing Zheng, Haining Lyu, Ying Huang, Hongwei Liu, Ling Liu, Yongsheng Che, Xingzhong Liu, Huixi Zou, Wen-bing YinAbstract:By deleting the COP9 signalosome subunit PfcsnE from Pestalotiopsis fici, seven compounds that were newly produced by the mutant could be characterized, including five new structures, pestaloficins A–E (1 and 3–6). Pestaloficin A (1) represents a new type of dimeric cyclohexanone derivative linked through an unprecedented pentacyclic spiral ring
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pestalotriols a and b new spiro 2 5 octane derivatives from the endophytic fungus Pestalotiopsis fici
ChemInform, 2016Co-Authors: Ling Liu, Liang-dong Guo, Chen Zhao, Yongsheng CheAbstract:Pestalotriols A (1) and B (2), two new metabolites featuring a unique spiro[2.5]octane skeleton, were isolated from an endophytic fungus Pestalotiopsis fici. Their structures were elucidated primarily by NMR experiments, and the absolute configurations of 1 and 2 were assigned by electronic circular dichroism calculations.
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genomic and transcriptomic analysis of the endophytic fungus Pestalotiopsis fici reveals its lifestyle and high potential for synthesis of natural products
BMC Genomics, 2015Co-Authors: Ling Liu, Yongsheng Che, Liang-dong Guo, Xiang Sun, Xiuna Wang, Xiaoling Zhang, Meichun Xiang, W Wang, Gang LiuAbstract:Background: In recent years, the genus Pestalotiopsis is receiving increasing attention, not only because of its economic impact as a plant pathogen but also as a commonly isolated endophyte which is an important source of bioactive natural products. Pestalotiopsis fici Steyaert W106-1/CGMCC3.15140 as an endophyte of tea produces numerous novel secondary metabolites, including chloropupukeananin, a derivative of chlorinated pupukeanane that is first discovered in fungi. Some of them might be important as the drug leads for future pharmaceutics. Results: Here, we report the genome sequence of the endophytic fungus of tea Pestalotiopsis fici W106-1/CGMCC3.15140. The abundant carbohydrate-active enzymes especially significantly expanding pectinases allow the fungus to utilize the limited intercellular nutrients within the host plants, suggesting adaptation of the fungus to endophytic lifestyle. The P. fici genome encodes a rich set of secondary metabolite synthesis genes, including 27 polyketide synthases (PKSs), 12 non-ribosomal peptide synthases (NRPSs), five dimethylallyl tryptophan synthases, four putative PKS-like enzymes, 15 putative NRPS-like enzymes, 15 terpenoid synthases, seven terpenoid cyclases, seven fatty-acid synthases, and five hybrids of PKS-NRPS. The majority of these core enzymes distributed into 74 secondary metabolite clusters. The putative Diels-Alderase genes have undergone expansion. Conclusion: The significant expansion of pectinase encoding genes provides essential insight in the life strategy of endophytes, and richness of gene clusters for secondary metabolites reveals high potential of natural products of endophytic fungi.
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genomic and transcriptomic analysis of the endophytic fungus Pestalotiopsis fici reveals its lifestyle and high potential for synthesis of natural products
BMC Genomics, 2015Co-Authors: Ling Liu, Yongsheng Che, Liang-dong Guo, Xiang Sun, Xiuna Wang, Xiaoling Zhang, Meichun Xiang, W Wang, Gang LiuAbstract:In recent years, the genus Pestalotiopsis is receiving increasing attention, not only because of its economic impact as a plant pathogen but also as a commonly isolated endophyte which is an important source of bioactive natural products. Pestalotiopsis fici Steyaert W106-1/CGMCC3.15140 as an endophyte of tea produces numerous novel secondary metabolites, including chloropupukeananin, a derivative of chlorinated pupukeanane that is first discovered in fungi. Some of them might be important as the drug leads for future pharmaceutics. Here, we report the genome sequence of the endophytic fungus of tea Pestalotiopsis fici W106-1/CGMCC3.15140. The abundant carbohydrate-active enzymes especially significantly expanding pectinases allow the fungus to utilize the limited intercellular nutrients within the host plants, suggesting adaptation of the fungus to endophytic lifestyle. The P. fici genome encodes a rich set of secondary metabolite synthesis genes, including 27 polyketide synthases (PKSs), 12 non-ribosomal peptide synthases (NRPSs), five dimethylallyl tryptophan synthases, four putative PKS-like enzymes, 15 putative NRPS-like enzymes, 15 terpenoid synthases, seven terpenoid cyclases, seven fatty-acid synthases, and five hybrids of PKS-NRPS. The majority of these core enzymes distributed into 74 secondary metabolite clusters. The putative Diels-Alderase genes have undergone expansion. The significant expansion of pectinase encoding genes provides essential insight in the life strategy of endophytes, and richness of gene clusters for secondary metabolites reveals high potential of natural products of endophytic fungi.
