The Experts below are selected from a list of 54 Experts worldwide ranked by ideXlab platform

Seunggu Yeo - One of the best experts on this subject based on the ideXlab platform.

  • identification of hypoxanthine and Phosphoenolpyruvic Acid as serum markers associated with chemoradiotherapy response in locally advanced rectal cancer
    Journal of Clinical Oncology, 2014
    Co-Authors: Minjeong Kim, Byong Chul Yoo, Seunggu Yeo
    Abstract:

    507 Background: Patients show variable responses to chemoradiotherapy (CRT), generally performed before surgery for locally advanced rectal cancer (LARC). We aimed to identify molecular markers predictive of CRT responses by analyzing low-mass ions (LMIs) in serum of LARC patients. Methods: LMIs (<1000 m/z) in serum obtained before CRT from 73 LARC (cT3–4) patients were profiled using matrix-assisted laser desorption/ionization mass spectrometry. LMIs with higher weighting factors in discriminating CRT responses were selected using principal components analysis and discriminant analysis. Selected LMIs were identified using the Human Metabolome Database. The concentrations of identified LMIs were determined by colorimetric enzyme assay, and compared according to post-CRT pathological stage (ypStage) or Dworak’s tumor regression grade (TRG). Results: The nine highest-ranking LMIs were selected. Among them, two LMIs with 137.08 and 169.04 m/z were identified as hypoxanthine (HX) and Phosphoenolpyruvic Acid (...

Bin Liu - One of the best experts on this subject based on the ideXlab platform.

  • toward understanding the key enzymes involved in β poly l malic Acid biosynthesis by aureobasidium pullulans ipe 1
    Engineering in Life Sciences, 2018
    Co-Authors: Bin Liu, Jianquan Luo, Weifeng Cao, Changsheng Qiao, Yinhua Wan
    Abstract:

    β-poly (L-malic Acid) (PMLA) is a biopolyester which has attracted industrial interest for its potential application in medicine and other industries. A high dissolved oxygen concentration (DO) was beneficial for PMLA production, while the mechanisms of DO in PMLA biosynthesis by Aureobasidium pullulans are still poorly understood. In this work, the amount of PMLA was first compared when A. pullulans ipe-1 were cultured under a high DO level (70% saturation) and a low DO level (10% saturation). Meanwhile, the key enzymes involved in different pathways of the precursor L-malic Acid biosynthesis were studied. The results revealed that the activities of glucose-6-phosphate dehydrogenase (G6PDH) and phosphoenolpyruvate carboxylase (PEPC) were positively correlated with cell growth and PMLA production, while the activities of phosphofructokinases (PFK), pyruvic carboxylase (PC) and citrate synthetase (CS) did no show such correlations. It indicated that the Pentose Phosphate Pathway (PPP) may play a vital role in cell growth and PMLA biosynthesis. Moreover, the precursor L-malic Acid for PMLA biosynthesis was mainly biosynthesized through Phosphoenolpyruvic Acid (PEP) via oxaloacetate catalyzed by PEPC. It was also found that low concentration of sodium fluoride (NaF) might impel carbon flux flow to the oxaloacetate through PEP, but inhibit the flux to the oxaloacetate via pyruvic Acid.

Minjeong Kim - One of the best experts on this subject based on the ideXlab platform.

  • identification of hypoxanthine and Phosphoenolpyruvic Acid as serum markers associated with chemoradiotherapy response in locally advanced rectal cancer
    Journal of Clinical Oncology, 2014
    Co-Authors: Minjeong Kim, Byong Chul Yoo, Seunggu Yeo
    Abstract:

    507 Background: Patients show variable responses to chemoradiotherapy (CRT), generally performed before surgery for locally advanced rectal cancer (LARC). We aimed to identify molecular markers predictive of CRT responses by analyzing low-mass ions (LMIs) in serum of LARC patients. Methods: LMIs (<1000 m/z) in serum obtained before CRT from 73 LARC (cT3–4) patients were profiled using matrix-assisted laser desorption/ionization mass spectrometry. LMIs with higher weighting factors in discriminating CRT responses were selected using principal components analysis and discriminant analysis. Selected LMIs were identified using the Human Metabolome Database. The concentrations of identified LMIs were determined by colorimetric enzyme assay, and compared according to post-CRT pathological stage (ypStage) or Dworak’s tumor regression grade (TRG). Results: The nine highest-ranking LMIs were selected. Among them, two LMIs with 137.08 and 169.04 m/z were identified as hypoxanthine (HX) and Phosphoenolpyruvic Acid (...

Yinhua Wan - One of the best experts on this subject based on the ideXlab platform.

  • toward understanding the key enzymes involved in β poly l malic Acid biosynthesis by aureobasidium pullulans ipe 1
    Engineering in Life Sciences, 2018
    Co-Authors: Bin Liu, Jianquan Luo, Weifeng Cao, Changsheng Qiao, Yinhua Wan
    Abstract:

    β-poly (L-malic Acid) (PMLA) is a biopolyester which has attracted industrial interest for its potential application in medicine and other industries. A high dissolved oxygen concentration (DO) was beneficial for PMLA production, while the mechanisms of DO in PMLA biosynthesis by Aureobasidium pullulans are still poorly understood. In this work, the amount of PMLA was first compared when A. pullulans ipe-1 were cultured under a high DO level (70% saturation) and a low DO level (10% saturation). Meanwhile, the key enzymes involved in different pathways of the precursor L-malic Acid biosynthesis were studied. The results revealed that the activities of glucose-6-phosphate dehydrogenase (G6PDH) and phosphoenolpyruvate carboxylase (PEPC) were positively correlated with cell growth and PMLA production, while the activities of phosphofructokinases (PFK), pyruvic carboxylase (PC) and citrate synthetase (CS) did no show such correlations. It indicated that the Pentose Phosphate Pathway (PPP) may play a vital role in cell growth and PMLA biosynthesis. Moreover, the precursor L-malic Acid for PMLA biosynthesis was mainly biosynthesized through Phosphoenolpyruvic Acid (PEP) via oxaloacetate catalyzed by PEPC. It was also found that low concentration of sodium fluoride (NaF) might impel carbon flux flow to the oxaloacetate through PEP, but inhibit the flux to the oxaloacetate via pyruvic Acid.

Byong Chul Yoo - One of the best experts on this subject based on the ideXlab platform.

  • identification of hypoxanthine and Phosphoenolpyruvic Acid as serum markers associated with chemoradiotherapy response in locally advanced rectal cancer
    Journal of Clinical Oncology, 2014
    Co-Authors: Minjeong Kim, Byong Chul Yoo, Seunggu Yeo
    Abstract:

    507 Background: Patients show variable responses to chemoradiotherapy (CRT), generally performed before surgery for locally advanced rectal cancer (LARC). We aimed to identify molecular markers predictive of CRT responses by analyzing low-mass ions (LMIs) in serum of LARC patients. Methods: LMIs (<1000 m/z) in serum obtained before CRT from 73 LARC (cT3–4) patients were profiled using matrix-assisted laser desorption/ionization mass spectrometry. LMIs with higher weighting factors in discriminating CRT responses were selected using principal components analysis and discriminant analysis. Selected LMIs were identified using the Human Metabolome Database. The concentrations of identified LMIs were determined by colorimetric enzyme assay, and compared according to post-CRT pathological stage (ypStage) or Dworak’s tumor regression grade (TRG). Results: The nine highest-ranking LMIs were selected. Among them, two LMIs with 137.08 and 169.04 m/z were identified as hypoxanthine (HX) and Phosphoenolpyruvic Acid (...