The Experts below are selected from a list of 213711 Experts worldwide ranked by ideXlab platform

Haoshen Zhou - One of the best experts on this subject based on the ideXlab platform.

  • simultaneous voltammetric detection of dopamine and uric acid at their Physiological Level in the presence of ascorbic acid using poly acrylic acid multiwalled carbon nanotube composite covered glassy carbon electrode
    Biosensors and Bioelectronics, 2007
    Co-Authors: Aihua Liu, Itaru Honma, Haoshen Zhou
    Abstract:

    The use of poly(acrylic acid) (PAA)-multiwalled carbon-nanotubes (MWNTs) composite-coated glassy-carbon disk electrode (GCE) (PAA-MWNTs/GCE) for the simultaneous determination of Physiological Level dopamine (DA) and uric acid (UA) in the presence of an excess of ascorbic acid (AA) in a pH 7.4 phosphate-buffered solution was proposed. PAA-MWNTs composite was prepared by mixing of MWNTs powder into 1 mg/ml PAA aqueous solution under sonication. GCE surface was modified with PAA-MWNTs film by casting. AA demonstrates no voltammetric peak at PAA-MWNTs/GCE. The PAA-MWNTs composite is of a high surface area and of affinity for DA and UA adsorption. DA exhibits greatly improved electron-transfer rate and is electro-catalyzed at PAA-MWNTs/GCE. Moreover, the electro-catalytic oxidation of UA at PAA-MWNTs/GCE is observed, which makes it possible to detect lower Level UA. Therefore, the enhanced electrocatalytic currents for DA and UA were observed. The anodic peak currents at approximately 0.18 V and 0.35 V increase with the increasing concentrations of DA and UA, respectively, which correspond to the voltammetric peaks of DA and UA, respectively. The linear ranges are 40 nM to 3 microM DA and 0.3 microM to 10 microM UA in the presence of 0.3 mM AA. The lowest detection limits (S/N=3) were 20 nM DA and 110 nM UA.

  • simultaneous voltammetric detection of dopamine and uric acid at their Physiological Level in the presence of ascorbic acid using poly acrylic acid multiwalled carbon nanotube composite covered glassy carbon electrode
    Biosensors and Bioelectronics, 2007
    Co-Authors: Aihua Liu, Itaru Honma, Haoshen Zhou
    Abstract:

    Abstract The use of poly(acrylic acid) (PAA)-multiwalled carbon-nanotubes (MWNTs) composite-coated glassy-carbon disk electrode (GCE) (PAA-MWNTs/GCE) for the simultaneous determination of Physiological Level dopamine (DA) and uric acid (UA) in the presence of an excess of ascorbic acid (AA) in a pH 7.4 phosphate-buffered solution was proposed. PAA-MWNTs composite was prepared by mixing of MWNTs powder into 1 mg/ml PAA aqueous solution under sonication. GCE surface was modified with PAA-MWNTs film by casting. AA demonstrates no voltammetric peak at PAA-MWNTs/GCE. The PAA-MWNTs composite is of a high surface area and of affinity for DA and UA adsorption. DA exhibits greatly improved electron-transfer rate and is electro-catalyzed at PAA-MWNTs/GCE. Moreover, the electro-catalytic oxidation of UA at PAA-MWNTs/GCE is observed, which makes it possible to detect lower Level UA. Therefore, the enhanced electrocatalytic currents for DA and UA were observed. The anodic peak currents at ∼0.18 V and 0.35 V increase with the increasing concentrations of DA and UA, respectively, which correspond to the voltammetric peaks of DA and UA, respectively. The linear ranges are 40 nM to 3 μM DA and 0.3 μM to 10 μM UA in the presence of 0.3 mM AA. The lowest detection limits (S/N = 3) were 20 nM DA and 110 nM UA.

  • Electrochemical biosensor based on protein-polysaccharide hybrid for selective detection of nanomolar dopamine metabolite of 3,4-dihydroxyphenylacetic acid (DOPAC)
    Electrochemistry Communications, 2005
    Co-Authors: Aihua Liu, Itaru Honma, Haoshen Zhou
    Abstract:

    Abstract A strategy for the preparation of tyrosinase biosensor has been proposed to determine dopamine metabolite of 3,4-dihydroxyphenylacetic acid (DOPAC). An amino-polysaccharide chitosan was successfully used as matrix to entrap tyrosinase on glassy carbon electrode surface on basis of tyrosinase conjugated with chitosan. The sensor was operated at −0.15 V. The current linearly increased with the increasing concentration of DOPAC over the concentration of 6 nM to 0.2 mM. The sensor can be used to selectively determine nanomolar DOPAC in the presence of Physiological Level of ascorbic acid (AA), uric acid (UA), acetaminophen, neurotransmitters including dopamine, l -dopa, adrenaline and noradrenaline.