Liang-dong Guo - One of the best experts on this subject based on the ideXlab platform.
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pestalotriols a and b new spiro 2 5 octane derivatives from the endophytic fungus Pestalotiopsis fici
ChemInform, 2016Co-Authors: Ling Liu, Liang-dong Guo, Chen Zhao, Yongsheng CheAbstract:Pestalotriols A (1) and B (2), two new metabolites featuring a unique spiro[2.5]octane skeleton, were isolated from an endophytic fungus Pestalotiopsis fici. Their structures were elucidated primarily by NMR experiments, and the absolute configurations of 1 and 2 were assigned by electronic circular dichroism calculations.
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microsporols a c from the plant endophytic fungus Pestalotiopsis microspore
Natural Product Communications, 2015Co-Authors: Yadan Wang, Shuchun Liu, Xinzhong Liu, Liang-dong GuoAbstract:Three new ambuic acid derivatives, microsporols A-C (1-3) and the known compound ambuic acid (4), were isolated from the solid-substrate fermentation cultures of the plant endophytic fungus Pestalotiopsis microspora. Their structures were elucidated primarily by NMR experiments. The absolute configurations of the 6,7-diol moiety in 1 and 2 were assigned using the Snatzke's method, whereas that of 3 was deduced by circular dichroism (CD) exciton chirality method. Compounds 1, 3, and 4 showed moderate 5-lipoxygenase (5-LOX) inhibitory effects.
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genomic and transcriptomic analysis of the endophytic fungus Pestalotiopsis fici reveals its lifestyle and high potential for synthesis of natural products
BMC Genomics, 2015Co-Authors: Ling Liu, Yongsheng Che, Liang-dong Guo, Xiang Sun, Xiuna Wang, Xiaoling Zhang, Meichun Xiang, W Wang, Gang LiuAbstract:Background: In recent years, the genus Pestalotiopsis is receiving increasing attention, not only because of its economic impact as a plant pathogen but also as a commonly isolated endophyte which is an important source of bioactive natural products. Pestalotiopsis fici Steyaert W106-1/CGMCC3.15140 as an endophyte of tea produces numerous novel secondary metabolites, including chloropupukeananin, a derivative of chlorinated pupukeanane that is first discovered in fungi. Some of them might be important as the drug leads for future pharmaceutics. Results: Here, we report the genome sequence of the endophytic fungus of tea Pestalotiopsis fici W106-1/CGMCC3.15140. The abundant carbohydrate-active enzymes especially significantly expanding pectinases allow the fungus to utilize the limited intercellular nutrients within the host plants, suggesting adaptation of the fungus to endophytic lifestyle. The P. fici genome encodes a rich set of secondary metabolite synthesis genes, including 27 polyketide synthases (PKSs), 12 non-ribosomal peptide synthases (NRPSs), five dimethylallyl tryptophan synthases, four putative PKS-like enzymes, 15 putative NRPS-like enzymes, 15 terpenoid synthases, seven terpenoid cyclases, seven fatty-acid synthases, and five hybrids of PKS-NRPS. The majority of these core enzymes distributed into 74 secondary metabolite clusters. The putative Diels-Alderase genes have undergone expansion. Conclusion: The significant expansion of pectinase encoding genes provides essential insight in the life strategy of endophytes, and richness of gene clusters for secondary metabolites reveals high potential of natural products of endophytic fungi.
