The Experts below are selected from a list of 282 Experts worldwide ranked by ideXlab platform
Toshiaki Sekine - One of the best experts on this subject based on the ideXlab platform.
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input output analysis of in vivo Photoassimilate translocation using positron emitting tracer imaging system petis data
Journal of Experimental Botany, 2005Co-Authors: Anna J. Keutgen, Norbert Keutgen, Shinpei Matsuhashi, Chizuko Mizuniwa, Takehito Ito, Takashi Fujimura, N. S. Ishioka, Satoshi Watanabe, Akihiko Osa, Toshiaki SekineAbstract:The Positron-Emitting Tracer Imaging System (PETIS) is introduced for monitoring the distribution of C-11-labelled Photoassimilates in Sorghum. The obtained two-dimensional image data were quantitatively analysed using a transfer function analysis approach. While one half of a Sorghum root in a split root system was treated with either 0, 100, or 500 mM NaCl dissolved in the nutrient solution, tracer images of the root halves and the lower stem section were recorded using PETIS. From the observed tracer levels, parameters were estimated, from which the mean speed of tracer transport and the proportion of tracer moved between specified image positions were deduced. Transport speed varied between 0.7 and 1.8 cm min(-1) with the difference depending on which part of the stem was involved. When data were collected in the lowest 0.5-1 cm of the stem, which included the point where the roots emerge, transport speed was less. Rapid changes in NaCl concentration, from 0 to 100 mM, resulted in short-term increases of assimilate import into the treated root. This response represented a transient osmotic effect, that was compensated for in the medium-term by osmotic adaptation. Higher concentrations of NaCl (500 mM) resulted in distinctly less Photoassimilate transport into the treated root half. The present results agree with earlier observations, showing that transport of C-11-labelled Photoassimilates measured with the PETIS detector system can be quantified using the method of input-output analysis. It is worth noting that with the PETIS detector system, areas of interest do not need to be defined until after data collection. This means that unexpected behaviour of a plant organ will be seen, which is not necessarily the case with conventional detector systems looking at predefined areas of interest.
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Input–output analysis of in vivo Photoassimilate translocation using Positron-Emitting Tracer Imaging System (PETIS) data
Journal of experimental botany, 2005Co-Authors: Anna J. Keutgen, Norbert Keutgen, Shinpei Matsuhashi, Chizuko Mizuniwa, Takehito Ito, Takashi Fujimura, N. S. Ishioka, Satoshi Watanabe, Akihiko Osa, Toshiaki SekineAbstract:The Positron-Emitting Tracer Imaging System (PETIS) is introduced for monitoring the distribution of C-11-labelled Photoassimilates in Sorghum. The obtained two-dimensional image data were quantitatively analysed using a transfer function analysis approach. While one half of a Sorghum root in a split root system was treated with either 0, 100, or 500 mM NaCl dissolved in the nutrient solution, tracer images of the root halves and the lower stem section were recorded using PETIS. From the observed tracer levels, parameters were estimated, from which the mean speed of tracer transport and the proportion of tracer moved between specified image positions were deduced. Transport speed varied between 0.7 and 1.8 cm min(-1) with the difference depending on which part of the stem was involved. When data were collected in the lowest 0.5-1 cm of the stem, which included the point where the roots emerge, transport speed was less. Rapid changes in NaCl concentration, from 0 to 100 mM, resulted in short-term increases of assimilate import into the treated root. This response represented a transient osmotic effect, that was compensated for in the medium-term by osmotic adaptation. Higher concentrations of NaCl (500 mM) resulted in distinctly less Photoassimilate transport into the treated root half. The present results agree with earlier observations, showing that transport of C-11-labelled Photoassimilates measured with the PETIS detector system can be quantified using the method of input-output analysis. It is worth noting that with the PETIS detector system, areas of interest do not need to be defined until after data collection. This means that unexpected behaviour of a plant organ will be seen, which is not necessarily the case with conventional detector systems looking at predefined areas of interest.
Anna J. Keutgen - One of the best experts on this subject based on the ideXlab platform.
