The Experts below are selected from a list of 261 Experts worldwide ranked by ideXlab platform
Maurice Bartlett Hallett - One of the best experts on this subject based on the ideXlab platform.
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localised ptdins 3 4 5 p3 or ptdins 3 4 p2 at the Phagocytic cup is required for both phagosome closure and ca2 signalling in hl60 neutrophils
Journal of Cell Science, 2006Co-Authors: Sharon Dewitt, Wei Tian, Maurice Bartlett HallettAbstract:Several events accompany integrin-mediated phagocytosis by myeloid cells. These include local pseudopod and Phagocytic cup formation followed by Ca2+ signalling. However, there is also a role for localised phosphatidylinositol (3,4,5) trisphosphate [PtdIns(3,4,5) P 3] production. Here we report that in neutrophilic HL-60 cells expressing PH-Akt-GFP, binding of iC3b-coated zymosan particles (2 μm in diameter) via β2 integrin induces an incomplete Phagocytic cup to form before either PtdIns(3,4,5) P 3 or phosphatidylinositol (3,4) bisphosphate [PtdIns(3,4) P 2] production or Ca2+ signalling. These phosphoinositides then accumulated locally at the site of the Phagocytic cup and Ca2+ signalling and phagosome closure follows immediately. Although photobleaching showed that PH-Akt-GFP was freely diffusible in the cytosol and able to dissociate from the Phagocytic cup, it was restricted to the plasma membrane of the formed but open phagosome and failed to diffuse into the surrounding plasma membrane or neighbouring Phagocytic cups even if connected. Inhibition of phosphoinositide (PI) 3-kinase or depletion of membrane cholesterol inhibited both Ca2+ signalling and phagosome closure, but had no effect on particle binding or Phagocytic cup formation. We therefore conclude that PtdIns(3,4,5) P 3 or PtdIns(3,4) P 2 generation was not required for the events that initiate the formation of the Phagocytic cup, but that anchoring of PtdIns(3,4,5) P 3 at the Phagocytic cup is an essential step for phagosome closure and Ca2+ signalling.
Eduardo Ortega - One of the best experts on this subject based on the ideXlab platform.
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Seasonal variations in the immune system of the cyprinid Tinca tinca. Phagocytic function
Comparative immunology microbiology and infectious diseases, 1995Co-Authors: M.e. Collazos, Carmen Barriga, Eduardo OrtegaAbstract:Seasonal variations in the in vitro Phagocytic process of blood granulocytes from the tench Tinca tinca were examined. Different stages of the Phagocytic process: mobility rate, attachment, ingestion and killing of Candida albicans were evaluated. Tench were kept in natural ponds in ambient water temperature, and the in vitro assays were performed at both 22 degrees C and the relative ambient temperature. Results between the seasonal samples were then compared. In vitro induced mobility, attachment, ingestion and killing of C. albicans showed strong seasonal variations, furthermore, the Phagocytic process at 22 degrees C varied significantly according to season. Phagocytic activity from samples taken during the spring demonstrated the highest activity at 22 degrees C, whilst greatest activity at seasonal temperature, in terms of mobility rate, Phagocytic index and microbicide capacity, occurred during the winter. These results are consistent with the hypothesis that phagocytosis in fish is resistant to low temperatures.
Sharon Dewitt - One of the best experts on this subject based on the ideXlab platform.
