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Shizhong Yang - One of the best experts on this subject based on the ideXlab platform.
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microbiota and their affiliation with physiochemical characteristics of different subsurface Petroleum Reservoirs
International Biodeterioration & Biodegradation, 2017Co-Authors: Serge Maurice Mbadinga, Jin-feng Liu, Lei Zhou, Shizhong YangAbstract:Abstract Microbial communities and their functions in subsurface Petroleum Reservoirs are crucially important for better understanding of biogeochemical processes and life forms in extreme environment, and developing new strategies and technologies for microbial enhanced energy recovery (MEER) and control of reservoir souring and corrosion in oil production systems as well as bioremediation of contaminated sites. Over the last decades, broad phylogenetic and functional diverse microbial communities of different subsurface oil Reservoirs have been described by using the newly available molecular techniques, but the information has been scattered in individual publications. In this review, we present a synthesis on critical analysis of the bacterial and archaeal composition and diversity in oil reservoir systems for a more comprehensive understanding of microbiota and their affiliation to specific oil reservoir conditions, including temperature, salinity and production practices. In particular the frequently reported core and common species of microorganisms with specific characteristics of oil Reservoirs including temperature, water flooding, oil and water phases were identified. This review gives an up-to-date information synthesis of the current data available and also a discussion on the challenging questions and the future research needs.
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dominance of desulfotignum in sulfate reducing community in high sulfate production water of high temperature and corrosive Petroleum Reservoirs
International Biodeterioration & Biodegradation, 2016Co-Authors: Jin-feng Liu, Shizhong Yang, Feng Yao, Serge Maurice MbadingaAbstract:Abstract Petroleum Reservoirs souring and pipelines corrosion are severe in Jiangsu Oilfield of China, and microorganisms have a major role in the related problems. This study revealed the microbial diversity and composition of both bacteria and archaea by using 16S rRNA gene clone library method, and those of sulfate-reducing prokaryotes (SRP) by dissimilatory sulfate reductase α-subunit encoding gene ( dsr ) clone library in two different samples of high temperature Petroleum Reservoirs containing high concentration of sulfate and with known corrosion. In addition, the abundance of bacteria and SRP in the samples was quantified by real-time PCR assays based on 16S rRNA and dsr genes. The results showed that both production water samples from Wei 2-53 and Wei 11-7 wells exhibited very rich bacterial diversity, and both Gammaproteobacteria and Alphaproteobacteria were the main abundant ones. Thioalkalivibrio -related species belonging to Gammaproteobacteria were also observed in both of them. Methanomicrobia , Archaeoglobi and Thermococci were common archaea in these samples, with Archaeoglobus as the dominant genus in both of them. The dsrA libraries analysis showed that a low diversity of sulfidogenic communities with Desulfotignum spp. as the most dominant SRP in the samples. The data reported here enhance our knowledge on microbial community and abundance including SRP in high temperature and corrosive Reservoirs, providing a better basis for further research on and management control of reservoir souring and biocorrosion in oil production systems.
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molecular analysis of the microbial community structures in water flooding Petroleum Reservoirs with different temperatures
Biogeosciences, 2012Co-Authors: Li Ying Wang, Jin-feng Liu, R Y Duan, Shizhong YangAbstract:Abstract. Analyses of microbial communities from six water-flooding Petroleum Reservoirs at temperatures from 21 to 63 °C by 16S rRNA gene clone libraries indicates the presence of physiologically diverse and temperature-dependent microorganisms in these subterrestrial ecosystems. In samples originating from high-temperature Petroleum Reservoirs, most of the archaeal sequences belong to thermophiles affiliated with members of the genera Thermococcus, Methanothermobacter and the order Thermoplasmatales, whereas bacterial sequences predominantly belong to the phyla Firmicutes, Thermotogae and Thermodesulfobacteria. In contrast to high-temperature Petroleum Reservoirs, microorganisms belonging to the Proteobacteria, Methanobacteriales and Methanomicrobiales were the most encountered in samples collected from low-temperature Petroleum Reservoirs. Canonical correspondence analysis (CCA) revealed that temperature, mineralization, ionic type as well as volatile fatty acids showed correlation with the microbial community structures, in particular members of the Firmicutes and the genus Methanothermobacter showed positive correlation with temperature and the concentration of acetate. Overall, these data indicate the large occurrence of hydrogenotrophic methanogens in Petroleum Reservoirs and imply that acetate metabolism via syntrophic oxidation may represent the main methanogenic pathway in high-temperature Petroleum Reservoirs.
