The Experts below are selected from a list of 390 Experts worldwide ranked by ideXlab platform
Yvette Michotte - One of the best experts on this subject based on the ideXlab platform.
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Development of a validated capillary electrophoresis method for enantiomeric purity testing of dexchlorPheniramine Maleate.
Journal of Chromatography A, 2002Co-Authors: Ann Van Eeckhaut, Marc Robert Detaevernier, Yvette MichotteAbstract:Abstract A capillary zone electrophoresis method has been developed for the detection of 0.1% of (R)-levochlorPheniramine Maleate in samples of (S)-dexchlorPheniramine Maleate. Using 1.5 mM carboxymethyl-β-cyclodextrin in an acidic background electrolyte, resolution values of more than 10 were obtained. Under these conditions the R-enantiomer is migrating in front of the bulk S-enantiomer. The assay was validated for linearity (2–10 μg/ml; R2=0.9992), selectivity [(RS)-Pheniramine Maleate and (RS)-bromPheniramine Maleate], limit of detection (0.25 μg/ml), limit of quantification (0.75 μg/ml), analytical precision (intra- and inter-day variability), repeatability of the method (RSD=5.0%) and accuracy. In samples of dexchlorPheniramine Maleate from two different manufacturers, concentrations of, respectively, 0.15% and 1.95% (m/m) of levochlorPheniramine Maleate were detected. The method was compared to the HPLC method described in the European Pharmacopoeia III monograph.
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Development of a validated capillary electrophoresis method for enantiomeric purity testing of dexchlorPheniramine Maleate.
Journal of Chromatography A, 2002Co-Authors: Ann Van Eeckhaut, Marc Robert Detaevernier, Yvette MichotteAbstract:A capillary zone electrophoresis method has been developed for the detection of 0.1% of (R)-levochlorPheniramine Maleate in samples of (S)-dexchlorPheniramine Maleate. Using 1.5 mM carboxymethyl-beta-cyclodextrin in an acidic background electrolyte, resolution values of more than 10 were obtained. Under these conditions the R-enantiomer is migrating in front of the bulk S-enantiomer. The assay was validated for linearity (2-10 microg/ml; R2 = 0.9992), selectivity [(RS)-Pheniramine Maleate and (RS)-bromPheniramine Maleate], limit of detection (0.25 microg/ml), limit of quantification (0.75 microg/ml), analytical precision (intra- and inter-day variability), repeatability of the method (RSD = 5.0%) and accuracy. In samples of dexchlorPheniramine Maleate from two different manufacturers, concentrations of, respectively, 0.15% and 1.95% (m/m) of levochlorPheniramine Maleate were detected. The method was compared to the HPLC method described in the European Pharmacopoeia III monograph.
Caglayan Osman - One of the best experts on this subject based on the ideXlab platform.
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In vitro antibacterial activity of some systemic and topical antihistaminic preparations
Canadian Soc Clinical Investigation, 2009Co-Authors: Gocmen, Julide Sedef, Buyukkocak Unase, Caglayan OsmanAbstract:WOS: 000207850100003PubMed: 20003827Purpose: In vitro antibacterial activity of topical and systemic antihistaminic preparations containing different active substrates against the standard strains of two bacteria was evaluated. Methods: Four topical and 3 systemic preparations containing Pheniramine Maleate, chlorophenoxamine hydrochloride, and diphenhydramine hydrochloride were studied. The antibacterial activities of these preparations against strains of S. aureus (American Type Culture Collection, ATCC 29213) and S. epidermidis (ATCC 25212) were tested using the disc diffusion method. In addition, the Minimal Innhibitory Concentration (MIC) and Minimal Bactericidal Concentration (MBC) of parenteral preparations for these two bacteria were determined. Results: Pheniramine Maleate-topical and Pheniramine Maleate-systemic had no activity against bacteria, but the others showed various rates of activity. Chlorophenoxamine hydrochloride-topical and chlorophenoxamine hydrochloride-systemic were the most effective (P < 0.05). Despite the same active substrate content, diphenhydramine hydrochloride-topical-1 and diphenhydramine hydrochloride-topical-2 yielded different results when they were compared with each other or with the other preparations. Diphenhydramine hydrochloride-topical-2 had a relatively higher rate of activity than diphenhydramine hydrochloride-topical-1. Inhibition zone diameters were 16.9 +/- 1.5 mm 12.3 +/- 0.5 mm for S. aureus, 17.4 +/- 1.0 mm 0 mm for S. epidermidis respectively (P < 0.05). MIC values of parenteral preparations were equal to or above 125 mu g/ml. Conclusion:, MIC values of parenteral preparations were higher than their blood levels in clinical use. Thus, effects of parenteral preparations may not have been reflected in routine clinical practice. However, topical forms have antibacterial activity due to additive substrates and the use of high concentration levels at the site of application. Therefore, in selection of topical forms for appropriate cases, these effects should also be taken into consideration. The antibacterial activity of topical antihistaminic preparations may be useful in certain dermatological pathology
Ann Van Eeckhaut - One of the best experts on this subject based on the ideXlab platform.