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genomic and transcriptomic analysis of the endophytic fungus Pestalotiopsis fici reveals its lifestyle and high potential for synthesis of natural products
BMC Genomics, 2015Co-Authors: Ling Liu, Yongsheng Che, Liang-dong Guo, Xiang Sun, Xiuna Wang, Xiaoling Zhang, Meichun Xiang, W Wang, Gang LiuAbstract:In recent years, the genus Pestalotiopsis is receiving increasing attention, not only because of its economic impact as a plant pathogen but also as a commonly isolated endophyte which is an important source of bioactive natural products. Pestalotiopsis fici Steyaert W106-1/CGMCC3.15140 as an endophyte of tea produces numerous novel secondary metabolites, including chloropupukeananin, a derivative of chlorinated pupukeanane that is first discovered in fungi. Some of them might be important as the drug leads for future pharmaceutics. Here, we report the genome sequence of the endophytic fungus of tea Pestalotiopsis fici W106-1/CGMCC3.15140. The abundant carbohydrate-active enzymes especially significantly expanding pectinases allow the fungus to utilize the limited intercellular nutrients within the host plants, suggesting adaptation of the fungus to endophytic lifestyle. The P. fici genome encodes a rich set of secondary metabolite synthesis genes, including 27 polyketide synthases (PKSs), 12 non-ribosomal peptide synthases (NRPSs), five dimethylallyl tryptophan synthases, four putative PKS-like enzymes, 15 putative NRPS-like enzymes, 15 terpenoid synthases, seven terpenoid cyclases, seven fatty-acid synthases, and five hybrids of PKS-NRPS. The majority of these core enzymes distributed into 74 secondary metabolite clusters. The putative Diels-Alderase genes have undergone expansion. The significant expansion of pectinase encoding genes provides essential insight in the life strategy of endophytes, and richness of gene clusters for secondary metabolites reveals high potential of natural products of endophytic fungi.
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improving the backbone tree for the genus Pestalotiopsis addition of p steyaertii and p magna sp nov
Mycological Progress, 2014Co-Authors: Liang-dong Guo, Sajeewa S N Maharachchikumbura, Ekachai Chukeatirote, Kevin D. HydeAbstract:A novel, saprobic Pestalotiopsis species isolated from the decaying leaves of Pteridium sp. collected in France is described as Pestalotiopsis magna. The novelty of the species is confirmed based on phenotypic analyses of conidial characters and phylogenetic analyses of sequence data. Pestalotiopsis magna can also be distinguished from similar and related species by its larger conidia. Phylogenetic species recognition, based on combined, multilocus alignment of the internal transcribed spacer (ITS), partial β-tubulin, and partial translation elongation factor 1-alpha (tef1), strongly supported the monophyly of P. magna with relation to other versicolorous species. The ex-type culture of P. steyaertii was also sequenced and placed in the backbone tree for Pestalotiopsis.
Ling Liu - One of the best experts on this subject based on the ideXlab platform.
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caryophyllene type sesquiterpenoids and α furanones from the plant endophytic fungus Pestalotiopsis theae
Chinese Journal of Natural Medicines, 2020Co-Authors: Longfang Guo, Gaoran Liu, Ling LiuAbstract:Two new caryophyllene-type sesquiterpenoids, pestathenols A (1) and B (2) and one new α-furanone, pestatheranone A (6), along with five known compounds (3-5, 7 and 8) have been isolated from the crude extract of the plant endophytic fungus Pestalotiopsis theae. Their structures were unambiguously established by extensive spectroscopic analyses. The absolute configuration of the 5,6-diol moiety in 1 was assigned using Snatzke's method. Compounds 1 and 2 showed weak cytotoxicity against HeLa cell line.
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genetic manipulation of the cop9 signalosome subunit pfcsne leads to the discovery of pestaloficins in Pestalotiopsis fici
Organic Letters, 2017Co-Authors: Yanjing Zheng, Haining Lyu, Ying Huang, Hongwei Liu, Ling Liu, Yongsheng Che, Xingzhong Liu, Huixi Zou, Wen-bing YinAbstract:By deleting the COP9 signalosome subunit PfcsnE from Pestalotiopsis fici, seven compounds that were newly produced by the mutant could be characterized, including five new structures, pestaloficins A–E (1 and 3–6). Pestaloficin A (1) represents a new type of dimeric cyclohexanone derivative linked through an unprecedented pentacyclic spiral ring.
