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Yuansong Wei - One of the best experts on this subject based on the ideXlab platform.

  • factors influencing the fate of antibiotic resistance genes during thermochemical pretreatment and anaerobic digestion of Pharmaceutical Waste sludge
    Environmental Pollution, 2018
    Co-Authors: Juan Tong, J Zhang, Irini Angelidaki, Yuansong Wei
    Abstract:

    Abstract The prevalence of antibiotic resistance genes (ARGs) in Waste sludge, especially for the Pharmaceutical Waste sludge, presents great potential risks to human health. Although ARGs and factors affecting their spreading are of major importance for human health, the factors influencing the fate of ARGs during sludge treatment, especially for Pharmaceutical sludge treatment are not yet well understood. In order to be able to minimize ARGs spreading, it is important to find what is influencing their spreading. Therefore, certain factors, such as the sludge characteristics, bacterial diversity and community composition, and mobile genetic elements (MGEs) during the advanced AD of Pharmaceutical sludge with different pretreatments were studied, and their affinity with ARGs was elucidated by Spearman correlation analysis. Furthermore, multiple linear regression was introduced to evaluate the importance of the various factors. Results showed that 59.7%–88.3% of the variations in individual ARGs and total ARGs can be explained by the corresponding factors. Bacterial diversity rather than specific bacterial community composition affected the fate of ARGs, whereas alkalinity was the most important factor on ARGs among all sludge characteristics investigated in this study. Besides, 66.4% of variation of total ARGs was driven by the changes of MGEs. Multiple linear regression models also reveal the collective effect of these factors on ARGs, and the contributions of each factor impact on ARGs. This study provides more comprehension about the factors impact on the fate of ARGs during Pharmaceutical sludge treatment, and offers an approach to evaluate the importance of each factor, which method could be introduced for evaluation of factors influencing ARGs during other types of sludge or Wastewater treatment.

  • occurrence of antibiotic resistance genes and mobile genetic elements in enterococci and genomic dna during anaerobic digestion of Pharmaceutical Waste sludge with different pretreatments
    Bioresource Technology, 2017
    Co-Authors: Juan Tong, J Zhang, Qianwen Sui, Rui Wang, Meixue Chen, Yuansong Wei
    Abstract:

    Pharmaceutical Waste sludge harbors large amounts of antibiotic resistance genes (ARGs) and mobile genetic elements (MGEs), and it is necessary to study the reduction of ARGs and MGEs during sludge treatment. Therefore, the antibiotic resistance phenotypes and genotypes of enterococci, and the ARGs and MGEs in genomic DNA were investigated during anaerobic digestion (AD) with microwave (MW), thermal hydrolysis (TH) and ozone pretreatment. Results showed that sludge pretreatment increased the occurrence of the resistance phenotypes and genotypes of enterococci. During AD, the resistance of enterococci to macrolides decreased, except for in the MW-pretreated sludge. Horizontal gene transfer and co-occurrence of ermB and tetM in enterococci resulted in increased tetracycline resistance of enterococci throughout the sludge treatment. MGEs such as intI1, ISCR1 and Tn916/1545 had a significant effect on the distribution of ARGs. AD with pretreatment, especially TH pretreatment, resulted in greater ARGs and MGEs reduction and improved methane production.

Juan Tong - One of the best experts on this subject based on the ideXlab platform.

  • factors influencing the fate of antibiotic resistance genes during thermochemical pretreatment and anaerobic digestion of Pharmaceutical Waste sludge
    Environmental Pollution, 2018
    Co-Authors: Juan Tong, J Zhang, Irini Angelidaki, Yuansong Wei
    Abstract:

    Abstract The prevalence of antibiotic resistance genes (ARGs) in Waste sludge, especially for the Pharmaceutical Waste sludge, presents great potential risks to human health. Although ARGs and factors affecting their spreading are of major importance for human health, the factors influencing the fate of ARGs during sludge treatment, especially for Pharmaceutical sludge treatment are not yet well understood. In order to be able to minimize ARGs spreading, it is important to find what is influencing their spreading. Therefore, certain factors, such as the sludge characteristics, bacterial diversity and community composition, and mobile genetic elements (MGEs) during the advanced AD of Pharmaceutical sludge with different pretreatments were studied, and their affinity with ARGs was elucidated by Spearman correlation analysis. Furthermore, multiple linear regression was introduced to evaluate the importance of the various factors. Results showed that 59.7%–88.3% of the variations in individual ARGs and total ARGs can be explained by the corresponding factors. Bacterial diversity rather than specific bacterial community composition affected the fate of ARGs, whereas alkalinity was the most important factor on ARGs among all sludge characteristics investigated in this study. Besides, 66.4% of variation of total ARGs was driven by the changes of MGEs. Multiple linear regression models also reveal the collective effect of these factors on ARGs, and the contributions of each factor impact on ARGs. This study provides more comprehension about the factors impact on the fate of ARGs during Pharmaceutical sludge treatment, and offers an approach to evaluate the importance of each factor, which method could be introduced for evaluation of factors influencing ARGs during other types of sludge or Wastewater treatment.