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molecular phylogenetic diversity of the microbial community associated with a high temperature Petroleum reservoir at an offshore oilfield
FEMS Microbiology Ecology, 2007Co-Authors: Shizhong Yang, Zhaofeng Rong, Jie ZhangAbstract:The microbial community and its diversity in production water from a high-temperature, water-flooded Petroleum reservoir of an offshore oilfield in China were characterized by 16S rRNA gene sequence analysis. The bacterial and archaeal 16S rRNA gene clone libraries were constructed from the community DNA and, using sequence analysis, 388 bacterial and 220 archaeal randomly selected clones were clustered with 60 and 28 phylotypes, respectively. The results showed that the 16S rRNA genes of bacterial clones belonged to the divisions Firmicutes, Thermotogae, Nitrospirae and Proteobacteria, whereas the archaeal library was dominated by methanogen-like rRNA genes (Methanothermobacter, Methanobacter, Methanobrevibacter and Methanococcus), with a lower percentage of clones belonging to Thermoprotei. Thermophilic microorganisms were found in the production water, as well as mesophilic microorganisms such as Pseudomonas and Acinetobacter-like clones. The thermophilic microorganisms may be common inhabitants of geothermally heated specialized subsurface environments, which have been isolated previously from a number of high-temperature Petroleum Reservoirs worldwide. The mesophilic microorganisms were probably introduced into the reservoir as it was being exploited. The results of this work provide further insight into the composition of microbial communities of high-temperature Petroleum Reservoirs at offshore oilfields.
Jin-feng Liu - One of the best experts on this subject based on the ideXlab platform.
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microbiota and their affiliation with physiochemical characteristics of different subsurface Petroleum Reservoirs
International Biodeterioration & Biodegradation, 2017Co-Authors: Serge Maurice Mbadinga, Jin-feng Liu, Lei Zhou, Shizhong YangAbstract:Abstract Microbial communities and their functions in subsurface Petroleum Reservoirs are crucially important for better understanding of biogeochemical processes and life forms in extreme environment, and developing new strategies and technologies for microbial enhanced energy recovery (MEER) and control of reservoir souring and corrosion in oil production systems as well as bioremediation of contaminated sites. Over the last decades, broad phylogenetic and functional diverse microbial communities of different subsurface oil Reservoirs have been described by using the newly available molecular techniques, but the information has been scattered in individual publications. In this review, we present a synthesis on critical analysis of the bacterial and archaeal composition and diversity in oil reservoir systems for a more comprehensive understanding of microbiota and their affiliation to specific oil reservoir conditions, including temperature, salinity and production practices. In particular the frequently reported core and common species of microorganisms with specific characteristics of oil Reservoirs including temperature, water flooding, oil and water phases were identified. This review gives an up-to-date information synthesis of the current data available and also a discussion on the challenging questions and the future research needs.
