The Experts below are selected from a list of 17292 Experts worldwide ranked by ideXlab platform
Claire C. Bastie - One of the best experts on this subject based on the ideXlab platform.
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elevated fetal adipsin acylation stimulating protein asp in obese pregnancy novel Placental secretion via hofbauer cells
The Journal of Clinical Endocrinology and Metabolism, 2013Co-Authors: K. Sivakumar, Muhammad Furqan Bari, Antonysunil Adaikalakoteswari, S. Guller, Martin O. Weickert, Harpal S. Randeva, Dimitris K. Grammatopoulos, Claire C. Bastie, Manu VatishAbstract:Context and Objective: Obesity in pregnancy is associated with increased risks of obesity in the offspring. We investigated the relationship between obesity in pregnancy and circulating maternal and fetal levels of adipose Tissue-derived factors adipsin and acylation stimulating protein (ASP) in lean and obese mothers. Design: Paired peripheral and cord blood samples were taken. Paired fat and Placenta Tissue were taken for explant culture. Media were assayed for secreted adipsin and ASP. Clinical parameters assayed included fasting insulin, glucose, and adipsin. Setting: The study was conducted at a university hospital maternity unit. Patients: Patients included 35 lean [body mass index (BMI) 19–25 kg/m2, mean age 32 years and 39 obese (BMI) > 30 kg/m2, mean age 32.49 years] pregnant Caucasian women, delivered by cesarean section at term. Main Outcome Measure: Identification of Placental macrophages [Hofbauer cells (HBCs)], as a source of adipsin and ASP was determined. Results: HBCs secreted both adipsi...
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Elevated fetal adipsin/acylation-stimulating protein (ASP) in obese pregnancy: novel Placental secretion via Hofbauer cells
'The Endocrine Society', 2013Co-Authors: Sivakumar K, Martin O. Weickert, Harpal S. Randeva, Dimitris K. Grammatopoulos, Claire C. Bastie, Mf Bari, Adaikalakoteswari A, Guller S, Vatish MAbstract:Context and Objective: Obesity in pregnancy is associated with increased risks of obesity in the offspring. We investigated the relationship between obesity in pregnancy and circulating maternal and fetal levels of adipose Tissue-derived factors adipsin and acylation stimulating protein (ASP) in lean and obese mothers. Design: Paired peripheral and cord blood samples were taken. Paired fat and Placenta Tissue were taken for explant culture. Media were assayed for secreted adipsin and ASP. Clinical parameters assayed included fasting insulin, glucose, and adipsin. Setting: The study was conducted at a university hospital maternity unit. Patients: Patients included 35 lean [body mass index (BMI) 19–25 kg/m2, mean age 32 years and 39 obese (BMI) > 30 kg/m2, mean age 32.49 years] pregnant Caucasian women, delivered by cesarean section at term. Main Outcome Measure: Identification of Placental macrophages [Hofbauer cells (HBCs)], as a source of adipsin and ASP was determined. Results: HBCs secreted both adipsin and ASP. Cord levels of adipsin (1663.78 ± 52.76 pg/mL) and ASP (354.48 ± 17.17 ng/mL) were significantly elevated in the offspring of obese mothers compared with their lean controls [1354.66 ± 33.87 pg/mL and 302.63 ± 14.98 ng/mL, respectively (P < .05 for both)]. Placentae from obese mothers released significantly more adipsin and ASP than Placentae from lean mothers [546.0 ± 44 pg/mL·g vs 284.56 ± 43 pg/mL·g and 5485.75 ± 163.32 ng/mL·g vs 2399.16 ± 181.83 ng/mL·g, respectively (P < .05 for both)]. Circulating fetal adipsin and ASP positively correlated with maternal BMI (r = 0.611, P < .0001, and r = 0.391, P < .05, respectively). Fetal adipsin correlated positively with maternal (r = 0.482, P < .01) and fetal homeostasis model assessment of insulin resistance (r = 0.465, P < .01). Conclusions: We demonstrate novel secretion of adipsin and ASP by Placental HBCs
Fei Zhong - One of the best experts on this subject based on the ideXlab platform.