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Genetic Manipulation of the COP9 Signalosome Subunit PfCsnE Leads to the Discovery of Pestaloficins in Pestalotiopsis fici
2017Co-Authors: Yanjing Zheng, Haining Lyu, Ying Huang, Hongwei Liu, Ling Liu, Yongsheng Che, Xingzhong Liu, Huixi Zou, Wen-bing YinAbstract:By deleting the COP9 signalosome subunit PfcsnE from Pestalotiopsis fici, seven compounds that were newly produced by the mutant could be characterized, including five new structures, pestaloficins A–E (1 and 3–6). Pestaloficin A (1) represents a new type of dimeric cyclohexanone derivative linked through an unprecedented pentacyclic spiral ring
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pestalotriols a and b new spiro 2 5 octane derivatives from the endophytic fungus Pestalotiopsis fici
ChemInform, 2016Co-Authors: Ling Liu, Liang-dong Guo, Chen Zhao, Yongsheng CheAbstract:Pestalotriols A (1) and B (2), two new metabolites featuring a unique spiro[2.5]octane skeleton, were isolated from an endophytic fungus Pestalotiopsis fici. Their structures were elucidated primarily by NMR experiments, and the absolute configurations of 1 and 2 were assigned by electronic circular dichroism calculations.
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genomic and transcriptomic analysis of the endophytic fungus Pestalotiopsis fici reveals its lifestyle and high potential for synthesis of natural products
BMC Genomics, 2015Co-Authors: Ling Liu, Yongsheng Che, Liang-dong Guo, Xiang Sun, Xiuna Wang, Xiaoling Zhang, Meichun Xiang, W Wang, Gang LiuAbstract:Background: In recent years, the genus Pestalotiopsis is receiving increasing attention, not only because of its economic impact as a plant pathogen but also as a commonly isolated endophyte which is an important source of bioactive natural products. Pestalotiopsis fici Steyaert W106-1/CGMCC3.15140 as an endophyte of tea produces numerous novel secondary metabolites, including chloropupukeananin, a derivative of chlorinated pupukeanane that is first discovered in fungi. Some of them might be important as the drug leads for future pharmaceutics. Results: Here, we report the genome sequence of the endophytic fungus of tea Pestalotiopsis fici W106-1/CGMCC3.15140. The abundant carbohydrate-active enzymes especially significantly expanding pectinases allow the fungus to utilize the limited intercellular nutrients within the host plants, suggesting adaptation of the fungus to endophytic lifestyle. The P. fici genome encodes a rich set of secondary metabolite synthesis genes, including 27 polyketide synthases (PKSs), 12 non-ribosomal peptide synthases (NRPSs), five dimethylallyl tryptophan synthases, four putative PKS-like enzymes, 15 putative NRPS-like enzymes, 15 terpenoid synthases, seven terpenoid cyclases, seven fatty-acid synthases, and five hybrids of PKS-NRPS. The majority of these core enzymes distributed into 74 secondary metabolite clusters. The putative Diels-Alderase genes have undergone expansion. Conclusion: The significant expansion of pectinase encoding genes provides essential insight in the life strategy of endophytes, and richness of gene clusters for secondary metabolites reveals high potential of natural products of endophytic fungi.
Peter Proksch - One of the best experts on this subject based on the ideXlab platform.
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Pestalotiopsis a highly creative genus chemistry and bioactivity of secondary metabolites
Fungal Diversity, 2010Co-Authors: Sherif S Ebada, Peter ProkschAbstract:Fungal endophytes are receiving increasing attention by natural product chemists due to their diverse and structurally unprecedented compounds which make them interesting candidates for drug discovery. One fungal genus which is especially productive with regard to the accumulation of a diverse array of mostly bioactive compounds is Pestalotiopsis. This review demonstrates the unique chemical diversity of taxa from this genus and describes their reported bioactivities. To date, alkaloids, terpenoids, isocoumarin derivatives, coumarins, chromones, quinones, semiquinones, peptides, xanthones, xanthone derivatives, phenols, phenolic acids, and lactones, have been reported from Pestalotiopsis species, making this genus a particularly rich source for bioprospecting when compared with other fungal genera. More than 130 different compounds that have been isolated from various species of Pestalotiopsis are covered in this review and their structures are reported within a biogenetic context. Bioassays disclosed that antifungal, antimicrobial, and antitumor activities are the most notable bioactivities of secondary metabolites isolated from this genus. Possible biogenetic pathways for monoterpenes, sesquiterpenes, quinones, and lactones that are covered in this review article are also illustrated.