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dominance of desulfotignum in sulfate reducing community in high sulfate production water of high temperature and corrosive Petroleum Reservoirs
International Biodeterioration & Biodegradation, 2016Co-Authors: Jin-feng Liu, Shizhong Yang, Feng Yao, Serge Maurice MbadingaAbstract:Abstract Petroleum Reservoirs souring and pipelines corrosion are severe in Jiangsu Oilfield of China, and microorganisms have a major role in the related problems. This study revealed the microbial diversity and composition of both bacteria and archaea by using 16S rRNA gene clone library method, and those of sulfate-reducing prokaryotes (SRP) by dissimilatory sulfate reductase α-subunit encoding gene ( dsr ) clone library in two different samples of high temperature Petroleum Reservoirs containing high concentration of sulfate and with known corrosion. In addition, the abundance of bacteria and SRP in the samples was quantified by real-time PCR assays based on 16S rRNA and dsr genes. The results showed that both production water samples from Wei 2-53 and Wei 11-7 wells exhibited very rich bacterial diversity, and both Gammaproteobacteria and Alphaproteobacteria were the main abundant ones. Thioalkalivibrio -related species belonging to Gammaproteobacteria were also observed in both of them. Methanomicrobia , Archaeoglobi and Thermococci were common archaea in these samples, with Archaeoglobus as the dominant genus in both of them. The dsrA libraries analysis showed that a low diversity of sulfidogenic communities with Desulfotignum spp. as the most dominant SRP in the samples. The data reported here enhance our knowledge on microbial community and abundance including SRP in high temperature and corrosive Reservoirs, providing a better basis for further research on and management control of reservoir souring and biocorrosion in oil production systems.
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Comparison of bacterial community in aqueous and oil phases of water-flooded Petroleum Reservoirs using pyrosequencing and clone library approaches
Applied Microbiology and Biotechnology, 2014Co-Authors: Li Ying Wang, Wen Ji Ke, Xiao Bo Sun, Jin-feng Liu, Ji-dong Gu, Bo-zhong MuAbstract:Bacterial communities in both aqueous and oil phases of water-flooded Petroleum Reservoirs were characterized by molecular analysis of bacterial 16S rRNA genes obtained from Shengli Oil Field using DNA pyrosequencing and gene clone library approaches. Metagenomic DNA was extracted from the aqueous and oil phases and subjected to polymerase chain reaction amplification with primers targeting the bacterial 16S rRNA genes. The analysis by these two methods showed that there was a large difference in bacterial diversity between the aqueous and oil phases of the reservoir fluids, especially in the Reservoirs with lower water cut. At a high phylogenetic level, the predominant bacteria detected by these two approaches were identical. However, pyrosequencing allowed the detection of more rare bacterial species than the clone library method. Statistical analysis showed that the diversity of the bacterial community of the aqueous phase was lower than that of the oil phase. Phylogenetic analysis indicated that the vast majority of sequences detected in the water phase were from members of the genus Arcobacter within the Epsilonproteobacteria, which is capable of degrading the intermediates of hydrocarbon degradation such as acetate. The oil phase of reservoir fluid samples was dominated by members of the genus Pseudomonas within the Gammaproteobacteria and the genus Sphingomonas within the Alphaproteobacteria, which have the ability to degrade crude oil through adherence to hydrocarbons under aerobic conditions. In addition, many anaerobes that could degrade the component of crude oil were also found in the oil phase of reservoir fluids, mainly in the reservoir with lower water cut. These were represented by Desulfovibrio spp., Thermodesulfovibrio spp., Thermodesulforhabdus spp., Thermotoga spp., and Thermoanaerobacterium spp. This research suggested that simultaneous analysis of DNA extracted from both aqueous and oil phases can facilitate a better understanding of the bacterial communities in water-flooded Petroleum Reservoirs.
