The Experts below are selected from a list of 138 Experts worldwide ranked by ideXlab platform
Thomas Czerny - One of the best experts on this subject based on the ideXlab platform.
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The function of tcf3 in medaka embryos: efficient knockdown with pePNAs
BMC Biotechnology, 2018Co-Authors: Gerlinde Doenz, Sebastian Dorn, Narges Aghaallaei, Baubak Bajoghli, Birgit Werner, Holger Bock, Thomas Lindhorst, Elisabeth Riegel, Michaela Aigner, Thomas CzernyAbstract:Background The application of antisense molecules, such as morpholino oligonucleotides, is an efficient method of gene inactivation in vivo. We recently introduced Phosphonic Ester modified peptide nucleic acids (PNA) for in vivo loss-of-function experiments in medaka embryos. Here we tested novel modifications of the PNA backbone to knockdown the medaka tcf3 gene. Results A single tcf3 gene exists in the medaka genome and its inactivation strongly affected eye development of the embryos, leading to size reduction and anophthalmia in severe cases. The function of Tcf3 strongly depends on co-repressor interactions. We found interactions with Groucho/Tle proteins to be most important for eye development. Using a dominant negative approach for combined inactivation of all groucho/tle genes also resulted in eye phenotypes, as did interference with three individual tle genes. Conclusions Our results show that side chain modified PNAs come close to the knockdown efficiency of morpholino oligonucleotides in vivo. A single medaka tcf3 gene combines the function of the two zebrafish paralogs hdl and tcf3b . In combination with Groucho/Tle corepressor proteins Tcf3 acts in anterior development and is critical for eye formation.
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The function of tcf3 in medaka embryos: efficient knockdown with pePNAs.
BMC Biotechnology, 2018Co-Authors: Gerlinde Doenz, Sebastian Dorn, Narges Aghaallaei, Baubak Bajoghli, Birgit Werner, Holger Bock, Thomas Lindhorst, Elisabeth Riegel, Michaela Aigner, Thomas CzernyAbstract:The application of antisense molecules, such as morpholino oligonucleotides, is an efficient method of gene inactivation in vivo. We recently introduced Phosphonic Ester modified peptide nucleic acids (PNA) for in vivo loss-of-function experiments in medaka embryos. Here we tested novel modifications of the PNA backbone to knockdown the medaka tcf3 gene. A single tcf3 gene exists in the medaka genome and its inactivation strongly affected eye development of the embryos, leading to size reduction and anophthalmia in severe cases. The function of Tcf3 strongly depends on co-repressor interactions. We found interactions with Groucho/Tle proteins to be most important for eye development. Using a dominant negative approach for combined inactivation of all groucho/tle genes also resulted in eye phenotypes, as did interference with three individual tle genes. Our results show that side chain modified PNAs come close to the knockdown efficiency of morpholino oligonucleotides in vivo. A single medaka tcf3 gene combines the function of the two zebrafish paralogs hdl and tcf3b. In combination with Groucho/Tle corepressor proteins Tcf3 acts in anterior development and is critical for eye formation.
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Side chain modified peptide nucleic acids (PNA) for knock-down of six3 in medaka embryos
BMC Biotechnology, 2012Co-Authors: Sebastian Dorn, Narges Aghaallaei, Gerlinde Jung, Baubak Bajoghli, Birgit Werner, Holger Bock, Thomas Lindhorst, Thomas CzernyAbstract:Background: Synthetic antisense molecules have an enormous potential for therapeutic applications in humans. The major aim of such strategies is to specifically interfere with gene function, thus modulating cellular pathways according to the therapeutic demands. Among the molecules which can block mRNA function in a sequence specific manner are peptide nucleic acids (PNA). They are highly stable and efficiently and selectively interact with RNA. However, some properties of non-modified aminoethyl glycine PNAs (aegPNA) hamper their in vivo applications. Results: We generated new backbone modifications of PNAs, which exhibit more hydrophilic properties. When we examined the activity and specificity of these novel Phosphonic Ester PNAs (pePNA) molecules in medaka (Oryzias latipes) embryos, high solubility and selective binding to mRNA was observed. In particular, mixing of the novel components with aegPNA components resulted in mixed PNAs with superior properties. Injection of mixed PNAs directed against the medaka six3 gene, which is important for eye and brain development, resulted in specific six3 phenotypes. Conclusions: PNAs are well established as powerful antisense molecules. Modification of the backbone with Phosphonic Ester side chains further improves their properties and allows the efficient knock down of a single gene in fish embryos.
