The Experts below are selected from a list of 18 Experts worldwide ranked by ideXlab platform

Hartung J. S. - One of the best experts on this subject based on the ideXlab platform.

  • Citrus and coffee strains of Xylella fastidiosa induce Pierce's Disease in grapevine
    Amer Phytopathological Soc, 2002
    Co-Authors: Li W. B., Zhou C. H., Pria W. D., Teixeira D. C., Miranda V. S., Pereira E. O., Ayres A. J., Hartung J. S.
    Abstract:

    Xylella fastidiosa causes citrus variegated chlorosis (CVC) Disease in Brazil and Pierce's Disease of grapevines in the United States. Both of these Diseases cause significant production problems in the respective industries. The recent establishment of the glassy-winged sharpshooter in California has radically increased the threat posed by Pierces Disease to California viticulture. Populations of this insect reach very high levels in citrus groves in California and move from the orchards into the vineyards, where they acquire inoculum and spread Pierce's Disease in the vineyards. Here we show that strains of X. fastidiosa isolated from Diseased citrus and coffee in Brazil can incite symptoms of Pierce's Disease after mechanical inoculation into seven commercial Vitis vinifera varieties grown in Brazil and California. Thus, any future introduction of the CVC strains of X. fastidiosa into the United States would pose a threat to both the sweet orange and grapevine industries. Previous work has clearly shown that the strains of X. fastidiosa isolated from Pierce's Disease- and CVC-affected plants are the most distantly related of all strains in the diverse taxon X. fastidiosa. The ability of citrus strains of X. fastidiosa to incite Disease in grapevine is therefore surprising and creates an experimental system with which to dissect mechanisms used by X.,fastidiosa in plant colonization and Disease development using the full genome sequence data that has recently become available for both the citrus and grapevine strains of this pathogen

Li W. B. - One of the best experts on this subject based on the ideXlab platform.

  • Citrus and coffee strains of Xylella fastidiosa induce Pierce's Disease in grapevine
    Amer Phytopathological Soc, 2002
    Co-Authors: Li W. B., Zhou C. H., Pria W. D., Teixeira D. C., Miranda V. S., Pereira E. O., Ayres A. J., Hartung J. S.
    Abstract:

    Xylella fastidiosa causes citrus variegated chlorosis (CVC) Disease in Brazil and Pierce's Disease of grapevines in the United States. Both of these Diseases cause significant production problems in the respective industries. The recent establishment of the glassy-winged sharpshooter in California has radically increased the threat posed by Pierces Disease to California viticulture. Populations of this insect reach very high levels in citrus groves in California and move from the orchards into the vineyards, where they acquire inoculum and spread Pierce's Disease in the vineyards. Here we show that strains of X. fastidiosa isolated from Diseased citrus and coffee in Brazil can incite symptoms of Pierce's Disease after mechanical inoculation into seven commercial Vitis vinifera varieties grown in Brazil and California. Thus, any future introduction of the CVC strains of X. fastidiosa into the United States would pose a threat to both the sweet orange and grapevine industries. Previous work has clearly shown that the strains of X. fastidiosa isolated from Pierce's Disease- and CVC-affected plants are the most distantly related of all strains in the diverse taxon X. fastidiosa. The ability of citrus strains of X. fastidiosa to incite Disease in grapevine is therefore surprising and creates an experimental system with which to dissect mechanisms used by X.,fastidiosa in plant colonization and Disease development using the full genome sequence data that has recently become available for both the citrus and grapevine strains of this pathogen

Vest, Mandi Ann - One of the best experts on this subject based on the ideXlab platform.

  • Investigations on the diagnosis, colonization, and epidemiology of grapevines with Pierce's Disease
    Texas A&M University, 2005
    Co-Authors: Vest, Mandi Ann
    Abstract:

    Pierces Disease (PD) of grapevines, caused by Xylella fastidiosa, is devastating Texas vineyards. Two rapid diagnostic techniques, real-time polymerase chain reaction (PCR) and enzyme-linked immunosorbent assay (ELISA), were compared on the basis of cost, reliability, and their ability to quantify X. fastidiosa in Diseased tissues. A high correlation was found between the two techniques for measuring bacterial titer in vitro. A similar relationship was not detected when applying the methods to Diseased tissue. There was a 75% similarity between the techniques when used to diagnose PD in artificially infected grapevines. Where the two methods differed, real-time PCR was more successful in identifying plants known to be infected with the bacterium. In uninoculated grapevines, the two techniques were similar, where the positive rates were 7% and 4% for ELISA and real-time PCR respectively. In a second study, 3 grape cultivars, Cynthiana, Cabernet Sauvignon, and Chardonnay, were inoculated with 2 isolates of X. fastidiosa to measure Disease development and colonization by the pathogen. The bacteria colonized similar distances from the inoculation point over a 25 week period in all three cultivars. Real-time PCR and ELISA absorbance values suggest that the concentrations of bacteria ranged between 104 and 106 cells/ml in a 1.27 cm section of grapevine cane. Concentrations of bacteria didnt vary based on distance from the inoculation point. Marginal leaf-scorch symptoms were seen on Cabernet Sauvignon and Chardonnay grapevines 9 weeks post-inoculation. Leaf-scorch symptoms were not observed on Cynthiana. The vigor of all inoculated grapevines was reduced compared to negative control grapevines the season after initial infection. In a third study, a Texas vineyard planted in Viognier grapevines was surveyed for PD symptoms on 3 separate dates. In October 2003, 45/50 rows had significant aggregation of symptomatic grapevines according to Ordinary Runs Analysis. Aggregation of symptomatic grapevines was found down the row more often than across the row. The rapid rate of Disease progress and mortality rate of vines in this vineyard suggest that vine-to-vine spread is occurring and that Viognier vines are highly susceptibly to PD

Zhou C. H. - One of the best experts on this subject based on the ideXlab platform.

  • Citrus and coffee strains of Xylella fastidiosa induce Pierce's Disease in grapevine
    Amer Phytopathological Soc, 2002
    Co-Authors: Li W. B., Zhou C. H., Pria W. D., Teixeira D. C., Miranda V. S., Pereira E. O., Ayres A. J., Hartung J. S.
    Abstract:

    Xylella fastidiosa causes citrus variegated chlorosis (CVC) Disease in Brazil and Pierce's Disease of grapevines in the United States. Both of these Diseases cause significant production problems in the respective industries. The recent establishment of the glassy-winged sharpshooter in California has radically increased the threat posed by Pierces Disease to California viticulture. Populations of this insect reach very high levels in citrus groves in California and move from the orchards into the vineyards, where they acquire inoculum and spread Pierce's Disease in the vineyards. Here we show that strains of X. fastidiosa isolated from Diseased citrus and coffee in Brazil can incite symptoms of Pierce's Disease after mechanical inoculation into seven commercial Vitis vinifera varieties grown in Brazil and California. Thus, any future introduction of the CVC strains of X. fastidiosa into the United States would pose a threat to both the sweet orange and grapevine industries. Previous work has clearly shown that the strains of X. fastidiosa isolated from Pierce's Disease- and CVC-affected plants are the most distantly related of all strains in the diverse taxon X. fastidiosa. The ability of citrus strains of X. fastidiosa to incite Disease in grapevine is therefore surprising and creates an experimental system with which to dissect mechanisms used by X.,fastidiosa in plant colonization and Disease development using the full genome sequence data that has recently become available for both the citrus and grapevine strains of this pathogen

Pria W. D. - One of the best experts on this subject based on the ideXlab platform.

  • Citrus and coffee strains of Xylella fastidiosa induce Pierce's Disease in grapevine
    Amer Phytopathological Soc, 2002
    Co-Authors: Li W. B., Zhou C. H., Pria W. D., Teixeira D. C., Miranda V. S., Pereira E. O., Ayres A. J., Hartung J. S.
    Abstract:

    Xylella fastidiosa causes citrus variegated chlorosis (CVC) Disease in Brazil and Pierce's Disease of grapevines in the United States. Both of these Diseases cause significant production problems in the respective industries. The recent establishment of the glassy-winged sharpshooter in California has radically increased the threat posed by Pierces Disease to California viticulture. Populations of this insect reach very high levels in citrus groves in California and move from the orchards into the vineyards, where they acquire inoculum and spread Pierce's Disease in the vineyards. Here we show that strains of X. fastidiosa isolated from Diseased citrus and coffee in Brazil can incite symptoms of Pierce's Disease after mechanical inoculation into seven commercial Vitis vinifera varieties grown in Brazil and California. Thus, any future introduction of the CVC strains of X. fastidiosa into the United States would pose a threat to both the sweet orange and grapevine industries. Previous work has clearly shown that the strains of X. fastidiosa isolated from Pierce's Disease- and CVC-affected plants are the most distantly related of all strains in the diverse taxon X. fastidiosa. The ability of citrus strains of X. fastidiosa to incite Disease in grapevine is therefore surprising and creates an experimental system with which to dissect mechanisms used by X.,fastidiosa in plant colonization and Disease development using the full genome sequence data that has recently become available for both the citrus and grapevine strains of this pathogen