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porcine parvovirus infection causes pig Placenta Tissue damage involving nonstructural protein 1 ns1 induced intrinsic ros mitochondria mediated apoptosis
Viruses, 2019Co-Authors: Jianlou Zhang, Jinghui Fan, Shuang Liang, Shanshan Huo, Xing Wang, Yuzhu Zuo, Dan Cui, Zhenyu Zhong, Fei ZhongAbstract:Porcine parvovirus (PPV) is an important pathogen causing reproductive failure in pigs. PPV-induced cell apoptosis has been recently identified as being involved in PPV-induced Placental Tissue damages resulting in reproductive failure. However, the molecular mechanism was not fully elucidated. Here we demonstrate that PPV nonstructural protein 1 (NS1) can induce host cell apoptosis and death, thereby indicating the NS1 may play a crucial role in PPV-induced Placental Tissue damages and reproductive failure. We have found that NS1-induced apoptosis was significantly inhibited by caspase 9 inhibitor, but not caspase 8 inhibitor, and transfection of NS1 gene into PK-15 cells significantly inhibited mitochondria-associated antiapoptotic molecules Bcl-2 and Mcl-1 expressions and enhanced proapoptotic molecules Bax, P21, and P53 expressions, suggesting that NS1-induced apoptosis is mainly through the mitochondria-mediated intrinsic apoptosis pathway. We also found that both PPV infection and NS1 vector transfection could cause host DNA damage resulting in cell cycle arrest at the G1 and G2 phases, trigger mitochondrial ROS accumulation resulting in mitochondria damage, and therefore, induce the host cell apoptosis. This study provides a molecular basis for elucidating PPV-induced cell apoptosis and reproductive failure.
Manu Vatish - One of the best experts on this subject based on the ideXlab platform.
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elevated fetal adipsin acylation stimulating protein asp in obese pregnancy novel Placental secretion via hofbauer cells
The Journal of Clinical Endocrinology and Metabolism, 2013Co-Authors: K. Sivakumar, Muhammad Furqan Bari, Antonysunil Adaikalakoteswari, S. Guller, Martin O. Weickert, Harpal S. Randeva, Dimitris K. Grammatopoulos, Claire C. Bastie, Manu VatishAbstract:Context and Objective: Obesity in pregnancy is associated with increased risks of obesity in the offspring. We investigated the relationship between obesity in pregnancy and circulating maternal and fetal levels of adipose Tissue-derived factors adipsin and acylation stimulating protein (ASP) in lean and obese mothers. Design: Paired peripheral and cord blood samples were taken. Paired fat and Placenta Tissue were taken for explant culture. Media were assayed for secreted adipsin and ASP. Clinical parameters assayed included fasting insulin, glucose, and adipsin. Setting: The study was conducted at a university hospital maternity unit. Patients: Patients included 35 lean [body mass index (BMI) 19–25 kg/m2, mean age 32 years and 39 obese (BMI) > 30 kg/m2, mean age 32.49 years] pregnant Caucasian women, delivered by cesarean section at term. Main Outcome Measure: Identification of Placental macrophages [Hofbauer cells (HBCs)], as a source of adipsin and ASP was determined. Results: HBCs secreted both adipsi...
Jianlou Zhang - One of the best experts on this subject based on the ideXlab platform.
