The Experts below are selected from a list of 88989 Experts worldwide ranked by ideXlab platform

Anan Srikiatkhachorn - One of the best experts on this subject based on the ideXlab platform.

Ukkrit Jansri - One of the best experts on this subject based on the ideXlab platform.

Yoshihiro Ito - One of the best experts on this subject based on the ideXlab platform.

  • In vitro adaptation of a ligase ribozyme for activity under a low‐Ph Condition
    Biotechnology and Bioengineering, 2001
    Co-Authors: Yoshitaka Miyamoto, Naozumi Teramoto, Yukio Imanishi, Yoshihiro Ito
    Abstract:

    A ligase ribozyme accelerating a ligation reaction with oligonucleotide under a low-Ph Condition was selected by in vitro adaptation. A ribozyme active at Ph 7 was randomly mutated, and the resultant RNA library was subjected to in vitro adaptation under a low-Ph reaction Condition. At Ph 4, the adapted RNAs reacted with the oligonucleotide substrates about 200 times faster than the original ribozyme. When the ribozyme was cloned and sequenced, 10 of the 30 clones sequenced had identical sequences. The differences in sequence from the original ribozyme were found at four positions in the middle region and at the 3' end. A few sequential differences dominated the activity of the ribozyme under the extreme Condition. The adapted ribozyme had one repeating sequence that was critical for the activity.

  • in vitro adaptation of a ligase ribozyme for activity under a low Ph Condition
    Biotechnology and Bioengineering, 2001
    Co-Authors: Yoshitaka Miyamoto, Naozumi Teramoto, Yukio Imanishi, Yoshihiro Ito
    Abstract:

    A ligase ribozyme accelerating a ligation reaction with oligonucleotide under a low-Ph Condition was selected by in vitro adaptation. A ribozyme active at Ph 7 was randomly mutated, and the resultant RNA library was subjected to in vitro adaptation under a low-Ph reaction Condition. At Ph 4, the adapted RNAs reacted with the oligonucleotide substrates about 200 times faster than the original ribozyme. When the ribozyme was cloned and sequenced, 10 of the 30 clones sequenced had identical sequences. The differences in sequence from the original ribozyme were found at four positions in the middle region and at the 3' end. A few sequential differences dominated the activity of the ribozyme under the extreme Condition. The adapted ribozyme had one repeating sequence that was critical for the activity.

Takashi Hayashita - One of the best experts on this subject based on the ideXlab platform.

  • Selective Sugar Recognition by Anthracene-Type Boronic Acid FluoroPhore/Cyclodextrin Supramolecular Complex Under Physiological Ph Condition.
    Frontiers in Chemistry, 2019
    Co-Authors: Ko Sugita, Yuji Tsuchido, Chisato Kasahara, Maria Antonietta Casulli, Shoji Fujiwara, Takeshi Hashimoto, Takashi Hayashita
    Abstract:

    We synthesized novel PET (Photoinduced electron transfer) type fluorescence glucose probe 1 ((4-(anthracen-2-yl-carbamoyl)-3-fluoroPhenyl)boronic acid), which has a Phenylboronic acid (PBA) moiety as the recognition site and anthracene as the fluorescent part. Although the PBA derivatives dissociate and bind with sugar in the basic Condition, our new fluorescent probe can recognize sugars in the Physiological Ph by introducing an electron-withdrawing fluorine group into the PBA moiety. As a result, the pKa value of this fluorescent probe was lowered and the probe was able to recognize sugars at the Physiological Ph of 7.4. The sensor was found to produce two types of fluorescent signals, monomer fluorescence and dimer fluorescence, by forming a supramolecular 2:1 complex of 1 with glucose inside a gamma-cyclodextrin (gamma-CyD) cavity. Selective ratiometric sensing of glucose by the 1/gamma-CyD complex was achieved in water at Physiological Ph.

