The Experts below are selected from a list of 15129 Experts worldwide ranked by ideXlab platform
Eric Delwart - One of the best experts on this subject based on the ideXlab platform.
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viral nucleic acids in live attenuated vaccines detection of minority variants and an adventitious Virus
Journal of Virology, 2010Co-Authors: Joseph Victoria, Chunlin Wang, Morris S Jones, Crystal Jaing, Kevin Mcloughlin, Shea N Gardner, Eric DelwartAbstract:Received 22 December 2009/Accepted 25 March 2010 Metagenomics and a panmicrobial microarray were used to examine eight live-attenuated viral vaccines. Viral nucleic acids in trivalent oral polioVirus (OPV), rubella, measles, yellow fever, varicella-zoster, multivalent measles/mumps/rubella, and two rotaVirus live vaccines were partially purified, randomly amplified, and pyrosequenced. Over half a million sequence reads were generated covering from 20 to 99% of the attenuated viral genomes at depths reaching up to 8,000 reads per nucleotides. Mutations and minority variants, relative to vaccine strains, not known to affect attenuation were detected in OPV, mumps Virus, and varicella-zoster Virus. The anticipated detection of endogenous retroviral sequences from the producer avian and primate cells was confirmed. Avian leukosis Virus (ALV), previously shown to be noninfectious for humans, was present as RNA in viral particles, while simian retroVirus (SRV) was present as genetically defective DNA. Rotarix, an orally administered rotaVirus vaccine, contained porcine circoVirus-1 (PCV1), a highly prevalent nonpathogenic Pig Virus, which has not been shown to be infectious in humans. Hybridization of vaccine nucleic acids to a panmicrobial microarray confirmed the presence of endogenous retroviral and PCV1 nucleic acids. Deep sequencing and microarrays can therefore detect attenuated Virus sequence changes, minority variants, and adventitious Viruses and help maintain the current safety record of live-attenuated viral vaccines.
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Viral Nucleic Acids in Live-Attenuated Vaccines: Detection of Minority Variants and an Adventitious Virus†
2009Co-Authors: Shea Gardner, Eric DelwartAbstract:Metagenomics and a panmicrobial microarray were used to examine eight live-attenuated viral vaccines. Viral nucleic acids in trivalent oral polioVirus (OPV), rubella, measles, yellow fever, varicella-zoster, multiva-lent measles/mumps/rubella, and two rotaVirus live vaccines were partially purified, randomly amplified, and pyrosequenced. Over half a million sequence reads were generated covering from 20 to 99 % of the attenuated viral genomes at depths reaching up to 8,000 reads per nucleotides. Mutations and minority variants, relative to vaccine strains, not known to affect attenuation were detected in OPV, mumps Virus, and varicella-zoster Virus. The anticipated detection of endogenous retroviral sequences from the producer avian and primate cells was confirmed. Avian leukosis Virus (ALV), previously shown to be noninfectious for humans, was present as RNA in viral particles, while simian retroVirus (SRV) was present as genetically defective DNA. Rotarix, an orally administered rotaVirus vaccine, contained porcine circoVirus-1 (PCV1), a highly prevalent nonpatho-genic Pig Virus, which has not been shown to be infectious in humans. Hybridization of vaccine nucleic acids to a panmicrobial microarray confirmed the presence of endogenous retroviral and PCV1 nucleic acids. Deep sequencing and microarrays can therefore detect attenuated Virus sequence changes, minority variants, and adventitious Viruses and help maintain the current safety record of live-attenuated viral vaccines. Highly effective, safe, and relatively inexpensive, live-atten
Joseph Victoria - One of the best experts on this subject based on the ideXlab platform.
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viral nucleic acids in live attenuated vaccines detection of minority variants and an adventitious Virus
Journal of Virology, 2010Co-Authors: Joseph Victoria, Chunlin Wang, Morris S Jones, Crystal Jaing, Kevin Mcloughlin, Shea N Gardner, Eric DelwartAbstract:Received 22 December 2009/Accepted 25 March 2010 Metagenomics and a panmicrobial microarray were used to examine eight live-attenuated viral vaccines. Viral nucleic acids in trivalent oral polioVirus (OPV), rubella, measles, yellow fever, varicella-zoster, multivalent measles/mumps/rubella, and two rotaVirus live vaccines were partially purified, randomly amplified, and pyrosequenced. Over half a million sequence reads were generated covering from 20 to 99% of the attenuated viral genomes at depths reaching up to 8,000 reads per nucleotides. Mutations and minority variants, relative to vaccine strains, not known to affect attenuation were detected in OPV, mumps Virus, and varicella-zoster Virus. The anticipated detection of endogenous retroviral sequences from the producer avian and primate cells was confirmed. Avian leukosis Virus (ALV), previously shown to be noninfectious for humans, was present as RNA in viral particles, while simian retroVirus (SRV) was present as genetically defective DNA. Rotarix, an orally administered rotaVirus vaccine, contained porcine circoVirus-1 (PCV1), a highly prevalent nonpathogenic Pig Virus, which has not been shown to be infectious in humans. Hybridization of vaccine nucleic acids to a panmicrobial microarray confirmed the presence of endogenous retroviral and PCV1 nucleic acids. Deep sequencing and microarrays can therefore detect attenuated Virus sequence changes, minority variants, and adventitious Viruses and help maintain the current safety record of live-attenuated viral vaccines.
Kevin Mcloughlin - One of the best experts on this subject based on the ideXlab platform.