Sheila Ons - One of the best experts on this subject based on the ideXlab platform.

  • Nezara viridula (Hemiptera: Pentatomidae) transcriptomic analysis and neuropeptidomics
    Scientific Reports, 2018
    Co-Authors: Andrés Lavore, Lucila Perez-gianmarco, Natalia Esponda-behrens, Victorio Palacio, Maria Ines Catalano, Rolando Rivera-pomar, Sheila Ons
    Abstract:

    Stinkbugs (Hemiptera: Pentatomidae) are of major economic importance as pest of crops. Among the species composing the stinkbug complex, Nezara viridula is one of the most abundant in Brazil, Argentina and the Southern USA. However, this species has been poorly characterized at the genetic and Physiological Level. Here we sequenced and analyzed the complete transcriptome of N. viridula male and female adults. We identified neuropeptide precursor genes and G-protein coupled receptors for neuropeptides in this transcriptome. Mature neuropeptides were identified in N. viridula brain extracts by liquid chromatography-tandem mass spectrometry. We also analyzed the neuropeptide precursor complement in the genome sequence of Halyomorpha halys , another pentatomid of economic relevance. We compared the results in both pentatomids with the well-characterized neuropeptide repertoire from the kissing bug Rhodnius prolixus (Hemiptera: Reduviidae). We identified both group-specific features (which could be related to the different feeding habits) and similarities that could be characteristic of Heteroptera. This work contributes to a deeper knowledge of the genetic information of these pests, with a focus on neuroendocrine system characterization.

Aihua Liu - One of the best experts on this subject based on the ideXlab platform.

  • simultaneous voltammetric detection of dopamine and uric acid at their Physiological Level in the presence of ascorbic acid using poly acrylic acid multiwalled carbon nanotube composite covered glassy carbon electrode
    Biosensors and Bioelectronics, 2007
    Co-Authors: Aihua Liu, Itaru Honma, Haoshen Zhou
    Abstract:

    The use of poly(acrylic acid) (PAA)-multiwalled carbon-nanotubes (MWNTs) composite-coated glassy-carbon disk electrode (GCE) (PAA-MWNTs/GCE) for the simultaneous determination of Physiological Level dopamine (DA) and uric acid (UA) in the presence of an excess of ascorbic acid (AA) in a pH 7.4 phosphate-buffered solution was proposed. PAA-MWNTs composite was prepared by mixing of MWNTs powder into 1 mg/ml PAA aqueous solution under sonication. GCE surface was modified with PAA-MWNTs film by casting. AA demonstrates no voltammetric peak at PAA-MWNTs/GCE. The PAA-MWNTs composite is of a high surface area and of affinity for DA and UA adsorption. DA exhibits greatly improved electron-transfer rate and is electro-catalyzed at PAA-MWNTs/GCE. Moreover, the electro-catalytic oxidation of UA at PAA-MWNTs/GCE is observed, which makes it possible to detect lower Level UA. Therefore, the enhanced electrocatalytic currents for DA and UA were observed. The anodic peak currents at approximately 0.18 V and 0.35 V increase with the increasing concentrations of DA and UA, respectively, which correspond to the voltammetric peaks of DA and UA, respectively. The linear ranges are 40 nM to 3 microM DA and 0.3 microM to 10 microM UA in the presence of 0.3 mM AA. The lowest detection limits (S/N=3) were 20 nM DA and 110 nM UA.