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diversity and distribution of sulfate reducing bacteria in four Petroleum Reservoirs detected by using 16s rrna and dsrab genes
International Biodeterioration & Biodegradation, 2013Co-Authors: Jing Guan, Li Ying Wang, Liping Xia, Jin-feng LiuAbstract:Abstract Microbial sulfate reduction, an important metabolic process in Petroleum Reservoirs, is widely known as a major contributor to microbial influenced corrosion and deterioration of oil quality. To better control oil field corrosion and oil degradation caused by the sulfate-reducing bacteria (SRBs), the community structure and composition of SRBs in four oil Reservoirs were investigated in this study by comparing clone libraries of 16S rRNA and dissimilatory sulfate reductase ( dsrAB ) genes. In addition, canonical correspondence analysis (CCA) was also employed to find relationship between biodata and physiochemical information. More information on SRB communities was obtained from nested-PCR-phylogenetic analyses of 16S rRNA genes and PCR primer sets amplifying six groups of SRBs frequently detected in oilfields all over the world were used. Amplified sequences belonging to Desulfotomaculum and Desulfobacter were the most dominant in all four Reservoirs. The diversity of SRB communities increased while the temperature of the four oil Reservoirs decreased from 63 to 21. Correlations between environmental variables and species distribution indicated that Desulfotomaculum was correlated with temperature, depth, and the concentration of acetate, propionate and sulphate. Desulfomicrobium , Desulfobacter and Desulfobulbus showed positive correlation with sulphur and salinity. Desulfobacterium was influenced by both salinity and the concentration of acetate. The results of this study provided important information on the microbial ecology of sulfate-reducing bacteria in different Petroleum Reservoirs.
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molecular analysis of the microbial community structures in water flooding Petroleum Reservoirs with different temperatures
Biogeosciences, 2012Co-Authors: Li Ying Wang, Jin-feng Liu, R Y Duan, Shizhong YangAbstract:Abstract. Analyses of microbial communities from six water-flooding Petroleum Reservoirs at temperatures from 21 to 63 °C by 16S rRNA gene clone libraries indicates the presence of physiologically diverse and temperature-dependent microorganisms in these subterrestrial ecosystems. In samples originating from high-temperature Petroleum Reservoirs, most of the archaeal sequences belong to thermophiles affiliated with members of the genera Thermococcus, Methanothermobacter and the order Thermoplasmatales, whereas bacterial sequences predominantly belong to the phyla Firmicutes, Thermotogae and Thermodesulfobacteria. In contrast to high-temperature Petroleum Reservoirs, microorganisms belonging to the Proteobacteria, Methanobacteriales and Methanomicrobiales were the most encountered in samples collected from low-temperature Petroleum Reservoirs. Canonical correspondence analysis (CCA) revealed that temperature, mineralization, ionic type as well as volatile fatty acids showed correlation with the microbial community structures, in particular members of the Firmicutes and the genus Methanothermobacter showed positive correlation with temperature and the concentration of acetate. Overall, these data indicate the large occurrence of hydrogenotrophic methanogens in Petroleum Reservoirs and imply that acetate metabolism via syntrophic oxidation may represent the main methanogenic pathway in high-temperature Petroleum Reservoirs.
Steve Larter - One of the best experts on this subject based on the ideXlab platform.
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The controls on the composition of biodegraded oils in the deep subsurface - Part 3. The impact of microorganism distribution on Petroleum geochemical gradients in biodegraded Petroleum Reservoirs