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Side chain modified peptide nucleic acids (PNA) for knock-down of six3in medaka embryos
BMC Biotechnology, 2012Co-Authors: Sebastian Dorn, Narges Aghaallaei, Gerlinde Jung, Baubak Bajoghli, Birgit Werner, Holger Bock, Thomas Lindhorst, Thomas CzernyAbstract:Background Synthetic antisense molecules have an enormous potential for therapeutic applications in humans. The major aim of such strategies is to specifically interfere with gene function, thus modulating cellular pathways according to the therapeutic demands. Among the molecules which can block mRNA function in a sequence specific manner are peptide nucleic acids (PNA). They are highly stable and efficiently and selectively interact with RNA. However, some properties of non-modified aminoethyl glycine PNAs (aegPNA) hamper their in vivo applications. Results We generated new backbone modifications of PNAs, which exhibit more hydrophilic properties. When we examined the activity and specificity of these novel Phosphonic Ester PNAs (pePNA) molecules in medaka ( Oryzias latipes ) embryos, high solubility and selective binding to mRNA was observed. In particular, mixing of the novel components with aegPNA components resulted in mixed PNAs with superior properties. Injection of mixed PNAs directed against the medaka six3 gene, which is important for eye and brain development, resulted in specific six3 phenotypes. Conclusions PNAs are well established as powerful antisense molecules. Modification of the backbone with Phosphonic Ester side chains further improves their properties and allows the efficient knock down of a single gene in fish embryos.
Paul O Biney - One of the best experts on this subject based on the ideXlab platform.
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dispersion of aminoalkylsilyl Ester or amine alkyl Phosphonic acid side wall functionalized carbon nanotubes in silica using sol gel processing
Materials Letters, 2008Co-Authors: Luqman A Adams, Valery N Khabashesku, Yamen Edigin, Paul O BineyAbstract:Aminopropyltriethoxysilane and aminopropyl-Phosphonic Ester modified carbon nanotubes were prepared by reacting fluorinated carbon nanotubes (F-CNTs) with the 3-aminopropyltriethoxysilane or 3-aminopropyl-Phosphonic acid reagents at 120 °C temperature, using pyridine as the base catalyst. These functionalized carbon nanotubes, APTES-CNTs, 1, and APPA-CNTs, 2, were characterized by transmission electron microscopy (TEM), infrared spectroscopy (IR), and thermogravimetric analysis (TGA). The homogeneous dispersion of these functionalized CNTs (0.1%) in silica were also accomplished by sol-gel processing. The TEM confirmed uniform dispersion of the functionalized CNT in silica.
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Dispersion of aminoalkylsilyl Ester or amine alkyl-Phosphonic acid side wall functionalized carbon nanotubes in silica using sol–gel processing
Materials Letters, 2007Co-Authors: Aderemi Oki, Luqman A Adams, Valery N Khabashesku, Yamen Edigin, Paul O Biney, Zhiping LuoAbstract:Aminopropyltriethoxysilane and aminopropyl-Phosphonic Ester modified carbon nanotubes were prepared by reacting fluorinated carbon nanotubes (F-CNTs) with the 3-aminopropyltriethoxysilane or 3-aminopropyl-Phosphonic acid reagents at 120 °C temperature, using pyridine as the base catalyst. These functionalized carbon nanotubes, APTES-CNTs, 1, and APPA-CNTs, 2, were characterized by transmission electron microscopy (TEM), infrared spectroscopy (IR), and thermogravimetric analysis (TGA). The homogeneous dispersion of these functionalized CNTs (0.1%) in silica were also accomplished by sol-gel processing. The TEM confirmed uniform dispersion of the functionalized CNT in silica.
Valery N Khabashesku - One of the best experts on this subject based on the ideXlab platform.
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dispersion of aminoalkylsilyl Ester or amine alkyl Phosphonic acid side wall functionalized carbon nanotubes in silica using sol gel processing
Materials Letters, 2008Co-Authors: Luqman A Adams, Valery N Khabashesku, Yamen Edigin, Paul O BineyAbstract:Aminopropyltriethoxysilane and aminopropyl-Phosphonic Ester modified carbon nanotubes were prepared by reacting fluorinated carbon nanotubes (F-CNTs) with the 3-aminopropyltriethoxysilane or 3-aminopropyl-Phosphonic acid reagents at 120 °C temperature, using pyridine as the base catalyst. These functionalized carbon nanotubes, APTES-CNTs, 1, and APPA-CNTs, 2, were characterized by transmission electron microscopy (TEM), infrared spectroscopy (IR), and thermogravimetric analysis (TGA). The homogeneous dispersion of these functionalized CNTs (0.1%) in silica were also accomplished by sol-gel processing. The TEM confirmed uniform dispersion of the functionalized CNT in silica.
