The Experts below are selected from a list of 99030 Experts worldwide ranked by ideXlab platform

Anand K Deva - One of the best experts on this subject based on the ideXlab platform.

Martin Meuli - One of the best experts on this subject based on the ideXlab platform.

  • skingineering i engineering porcine dermo epidermal skin analogues for autologous transplantation in a large animal model
    Pediatric Surgery International, 2011
    Co-Authors: Erik Braziulis, Thomas Biedermann, Fabienne Hartmannfritsch, Clemens Schiestl, Luca Pontiggia, Sophie Bottcherhaberzeth, Ernst Reichmann, Martin Meuli
    Abstract:

    Background Extended full thickness skin defects still represent a considerable therapeutic challenge as ideal strategies for definitive autologous coverage are still not available. Tissue engineering of whole skin represents an equally attractive and ambitious novel approach. We have recently shown that laboratory-grown human skin analogues with near normal skin anatomy can be successfully transplanted on immuno-incompetent rats. The goal of the present Study was to engineer autologous porcine skin grafts for transplantation in a large animal model (Pig Study = intended preclinical Study).

  • Skingineering I: engineering porcine dermo-epidermal skin analogues for autologous transplantation in a large animal model
    Pediatric Surgery International, 2011
    Co-Authors: Erik Braziulis, Thomas Biedermann, Clemens Schiestl, Luca Pontiggia, Ernst Reichmann, Fabienne Hartmann-fritsch, Sophie Böttcher-haberzeth, Martin Meuli
    Abstract:

    Background Extended full thickness skin defects still represent a considerable therapeutic challenge as ideal strategies for definitive autologous coverage are still not available. Tissue engineering of whole skin represents an equally attractive and ambitious novel approach. We have recently shown that laboratory-grown human skin analogues with near normal skin anatomy can be successfully transplanted on immuno-incompetent rats. The goal of the present Study was to engineer autologous porcine skin grafts for transplantation in a large animal model (Pig Study = intended preclinical Study). Materials and methods Skin biopsies were taken from the Pig’s abdomen. Epidermal keratinocytes and dermal fibroblasts were isolated and then expanded on culture dishes. Subsequently, highly concentrated collagen hydrogels and collagen/fibrin hydrogels respectively, both containing dermal fibroblasts, were prepared. Fibroblast survival, proliferation, and morphology were monitored using fluorescent labelling and laser scanning confocal microscopy. Finally, keratinocytes were seeded onto this dermal construct and allowed to proliferate. The resulting in vitro generated porcine skin substitutes were analysed by H&E staining and immunofluorescence. Results Dermal fibroblast proliferation and survival in pure collagen hydrogels was poor. Also, the cells were mainly round-shaped and they did not develop 3D-networks. In collagen/fibrin hydrogels, dermal fibroblast survival was significantly higher. The cells proliferated well, were spindle-shaped, and formed 3D-networks. When these latter dermal constructs were seeded with keratinocytes, a multilayered and partly stratified epidermis readily developed. Conclusion This Study provides compelling evidence that Pig cell-derived skin analogues with near normal skin anatomy can be engineered in vitro. These tissue-engineered skin substitutes are needed to develop a large animal model to establish standardized autologous transplantation procedures for those studies that must be conducted before “skingineering” can eventually be clinically applied.

Juliane Floury - One of the best experts on this subject based on the ideXlab platform.

  • Whey-based cheese provides more postprandial plasma leucine than casein-based cheese: A Pig Study
    Food Chemistry, 2019
    Co-Authors: Lucie Lorieau, Yann Le Gouar, Gwenaele Henry, Tingting Mao, Amandine Ligneul, Etienne Hazart, Didier Dupont, Juliane Floury
    Abstract:

    With a long-term nutrition goal for healthy aging, the aim of this Study was to compare the bioavailability of amino acids, in particular the leucine, after the ingestion of two solid and isocaloric dairy products (cheese) based either on whey or on caseins, by using Pig as an in vivo digestion model. The whey-based cheese contained 25% more leucine than Mozzarella, however its digestion by Pigs resulted in a concentration of postprandial plasma leucine between 2 h and 5 h30 twice higher than that produced during the digestion of Mozzarella. Noting that the dry matter of the duodenal effluents were similar after each of the two cheese meals, differences in gastric emptying would not explain the difference in leucine bioavailability. These results suggest the possibility of stimulating more efficiently the muscle synthesis in elderly people with cheese based on whey proteins rather than those based on caseins.

Anita Jacombs - One of the best experts on this subject based on the ideXlab platform.

Erik Braziulis - One of the best experts on this subject based on the ideXlab platform.

  • skingineering i engineering porcine dermo epidermal skin analogues for autologous transplantation in a large animal model
    Pediatric Surgery International, 2011
    Co-Authors: Erik Braziulis, Thomas Biedermann, Fabienne Hartmannfritsch, Clemens Schiestl, Luca Pontiggia, Sophie Bottcherhaberzeth, Ernst Reichmann, Martin Meuli
    Abstract:

    Background Extended full thickness skin defects still represent a considerable therapeutic challenge as ideal strategies for definitive autologous coverage are still not available. Tissue engineering of whole skin represents an equally attractive and ambitious novel approach. We have recently shown that laboratory-grown human skin analogues with near normal skin anatomy can be successfully transplanted on immuno-incompetent rats. The goal of the present Study was to engineer autologous porcine skin grafts for transplantation in a large animal model (Pig Study = intended preclinical Study).

  • Skingineering I: engineering porcine dermo-epidermal skin analogues for autologous transplantation in a large animal model
    Pediatric Surgery International, 2011
    Co-Authors: Erik Braziulis, Thomas Biedermann, Clemens Schiestl, Luca Pontiggia, Ernst Reichmann, Fabienne Hartmann-fritsch, Sophie Böttcher-haberzeth, Martin Meuli
    Abstract:

    Background Extended full thickness skin defects still represent a considerable therapeutic challenge as ideal strategies for definitive autologous coverage are still not available. Tissue engineering of whole skin represents an equally attractive and ambitious novel approach. We have recently shown that laboratory-grown human skin analogues with near normal skin anatomy can be successfully transplanted on immuno-incompetent rats. The goal of the present Study was to engineer autologous porcine skin grafts for transplantation in a large animal model (Pig Study = intended preclinical Study). Materials and methods Skin biopsies were taken from the Pig’s abdomen. Epidermal keratinocytes and dermal fibroblasts were isolated and then expanded on culture dishes. Subsequently, highly concentrated collagen hydrogels and collagen/fibrin hydrogels respectively, both containing dermal fibroblasts, were prepared. Fibroblast survival, proliferation, and morphology were monitored using fluorescent labelling and laser scanning confocal microscopy. Finally, keratinocytes were seeded onto this dermal construct and allowed to proliferate. The resulting in vitro generated porcine skin substitutes were analysed by H&E staining and immunofluorescence. Results Dermal fibroblast proliferation and survival in pure collagen hydrogels was poor. Also, the cells were mainly round-shaped and they did not develop 3D-networks. In collagen/fibrin hydrogels, dermal fibroblast survival was significantly higher. The cells proliferated well, were spindle-shaped, and formed 3D-networks. When these latter dermal constructs were seeded with keratinocytes, a multilayered and partly stratified epidermis readily developed. Conclusion This Study provides compelling evidence that Pig cell-derived skin analogues with near normal skin anatomy can be engineered in vitro. These tissue-engineered skin substitutes are needed to develop a large animal model to establish standardized autologous transplantation procedures for those studies that must be conducted before “skingineering” can eventually be clinically applied.