Organic Geochemistry, 2013Co-Authors: Barry Bennett, J. Adams, Neil D. Gray, Angela Sherry, Thomas B. P. Oldenburg, Haiping Huang, Steve Larter, Ian M. HeadAbstract:Abstract A combined geochemical, geological and microbiological analysis of an actively biodegrading 24.5 m thick oil column in a Canadian heavy oil reservoir has been carried out. The reservoir properties associated with the cored vertical well are characterised by a 15.75 m thick oil column and an 8.75 m zone of steadily decreasing oil saturation below the oil column, referred to as the oil–water transition zone (OWTZ), grading down into a thin water leg. The oil column exhibits systematic gradients in oil physical properties and hydrocarbon composition and shows variations in biodegradation level throughout the reservoir consistent with the notion that the biodegradation of oil is focussed in a bioreactor zone at the base of the oil column. Through the oil column, the dead oil viscosity measured at 20 °C ranged from 50,000 cP (0.05 McP) at the top of the oil column to 1.4 McP at the oil–OWTZ contact, and continued to increase to 10.5 McP within the OWTZ. The saturated and aromatic hydrocarbons are characterised by systematically decreasing bulk fraction and component concentrations down through the oil column. Different compound classes decreased to levels below their detection limit at different depths within the OWTZ, defining a likely bioreactor extent of over 5 m in depth with, for example, n-alkanes being reduced to their detection limit concentration at the bottom of the oil column/top of the OWTZ, while branched isoprenoid alkanes were not completely degraded until well into the OWTZ. Core samples from the oil column and the lower part of the OWTZ were estimated to contain ca. 104–105 bacterial cells/g, based on qPCR of bacterial 16S rRNA genes, while samples from a narrow interval in the OWTZ immediately below the oil column contained on the order of 106–107 cells/g of sediment. Interestingly, these latter numbers are typical of those observed in active deep subsurface biosphere systems with the notion that microbial activity and abundance in the deep subsurface is elevated at geochemical interfaces. The numbers of organisms are not constant throughout the OWTZ. The highest bacterial abundance and geochemical gradients of, for example, methylphenanthrene biodegradation define a zone near the oil–water contact as likely the most active in terms of biodegradation. The largest bacterial abundances in the upper part of the OWTZ are in line with the trend of bacterial abundance with depth that has emerged from extensive analysis of microbial cells in deep subsurface sediments, implying that in terms of deep biosphere cell abundance, oil Reservoirs are similar to other deep subsurface microbial environments. This is puzzling, given the atypical abundance of organic carbon in Petroleum Reservoirs and may imply a common large scale control on microbial abundance and activity in the deep biosphere, including in oilfields.
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Hydrocarbon Degradation in Petroleum Reservoirs.
2010Co-Authors: Ian M. Head, J. Adams, Neil D. Gray, Angela Sherry, Haiping Huang, Steve Larter, David Jones, Arlene K. Rowan, Wilfred F. M. RölingAbstract:In-reservoir Petroleum biodegradation has resulted in vast deposits of heavy oil around the globe. Extraction of heavy oil is more costly and less efficient than conventional oil production and consequently oil biodegradation on geological timescales has significant economic and environmental impacts. The processes that have led to the biodegradation of oil in situ have only recently begun to be elucidated and we have at best a qualitative understanding of the factors that promote oil biodegradation in Petroleum Reservoirs. A synthesis of current thinking on the mechanisms of, and controls on, in-reservoir oil biodegradation is presented. This is placed in the context of oil-field microbiology and used to identify potentially fruitful avenues of research required to fill some of the gaps in our knowledge.
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biodegradation gas destruction and methane generation in deep subsurface Petroleum Reservoirs an overview
Geological Society London Petroleum Geology Conference series, 2005Co-Authors: Barry Bennett, Haiping Huang, Steve Larter, Ian M. Head, Martin Jones, Andrew C Aplin, A MurrayAbstract:Plate tectonics forms and destroys sedimentary basins, accumulating organic carbon and converting it into mobile Petroleum which may be concentrated in reservoir traps in which, if temperatures are below 80°C, it may become biologically degraded (biodegraded). The biodegradation process produces altered, denser, heavy oils and methane as a primary product. Much of the world’s oil is biodegraded under anaerobic conditions, with methane being a major by-product of the action of the deep biosphere on Petroleum when sulphate is not present as an oxidant. A review of the literature relating to destruction of wet gas and the systematics of methane generation during subsurface oil biodegradation concludes that large biodegrading oil fields may be major source systems of dry gas.