  • simultaneous voltammetric detection of dopamine and uric acid at their Physiological Level in the presence of ascorbic acid using poly acrylic acid multiwalled carbon nanotube composite covered glassy carbon electrode
    Biosensors and Bioelectronics, 2007
    Co-Authors: Aihua Liu, Itaru Honma, Haoshen Zhou
    Abstract:

    Abstract The use of poly(acrylic acid) (PAA)-multiwalled carbon-nanotubes (MWNTs) composite-coated glassy-carbon disk electrode (GCE) (PAA-MWNTs/GCE) for the simultaneous determination of Physiological Level dopamine (DA) and uric acid (UA) in the presence of an excess of ascorbic acid (AA) in a pH 7.4 phosphate-buffered solution was proposed. PAA-MWNTs composite was prepared by mixing of MWNTs powder into 1 mg/ml PAA aqueous solution under sonication. GCE surface was modified with PAA-MWNTs film by casting. AA demonstrates no voltammetric peak at PAA-MWNTs/GCE. The PAA-MWNTs composite is of a high surface area and of affinity for DA and UA adsorption. DA exhibits greatly improved electron-transfer rate and is electro-catalyzed at PAA-MWNTs/GCE. Moreover, the electro-catalytic oxidation of UA at PAA-MWNTs/GCE is observed, which makes it possible to detect lower Level UA. Therefore, the enhanced electrocatalytic currents for DA and UA were observed. The anodic peak currents at ∼0.18 V and 0.35 V increase with the increasing concentrations of DA and UA, respectively, which correspond to the voltammetric peaks of DA and UA, respectively. The linear ranges are 40 nM to 3 μM DA and 0.3 μM to 10 μM UA in the presence of 0.3 mM AA. The lowest detection limits (S/N = 3) were 20 nM DA and 110 nM UA.

  • Electrochemical biosensor based on protein-polysaccharide hybrid for selective detection of nanomolar dopamine metabolite of 3,4-dihydroxyphenylacetic acid (DOPAC)
    Electrochemistry Communications, 2005
    Co-Authors: Aihua Liu, Itaru Honma, Haoshen Zhou
    Abstract:

    Abstract A strategy for the preparation of tyrosinase biosensor has been proposed to determine dopamine metabolite of 3,4-dihydroxyphenylacetic acid (DOPAC). An amino-polysaccharide chitosan was successfully used as matrix to entrap tyrosinase on glassy carbon electrode surface on basis of tyrosinase conjugated with chitosan. The sensor was operated at −0.15 V. The current linearly increased with the increasing concentration of DOPAC over the concentration of 6 nM to 0.2 mM. The sensor can be used to selectively determine nanomolar DOPAC in the presence of Physiological Level of ascorbic acid (AA), uric acid (UA), acetaminophen, neurotransmitters including dopamine, l -dopa, adrenaline and noradrenaline.

Andrés Lavore - One of the best experts on this subject based on the ideXlab platform.

  • Nezara viridula (Hemiptera: Pentatomidae) transcriptomic analysis and neuropeptidomics
    Scientific Reports, 2018
    Co-Authors: Andrés Lavore, Lucila Perez-gianmarco, Natalia Esponda-behrens, Victorio Palacio, Maria Ines Catalano, Rolando Rivera-pomar, Sheila Ons
    Abstract:

    Stinkbugs (Hemiptera: Pentatomidae) are of major economic importance as pest of crops. Among the species composing the stinkbug complex, Nezara viridula is one of the most abundant in Brazil, Argentina and the Southern USA. However, this species has been poorly characterized at the genetic and Physiological Level. Here we sequenced and analyzed the complete transcriptome of N. viridula male and female adults. We identified neuropeptide precursor genes and G-protein coupled receptors for neuropeptides in this transcriptome. Mature neuropeptides were identified in N. viridula brain extracts by liquid chromatography-tandem mass spectrometry. We also analyzed the neuropeptide precursor complement in the genome sequence of Halyomorpha halys , another pentatomid of economic relevance. We compared the results in both pentatomids with the well-characterized neuropeptide repertoire from the kissing bug Rhodnius prolixus (Hemiptera: Reduviidae). We identified both group-specific features (which could be related to the different feeding habits) and similarities that could be characteristic of Heteroptera. This work contributes to a deeper knowledge of the genetic information of these pests, with a focus on neuroendocrine system characterization.

Itaru Honma - One of the best experts on this subject based on the ideXlab platform.

