The Experts below are selected from a list of 13764 Experts worldwide ranked by ideXlab platform
Nicholas P Harberd - One of the best experts on this subject based on the ideXlab platform.
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ethylene regulates arabidopsis development via the modulation of della protein growth repressor function
The Plant Cell, 2003Co-Authors: Patrick Achard, Dominique Van Der Straeten, Willem Vriezen, Nicholas P HarberdAbstract:Phytohormones regulate plant development via a poorly understood signal response network. Here, we show that the Phytohormone ethylene regulates plant development at least in part via alteration of the properties of DELLA protein nuclear growth repressors, a family of proteins first identified as gibberellin (GA) signaling components. This conclusion is based on the following experimental observations. First, ethylene inhibited Arabidopsis root growth in a DELLA-dependent manner. Second, ethylene delayed the GA-induced disappearance of the DELLA protein repressor of ga1-3 from root cell nuclei via a constitutive triple response-dependent signaling pathway. Third, the ethylene-promoted "apical hook" structure of etiolated seedling hypocotyls was dependent on the relief of DELLA-mediated growth restraint. Ethylene, auxin, and GA responses now can be attributed to effects on DELLA function, suggesting that DELLA plays a key integrative role in the Phytohormone signal response network.
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ethylene regulates arabidopsis development via the modulation of della protein growth repressor function
The Plant Cell, 2003Co-Authors: Patrick Achard, Willem Vriezen, Dominique Van Der Straeten, Nicholas P HarberdAbstract:Phytohormones regulate plant development via a poorly understood signal response network. Here, we show that the Phytohormone ethylene regulates plant development at least in part via alteration of the properties of DELLA protein nuclear growth repressors, a family of proteins first identified as gibberellin (GA) signaling components. This conclusion is based on the following experimental observations. First, ethylene inhibited Arabidopsis root growth in a DELLA-dependent manner. Second, ethylene delayed the GA-induced disappearance of the DELLA protein repressor of ga1-3 from root cell nuclei via a constitutive triple response-dependent signaling pathway. Third, the ethylene-promoted “apical hook” structure of etiolated seedling hypocotyls was dependent on the relief of DELLA-mediated growth restraint. Ethylene, auxin, and GA responses now can be attributed to effects on DELLA function, suggesting that DELLA plays a key integrative role in the Phytohormone signal response network.
Patrick Achard - One of the best experts on this subject based on the ideXlab platform.
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ethylene regulates arabidopsis development via the modulation of della protein growth repressor function
The Plant Cell, 2003Co-Authors: Patrick Achard, Dominique Van Der Straeten, Willem Vriezen, Nicholas P HarberdAbstract:Phytohormones regulate plant development via a poorly understood signal response network. Here, we show that the Phytohormone ethylene regulates plant development at least in part via alteration of the properties of DELLA protein nuclear growth repressors, a family of proteins first identified as gibberellin (GA) signaling components. This conclusion is based on the following experimental observations. First, ethylene inhibited Arabidopsis root growth in a DELLA-dependent manner. Second, ethylene delayed the GA-induced disappearance of the DELLA protein repressor of ga1-3 from root cell nuclei via a constitutive triple response-dependent signaling pathway. Third, the ethylene-promoted "apical hook" structure of etiolated seedling hypocotyls was dependent on the relief of DELLA-mediated growth restraint. Ethylene, auxin, and GA responses now can be attributed to effects on DELLA function, suggesting that DELLA plays a key integrative role in the Phytohormone signal response network.
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ethylene regulates arabidopsis development via the modulation of della protein growth repressor function
The Plant Cell, 2003Co-Authors: Patrick Achard, Willem Vriezen, Dominique Van Der Straeten, Nicholas P HarberdAbstract:Phytohormones regulate plant development via a poorly understood signal response network. Here, we show that the Phytohormone ethylene regulates plant development at least in part via alteration of the properties of DELLA protein nuclear growth repressors, a family of proteins first identified as gibberellin (GA) signaling components. This conclusion is based on the following experimental observations. First, ethylene inhibited Arabidopsis root growth in a DELLA-dependent manner. Second, ethylene delayed the GA-induced disappearance of the DELLA protein repressor of ga1-3 from root cell nuclei via a constitutive triple response-dependent signaling pathway. Third, the ethylene-promoted “apical hook” structure of etiolated seedling hypocotyls was dependent on the relief of DELLA-mediated growth restraint. Ethylene, auxin, and GA responses now can be attributed to effects on DELLA function, suggesting that DELLA plays a key integrative role in the Phytohormone signal response network.
Willem Vriezen - One of the best experts on this subject based on the ideXlab platform.
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ethylene regulates arabidopsis development via the modulation of della protein growth repressor function
The Plant Cell, 2003Co-Authors: Patrick Achard, Dominique Van Der Straeten, Willem Vriezen, Nicholas P HarberdAbstract:Phytohormones regulate plant development via a poorly understood signal response network. Here, we show that the Phytohormone ethylene regulates plant development at least in part via alteration of the properties of DELLA protein nuclear growth repressors, a family of proteins first identified as gibberellin (GA) signaling components. This conclusion is based on the following experimental observations. First, ethylene inhibited Arabidopsis root growth in a DELLA-dependent manner. Second, ethylene delayed the GA-induced disappearance of the DELLA protein repressor of ga1-3 from root cell nuclei via a constitutive triple response-dependent signaling pathway. Third, the ethylene-promoted "apical hook" structure of etiolated seedling hypocotyls was dependent on the relief of DELLA-mediated growth restraint. Ethylene, auxin, and GA responses now can be attributed to effects on DELLA function, suggesting that DELLA plays a key integrative role in the Phytohormone signal response network.