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anaerobic hydrocarbon biodegradation in deep subsurface oil Reservoirs
Nature, 2004Co-Authors: Carolyn M Aitken, Steve Larter, D M JonesAbstract:Biodegradation of crude oil in subsurface Petroleum Reservoirs is an important alteration process with major economic consequences. Aerobic degradation of Petroleum hydrocarbons at the surface is well documented and it has long been thought that the flow of oxygen- and nutrient-bearing meteoric waters into Reservoirs was necessary for in-reservoir Petroleum biodegradation. The occurrence of biodegraded oils in Reservoirs where aerobic conditions are unlikely, together with the identification of several anaerobic microorganisms in oil fields and the discovery of anaerobic hydrocarbon biodegradation mechanisms, suggests that anaerobic degradation processes could also be responsible. The extent of anaerobic hydrocarbon degradation processes in the world's deep Petroleum Reservoirs, however, remains strongly contested. Moreover, no organism has yet been isolated that has been shown to degrade hydrocarbons under the conditions found in deep Petroleum Reservoirs. Here we report the isolation of metabolites indicative of anaerobic hydrocarbon degradation from a large fraction of 77 degraded oil samples from both marine and lacustrine sources from around the world, including the volumetrically important Canadian tar sands. Our results therefore suggest that anaerobic hydrocarbon degradation is a common process in biodegraded subsurface oil Reservoirs.
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The microbiology of hydrocarbon degradation in subsurface Petroleum Reservoirs: perspectives and prospects.
Research in microbiology, 2003Co-Authors: Wilfred F. M. Röling, Ian M. Head, Steve LarterAbstract:The majority of the Earth's Petroleum resource is partly biodegraded. This is of considerable practical significance and can limit economic exploitation of Petroleum reserves and lead to problems during Petroleum production. Knowledge of the microorganisms present in Petroleum Reservoirs, their physiological properties and the biochemical potential for hydrocarbon degradation benefits successful Petroleum exploration. Anaerobic conditions prevail in Petroleum Reservoirs and biological hydrocarbon degradation is apparently inhibited at temperatures above 80-90 degrees C. We summarise available knowledge and conjecture on the dominant biological processes active during subsurface Petroleum biodegradation.
Huimei Tian - One of the best experts on this subject based on the ideXlab platform.
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Compositions and Abundances of Sulfate-Reducing and Sulfur-Oxidizing Microorganisms in Water-Flooded Petroleum Reservoirs with Different Temperatures in China
Frontiers in Microbiology, 2017Co-Authors: Huimei Tian, Yanshu Li, Yansen Wang, Jiefang Zhou, Guoqiang Li, Zhaohui Chen, Yan Li, Ting MaAbstract:Sulfate-reducing bacteria (SRB) have been studied extensively in the Petroleum industry due to their role in corrosion, but very little is known about sulfur-oxidizing bacteria (SOB), which drive the oxidization of sulfur-compounds produced by the activity of SRB in Petroleum Reservoirs. Here, we surveyed the community structure, diversity and abundance of SRB and SOB simultaneously based on 16S rRNA, dsrB and soxB gene sequencing, and quantitative PCR analyses, respectively in Petroleum Reservoirs with different physicochemical properties. Similar to SRB, SOB were found widely inhabiting the analyzed Reservoirs with high diversity and different structures. The dominant SRB belonged to the classes Deltaproteobacteria and Clostridia, and included the Desulfotignum, Desulfotomaculum, Desulfovibrio, Desulfobulbus and Desulfomicrobium genera. The most frequently detected potential SOB were Sulfurimonas, Thiobacillus, Thioclava, Thiohalomonas and Dechloromonas, and belonged to Betaproteobacteria, Alphaproteobacteria, and Epsilonproteobacteria. Among them, Desulfovibrio, Desulfomicrobium, Thioclava, and Sulfurimonas were highly abundant in the low-temperature Reservoirs, while Desulfotomaculum, Desulfotignum, Thiobacillus, and Dechloromonas were more often present in high-temperature Reservoirs. The relative abundances of SRB and SOB varied and were present at higher proportions in the relatively high-temperature Reservoirs. Canonical correspondence analysis also revealed that the SRB and SOB communities in Reservoirs displayed high niche specificity and were closely related to reservoir temperature, pH of the formation brine, and sulfate concentration. In conclusion, this study extends our knowledge about the distribution of SRB and SOB communities in Petroleum Reservoirs.