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Dispersion of aminoalkylsilyl Ester or amine alkyl-Phosphonic acid side wall functionalized carbon nanotubes in silica using sol–gel processing
Materials Letters, 2007Co-Authors: Aderemi Oki, Luqman A Adams, Valery N Khabashesku, Yamen Edigin, Paul O Biney, Zhiping LuoAbstract:Aminopropyltriethoxysilane and aminopropyl-Phosphonic Ester modified carbon nanotubes were prepared by reacting fluorinated carbon nanotubes (F-CNTs) with the 3-aminopropyltriethoxysilane or 3-aminopropyl-Phosphonic acid reagents at 120 °C temperature, using pyridine as the base catalyst. These functionalized carbon nanotubes, APTES-CNTs, 1, and APPA-CNTs, 2, were characterized by transmission electron microscopy (TEM), infrared spectroscopy (IR), and thermogravimetric analysis (TGA). The homogeneous dispersion of these functionalized CNTs (0.1%) in silica were also accomplished by sol-gel processing. The TEM confirmed uniform dispersion of the functionalized CNT in silica.
Sebastian Dorn - One of the best experts on this subject based on the ideXlab platform.
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The function of tcf3 in medaka embryos: efficient knockdown with pePNAs
BMC Biotechnology, 2018Co-Authors: Gerlinde Doenz, Sebastian Dorn, Narges Aghaallaei, Baubak Bajoghli, Birgit Werner, Holger Bock, Thomas Lindhorst, Elisabeth Riegel, Michaela Aigner, Thomas CzernyAbstract:Background The application of antisense molecules, such as morpholino oligonucleotides, is an efficient method of gene inactivation in vivo. We recently introduced Phosphonic Ester modified peptide nucleic acids (PNA) for in vivo loss-of-function experiments in medaka embryos. Here we tested novel modifications of the PNA backbone to knockdown the medaka tcf3 gene. Results A single tcf3 gene exists in the medaka genome and its inactivation strongly affected eye development of the embryos, leading to size reduction and anophthalmia in severe cases. The function of Tcf3 strongly depends on co-repressor interactions. We found interactions with Groucho/Tle proteins to be most important for eye development. Using a dominant negative approach for combined inactivation of all groucho/tle genes also resulted in eye phenotypes, as did interference with three individual tle genes. Conclusions Our results show that side chain modified PNAs come close to the knockdown efficiency of morpholino oligonucleotides in vivo. A single medaka tcf3 gene combines the function of the two zebrafish paralogs hdl and tcf3b . In combination with Groucho/Tle corepressor proteins Tcf3 acts in anterior development and is critical for eye formation.
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The function of tcf3 in medaka embryos: efficient knockdown with pePNAs.
BMC Biotechnology, 2018Co-Authors: Gerlinde Doenz, Sebastian Dorn, Narges Aghaallaei, Baubak Bajoghli, Birgit Werner, Holger Bock, Thomas Lindhorst, Elisabeth Riegel, Michaela Aigner, Thomas CzernyAbstract:The application of antisense molecules, such as morpholino oligonucleotides, is an efficient method of gene inactivation in vivo. We recently introduced Phosphonic Ester modified peptide nucleic acids (PNA) for in vivo loss-of-function experiments in medaka embryos. Here we tested novel modifications of the PNA backbone to knockdown the medaka tcf3 gene. A single tcf3 gene exists in the medaka genome and its inactivation strongly affected eye development of the embryos, leading to size reduction and anophthalmia in severe cases. The function of Tcf3 strongly depends on co-repressor interactions. We found interactions with Groucho/Tle proteins to be most important for eye development. Using a dominant negative approach for combined inactivation of all groucho/tle genes also resulted in eye phenotypes, as did interference with three individual tle genes. Our results show that side chain modified PNAs come close to the knockdown efficiency of morpholino oligonucleotides in vivo. A single medaka tcf3 gene combines the function of the two zebrafish paralogs hdl and tcf3b. In combination with Groucho/Tle corepressor proteins Tcf3 acts in anterior development and is critical for eye formation.