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input output analysis of in vivo Photoassimilate translocation using positron emitting tracer imaging system petis data
Journal of Experimental Botany, 2005Co-Authors: Anna J. Keutgen, Norbert Keutgen, Shinpei Matsuhashi, Chizuko Mizuniwa, Takehito Ito, Takashi Fujimura, N. S. Ishioka, Satoshi Watanabe, Akihiko Osa, Toshiaki SekineAbstract:The Positron-Emitting Tracer Imaging System (PETIS) is introduced for monitoring the distribution of C-11-labelled Photoassimilates in Sorghum. The obtained two-dimensional image data were quantitatively analysed using a transfer function analysis approach. While one half of a Sorghum root in a split root system was treated with either 0, 100, or 500 mM NaCl dissolved in the nutrient solution, tracer images of the root halves and the lower stem section were recorded using PETIS. From the observed tracer levels, parameters were estimated, from which the mean speed of tracer transport and the proportion of tracer moved between specified image positions were deduced. Transport speed varied between 0.7 and 1.8 cm min(-1) with the difference depending on which part of the stem was involved. When data were collected in the lowest 0.5-1 cm of the stem, which included the point where the roots emerge, transport speed was less. Rapid changes in NaCl concentration, from 0 to 100 mM, resulted in short-term increases of assimilate import into the treated root. This response represented a transient osmotic effect, that was compensated for in the medium-term by osmotic adaptation. Higher concentrations of NaCl (500 mM) resulted in distinctly less Photoassimilate transport into the treated root half. The present results agree with earlier observations, showing that transport of C-11-labelled Photoassimilates measured with the PETIS detector system can be quantified using the method of input-output analysis. It is worth noting that with the PETIS detector system, areas of interest do not need to be defined until after data collection. This means that unexpected behaviour of a plant organ will be seen, which is not necessarily the case with conventional detector systems looking at predefined areas of interest.
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Input–output analysis of in vivo Photoassimilate translocation using Positron-Emitting Tracer Imaging System (PETIS) data
Journal of experimental botany, 2005Co-Authors: Anna J. Keutgen, Norbert Keutgen, Shinpei Matsuhashi, Chizuko Mizuniwa, Takehito Ito, Takashi Fujimura, N. S. Ishioka, Satoshi Watanabe, Akihiko Osa, Toshiaki SekineAbstract:The Positron-Emitting Tracer Imaging System (PETIS) is introduced for monitoring the distribution of C-11-labelled Photoassimilates in Sorghum. The obtained two-dimensional image data were quantitatively analysed using a transfer function analysis approach. While one half of a Sorghum root in a split root system was treated with either 0, 100, or 500 mM NaCl dissolved in the nutrient solution, tracer images of the root halves and the lower stem section were recorded using PETIS. From the observed tracer levels, parameters were estimated, from which the mean speed of tracer transport and the proportion of tracer moved between specified image positions were deduced. Transport speed varied between 0.7 and 1.8 cm min(-1) with the difference depending on which part of the stem was involved. When data were collected in the lowest 0.5-1 cm of the stem, which included the point where the roots emerge, transport speed was less. Rapid changes in NaCl concentration, from 0 to 100 mM, resulted in short-term increases of assimilate import into the treated root. This response represented a transient osmotic effect, that was compensated for in the medium-term by osmotic adaptation. Higher concentrations of NaCl (500 mM) resulted in distinctly less Photoassimilate transport into the treated root half. The present results agree with earlier observations, showing that transport of C-11-labelled Photoassimilates measured with the PETIS detector system can be quantified using the method of input-output analysis. It is worth noting that with the PETIS detector system, areas of interest do not need to be defined until after data collection. This means that unexpected behaviour of a plant organ will be seen, which is not necessarily the case with conventional detector systems looking at predefined areas of interest.
Shinpei Matsuhashi - One of the best experts on this subject based on the ideXlab platform.
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A new visualization technique for the study of the accumulation of Photoassimilates in wheat grains using [11C]CO2
Applied radiation and isotopes : including data instrumentation and methods for use in agriculture industry and medicine, 2005Co-Authors: Shinpei Matsuhashi, Shu Fujimaki, Noriko S. Ishioka, Hiroshi Uchida, Tamikazu KumeAbstract:Abstract Non-invasive real-time visualization of the accumulation of Photoassimilates in the grains of an ear of wheat using [ 11 C]CO 2 and positron emitting tracer imaging system (PETIS) was studied. [ 11 C]CO 2 was supplied to the center of a fully expanded leaf of a wheat plant for an initial 10 min, and the transportation of 11 C-labeled Photoassimilates into the grains of the ear was monitored for 120 min using the PETIS. Each grain was clearly identified in the obtained animation. The 11 C-labeled Photoassimilates arrived at the ear from the [ 11 C]CO 2 -absorbing leaf within 53 min from the time of supplying [ 11 C]CO 2 . After that, grains appeared on the image one by one from the basal part and full images of the grains appeared within 20 min. The time course of the accumulation of Photoassimilates into each grain showed a different profile. Furthermore, the PETIS data suggested that the photo-condition of the ear plays an important role in the transportation of Photoassimilates in wheat. PETIS can be used to visualize the dynamics of the substances in a living plant in real time and can exhibit the time course analysis of substances, such as the transportation, distribution, and accumulation.
