The Experts below are selected from a list of 306 Experts worldwide ranked by ideXlab platform
M. Zeidan - One of the best experts on this subject based on the ideXlab platform.
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Improved detection of Petunia vein clearing caulimovirus
HortScience, 2000Co-Authors: M. Zeidan, Noga Sikron, J. Cohen, A. GeraAbstract:Petunia vein clearing virus (PVCV), a possible member of the caulimovirus group, was detected in several cultivars of vegetatively propagated Petunias ( Petunia × hybrida Hort. Volm.-Andr.) grown in commercial nurseries. Leaf dip preparations and ultrathin sections of leaf tissue were analyzed by transmission electron microscopy (TEM). Spherical virus particles, 45-50 nm in diameter, were observed in samples taken from symptomatic Petunia plants. The virus was purified and a polyclonal antiserum was prepared. In immuno-specific electron microscopy (ISEM), the PVCV antiserum-treated samples reacted with a distinct decoration on the virus suspect particles. A polymerase chain reaction (PCR)-based assay was used to detect PVCV in total nucleic acid extracts derived from infected Petunia plants. Two primer pairs were designed to flank a 736-base- pair sequence located in the RNA-dependent RNA polymerase gene of the PVCV genome. A DNA fragment of predicted size was visualized in agarose gels. The authenticity of the amplified DNA fragment was confirmed by restriction analysis and by hybridization with the virus-specific PVCV DNA probe. The virus could be detected efficiently in high dilutions of sap extracted from infected Petunia plants .
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First Report of Petunia vein clearing virus in Israel.
Plant disease, 2000Co-Authors: A. Gera, J. Cohen, Noga Sikron, M. ZeidanAbstract:Petunia vein clearing virus (PVCV), a possible member of the cauli-movirus group, was detected in several cultivars of vegetatively propagated Petunias grown in commercial nurseries in Israel. Symptoms associated with PVCV infection in Petunia cv. Chaplin consisted of stunted young shoots, leaf yellowing, and severe vein clearing. Virus incidence within this cultivar was 40 to 60%. Symptoms were most frequently expressed when plants were subjected to temperatures above 20°C and nutrient deficiencies. The virus was transmitted to Petunia only by grafting, however, not by aphids or mechanical inoculation. Leaf dip preparations and thin sections of leaf tissue were analyzed by transmission electron microscopy. Spherical virus particles (45 to 50 nm diameter) were observed in samples from symptomatic Petunia plants. In immunoelectron microscopy, the virus particles produced a distinct pattern with the specific antiserum against PVCV provided by D. Lesemann (Biologische Bundesanstalt, Braunschweig, Germany). I...
D. E. Lesemann - One of the best experts on this subject based on the ideXlab platform.
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Occurrence of Petunia Vein-Clearing Virus in the U.S.A.
Plant disease, 1998Co-Authors: B. E. L. Lockhart, D. E. LesemannAbstract:Petunia vein-clearing virus (PVCV), a tentative member of the caulimovirus group of plant pararetroviruses, was first identified in Petunia (Petunia hybrida Vilm.) in Germany in the hybrid cv. Himmelroschen (1). A similar virus was recently identified in Minnesota in the Petunia cv. Fantasy Pink grown from seed in commercial greenhouses. This virus has spherical particles 46 to 47 nm in diameter in preparations negatively stained with 1% uranyl acetate or sodium phosphotungstate, pH 7.0, and which contain a double-stranded DNA genome approximately 7.3 kb in size. The virus was shown by immunoelectron microscopy (IEM) to be closely related serologically to PVCV. No serological relationship to any other caulimoviruses was detected. Like PVCV, which is transmitted only by seed and grafting, the Minnesota virus isolate was not transmitted by mechanical inoculation to Petunia or any other indicator plant. Symptoms associated with infection by PVCV in cv. Fantasy Pink consisted of mild vein clearing to severe v...