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spatial isolation and environmental factors drive distinct bacterial and archaeal communities in different types of Petroleum Reservoirs in china
Scientific Reports, 2016Co-Authors: Peike Gao, Huimei Tian, Yansen Wang, Jinxia Xie, Bing Zeng, Jiefang ZhouAbstract:To investigate the spatial distribution of microbial communities and their drivers in Petroleum reservoir environments, we performed pyrosequencing of microbial partial 16S rRNA, derived from 20 geographically separated water-flooding Reservoirs, and two Reservoirs that had not been flooded, in China. The results indicated that distinct underground microbial communities inhabited the different Reservoirs. Compared with the bacteria, archaeal alpha-diversity was not strongly correlated with the environmental variables. The variation of the bacterial and archaeal community compositions was affected synthetically, by the mining patterns, spatial isolation, reservoir temperature, salinity and pH of the formation brine. The environmental factors explained 64.22% and 78.26% of the total variance for the bacterial and archaeal communities, respectively. Despite the diverse community compositions, shared populations (48 bacterial and 18 archaeal genera) were found and were dominant in most of the oilfields. Potential indigenous microorganisms, including Carboxydibrachium, Thermosinus, and Neptunomonas, were only detected in a reservoir that had not been flooded with water. This study indicates that: 1) the environmental variation drives distinct microbial communities in different Reservoirs; 2) compared with the archaea, the bacterial communities were highly heterogeneous within and among the Reservoirs; and 3) despite the community variation, some microorganisms are dominant in multiple Petroleum Reservoirs.
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differences in microbial community composition between injection and production water samples of water flooding Petroleum Reservoirs
Biogeosciences, 2015Co-Authors: Huimei Tian, Peike Gao, Yuesi Wang, H W SunAbstract:Abstract. Microbial communities in injected water are expected to have significant influence on those of reservoir strata in long-term water flooding Petroleum Reservoirs. To investigate the similarities and differences in microbial communities in injected water and reservoir strata, high-throughput sequencing of microbial partial 16S rRNA of the water samples collected from the wellhead and downhole of injection wells, and from production wells in a homogeneous sandstone reservoir and a heterogeneous conglomerate reservoir were performed. The results indicate that a small number of microbial populations are shared between the water samples from the injection and production wells in the sandstone reservoir, whereas a large number of microbial populations are shared in the conglomerate reservoir. The bacterial and archaeal communities in the reservoir strata have high concentrations, which are similar to those in the injected water. However, microbial population abundance exhibited large differences between the water samples from the injection and production wells. The number of shared populations reflects the influence of microbial communities in injected water on those in reservoir strata to some extent, and show strong association with the unique variation of reservoir environments.
Li Ying Wang - One of the best experts on this subject based on the ideXlab platform.
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Comparison of bacterial community in aqueous and oil phases of water-flooded Petroleum Reservoirs using pyrosequencing and clone library approaches
Applied Microbiology and Biotechnology, 2014Co-Authors: Li Ying Wang, Wen Ji Ke, Xiao Bo Sun, Jin-feng Liu, Ji-dong Gu, Bo-zhong MuAbstract:Bacterial communities in both aqueous and oil phases of water-flooded Petroleum Reservoirs were characterized by molecular analysis of bacterial 16S rRNA genes obtained from Shengli Oil Field using DNA pyrosequencing and gene clone library approaches. Metagenomic DNA was extracted from the aqueous and oil phases and subjected to polymerase chain reaction amplification with primers targeting the bacterial 16S rRNA genes. The analysis by these two methods showed that there was a large difference in bacterial diversity between the aqueous and oil phases of the reservoir fluids, especially in the Reservoirs with lower water cut. At a high phylogenetic level, the predominant bacteria detected by these two approaches were identical. However, pyrosequencing allowed the detection of more rare bacterial species than the clone library method. Statistical analysis showed that the diversity of the bacterial community of the aqueous phase was lower than that of the oil phase. Phylogenetic analysis indicated that the vast majority of sequences detected in the water phase were from members of the genus Arcobacter within the Epsilonproteobacteria, which is capable of degrading the intermediates of hydrocarbon degradation such as acetate. The oil phase of reservoir fluid samples was dominated by members of the genus Pseudomonas within the Gammaproteobacteria and the genus Sphingomonas within the Alphaproteobacteria, which have the ability to degrade crude oil through adherence to hydrocarbons under aerobic conditions. In addition, many anaerobes that could degrade the component of crude oil were also found in the oil phase of reservoir fluids, mainly in the reservoir with lower water cut. These were represented by Desulfovibrio spp., Thermodesulfovibrio spp., Thermodesulforhabdus spp., Thermotoga spp., and Thermoanaerobacterium spp. This research suggested that simultaneous analysis of DNA extracted from both aqueous and oil phases can facilitate a better understanding of the bacterial communities in water-flooded Petroleum Reservoirs.