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input output analysis of in vivo Photoassimilate translocation using positron emitting tracer imaging system petis data
Journal of Experimental Botany, 2005Co-Authors: Anna J. Keutgen, Norbert Keutgen, Shinpei Matsuhashi, Chizuko Mizuniwa, Takehito Ito, Takashi Fujimura, N. S. Ishioka, Satoshi Watanabe, Akihiko Osa, Toshiaki SekineAbstract:The Positron-Emitting Tracer Imaging System (PETIS) is introduced for monitoring the distribution of C-11-labelled Photoassimilates in Sorghum. The obtained two-dimensional image data were quantitatively analysed using a transfer function analysis approach. While one half of a Sorghum root in a split root system was treated with either 0, 100, or 500 mM NaCl dissolved in the nutrient solution, tracer images of the root halves and the lower stem section were recorded using PETIS. From the observed tracer levels, parameters were estimated, from which the mean speed of tracer transport and the proportion of tracer moved between specified image positions were deduced. Transport speed varied between 0.7 and 1.8 cm min(-1) with the difference depending on which part of the stem was involved. When data were collected in the lowest 0.5-1 cm of the stem, which included the point where the roots emerge, transport speed was less. Rapid changes in NaCl concentration, from 0 to 100 mM, resulted in short-term increases of assimilate import into the treated root. This response represented a transient osmotic effect, that was compensated for in the medium-term by osmotic adaptation. Higher concentrations of NaCl (500 mM) resulted in distinctly less Photoassimilate transport into the treated root half. The present results agree with earlier observations, showing that transport of C-11-labelled Photoassimilates measured with the PETIS detector system can be quantified using the method of input-output analysis. It is worth noting that with the PETIS detector system, areas of interest do not need to be defined until after data collection. This means that unexpected behaviour of a plant organ will be seen, which is not necessarily the case with conventional detector systems looking at predefined areas of interest.
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Input–output analysis of in vivo Photoassimilate translocation using Positron-Emitting Tracer Imaging System (PETIS) data
Journal of experimental botany, 2005Co-Authors: Anna J. Keutgen, Norbert Keutgen, Shinpei Matsuhashi, Chizuko Mizuniwa, Takehito Ito, Takashi Fujimura, N. S. Ishioka, Satoshi Watanabe, Akihiko Osa, Toshiaki SekineAbstract:The Positron-Emitting Tracer Imaging System (PETIS) is introduced for monitoring the distribution of C-11-labelled Photoassimilates in Sorghum. The obtained two-dimensional image data were quantitatively analysed using a transfer function analysis approach. While one half of a Sorghum root in a split root system was treated with either 0, 100, or 500 mM NaCl dissolved in the nutrient solution, tracer images of the root halves and the lower stem section were recorded using PETIS. From the observed tracer levels, parameters were estimated, from which the mean speed of tracer transport and the proportion of tracer moved between specified image positions were deduced. Transport speed varied between 0.7 and 1.8 cm min(-1) with the difference depending on which part of the stem was involved. When data were collected in the lowest 0.5-1 cm of the stem, which included the point where the roots emerge, transport speed was less. Rapid changes in NaCl concentration, from 0 to 100 mM, resulted in short-term increases of assimilate import into the treated root. This response represented a transient osmotic effect, that was compensated for in the medium-term by osmotic adaptation. Higher concentrations of NaCl (500 mM) resulted in distinctly less Photoassimilate transport into the treated root half. The present results agree with earlier observations, showing that transport of C-11-labelled Photoassimilates measured with the PETIS detector system can be quantified using the method of input-output analysis. It is worth noting that with the PETIS detector system, areas of interest do not need to be defined until after data collection. This means that unexpected behaviour of a plant organ will be seen, which is not necessarily the case with conventional detector systems looking at predefined areas of interest.