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viral nucleic acids in live attenuated vaccines detection of minority variants and an adventitious Virus
Journal of Virology, 2010Co-Authors: Joseph Victoria, Chunlin Wang, Morris S Jones, Crystal Jaing, Kevin Mcloughlin, Shea N Gardner, Eric DelwartAbstract:Received 22 December 2009/Accepted 25 March 2010 Metagenomics and a panmicrobial microarray were used to examine eight live-attenuated viral vaccines. Viral nucleic acids in trivalent oral polioVirus (OPV), rubella, measles, yellow fever, varicella-zoster, multivalent measles/mumps/rubella, and two rotaVirus live vaccines were partially purified, randomly amplified, and pyrosequenced. Over half a million sequence reads were generated covering from 20 to 99% of the attenuated viral genomes at depths reaching up to 8,000 reads per nucleotides. Mutations and minority variants, relative to vaccine strains, not known to affect attenuation were detected in OPV, mumps Virus, and varicella-zoster Virus. The anticipated detection of endogenous retroviral sequences from the producer avian and primate cells was confirmed. Avian leukosis Virus (ALV), previously shown to be noninfectious for humans, was present as RNA in viral particles, while simian retroVirus (SRV) was present as genetically defective DNA. Rotarix, an orally administered rotaVirus vaccine, contained porcine circoVirus-1 (PCV1), a highly prevalent nonpathogenic Pig Virus, which has not been shown to be infectious in humans. Hybridization of vaccine nucleic acids to a panmicrobial microarray confirmed the presence of endogenous retroviral and PCV1 nucleic acids. Deep sequencing and microarrays can therefore detect attenuated Virus sequence changes, minority variants, and adventitious Viruses and help maintain the current safety record of live-attenuated viral vaccines.
Shea N Gardner - One of the best experts on this subject based on the ideXlab platform.
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viral nucleic acids in live attenuated vaccines detection of minority variants and an adventitious Virus
Journal of Virology, 2010Co-Authors: Joseph Victoria, Chunlin Wang, Morris S Jones, Crystal Jaing, Kevin Mcloughlin, Shea N Gardner, Eric DelwartAbstract:Received 22 December 2009/Accepted 25 March 2010 Metagenomics and a panmicrobial microarray were used to examine eight live-attenuated viral vaccines. Viral nucleic acids in trivalent oral polioVirus (OPV), rubella, measles, yellow fever, varicella-zoster, multivalent measles/mumps/rubella, and two rotaVirus live vaccines were partially purified, randomly amplified, and pyrosequenced. Over half a million sequence reads were generated covering from 20 to 99% of the attenuated viral genomes at depths reaching up to 8,000 reads per nucleotides. Mutations and minority variants, relative to vaccine strains, not known to affect attenuation were detected in OPV, mumps Virus, and varicella-zoster Virus. The anticipated detection of endogenous retroviral sequences from the producer avian and primate cells was confirmed. Avian leukosis Virus (ALV), previously shown to be noninfectious for humans, was present as RNA in viral particles, while simian retroVirus (SRV) was present as genetically defective DNA. Rotarix, an orally administered rotaVirus vaccine, contained porcine circoVirus-1 (PCV1), a highly prevalent nonpathogenic Pig Virus, which has not been shown to be infectious in humans. Hybridization of vaccine nucleic acids to a panmicrobial microarray confirmed the presence of endogenous retroviral and PCV1 nucleic acids. Deep sequencing and microarrays can therefore detect attenuated Virus sequence changes, minority variants, and adventitious Viruses and help maintain the current safety record of live-attenuated viral vaccines.
Crystal Jaing - One of the best experts on this subject based on the ideXlab platform.
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viral nucleic acids in live attenuated vaccines detection of minority variants and an adventitious Virus
Journal of Virology, 2010Co-Authors: Joseph Victoria, Chunlin Wang, Morris S Jones, Crystal Jaing, Kevin Mcloughlin, Shea N Gardner, Eric DelwartAbstract:Received 22 December 2009/Accepted 25 March 2010 Metagenomics and a panmicrobial microarray were used to examine eight live-attenuated viral vaccines. Viral nucleic acids in trivalent oral polioVirus (OPV), rubella, measles, yellow fever, varicella-zoster, multivalent measles/mumps/rubella, and two rotaVirus live vaccines were partially purified, randomly amplified, and pyrosequenced. Over half a million sequence reads were generated covering from 20 to 99% of the attenuated viral genomes at depths reaching up to 8,000 reads per nucleotides. Mutations and minority variants, relative to vaccine strains, not known to affect attenuation were detected in OPV, mumps Virus, and varicella-zoster Virus. The anticipated detection of endogenous retroviral sequences from the producer avian and primate cells was confirmed. Avian leukosis Virus (ALV), previously shown to be noninfectious for humans, was present as RNA in viral particles, while simian retroVirus (SRV) was present as genetically defective DNA. Rotarix, an orally administered rotaVirus vaccine, contained porcine circoVirus-1 (PCV1), a highly prevalent nonpathogenic Pig Virus, which has not been shown to be infectious in humans. Hybridization of vaccine nucleic acids to a panmicrobial microarray confirmed the presence of endogenous retroviral and PCV1 nucleic acids. Deep sequencing and microarrays can therefore detect attenuated Virus sequence changes, minority variants, and adventitious Viruses and help maintain the current safety record of live-attenuated viral vaccines.