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diversity and distribution of sulfate reducing bacteria in four Petroleum Reservoirs detected by using 16s rrna and dsrab genes
International Biodeterioration & Biodegradation, 2013Co-Authors: Jing Guan, Li Ying Wang, Liping Xia, Jin-feng LiuAbstract:Abstract Microbial sulfate reduction, an important metabolic process in Petroleum Reservoirs, is widely known as a major contributor to microbial influenced corrosion and deterioration of oil quality. To better control oil field corrosion and oil degradation caused by the sulfate-reducing bacteria (SRBs), the community structure and composition of SRBs in four oil Reservoirs were investigated in this study by comparing clone libraries of 16S rRNA and dissimilatory sulfate reductase ( dsrAB ) genes. In addition, canonical correspondence analysis (CCA) was also employed to find relationship between biodata and physiochemical information. More information on SRB communities was obtained from nested-PCR-phylogenetic analyses of 16S rRNA genes and PCR primer sets amplifying six groups of SRBs frequently detected in oilfields all over the world were used. Amplified sequences belonging to Desulfotomaculum and Desulfobacter were the most dominant in all four Reservoirs. The diversity of SRB communities increased while the temperature of the four oil Reservoirs decreased from 63 to 21. Correlations between environmental variables and species distribution indicated that Desulfotomaculum was correlated with temperature, depth, and the concentration of acetate, propionate and sulphate. Desulfomicrobium , Desulfobacter and Desulfobulbus showed positive correlation with sulphur and salinity. Desulfobacterium was influenced by both salinity and the concentration of acetate. The results of this study provided important information on the microbial ecology of sulfate-reducing bacteria in different Petroleum Reservoirs.
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molecular analysis of the microbial community structures in water flooding Petroleum Reservoirs with different temperatures
Biogeosciences, 2012Co-Authors: Li Ying Wang, Jin-feng Liu, R Y Duan, Shizhong YangAbstract:Abstract. Analyses of microbial communities from six water-flooding Petroleum Reservoirs at temperatures from 21 to 63 °C by 16S rRNA gene clone libraries indicates the presence of physiologically diverse and temperature-dependent microorganisms in these subterrestrial ecosystems. In samples originating from high-temperature Petroleum Reservoirs, most of the archaeal sequences belong to thermophiles affiliated with members of the genera Thermococcus, Methanothermobacter and the order Thermoplasmatales, whereas bacterial sequences predominantly belong to the phyla Firmicutes, Thermotogae and Thermodesulfobacteria. In contrast to high-temperature Petroleum Reservoirs, microorganisms belonging to the Proteobacteria, Methanobacteriales and Methanomicrobiales were the most encountered in samples collected from low-temperature Petroleum Reservoirs. Canonical correspondence analysis (CCA) revealed that temperature, mineralization, ionic type as well as volatile fatty acids showed correlation with the microbial community structures, in particular members of the Firmicutes and the genus Methanothermobacter showed positive correlation with temperature and the concentration of acetate. Overall, these data indicate the large occurrence of hydrogenotrophic methanogens in Petroleum Reservoirs and imply that acetate metabolism via syntrophic oxidation may represent the main methanogenic pathway in high-temperature Petroleum Reservoirs.