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Quantitative Modeling of Photoassimilate Flow in an Intact Plant Using the Positron Emitting Tracer Imaging System (PETIS)
Soil Science and Plant Nutrition, 2005Co-Authors: Shinpei Matsuhashi, Shu Fujimaki, Naoki Kawachi, Koichi Sakamoto, Noriko S. Ishioka, Tamikazu KumeAbstract:The Photoassimilate flow in an intact plant stem was imaged in real-time and its dynamics was quantitatively described using the Positron Emitting Tracer Imaging System (PETIS). Radioactive 11CO2 was fed to a leaf of an intact broad bean (Vicia faba L.) plant, together with air containing an ambient concentration of non-radioactive carrier CO2 gas. Movies of flow of the 11C-labeled Photoassimilates in the plant body were captured with PETIS. Here we demonstrate that the average flow speeds and the distribution ratios of Photoassimilates in the respective nodes and internodes of the observed stem can be estimated by the transfer function analysis, one of the mathematical modeling methods. We also estimated the changes in the spatial distribution of the average flow speeds in the same stem when the fed leaf was exposed to enriched carrier CO2 gas.
Akihiko Osa - One of the best experts on this subject based on the ideXlab platform.
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input output analysis of in vivo Photoassimilate translocation using positron emitting tracer imaging system petis data
Journal of Experimental Botany, 2005Co-Authors: Anna J. Keutgen, Norbert Keutgen, Shinpei Matsuhashi, Chizuko Mizuniwa, Takehito Ito, Takashi Fujimura, N. S. Ishioka, Satoshi Watanabe, Akihiko Osa, Toshiaki SekineAbstract:The Positron-Emitting Tracer Imaging System (PETIS) is introduced for monitoring the distribution of C-11-labelled Photoassimilates in Sorghum. The obtained two-dimensional image data were quantitatively analysed using a transfer function analysis approach. While one half of a Sorghum root in a split root system was treated with either 0, 100, or 500 mM NaCl dissolved in the nutrient solution, tracer images of the root halves and the lower stem section were recorded using PETIS. From the observed tracer levels, parameters were estimated, from which the mean speed of tracer transport and the proportion of tracer moved between specified image positions were deduced. Transport speed varied between 0.7 and 1.8 cm min(-1) with the difference depending on which part of the stem was involved. When data were collected in the lowest 0.5-1 cm of the stem, which included the point where the roots emerge, transport speed was less. Rapid changes in NaCl concentration, from 0 to 100 mM, resulted in short-term increases of assimilate import into the treated root. This response represented a transient osmotic effect, that was compensated for in the medium-term by osmotic adaptation. Higher concentrations of NaCl (500 mM) resulted in distinctly less Photoassimilate transport into the treated root half. The present results agree with earlier observations, showing that transport of C-11-labelled Photoassimilates measured with the PETIS detector system can be quantified using the method of input-output analysis. It is worth noting that with the PETIS detector system, areas of interest do not need to be defined until after data collection. This means that unexpected behaviour of a plant organ will be seen, which is not necessarily the case with conventional detector systems looking at predefined areas of interest.
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Input–output analysis of in vivo Photoassimilate translocation using Positron-Emitting Tracer Imaging System (PETIS) data
Journal of experimental botany, 2005Co-Authors: Anna J. Keutgen, Norbert Keutgen, Shinpei Matsuhashi, Chizuko Mizuniwa, Takehito Ito, Takashi Fujimura, N. S. Ishioka, Satoshi Watanabe, Akihiko Osa, Toshiaki SekineAbstract:The Positron-Emitting Tracer Imaging System (PETIS) is introduced for monitoring the distribution of C-11-labelled Photoassimilates in Sorghum. The obtained two-dimensional image data were quantitatively analysed using a transfer function analysis approach. While one half of a Sorghum root in a split root system was treated with either 0, 100, or 500 mM NaCl dissolved in the nutrient solution, tracer images of the root halves and the lower stem section were recorded using PETIS. From the observed tracer levels, parameters were estimated, from which the mean speed of tracer transport and the proportion of tracer moved between specified image positions were deduced. Transport speed varied between 0.7 and 1.8 cm min(-1) with the difference depending on which part of the stem was involved. When data were collected in the lowest 0.5-1 cm of the stem, which included the point where the roots emerge, transport speed was less. Rapid changes in NaCl concentration, from 0 to 100 mM, resulted in short-term increases of assimilate import into the treated root. This response represented a transient osmotic effect, that was compensated for in the medium-term by osmotic adaptation. Higher concentrations of NaCl (500 mM) resulted in distinctly less Photoassimilate transport into the treated root half. The present results agree with earlier observations, showing that transport of C-11-labelled Photoassimilates measured with the PETIS detector system can be quantified using the method of input-output analysis. It is worth noting that with the PETIS detector system, areas of interest do not need to be defined until after data collection. This means that unexpected behaviour of a plant organ will be seen, which is not necessarily the case with conventional detector systems looking at predefined areas of interest.