A. Gera - One of the best experts on this subject based on the ideXlab platform.
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Improved detection of Petunia vein clearing caulimovirus
HortScience, 2000Co-Authors: M. Zeidan, Noga Sikron, J. Cohen, A. GeraAbstract:Petunia vein clearing virus (PVCV), a possible member of the caulimovirus group, was detected in several cultivars of vegetatively propagated Petunias ( Petunia × hybrida Hort. Volm.-Andr.) grown in commercial nurseries. Leaf dip preparations and ultrathin sections of leaf tissue were analyzed by transmission electron microscopy (TEM). Spherical virus particles, 45-50 nm in diameter, were observed in samples taken from symptomatic Petunia plants. The virus was purified and a polyclonal antiserum was prepared. In immuno-specific electron microscopy (ISEM), the PVCV antiserum-treated samples reacted with a distinct decoration on the virus suspect particles. A polymerase chain reaction (PCR)-based assay was used to detect PVCV in total nucleic acid extracts derived from infected Petunia plants. Two primer pairs were designed to flank a 736-base- pair sequence located in the RNA-dependent RNA polymerase gene of the PVCV genome. A DNA fragment of predicted size was visualized in agarose gels. The authenticity of the amplified DNA fragment was confirmed by restriction analysis and by hybridization with the virus-specific PVCV DNA probe. The virus could be detected efficiently in high dilutions of sap extracted from infected Petunia plants .
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First Report of Petunia vein clearing virus in Israel.
Plant disease, 2000Co-Authors: A. Gera, J. Cohen, Noga Sikron, M. ZeidanAbstract:Petunia vein clearing virus (PVCV), a possible member of the cauli-movirus group, was detected in several cultivars of vegetatively propagated Petunias grown in commercial nurseries in Israel. Symptoms associated with PVCV infection in Petunia cv. Chaplin consisted of stunted young shoots, leaf yellowing, and severe vein clearing. Virus incidence within this cultivar was 40 to 60%. Symptoms were most frequently expressed when plants were subjected to temperatures above 20°C and nutrient deficiencies. The virus was transmitted to Petunia only by grafting, however, not by aphids or mechanical inoculation. Leaf dip preparations and thin sections of leaf tissue were analyzed by transmission electron microscopy. Spherical virus particles (45 to 50 nm diameter) were observed in samples from symptomatic Petunia plants. In immunoelectron microscopy, the virus particles produced a distinct pattern with the specific antiserum against PVCV provided by D. Lesemann (Biologische Bundesanstalt, Braunschweig, Germany). I...
B. E. L. Lockhart - One of the best experts on this subject based on the ideXlab platform.
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Occurrence of Petunia Vein-Clearing Virus in the U.S.A.
Plant disease, 1998Co-Authors: B. E. L. Lockhart, D. E. LesemannAbstract:Petunia vein-clearing virus (PVCV), a tentative member of the caulimovirus group of plant pararetroviruses, was first identified in Petunia (Petunia hybrida Vilm.) in Germany in the hybrid cv. Himmelroschen (1). A similar virus was recently identified in Minnesota in the Petunia cv. Fantasy Pink grown from seed in commercial greenhouses. This virus has spherical particles 46 to 47 nm in diameter in preparations negatively stained with 1% uranyl acetate or sodium phosphotungstate, pH 7.0, and which contain a double-stranded DNA genome approximately 7.3 kb in size. The virus was shown by immunoelectron microscopy (IEM) to be closely related serologically to PVCV. No serological relationship to any other caulimoviruses was detected. Like PVCV, which is transmitted only by seed and grafting, the Minnesota virus isolate was not transmitted by mechanical inoculation to Petunia or any other indicator plant. Symptoms associated with infection by PVCV in cv. Fantasy Pink consisted of mild vein clearing to severe v...
Scott Adkins - One of the best experts on this subject based on the ideXlab platform.
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surprising results from a search for effective disinfectants for tobacco mosaic virus contaminated tools
Plant Disease, 2010Co-Authors: Dennis J Lewandowski, Amanda J Hayes, Scott AdkinsAbstract:AbstractOver 100 years after its discovery, Tobacco mosaic virus (TMV) remains an economically important pathogen for producers of many vegetatively propagated crops including Petunias (Petunia × hybrida). To directly address this concern, we have developed a robust system to determine efficacy of disinfectants for treating TMV-contaminated cutting tools using a combination of preliminary screens and replicated trials. Contrary to widely held beliefs, wild-type (wt) TMV and four additional tobamovirus species infected four Petunia cultivars without producing obvious viral symptoms. In contrast, a Petunia isolate of TMV with 99.0% (nucleotide) and 99.4% (amino acid) coat protein sequence identity to wt TMV produced symptoms on all but one tested cultivar. We also show that TMV transmission can occur up to the twentieth Petunia plant cut following a single cutting event on a TMV-infected plant. Although many new products are now available, treatment of TMV-contaminated tools with a 20% (wt/vol) solution of ...