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localised ptdins 3 4 5 p3 or ptdins 3 4 p2 at the Phagocytic cup is required for both phagosome closure and ca2 signalling in hl60 neutrophils
Journal of Cell Science, 2006Co-Authors: Sharon Dewitt, Wei Tian, Maurice Bartlett HallettAbstract:Several events accompany integrin-mediated phagocytosis by myeloid cells. These include local pseudopod and Phagocytic cup formation followed by Ca2+ signalling. However, there is also a role for localised phosphatidylinositol (3,4,5) trisphosphate [PtdIns(3,4,5) P 3] production. Here we report that in neutrophilic HL-60 cells expressing PH-Akt-GFP, binding of iC3b-coated zymosan particles (2 μm in diameter) via β2 integrin induces an incomplete Phagocytic cup to form before either PtdIns(3,4,5) P 3 or phosphatidylinositol (3,4) bisphosphate [PtdIns(3,4) P 2] production or Ca2+ signalling. These phosphoinositides then accumulated locally at the site of the Phagocytic cup and Ca2+ signalling and phagosome closure follows immediately. Although photobleaching showed that PH-Akt-GFP was freely diffusible in the cytosol and able to dissociate from the Phagocytic cup, it was restricted to the plasma membrane of the formed but open phagosome and failed to diffuse into the surrounding plasma membrane or neighbouring Phagocytic cups even if connected. Inhibition of phosphoinositide (PI) 3-kinase or depletion of membrane cholesterol inhibited both Ca2+ signalling and phagosome closure, but had no effect on particle binding or Phagocytic cup formation. We therefore conclude that PtdIns(3,4,5) P 3 or PtdIns(3,4) P 2 generation was not required for the events that initiate the formation of the Phagocytic cup, but that anchoring of PtdIns(3,4,5) P 3 at the Phagocytic cup is an essential step for phagosome closure and Ca2+ signalling.
M.e. Collazos - One of the best experts on this subject based on the ideXlab platform.
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Seasonal variations in the immune system of the cyprinid Tinca tinca. Phagocytic function
Comparative immunology microbiology and infectious diseases, 1995Co-Authors: M.e. Collazos, Carmen Barriga, Eduardo OrtegaAbstract:Seasonal variations in the in vitro Phagocytic process of blood granulocytes from the tench Tinca tinca were examined. Different stages of the Phagocytic process: mobility rate, attachment, ingestion and killing of Candida albicans were evaluated. Tench were kept in natural ponds in ambient water temperature, and the in vitro assays were performed at both 22 degrees C and the relative ambient temperature. Results between the seasonal samples were then compared. In vitro induced mobility, attachment, ingestion and killing of C. albicans showed strong seasonal variations, furthermore, the Phagocytic process at 22 degrees C varied significantly according to season. Phagocytic activity from samples taken during the spring demonstrated the highest activity at 22 degrees C, whilst greatest activity at seasonal temperature, in terms of mobility rate, Phagocytic index and microbicide capacity, occurred during the winter. These results are consistent with the hypothesis that phagocytosis in fish is resistant to low temperatures.
Wei Tian - One of the best experts on this subject based on the ideXlab platform.
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localised ptdins 3 4 5 p3 or ptdins 3 4 p2 at the Phagocytic cup is required for both phagosome closure and ca2 signalling in hl60 neutrophils
Journal of Cell Science, 2006Co-Authors: Sharon Dewitt, Wei Tian, Maurice Bartlett HallettAbstract:Several events accompany integrin-mediated phagocytosis by myeloid cells. These include local pseudopod and Phagocytic cup formation followed by Ca2+ signalling. However, there is also a role for localised phosphatidylinositol (3,4,5) trisphosphate [PtdIns(3,4,5) P 3] production. Here we report that in neutrophilic HL-60 cells expressing PH-Akt-GFP, binding of iC3b-coated zymosan particles (2 μm in diameter) via β2 integrin induces an incomplete Phagocytic cup to form before either PtdIns(3,4,5) P 3 or phosphatidylinositol (3,4) bisphosphate [PtdIns(3,4) P 2] production or Ca2+ signalling. These phosphoinositides then accumulated locally at the site of the Phagocytic cup and Ca2+ signalling and phagosome closure follows immediately. Although photobleaching showed that PH-Akt-GFP was freely diffusible in the cytosol and able to dissociate from the Phagocytic cup, it was restricted to the plasma membrane of the formed but open phagosome and failed to diffuse into the surrounding plasma membrane or neighbouring Phagocytic cups even if connected. Inhibition of phosphoinositide (PI) 3-kinase or depletion of membrane cholesterol inhibited both Ca2+ signalling and phagosome closure, but had no effect on particle binding or Phagocytic cup formation. We therefore conclude that PtdIns(3,4,5) P 3 or PtdIns(3,4) P 2 generation was not required for the events that initiate the formation of the Phagocytic cup, but that anchoring of PtdIns(3,4,5) P 3 at the Phagocytic cup is an essential step for phagosome closure and Ca2+ signalling.