  • occurrence of antibiotic resistance genes and mobile genetic elements in enterococci and genomic dna during anaerobic digestion of Pharmaceutical Waste sludge with different pretreatments
    Bioresource Technology, 2017
    Co-Authors: Juan Tong, J Zhang, Qianwen Sui, Rui Wang, Meixue Chen, Yuansong Wei
    Abstract:

    Pharmaceutical Waste sludge harbors large amounts of antibiotic resistance genes (ARGs) and mobile genetic elements (MGEs), and it is necessary to study the reduction of ARGs and MGEs during sludge treatment. Therefore, the antibiotic resistance phenotypes and genotypes of enterococci, and the ARGs and MGEs in genomic DNA were investigated during anaerobic digestion (AD) with microwave (MW), thermal hydrolysis (TH) and ozone pretreatment. Results showed that sludge pretreatment increased the occurrence of the resistance phenotypes and genotypes of enterococci. During AD, the resistance of enterococci to macrolides decreased, except for in the MW-pretreated sludge. Horizontal gene transfer and co-occurrence of ermB and tetM in enterococci resulted in increased tetracycline resistance of enterococci throughout the sludge treatment. MGEs such as intI1, ISCR1 and Tn916/1545 had a significant effect on the distribution of ARGs. AD with pretreatment, especially TH pretreatment, resulted in greater ARGs and MGEs reduction and improved methane production.

J Zhang - One of the best experts on this subject based on the ideXlab platform.

  • factors influencing the fate of antibiotic resistance genes during thermochemical pretreatment and anaerobic digestion of Pharmaceutical Waste sludge
    Environmental Pollution, 2018
    Co-Authors: Juan Tong, J Zhang, Irini Angelidaki, Yuansong Wei
    Abstract:

    Abstract The prevalence of antibiotic resistance genes (ARGs) in Waste sludge, especially for the Pharmaceutical Waste sludge, presents great potential risks to human health. Although ARGs and factors affecting their spreading are of major importance for human health, the factors influencing the fate of ARGs during sludge treatment, especially for Pharmaceutical sludge treatment are not yet well understood. In order to be able to minimize ARGs spreading, it is important to find what is influencing their spreading. Therefore, certain factors, such as the sludge characteristics, bacterial diversity and community composition, and mobile genetic elements (MGEs) during the advanced AD of Pharmaceutical sludge with different pretreatments were studied, and their affinity with ARGs was elucidated by Spearman correlation analysis. Furthermore, multiple linear regression was introduced to evaluate the importance of the various factors. Results showed that 59.7%–88.3% of the variations in individual ARGs and total ARGs can be explained by the corresponding factors. Bacterial diversity rather than specific bacterial community composition affected the fate of ARGs, whereas alkalinity was the most important factor on ARGs among all sludge characteristics investigated in this study. Besides, 66.4% of variation of total ARGs was driven by the changes of MGEs. Multiple linear regression models also reveal the collective effect of these factors on ARGs, and the contributions of each factor impact on ARGs. This study provides more comprehension about the factors impact on the fate of ARGs during Pharmaceutical sludge treatment, and offers an approach to evaluate the importance of each factor, which method could be introduced for evaluation of factors influencing ARGs during other types of sludge or Wastewater treatment.

  • occurrence of antibiotic resistance genes and mobile genetic elements in enterococci and genomic dna during anaerobic digestion of Pharmaceutical Waste sludge with different pretreatments
    Bioresource Technology, 2017
    Co-Authors: Juan Tong, J Zhang, Qianwen Sui, Rui Wang, Meixue Chen, Yuansong Wei
    Abstract:

    Pharmaceutical Waste sludge harbors large amounts of antibiotic resistance genes (ARGs) and mobile genetic elements (MGEs), and it is necessary to study the reduction of ARGs and MGEs during sludge treatment. Therefore, the antibiotic resistance phenotypes and genotypes of enterococci, and the ARGs and MGEs in genomic DNA were investigated during anaerobic digestion (AD) with microwave (MW), thermal hydrolysis (TH) and ozone pretreatment. Results showed that sludge pretreatment increased the occurrence of the resistance phenotypes and genotypes of enterococci. During AD, the resistance of enterococci to macrolides decreased, except for in the MW-pretreated sludge. Horizontal gene transfer and co-occurrence of ermB and tetM in enterococci resulted in increased tetracycline resistance of enterococci throughout the sludge treatment. MGEs such as intI1, ISCR1 and Tn916/1545 had a significant effect on the distribution of ARGs. AD with pretreatment, especially TH pretreatment, resulted in greater ARGs and MGEs reduction and improved methane production.