  • selective sugar recognition by anthracene type boronic acid fluoroPhore cyclodextrin supramolecular complex under Physiological Ph Condition
    Frontiers in Chemistry, 2019
    Co-Authors: Ko Sugita, Yuji Tsuchido, Chisato Kasahara, Maria Antonietta Casulli, Shoji Fujiwara, Takeshi Hashimoto, Takashi Hayashita
    Abstract:

    We synthesized novel PET (Photoinduced electron transfer) type fluorescence glucose probe 1 ((4-(anthracen-2-yl-carbamoyl)-3-fluoroPhenyl)boronic acid), which has a Phenylboronic acid (PBA) moiety as the recognition site and anthracene as the fluorescent part. Although the PBA derivatives dissociate and bind with sugar in the basic Condition, our new fluorescent probe can recognize sugars in the Physiological Ph by introducing an electron-withdrawing fluorine group into the PBA moiety. As a result, the pKa value of this fluorescent probe was lowered and the probe was able to recognize sugars at the Physiological Ph of 7.4. The sensor was found to produce two types of fluorescent signals, monomer fluorescence and dimer fluorescence, by forming a supramolecular 2:1 complex of 1 with glucose inside a gamma-cyclodextrin (gamma-CyD) cavity. Selective ratiometric sensing of glucose by the 1/gamma-CyD complex was achieved in water at Physiological Ph.

Yuji Tsuchido - One of the best experts on this subject based on the ideXlab platform.

  • Selective Sugar Recognition by Anthracene-Type Boronic Acid FluoroPhore/Cyclodextrin Supramolecular Complex Under Physiological Ph Condition.
    Frontiers in Chemistry, 2019
    Co-Authors: Ko Sugita, Yuji Tsuchido, Chisato Kasahara, Maria Antonietta Casulli, Shoji Fujiwara, Takeshi Hashimoto, Takashi Hayashita
    Abstract:

    We synthesized novel PET (Photoinduced electron transfer) type fluorescence glucose probe 1 ((4-(anthracen-2-yl-carbamoyl)-3-fluoroPhenyl)boronic acid), which has a Phenylboronic acid (PBA) moiety as the recognition site and anthracene as the fluorescent part. Although the PBA derivatives dissociate and bind with sugar in the basic Condition, our new fluorescent probe can recognize sugars in the Physiological Ph by introducing an electron-withdrawing fluorine group into the PBA moiety. As a result, the pKa value of this fluorescent probe was lowered and the probe was able to recognize sugars at the Physiological Ph of 7.4. The sensor was found to produce two types of fluorescent signals, monomer fluorescence and dimer fluorescence, by forming a supramolecular 2:1 complex of 1 with glucose inside a gamma-cyclodextrin (gamma-CyD) cavity. Selective ratiometric sensing of glucose by the 1/gamma-CyD complex was achieved in water at Physiological Ph.

  • selective sugar recognition by anthracene type boronic acid fluoroPhore cyclodextrin supramolecular complex under Physiological Ph Condition
    Frontiers in Chemistry, 2019
    Co-Authors: Ko Sugita, Yuji Tsuchido, Chisato Kasahara, Maria Antonietta Casulli, Shoji Fujiwara, Takeshi Hashimoto, Takashi Hayashita
    Abstract:

    We synthesized novel PET (Photoinduced electron transfer) type fluorescence glucose probe 1 ((4-(anthracen-2-yl-carbamoyl)-3-fluoroPhenyl)boronic acid), which has a Phenylboronic acid (PBA) moiety as the recognition site and anthracene as the fluorescent part. Although the PBA derivatives dissociate and bind with sugar in the basic Condition, our new fluorescent probe can recognize sugars in the Physiological Ph by introducing an electron-withdrawing fluorine group into the PBA moiety. As a result, the pKa value of this fluorescent probe was lowered and the probe was able to recognize sugars at the Physiological Ph of 7.4. The sensor was found to produce two types of fluorescent signals, monomer fluorescence and dimer fluorescence, by forming a supramolecular 2:1 complex of 1 with glucose inside a gamma-cyclodextrin (gamma-CyD) cavity. Selective ratiometric sensing of glucose by the 1/gamma-CyD complex was achieved in water at Physiological Ph.