The Experts below are selected from a list of 93 Experts worldwide ranked by ideXlab platform
Ye Sun Han - One of the best experts on this subject based on the ideXlab platform.
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expression of the active human and duck hepatitis b virus polymerases in heterologous system of Pichia Methanolica
Antiviral Research, 2002Co-Authors: Juhyun Choi, Eunice E Kim, Young In Park, Ye Sun HanAbstract:We expressed the Hepatitis B virus polymerase (HBV P protein) using a recently introduced yeast system, Pichia Methanolica. HBV (1-680 amino acids) and Duck Hepatitis B virus (DHBV, 1-780 amino acids) polymerase were expressed and showed DNA dependent DNA polymerase (DDDP). The DHBV polymerase had RNA dependent DNA polymerase (RDDP) and RNase H activities. We present a new simplified way of obtaining active viral P protein using the yeast expression system. The viral P proteins proved to be stable and were not aggregated in the yeast system.
Baggio, Marcia S. - One of the best experts on this subject based on the ideXlab platform.
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Methionine-induced hyperhomocysteinemia reverts fibrinolytic pathway activation in a murine model of acute promyelocytic leukemia
AMER SOC HEMATOLOGY, 2012Co-Authors: Jacomo, Rafael H., Santana-lemos, Barbara A., Lima, Ana Silvia G., Assis, Patricia A., Lange, Ana Paula A., Figueiredo-pontes, Lorena L., Oliveira, Luciana O., Bassi, Sarah C., Benicio, Mariana T. L., Baggio, Marcia S.Abstract:Increased fibrinolysis is an important component of acute promyelocytic leukemia (APL) bleeding diathesis. APL blasts overexpress annexin II (ANXII), a receptor for tissue plasminogen activator (tPA), and plasminogen, thereby increasing plasmin generation. Previous studies suggested that ANXII plays a pivotal role in APL coagulopathy. ANXII binding to tPA can be inhibited by homocysteine and hyperhomocysteinemia can be induced by L-methionine supplementation. In the present study, we used an APL mouse model to study ANXII function and the effects of hyperhomocysteinemia in vivo. Leukemic cells expressed higher ANXII and tPA plasma levels (11.95 ng/mL in leukemic vs 10.74 ng/mL in wild-type; P = .004). In leukemic mice, administration of L-methionine significantly increased homocysteine levels (49.0 mu mol/mL and < 6.0 mu mol/mL in the treated and nontreated groups, respectively) and reduced tPA levels to baseline concentrations. The latter were also decreased after infusion of the LCKLSL peptide, a competitor for the ANXII tPA-binding site (11.07 ng/mL; P = .001). We also expressed and purified the p36 component of ANXII in Pichia Methanolica. The infusion of p36 in wild-type mice increased tPA and thrombin-antithrombin levels, and the latter was reversed by L-methionine administration. The results of the present study demonstrate the relevance of ANXII in vivo and suggest that methionine-induced hyperhomocysteinemia may reverse hyperfibrinolysis in APL. (Blood. 2012;120(1):207-213
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Methionine-induced hyperhomocysteinemia reverts fibrinolytic pathway activation in a murine model of acute promyelocytic leukemia
WASHINGTON, 2012Co-Authors: Jacomo, Rafael H., Santana-lemos, Barbara A., Lima, Ana Silvia G., Assis, Patricia A., Lange, Ana Paula A., Figueiredo-pontes, Lorena L., Oliveira, Luciana O., Bassi, Sarah C., Benicio, Mariana T. L., Baggio, Marcia S.Abstract:Increased fibrinolysis is an important component of acute promyelocytic leukemia (APL) bleeding diathesis. APL blasts overexpress annexin II (ANXII), a receptor for tissue plasminogen activator (tPA), and plasminogen, thereby increasing plasmin generation. Previous studies suggested that ANXII plays a pivotal role in APL coagulopathy. ANXII binding to tPA can be inhibited by homocysteine and hyperhomocysteinemia can be induced by L-methionine supplementation. In the present study, we used an APL mouse model to study ANXII function and the effects of hyperhomocysteinemia in vivo. Leukemic cells expressed higher ANXII and tPA plasma levels (11.95 ng/mL in leukemic vs 10.74 ng/mL in wild-type; P = .004). In leukemic mice, administration of L-methionine significantly increased homocysteine levels (49.0 mu mol/mL and < 6.0 mu mol/mL in the treated and nontreated groups, respectively) and reduced tPA levels to baseline concentrations. The latter were also decreased after infusion of the LCKLSL peptide, a competitor for the ANXII tPA-binding site (11.07 ng/mL; P = .001). We also expressed and purified the p36 component of ANXII in Pichia Methanolica. The infusion of p36 in wild-type mice increased tPA and thrombin-antithrombin levels, and the latter was reversed by L-methionine administration. The results of the present study demonstrate the relevance of ANXII in vivo and suggest that methionine-induced hyperhomocysteinemia may reverse hyperfibrinolysis in APL. (Blood. 2012;120(1):207-213)Conselho Nacional de Desenvolvimento Cientifico e TecnologicoConselho Nacional de Desenvolvimento Cientifico e Tecnologico [573754/2008-0]Fundacao de Apoio a Pesquisa do Estado de Sao Paulo [1998/14247-6, 02/11086-9]Fundacao de Apoio a Pesquisa do Estado de Sao Paul
Juhyun Choi - One of the best experts on this subject based on the ideXlab platform.