Nikolina Udikovickolic - One of the best experts on this subject based on the ideXlab platform.

  • antibiotic manufacturing sites are hot spots for the release and spread of antibiotic resistance genes and mobile genetic elements in receiving aquatic environments
    Environment International, 2019
    Co-Authors: Juan Jose Gonzalezplaza, Khald Blau, Milena Milakovic, Tamara Jurina, Kornelia Smalla, Nikolina Udikovickolic
    Abstract:

    High antibiotic releases from manufacturing facilities have been identified as a risk factor for antibiotic resistance development in bacterial pathogens. However, the role of antibiotic pollution in selection and transferability of antibiotic resistance genes (ARGs) is still limited. In this study, we analyzed effluents from azithromycin-synthesis and veterinary-drug formulation facilities as well as sediments from receiving river and creek taken at the effluent discharge sites, upstream and downstream of discharge. Culturing showed that the effluent discharge significantly increased the proportion of antibiotic resistant bacteria in exposed sediments compared to the upstream ones. Quantitative real-time PCR revealed that effluents from both industries contained high and similar relative abundances of resistance genes [sul1, sul2, qacE/qacEΔ1, tet(A)], class 1 integrons (intI1) and IncP-1 plasmids (korB). Consequently, these genes significantly increased in relative abundances in receiving sediments, with more pronounced effects being observed for river than for creek sediments due to lower background levels of the investigated genes in the river. In addition, effluent discharge considerably increased transfer frequencies of captured ARGs from exposed sediments into Escherichia coli CV601 recipient as shown by biparental mating experiments. Most plasmids exogenously captured from effluent and polluted sediments belonged to the broad host range IncP-1e plasmid group, conferred multiple antibiotic resistance and harbored class 1 integrons. Discharge of Pharmaceutical Waste from antibiotic manufacturing sites thus poses a risk for development and dissemination of multi-resistant bacteria, including pathogens.

Hui Wang - One of the best experts on this subject based on the ideXlab platform.

  • comparison of ozone and thermal hydrolysis combined with anaerobic digestion for municipal and Pharmaceutical Waste sludge with tetracycline resistance genes
    Water Research, 2016
    Co-Authors: Hui Wang, Xiaohua Yu
    Abstract:

    Abstract Biosolids from Wastewater treatment plant (WWTP) are environmental reservoirs of antibiotic resistance genes, which attract great concerns on their efficient treatments. Anaerobic digestion (AD) is widely used for sewage sludge treatment but its effectiveness is limited due to the slow hydrolysis. Ozone and thermal hydrolysis pre-treatment were employed to improve AD efficiency and reduce antibiotic-resistant genes in municipal and Pharmaceutical Waste sludge (MWS and PWS, respectively) in this study. Sludge solubilization achieved 15.75–25.09% and 14.85–33.92% after ozone and thermal hydrolysis, respectively. Both pre-treatments improved cumulative methane production and the enhancements were greater on PWS than MWS. Five tetracycline-resistant genes ( tet (A), tet (G), tet (Q), tet (W), tet (X)) and one mobile element ( int I1) were qPCR to assess pre-treatments. AD of pre-treated sludge reduced more tet genes than raw sludge for both ozonation and thermal hydrolysis in PWS and MWS. Thermal hydrolysis pre-treatment was more efficient than ozone for reduction after AD. Results of this study help support management options for reducing the spread of antibiotic resistance from biosolids.

  • effect of ultrasonic and ozone pre treatments on Pharmaceutical Waste activated sludge s solubilisation reduction anaerobic biodegradability and acute biological toxicity
    Bioresource Technology, 2015
    Co-Authors: Jin Pei, Hong Yao, Hui Wang, Dan Shan, Yichen Jiang
    Abstract:

    Ultrasonic and ozone pre-treatment technologies were employed in this study to improve the anaerobic digestion efficiency of Pharmaceutical Waste activated sludge. The sludge solubilisation achieved 30.01% (150,000 kJ/kg TS) and 28.10% (0.1g O3/g TS) after ultrasonic treatment and ozone treatment. The anaerobic biodegradability after ultrasonic treatment was higher compared to ozonation due to the higher cumulative methane volume observed after 6 days (249 ml vs 190 ml). The ozonated sludge released the highest concentration of Cu(2+) into the liquid phase (6.640 mg L(-1)) compared to 0.530 mg/L for untreated sludge and 0.991 mg/L for sonicated sludge. The acute toxicity test measured by luminescent bacteria showed that anaerobic digestion could degrade toxic compounds and result in a reduction in toxicity. The main mechanism of action led to some differences in the treated sludge exhibiting higher potential for methane production from Pharmaceutical Waste sludge with ultrasonic treatment.