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cytosporones coumarins and an alkaloid from the endophytic fungus Pestalotiopsis sp isolated from the chinese mangrove plant rhizophora mucronata
Bioorganic & Medicinal Chemistry, 2009Co-Authors: Julia Kjer, Jandirk Sendker, Victor Wray, Huashi Guan, Ruangelie Edrada, Werner E G Muller, Mirko Bayer, Wenhan Lin, Peter ProkschAbstract:Chemical examination of the endophytic fungus Pestalotiopsis sp., isolated from the leaves of the Chinese mangrove Rhizophora mucronata, yielded 11 new compounds including cytosporones J-N (1-3, 5-6), five new coumarins pestalasins A-E (8-12), and a new alkaloid named pestalotiopsoid A (14), along with the known compounds cytosporone C (4), dothiorelone B (7), and 3-hydroxymethyl-6,8-dimethoxycoumarin (13). The structures of the new compounds were unambiguously elucidated on the basis of extensive spectroscopic data analysis.
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cytosporones coumarins and an alkaloid from the endophytic fungus Pestalotiopsis sp isolated from the chinese mangrove plant rhizophora mucronata
Bioorganic & Medicinal Chemistry, 2009Co-Authors: Jing Xu, Julia Kjer, Jandirk Sendker, Victor Wray, Huashi Guan, Ruangelie Edrada, Werner E G Muller, Mirko Bayer, Jun Wu, Peter ProkschAbstract:Chemical examination of the endophytic fungus Pestalotiopsis sp., isolated from the leaves of the Chinese mangrove Rhizophora mucronata, yielded 11 new compounds including cytosporones J-N (1-3, 5-6), five new coumarins pestalasins A-E (8-12), and a new alkaloid named pestalotiopsoid A (14), along with the known compounds cytosporone C (4), dothiorelone B (7), and 3-hydroxymethyl-6,8-dimethoxycoumarin (13). The structures of the new compounds were unambiguously elucidated on the basis of extensive spectroscopic data analysis. (c) 2009 Elsevier Ltd. All rights reserved.
Wen-bing Yin - One of the best experts on this subject based on the ideXlab platform.
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genetic manipulation of the cop9 signalosome subunit pfcsne leads to the discovery of pestaloficins in Pestalotiopsis fici
Organic Letters, 2017Co-Authors: Yanjing Zheng, Haining Lyu, Ying Huang, Hongwei Liu, Ling Liu, Yongsheng Che, Xingzhong Liu, Huixi Zou, Wen-bing YinAbstract:By deleting the COP9 signalosome subunit PfcsnE from Pestalotiopsis fici, seven compounds that were newly produced by the mutant could be characterized, including five new structures, pestaloficins A–E (1 and 3–6). Pestaloficin A (1) represents a new type of dimeric cyclohexanone derivative linked through an unprecedented pentacyclic spiral ring.
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Genetic Manipulation of the COP9 Signalosome Subunit PfCsnE Leads to the Discovery of Pestaloficins in Pestalotiopsis fici
2017Co-Authors: Yanjing Zheng, Haining Lyu, Ying Huang, Hongwei Liu, Ling Liu, Yongsheng Che, Xingzhong Liu, Huixi Zou, Wen-bing YinAbstract:By deleting the COP9 signalosome subunit PfcsnE from Pestalotiopsis fici, seven compounds that were newly produced by the mutant could be characterized, including five new structures, pestaloficins A–E (1 and 3–6). Pestaloficin A (1) represents a new type of dimeric cyclohexanone derivative linked through an unprecedented pentacyclic spiral ring
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polyketide production of pestaloficiols and macrodiolide ficiolides revealed by manipulations of epigenetic regulators in an endophytic fungus
Organic Letters, 2016Co-Authors: Haichuan Zhou, Hongwei Liu, Xingzhong Liu, Xiuna Wang, Peng Zhang, Weiwei Zhang, Nancy P Keller, Wen-bing YinAbstract:Regarding targeted disruption of epigenetic regulators, histone methyltransferase and deacetylase in a plant endophytic fungus Pestalotiopsis fici have been uncovered as an unexplored chemical repertoire. Manipulation of epigenetic regulators led to the isolation of 15 new polyketides, including pestaloficiols T–W (1–3 and 5), as well as 11 macrodiolide ficiolides A–K (6–16). Ficiolide K (16) was found to contain a very rare 1,6-anhydro-pyranose moiety. Finally, the biosynthetic origin of macrodiolide was characterized by isotope-labeling experiments.