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expression of the active human and duck hepatitis b virus polymerases in heterologous system of Pichia Methanolica
Antiviral Research, 2002Co-Authors: Juhyun Choi, Eunice E Kim, Young In Park, Ye Sun HanAbstract:We expressed the Hepatitis B virus polymerase (HBV P protein) using a recently introduced yeast system, Pichia Methanolica. HBV (1-680 amino acids) and Duck Hepatitis B virus (DHBV, 1-780 amino acids) polymerase were expressed and showed DNA dependent DNA polymerase (DDDP). The DHBV polymerase had RNA dependent DNA polymerase (RDDP) and RNase H activities. We present a new simplified way of obtaining active viral P protein using the yeast expression system. The viral P proteins proved to be stable and were not aggregated in the yeast system.
Jacomo, Rafael H. - One of the best experts on this subject based on the ideXlab platform.
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Methionine-induced hyperhomocysteinemia reverts fibrinolytic pathway activation in a murine model of acute promyelocytic leukemia
AMER SOC HEMATOLOGY, 2012Co-Authors: Jacomo, Rafael H., Santana-lemos, Barbara A., Lima, Ana Silvia G., Assis, Patricia A., Lange, Ana Paula A., Figueiredo-pontes, Lorena L., Oliveira, Luciana O., Bassi, Sarah C., Benicio, Mariana T. L., Baggio, Marcia S.Abstract:Increased fibrinolysis is an important component of acute promyelocytic leukemia (APL) bleeding diathesis. APL blasts overexpress annexin II (ANXII), a receptor for tissue plasminogen activator (tPA), and plasminogen, thereby increasing plasmin generation. Previous studies suggested that ANXII plays a pivotal role in APL coagulopathy. ANXII binding to tPA can be inhibited by homocysteine and hyperhomocysteinemia can be induced by L-methionine supplementation. In the present study, we used an APL mouse model to study ANXII function and the effects of hyperhomocysteinemia in vivo. Leukemic cells expressed higher ANXII and tPA plasma levels (11.95 ng/mL in leukemic vs 10.74 ng/mL in wild-type; P = .004). In leukemic mice, administration of L-methionine significantly increased homocysteine levels (49.0 mu mol/mL and < 6.0 mu mol/mL in the treated and nontreated groups, respectively) and reduced tPA levels to baseline concentrations. The latter were also decreased after infusion of the LCKLSL peptide, a competitor for the ANXII tPA-binding site (11.07 ng/mL; P = .001). We also expressed and purified the p36 component of ANXII in Pichia Methanolica. The infusion of p36 in wild-type mice increased tPA and thrombin-antithrombin levels, and the latter was reversed by L-methionine administration. The results of the present study demonstrate the relevance of ANXII in vivo and suggest that methionine-induced hyperhomocysteinemia may reverse hyperfibrinolysis in APL. (Blood. 2012;120(1):207-213
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Methionine-induced hyperhomocysteinemia reverts fibrinolytic pathway activation in a murine model of acute promyelocytic leukemia
WASHINGTON, 2012Co-Authors: Jacomo, Rafael H., Santana-lemos, Barbara A., Lima, Ana Silvia G., Assis, Patricia A., Lange, Ana Paula A., Figueiredo-pontes, Lorena L., Oliveira, Luciana O., Bassi, Sarah C., Benicio, Mariana T. L., Baggio, Marcia S.Abstract:Increased fibrinolysis is an important component of acute promyelocytic leukemia (APL) bleeding diathesis. APL blasts overexpress annexin II (ANXII), a receptor for tissue plasminogen activator (tPA), and plasminogen, thereby increasing plasmin generation. Previous studies suggested that ANXII plays a pivotal role in APL coagulopathy. ANXII binding to tPA can be inhibited by homocysteine and hyperhomocysteinemia can be induced by L-methionine supplementation. In the present study, we used an APL mouse model to study ANXII function and the effects of hyperhomocysteinemia in vivo. Leukemic cells expressed higher ANXII and tPA plasma levels (11.95 ng/mL in leukemic vs 10.74 ng/mL in wild-type; P = .004). In leukemic mice, administration of L-methionine significantly increased homocysteine levels (49.0 mu mol/mL and < 6.0 mu mol/mL in the treated and nontreated groups, respectively) and reduced tPA levels to baseline concentrations. The latter were also decreased after infusion of the LCKLSL peptide, a competitor for the ANXII tPA-binding site (11.07 ng/mL; P = .001). We also expressed and purified the p36 component of ANXII in Pichia Methanolica. The infusion of p36 in wild-type mice increased tPA and thrombin-antithrombin levels, and the latter was reversed by L-methionine administration. The results of the present study demonstrate the relevance of ANXII in vivo and suggest that methionine-induced hyperhomocysteinemia may reverse hyperfibrinolysis in APL. (Blood. 2012;120(1):207-213)Conselho Nacional de Desenvolvimento Cientifico e TecnologicoConselho Nacional de Desenvolvimento Cientifico e Tecnologico [573754/2008-0]Fundacao de Apoio a Pesquisa do Estado de Sao Paulo [1998/14247-6, 02/11086-9]Fundacao de Apoio a Pesquisa do Estado de Sao Paul
Young In Park - One of the best experts on this subject based on the ideXlab platform.
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expression of the active human and duck hepatitis b virus polymerases in heterologous system of Pichia Methanolica
Antiviral Research, 2002Co-Authors: Juhyun Choi, Eunice E Kim, Young In Park, Ye Sun HanAbstract:We expressed the Hepatitis B virus polymerase (HBV P protein) using a recently introduced yeast system, Pichia Methanolica. HBV (1-680 amino acids) and Duck Hepatitis B virus (DHBV, 1-780 amino acids) polymerase were expressed and showed DNA dependent DNA polymerase (DDDP). The DHBV polymerase had RNA dependent DNA polymerase (RDDP) and RNase H activities. We present a new simplified way of obtaining active viral P protein using the yeast expression system. The viral P proteins proved to be stable and were not aggregated in the yeast system.