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Development of a validated capillary electrophoresis method for enantiomeric purity testing of dexchlorPheniramine Maleate.
Journal of Chromatography A, 2002Co-Authors: Ann Van Eeckhaut, Marc Robert Detaevernier, Yvette MichotteAbstract:Abstract A capillary zone electrophoresis method has been developed for the detection of 0.1% of (R)-levochlorPheniramine Maleate in samples of (S)-dexchlorPheniramine Maleate. Using 1.5 mM carboxymethyl-β-cyclodextrin in an acidic background electrolyte, resolution values of more than 10 were obtained. Under these conditions the R-enantiomer is migrating in front of the bulk S-enantiomer. The assay was validated for linearity (2–10 μg/ml; R2=0.9992), selectivity [(RS)-Pheniramine Maleate and (RS)-bromPheniramine Maleate], limit of detection (0.25 μg/ml), limit of quantification (0.75 μg/ml), analytical precision (intra- and inter-day variability), repeatability of the method (RSD=5.0%) and accuracy. In samples of dexchlorPheniramine Maleate from two different manufacturers, concentrations of, respectively, 0.15% and 1.95% (m/m) of levochlorPheniramine Maleate were detected. The method was compared to the HPLC method described in the European Pharmacopoeia III monograph.
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Development of a validated capillary electrophoresis method for enantiomeric purity testing of dexchlorPheniramine Maleate.
Journal of Chromatography A, 2002Co-Authors: Ann Van Eeckhaut, Marc Robert Detaevernier, Yvette MichotteAbstract:A capillary zone electrophoresis method has been developed for the detection of 0.1% of (R)-levochlorPheniramine Maleate in samples of (S)-dexchlorPheniramine Maleate. Using 1.5 mM carboxymethyl-beta-cyclodextrin in an acidic background electrolyte, resolution values of more than 10 were obtained. Under these conditions the R-enantiomer is migrating in front of the bulk S-enantiomer. The assay was validated for linearity (2-10 microg/ml; R2 = 0.9992), selectivity [(RS)-Pheniramine Maleate and (RS)-bromPheniramine Maleate], limit of detection (0.25 microg/ml), limit of quantification (0.75 microg/ml), analytical precision (intra- and inter-day variability), repeatability of the method (RSD = 5.0%) and accuracy. In samples of dexchlorPheniramine Maleate from two different manufacturers, concentrations of, respectively, 0.15% and 1.95% (m/m) of levochlorPheniramine Maleate were detected. The method was compared to the HPLC method described in the European Pharmacopoeia III monograph.
Osman Çağlayan - One of the best experts on this subject based on the ideXlab platform.
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In vitro antibacterial activity of some systemic and topical antihistaminic preparations.