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porcine parvovirus infection causes pig Placenta Tissue damage involving nonstructural protein 1 ns1 induced intrinsic ros mitochondria mediated apoptosis
Viruses, 2019Co-Authors: Jianlou Zhang, Jinghui Fan, Shuang Liang, Shanshan Huo, Xing Wang, Yuzhu Zuo, Dan Cui, Zhenyu Zhong, Fei ZhongAbstract:Porcine parvovirus (PPV) is an important pathogen causing reproductive failure in pigs. PPV-induced cell apoptosis has been recently identified as being involved in PPV-induced Placental Tissue damages resulting in reproductive failure. However, the molecular mechanism was not fully elucidated. Here we demonstrate that PPV nonstructural protein 1 (NS1) can induce host cell apoptosis and death, thereby indicating the NS1 may play a crucial role in PPV-induced Placental Tissue damages and reproductive failure. We have found that NS1-induced apoptosis was significantly inhibited by caspase 9 inhibitor, but not caspase 8 inhibitor, and transfection of NS1 gene into PK-15 cells significantly inhibited mitochondria-associated antiapoptotic molecules Bcl-2 and Mcl-1 expressions and enhanced proapoptotic molecules Bax, P21, and P53 expressions, suggesting that NS1-induced apoptosis is mainly through the mitochondria-mediated intrinsic apoptosis pathway. We also found that both PPV infection and NS1 vector transfection could cause host DNA damage resulting in cell cycle arrest at the G1 and G2 phases, trigger mitochondrial ROS accumulation resulting in mitochondria damage, and therefore, induce the host cell apoptosis. This study provides a molecular basis for elucidating PPV-induced cell apoptosis and reproductive failure.
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Porcine Parvovirus Infection Causes Pig Placenta Tissue Damage Involving Nonstructural Protein 1 (NS1)-Induced Intrinsic ROS/Mitochondria-Mediated Apoptosis
MDPI AG, 2019Co-Authors: Jianlou Zhang, Jinghui Fan, Shuang Liang, Shanshan Huo, Xing Wang, Yuzhu Zuo, Dan Cui, Zhenyu ZhongAbstract:Porcine parvovirus (PPV) is an important pathogen causing reproductive failure in pigs. PPV-induced cell apoptosis has been recently identified as being involved in PPV-induced Placental Tissue damages resulting in reproductive failure. However, the molecular mechanism was not fully elucidated. Here we demonstrate that PPV nonstructural protein 1 (NS1) can induce host cell apoptosis and death, thereby indicating the NS1 may play a crucial role in PPV-induced Placental Tissue damages and reproductive failure. We have found that NS1-induced apoptosis was significantly inhibited by caspase 9 inhibitor, but not caspase 8 inhibitor, and transfection of NS1 gene into PK-15 cells significantly inhibited mitochondria-associated antiapoptotic molecules Bcl-2 and Mcl-1 expressions and enhanced proapoptotic molecules Bax, P21, and P53 expressions, suggesting that NS1-induced apoptosis is mainly through the mitochondria-mediated intrinsic apoptosis pathway. We also found that both PPV infection and NS1 vector transfection could cause host DNA damage resulting in cell cycle arrest at the G1 and G2 phases, trigger mitochondrial ROS accumulation resulting in mitochondria damage, and therefore, induce the host cell apoptosis. This study provides a molecular basis for elucidating PPV-induced cell apoptosis and reproductive failure
Karin B Michels - One of the best experts on this subject based on the ideXlab platform.
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locus specific dna methylation in the Placenta is associated with levels of pro inflammatory proteins in cord blood and they are both independently affected by maternal smoking during pregnancy
Epigenetics, 2017Co-Authors: Sanne D Van Otterdijk, Alexandra M Binder, Karin B MichelsAbstract:ABSTRACTWe investigated the impact of maternal smoking during pregnancy on Placental DNA methylation and how this may mediate the association between maternal smoking and pro-inflammatory proteins in cord blood. The study population consisted of 27 individuals exposed to maternal smoking throughout pregnancy, 32 individuals exposed during a proportion of the pregnancy, and 61 unexposed individuals. Methylation of 11 regions within 6 genes in Placenta Tissue was assessed by pyrosequencing. Levels of 7 pro-inflammatory proteins in cord blood were assessed by electrochemiluminescence. Differential methylation was observed in the CYP1A1 promoter and AHRR gene body regions between women who smoked throughout pregnancy and non-smokers on the fetal-side of the Placenta and in the GFI1 promoter between women who quit smoking while pregnant and non-smokers on the maternal-side of the Placenta. Maternal smoking resulted in elevated levels of IL-8 protein in cord blood, which was not mediated by DNA methylation of o...