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Side chain modified peptide nucleic acids (PNA) for knock-down of six3 in medaka embryos
BMC Biotechnology, 2012Co-Authors: Sebastian Dorn, Narges Aghaallaei, Gerlinde Jung, Baubak Bajoghli, Birgit Werner, Holger Bock, Thomas Lindhorst, Thomas CzernyAbstract:Background: Synthetic antisense molecules have an enormous potential for therapeutic applications in humans. The major aim of such strategies is to specifically interfere with gene function, thus modulating cellular pathways according to the therapeutic demands. Among the molecules which can block mRNA function in a sequence specific manner are peptide nucleic acids (PNA). They are highly stable and efficiently and selectively interact with RNA. However, some properties of non-modified aminoethyl glycine PNAs (aegPNA) hamper their in vivo applications. Results: We generated new backbone modifications of PNAs, which exhibit more hydrophilic properties. When we examined the activity and specificity of these novel Phosphonic Ester PNAs (pePNA) molecules in medaka (Oryzias latipes) embryos, high solubility and selective binding to mRNA was observed. In particular, mixing of the novel components with aegPNA components resulted in mixed PNAs with superior properties. Injection of mixed PNAs directed against the medaka six3 gene, which is important for eye and brain development, resulted in specific six3 phenotypes. Conclusions: PNAs are well established as powerful antisense molecules. Modification of the backbone with Phosphonic Ester side chains further improves their properties and allows the efficient knock down of a single gene in fish embryos.
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Side chain modified peptide nucleic acids (PNA) for knock-down of six3in medaka embryos
BMC Biotechnology, 2012Co-Authors: Sebastian Dorn, Narges Aghaallaei, Gerlinde Jung, Baubak Bajoghli, Birgit Werner, Holger Bock, Thomas Lindhorst, Thomas CzernyAbstract:Background Synthetic antisense molecules have an enormous potential for therapeutic applications in humans. The major aim of such strategies is to specifically interfere with gene function, thus modulating cellular pathways according to the therapeutic demands. Among the molecules which can block mRNA function in a sequence specific manner are peptide nucleic acids (PNA). They are highly stable and efficiently and selectively interact with RNA. However, some properties of non-modified aminoethyl glycine PNAs (aegPNA) hamper their in vivo applications. Results We generated new backbone modifications of PNAs, which exhibit more hydrophilic properties. When we examined the activity and specificity of these novel Phosphonic Ester PNAs (pePNA) molecules in medaka ( Oryzias latipes ) embryos, high solubility and selective binding to mRNA was observed. In particular, mixing of the novel components with aegPNA components resulted in mixed PNAs with superior properties. Injection of mixed PNAs directed against the medaka six3 gene, which is important for eye and brain development, resulted in specific six3 phenotypes. Conclusions PNAs are well established as powerful antisense molecules. Modification of the backbone with Phosphonic Ester side chains further improves their properties and allows the efficient knock down of a single gene in fish embryos.
Luqman A Adams - One of the best experts on this subject based on the ideXlab platform.
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dispersion of aminoalkylsilyl Ester or amine alkyl Phosphonic acid side wall functionalized carbon nanotubes in silica using sol gel processing
Materials Letters, 2008Co-Authors: Luqman A Adams, Valery N Khabashesku, Yamen Edigin, Paul O BineyAbstract:Aminopropyltriethoxysilane and aminopropyl-Phosphonic Ester modified carbon nanotubes were prepared by reacting fluorinated carbon nanotubes (F-CNTs) with the 3-aminopropyltriethoxysilane or 3-aminopropyl-Phosphonic acid reagents at 120 °C temperature, using pyridine as the base catalyst. These functionalized carbon nanotubes, APTES-CNTs, 1, and APPA-CNTs, 2, were characterized by transmission electron microscopy (TEM), infrared spectroscopy (IR), and thermogravimetric analysis (TGA). The homogeneous dispersion of these functionalized CNTs (0.1%) in silica were also accomplished by sol-gel processing. The TEM confirmed uniform dispersion of the functionalized CNT in silica.
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Dispersion of aminoalkylsilyl Ester or amine alkyl-Phosphonic acid side wall functionalized carbon nanotubes in silica using sol–gel processing
Materials Letters, 2007Co-Authors: Aderemi Oki, Luqman A Adams, Valery N Khabashesku, Yamen Edigin, Paul O Biney, Zhiping LuoAbstract:Aminopropyltriethoxysilane and aminopropyl-Phosphonic Ester modified carbon nanotubes were prepared by reacting fluorinated carbon nanotubes (F-CNTs) with the 3-aminopropyltriethoxysilane or 3-aminopropyl-Phosphonic acid reagents at 120 °C temperature, using pyridine as the base catalyst. These functionalized carbon nanotubes, APTES-CNTs, 1, and APPA-CNTs, 2, were characterized by transmission electron microscopy (TEM), infrared spectroscopy (IR), and thermogravimetric analysis (TGA). The homogeneous dispersion of these functionalized CNTs (0.1%) in silica were also accomplished by sol-gel processing. The TEM confirmed uniform dispersion of the functionalized CNT in silica.