Clinical & Investigative Medicine, 2009Co-Authors: Julide Sedef Göçmen, Ünase Büyükkoçak, Osman ÇağlayanAbstract:Purpose: In vitro antibacterial activity of topical and systemic antihistaminic preparations containing different active substrates against the standard strains of two bacteria was evaluated. Methods: Four topical and 3 systemic preparations containing Pheniramine Maleate, chlorophenoxamine hydrochloride, and diphenhydramine hydrochloride were studied. The antibacterial activities of these preparations against strains of S. aureus (American Type Culture Collection, ATCC 29213) and S. epidermidis (ATCC 25212) were tested using the disc diffusion method. In addition, the Minimal Innhibitory Concentration (MIC) and Minimal Bactericidal Concentration (MBC) of parenteral preparations for these two bacteria were determined. Results: Pheniramine Maleate-topical and Pheniramine Maleate-systemic had no activity against bacteria, but the others showed various rates of activity. Chlorophenoxamine hydrochloride-topical and chlorophenoxamine hydrochloride-systemic were the most effective (P < 0.05). Despite the same active substrate content, diphenhydramine hydrochloride-topical-1 and diphenhydramine hydrochloride-topical-2 yielded different results when they were compared with each other or with the other preparations. Diphenhydramine hydrochloride-topical-2 had a relatively higher rate of activity than diphenhydramine hydrochloride-topical-1. Inhibition zone diameters were 16.9±1.5 mm 12.3±0.5 mm for S .aureus, 17.4±1.0 mm 0 mm for S .epidermidis respectively (P < 0.05). MIC values of parenteral preparations were equal to or above 125 ?g/ml. Conclusion:, MIC values of parenteral preparations were higher than their blood levels in clinical use. Thus, effects of parenteral preparations may not have been reflected in routine clinical practice. However, topical forms have antibacterial activity due to additive substrates and the use of high concentration levels at the site of application. Therefore, in selection of topical forms for appropriate cases, these effects should also be taken into consideration. The antibacterial activity of topical antihistaminic preparations may be useful in certain dermatological pathology.
Jayasutha. J - One of the best experts on this subject based on the ideXlab platform.
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CASE REPOCASE REPORT ON METHOTREXATE-INDUCED ANGIOEDEMA, PANCYTOPENIA, AND STEVENS-JOHNSON SYNDROMERT ON METHOTREXATE INDUCED ANGIOEDEMA, PANCYTOPENIA AND STEVENS - JOHNSON SYNDROME
Asian Journal of Pharmaceutical and Clinical Research, 2016Co-Authors: Jayasutha. J, Sindhubharathi K.v, Sindhubharathi A, Sree Aakash KAbstract:ABSTRACT A 52-year-old female patient was presented with the complaints of swelling of lips, difficulty in breathing, difficulty in swallowing of food, itching, and facial puffiness and also showed the history of a skin lesion in the neck, forearm, and genitalia. Medical history of the patient showed that she had been prescribed with methotrexate for rheumatoid arthritis. Within 10 days of treatment, the patient developed above mentioned signs and symptoms. Further upon patient’s systemic and physical examinations were done and then a diagnosis of pancytopenia, angioedema, and StevensJohnson syndrome were made. According to Naranjo adverse drug reaction causality assessment scale, the association of angioedema, pancytopenia, and Stevens-Johnson syndrome due to methotrexate was probable. Methotrexate was withdrawn from the patient, and the patient was treated with methotrexate antagonist Leucovorin for 3 days with a frequency of thrice daily and injection Avil (Pheniramine Maleate) with a frequency of twice daily which resolved the complications of the patient. Keywords: Methotrexate, Angioedema, Pancytopenia, Stevens-Johnson syndrome.
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CASE REPORT ON METHOTREXATE-INDUCED ANGIOEDEMA, PANCYTOPENIA, AND STEVENS-JOHNSON SYNDROME
2016Co-Authors: Jayasutha. J, Vishnu Vandana GAbstract:A 52-year-old female patient was presented with the complaints of swelling of lips, difficulty in breathing, difficulty in swallowing of food, itching, and facial puffiness and also showed the history of a skin lesion in the neck, forearm, and genitalia. Medical history of the patient showed that she had been prescribed with methotrexate for rheumatoid arthritis. Within 10 days of treatment, the patient developed above mentioned signs and symptoms. Further upon patient’s systemic and physical examinations were done and then a diagnosis of pancytopenia, angioedema, and StevensJohnson syndrome were made. According to Naranjo adverse drug reaction causality assessment scale, the association of angioedema, pancytopenia, and Stevens-Johnson syndrome due to methotrexate was probable. Methotrexate was withdrawn from the patient, and the patient was treated with methotrexate antagonist Leucovorin for 3 days with a frequency of thrice daily and injection Avil (Pheniramine Maleate) with a frequency of twice daily which resolved the complications of the patient.