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ethylene regulates arabidopsis development via the modulation of della protein growth repressor function
The Plant Cell, 2003Co-Authors: Patrick Achard, Willem Vriezen, Dominique Van Der Straeten, Nicholas P HarberdAbstract:Phytohormones regulate plant development via a poorly understood signal response network. Here, we show that the Phytohormone ethylene regulates plant development at least in part via alteration of the properties of DELLA protein nuclear growth repressors, a family of proteins first identified as gibberellin (GA) signaling components. This conclusion is based on the following experimental observations. First, ethylene inhibited Arabidopsis root growth in a DELLA-dependent manner. Second, ethylene delayed the GA-induced disappearance of the DELLA protein repressor of ga1-3 from root cell nuclei via a constitutive triple response-dependent signaling pathway. Third, the ethylene-promoted “apical hook” structure of etiolated seedling hypocotyls was dependent on the relief of DELLA-mediated growth restraint. Ethylene, auxin, and GA responses now can be attributed to effects on DELLA function, suggesting that DELLA plays a key integrative role in the Phytohormone signal response network.
Hong Zhang - One of the best experts on this subject based on the ideXlab platform.
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analysis of Phytohormones in vermicompost using a novel combinative sample preparation strategy of ultrasound assisted extraction and solid phase extraction coupled with liquid chromatography tandem mass spectrometry
Talanta, 2015Co-Authors: Hong Zhang, Swee Ngin Tan, Chee How Teo, Yan Ru Yew, Xin Chen, Jean Wan Hong YongAbstract:Abstract Vermicompost (VC), a widely used premium organic fertilizer, is the by-product of symbiotic interactions between earthworms and microorganisms living within them. It has been postulated that Phytohormones are plausible “magic compounds” in VC that are responsible for making them such good fertilizers. Thus, a novel approach involving ultrasound-assisted extraction (UAE) and solid-phase extraction (SPE) was developed as a fast and efficient sample preparation method to screen for different classes of Phytohormones in VC by liquid chromatography–tandem mass spectrometric (LC–MS/MS) analysis. Nine Phytohormones from three different classes, including trans-zeatin (tZ), kinetin (K), N6-[2-isopentyl]adenine (iP), N6-benzyladenine (BA), N6-isopentenyladenosine (iPR), indole-3-acetic acid (IAA), 4-[3-indolyl]butyric acid (IBA), 1-naphthaleneacetic acid (NAA) and (+)-abscisic acid (ABA), were simultaneously screened. The extraction parameters influencing UAE efficiency were optimized to provide comparable recovery to the conventional mix-stirring (MSt) method. The optimized UAE method was subsequently applied on the analysis of Phytohormones in VC, i.e. Phytohormone extract was further pre-concentrated and purified using C18 and MCX SPE cartridges prior to LC–MS/MS analysis. The following Phytohormones, namely iP, iPR and IAA, were detected and quantified to be 0.49, 0.53, 79.78 ng g−1, respectively; tZ was found to be below the limit of quantitation. Recoveries of 10.2%, 9.1%, 18.9% and 0.3% for tZ, iP, iPR and IAA were obtained. This is one of the few reported works for the successful detection and quantitation of cytokinins and auxins in VC, that provided the key empirical evidence to explain the growth efficacy of applying VC in promoting plant growth. Additionally, this pioneering work could potentially be applicable for the analysis of other types of organic fertilizers such as composts and activated composted materials awaiting Phytohormone analyzes for quality assessment and control.
Jean Wan Hong Yong - One of the best experts on this subject based on the ideXlab platform.
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analysis of Phytohormones in vermicompost using a novel combinative sample preparation strategy of ultrasound assisted extraction and solid phase extraction coupled with liquid chromatography tandem mass spectrometry
Talanta, 2015Co-Authors: Hong Zhang, Swee Ngin Tan, Chee How Teo, Yan Ru Yew, Xin Chen, Jean Wan Hong YongAbstract:Abstract Vermicompost (VC), a widely used premium organic fertilizer, is the by-product of symbiotic interactions between earthworms and microorganisms living within them. It has been postulated that Phytohormones are plausible “magic compounds” in VC that are responsible for making them such good fertilizers. Thus, a novel approach involving ultrasound-assisted extraction (UAE) and solid-phase extraction (SPE) was developed as a fast and efficient sample preparation method to screen for different classes of Phytohormones in VC by liquid chromatography–tandem mass spectrometric (LC–MS/MS) analysis. Nine Phytohormones from three different classes, including trans-zeatin (tZ), kinetin (K), N6-[2-isopentyl]adenine (iP), N6-benzyladenine (BA), N6-isopentenyladenosine (iPR), indole-3-acetic acid (IAA), 4-[3-indolyl]butyric acid (IBA), 1-naphthaleneacetic acid (NAA) and (+)-abscisic acid (ABA), were simultaneously screened. The extraction parameters influencing UAE efficiency were optimized to provide comparable recovery to the conventional mix-stirring (MSt) method. The optimized UAE method was subsequently applied on the analysis of Phytohormones in VC, i.e. Phytohormone extract was further pre-concentrated and purified using C18 and MCX SPE cartridges prior to LC–MS/MS analysis. The following Phytohormones, namely iP, iPR and IAA, were detected and quantified to be 0.49, 0.53, 79.78 ng g−1, respectively; tZ was found to be below the limit of quantitation. Recoveries of 10.2%, 9.1%, 18.9% and 0.3% for tZ, iP, iPR and IAA were obtained. This is one of the few reported works for the successful detection and quantitation of cytokinins and auxins in VC, that provided the key empirical evidence to explain the growth efficacy of applying VC in promoting plant growth. Additionally, this pioneering work could potentially be applicable for the analysis of other types of organic fertilizers such as composts and activated composted materials awaiting Phytohormone analyzes for quality assessment and control.