  • simultaneous voltammetric detection of dopamine and uric acid at their Physiological Level in the presence of ascorbic acid using poly acrylic acid multiwalled carbon nanotube composite covered glassy carbon electrode
    Biosensors and Bioelectronics, 2007
    Co-Authors: Aihua Liu, Itaru Honma, Haoshen Zhou
    Abstract:

    The use of poly(acrylic acid) (PAA)-multiwalled carbon-nanotubes (MWNTs) composite-coated glassy-carbon disk electrode (GCE) (PAA-MWNTs/GCE) for the simultaneous determination of Physiological Level dopamine (DA) and uric acid (UA) in the presence of an excess of ascorbic acid (AA) in a pH 7.4 phosphate-buffered solution was proposed. PAA-MWNTs composite was prepared by mixing of MWNTs powder into 1 mg/ml PAA aqueous solution under sonication. GCE surface was modified with PAA-MWNTs film by casting. AA demonstrates no voltammetric peak at PAA-MWNTs/GCE. The PAA-MWNTs composite is of a high surface area and of affinity for DA and UA adsorption. DA exhibits greatly improved electron-transfer rate and is electro-catalyzed at PAA-MWNTs/GCE. Moreover, the electro-catalytic oxidation of UA at PAA-MWNTs/GCE is observed, which makes it possible to detect lower Level UA. Therefore, the enhanced electrocatalytic currents for DA and UA were observed. The anodic peak currents at approximately 0.18 V and 0.35 V increase with the increasing concentrations of DA and UA, respectively, which correspond to the voltammetric peaks of DA and UA, respectively. The linear ranges are 40 nM to 3 microM DA and 0.3 microM to 10 microM UA in the presence of 0.3 mM AA. The lowest detection limits (S/N=3) were 20 nM DA and 110 nM UA.

  • simultaneous voltammetric detection of dopamine and uric acid at their Physiological Level in the presence of ascorbic acid using poly acrylic acid multiwalled carbon nanotube composite covered glassy carbon electrode
    Biosensors and Bioelectronics, 2007
    Co-Authors: Aihua Liu, Itaru Honma, Haoshen Zhou
    Abstract:

    Abstract The use of poly(acrylic acid) (PAA)-multiwalled carbon-nanotubes (MWNTs) composite-coated glassy-carbon disk electrode (GCE) (PAA-MWNTs/GCE) for the simultaneous determination of Physiological Level dopamine (DA) and uric acid (UA) in the presence of an excess of ascorbic acid (AA) in a pH 7.4 phosphate-buffered solution was proposed. PAA-MWNTs composite was prepared by mixing of MWNTs powder into 1 mg/ml PAA aqueous solution under sonication. GCE surface was modified with PAA-MWNTs film by casting. AA demonstrates no voltammetric peak at PAA-MWNTs/GCE. The PAA-MWNTs composite is of a high surface area and of affinity for DA and UA adsorption. DA exhibits greatly improved electron-transfer rate and is electro-catalyzed at PAA-MWNTs/GCE. Moreover, the electro-catalytic oxidation of UA at PAA-MWNTs/GCE is observed, which makes it possible to detect lower Level UA. Therefore, the enhanced electrocatalytic currents for DA and UA were observed. The anodic peak currents at ∼0.18 V and 0.35 V increase with the increasing concentrations of DA and UA, respectively, which correspond to the voltammetric peaks of DA and UA, respectively. The linear ranges are 40 nM to 3 μM DA and 0.3 μM to 10 μM UA in the presence of 0.3 mM AA. The lowest detection limits (S/N = 3) were 20 nM DA and 110 nM UA.

  • Electrochemical biosensor based on protein-polysaccharide hybrid for selective detection of nanomolar dopamine metabolite of 3,4-dihydroxyphenylacetic acid (DOPAC)
    Electrochemistry Communications, 2005
    Co-Authors: Aihua Liu, Itaru Honma, Haoshen Zhou
    Abstract:

    Abstract A strategy for the preparation of tyrosinase biosensor has been proposed to determine dopamine metabolite of 3,4-dihydroxyphenylacetic acid (DOPAC). An amino-polysaccharide chitosan was successfully used as matrix to entrap tyrosinase on glassy carbon electrode surface on basis of tyrosinase conjugated with chitosan. The sensor was operated at −0.15 V. The current linearly increased with the increasing concentration of DOPAC over the concentration of 6 nM to 0.2 mM. The sensor can be used to selectively determine nanomolar DOPAC in the presence of Physiological Level of ascorbic acid (AA), uric acid (UA), acetaminophen, neurotransmitters including dopamine, l -dopa, adrenaline and noradrenaline.