Satoshi Watanabe - One of the best experts on this subject based on the ideXlab platform.
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input output analysis of in vivo Photoassimilate translocation using positron emitting tracer imaging system petis data
Journal of Experimental Botany, 2005Co-Authors: Anna J. Keutgen, Norbert Keutgen, Shinpei Matsuhashi, Chizuko Mizuniwa, Takehito Ito, Takashi Fujimura, N. S. Ishioka, Satoshi Watanabe, Akihiko Osa, Toshiaki SekineAbstract:The Positron-Emitting Tracer Imaging System (PETIS) is introduced for monitoring the distribution of C-11-labelled Photoassimilates in Sorghum. The obtained two-dimensional image data were quantitatively analysed using a transfer function analysis approach. While one half of a Sorghum root in a split root system was treated with either 0, 100, or 500 mM NaCl dissolved in the nutrient solution, tracer images of the root halves and the lower stem section were recorded using PETIS. From the observed tracer levels, parameters were estimated, from which the mean speed of tracer transport and the proportion of tracer moved between specified image positions were deduced. Transport speed varied between 0.7 and 1.8 cm min(-1) with the difference depending on which part of the stem was involved. When data were collected in the lowest 0.5-1 cm of the stem, which included the point where the roots emerge, transport speed was less. Rapid changes in NaCl concentration, from 0 to 100 mM, resulted in short-term increases of assimilate import into the treated root. This response represented a transient osmotic effect, that was compensated for in the medium-term by osmotic adaptation. Higher concentrations of NaCl (500 mM) resulted in distinctly less Photoassimilate transport into the treated root half. The present results agree with earlier observations, showing that transport of C-11-labelled Photoassimilates measured with the PETIS detector system can be quantified using the method of input-output analysis. It is worth noting that with the PETIS detector system, areas of interest do not need to be defined until after data collection. This means that unexpected behaviour of a plant organ will be seen, which is not necessarily the case with conventional detector systems looking at predefined areas of interest.
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Input–output analysis of in vivo Photoassimilate translocation using Positron-Emitting Tracer Imaging System (PETIS) data
Journal of experimental botany, 2005Co-Authors: Anna J. Keutgen, Norbert Keutgen, Shinpei Matsuhashi, Chizuko Mizuniwa, Takehito Ito, Takashi Fujimura, N. S. Ishioka, Satoshi Watanabe, Akihiko Osa, Toshiaki SekineAbstract:The Positron-Emitting Tracer Imaging System (PETIS) is introduced for monitoring the distribution of C-11-labelled Photoassimilates in Sorghum. The obtained two-dimensional image data were quantitatively analysed using a transfer function analysis approach. While one half of a Sorghum root in a split root system was treated with either 0, 100, or 500 mM NaCl dissolved in the nutrient solution, tracer images of the root halves and the lower stem section were recorded using PETIS. From the observed tracer levels, parameters were estimated, from which the mean speed of tracer transport and the proportion of tracer moved between specified image positions were deduced. Transport speed varied between 0.7 and 1.8 cm min(-1) with the difference depending on which part of the stem was involved. When data were collected in the lowest 0.5-1 cm of the stem, which included the point where the roots emerge, transport speed was less. Rapid changes in NaCl concentration, from 0 to 100 mM, resulted in short-term increases of assimilate import into the treated root. This response represented a transient osmotic effect, that was compensated for in the medium-term by osmotic adaptation. Higher concentrations of NaCl (500 mM) resulted in distinctly less Photoassimilate transport into the treated root half. The present results agree with earlier observations, showing that transport of C-11-labelled Photoassimilates measured with the PETIS detector system can be quantified using the method of input-output analysis. It is worth noting that with the PETIS detector system, areas of interest do not need to be defined until after data collection. This means that unexpected behaviour of a plant organ will be seen, which is not necessarily the case with conventional detector systems looking at predefined areas of interest.
