The Experts below are selected from a list of 234885 Experts worldwide ranked by ideXlab platform
Dominique Cabrol - One of the best experts on this subject based on the ideXlab platform.
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radiofrequency ablation of retained Placenta accreta after conservative management preliminary evaluation in the pregnant ewe and in normal human Placenta in vitro
British Journal of Obstetrics and Gynaecology, 2009Co-Authors: Olivier Morel, E Monceau, N Tran, C Malartic, F Morel, Emmanuel Barranger, J F Cote, Etienne Gayat, Pascale Chavattepalmer, Dominique CabrolAbstract:Objective To evaluate radiofrequency (RF) efficiency and safety for the ablation of retained Placenta in humans, using a pregnant sheep model. Design Experimental study. Setting Laboratory of Surgery School, Nancy, France. Population/sample Three pregnant ewes/ten human Placentas. Methods Various RF procedures were tested in pregnant ewes on 50 placentomes (individual Placental units). Reproducibility of the best procedure was then evaluated in a further 20 placentomes and on ten human term Placentas in vitro after delivery. Main outcome measures Placental tissues destruction, lesions’ size, myometrial lesions. Results Low power (100 W) and low target temperatures (60°C) lead to homogenous tissue destruction, without myometrial lesion. No significant difference was observed in terms of lesion size and procedure duration for in the placentomes of pregnant ewe in vivo and in human Placentas in vitro. The diameter of the ablation could be correlated with the tines deployment. Conclusion The Placental tissue structure is very permissive to RF energy, which suggests that RF could be used for the ablation of retained Placenta, providing optimal control of tissue destruction. These results call for further experimental evaluations.
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Radiofrequency ablation of retained Placenta accreta after conservative management: preliminary evaluation in the pregnant ewe and in normal human Placenta in vitro
BJOG: An International Journal of Obstetrics and Gynaecology, 2009Co-Authors: Olivier Morel, E Monceau, N Tran, C Malartic, F Morel, Emmanuel Barranger, J F Cote, Etienne Gayat, Pascale Chavatte Palmer, Dominique CabrolAbstract:OBJECTIVE: To evaluate radiofrequency (RF) efficiency and safety for the ablation of retained Placenta in humans, using a pregnant sheep model. DESIGN: Experimental study. SETTING: Laboratory of Surgery School, Nancy, France. POPULATION/SAMPLE: Three pregnant ewes/ten human Placentas. METHODS: Various RF procedures were tested in pregnant ewes on 50 placentomes (individual Placental units). Reproducibility of the best procedure was then evaluated in a further 20 placentomes and on ten human term Placentas in vitro after delivery. MAIN OUTCOME MEASURES: Placental tissues destruction, lesions' size, myometrial lesions. RESULTS: Low power (100 W) and low target temperatures (60 degrees C) lead to homogenous tissue destruction, without myometrial lesion. No significant difference was observed in terms of lesion size and procedure duration for in the placentomes of pregnant ewe in vivo and in human Placentas in vitro. The diameter of the ablation could be correlated with the tines deployment. CONCLUSION: The Placental tissue structure is very permissive to RF energy, which suggests that RF could be used for the ablation of retained Placenta, providing optimal control of tissue destruction. These results call for further experimental evaluations.
Cheryl S Rosenfeld - One of the best experts on this subject based on the ideXlab platform.
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sex specific Placental responses in fetal development
Endocrinology, 2015Co-Authors: Cheryl S RosenfeldAbstract:The Placenta is an ephemeral but critical organ for the survival of all eutherian mammals and marsupials. It is the primary messenger system between the mother and fetus, where communicational signals, nutrients, waste, gases, and extrinsic factors are exchanged. Although the Placenta may buffer the fetus from various environmental insults, Placental dysfunction might also contribute to detrimental developmental origins of adult health and disease effects. The Placenta of one sex over the other might possess greater ability to respond and buffer against environmental insults. Given the potential role of the Placenta in effecting the lifetime health of the offspring, it is not surprising that there has been a resurging interest in this organ, including the Human Placental Project launched by the National Institutes of Child Health and Human Development. In this review, we will compare embryological development of the laboratory mouse and human chorioallantoic Placentae. Next, evidence that various species, including humans, exhibit normal sex-dependent structural and functional Placental differences will be examined followed by how in utero environmental changes (nutritional state, stress, and exposure to environmental chemicals) might interact with fetal sex to affect this organ. Recent data also suggest that paternal state impacts Placental function in a sex-dependent manner. The research to date linking Placental maladaptive responses and later developmental origins of adult health and disease effects will be explored. Finally, we will focus on how sex chromosomes and epimutations may contribute to sex-dependent differences in Placental function, the unanswered questions, and future directions that warrant further consideration.
Sandra T Davidge - One of the best experts on this subject based on the ideXlab platform.
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sex specific effects of nanoparticle encapsulated mitoq nmitoq delivery to the Placenta in a rat model of fetal hypoxia
Frontiers in Physiology, 2019Co-Authors: Esha Ganguly, Mais M Aljunaidy, Jude S Morton, Raven Kirschenman, Thomas Phillips, Patrick C Case, Christylynn M Cooke, Floor Spaans, Sandra T DavidgeAbstract:Pregnancy complications associated with chronic fetal hypoxia have been linked to the development of adult cardiovascular disease in the offspring. Prenatal hypoxia has been shown to increase Placental oxidative stress and impair Placental function in a sex-specific manner, thereby affecting fetal development. As oxidative stress is central to Placental dysfunction, we developed a Placenta-targeted treatment strategy using the antioxidant MitoQ encapsulated into nanoparticles (nMitoQ) to reduce Placental oxidative/nitrosative stress and improve Placental function without direct drug exposure to the fetus in order to avoid off-target effects during development. We hypothesized that, in a rat model of prenatal hypoxia, nMitoQ prevents hypoxia-induced Placental oxidative/nitrosative stress, promotes angiogenesis, improves Placental morphology, and ultimately improves fetal oxygenation. Additionally, we assessed whether there were sex differences in the effectiveness of nMitoQ treatment. Pregnant rats were intravenously injected with saline or nMitoQ (100µl of 125 μM) on gestational day (GD) 15 and exposed to either normoxia (21% O2) or hypoxia (11% O2) from GD15-21. On GD21, Placentae from both sexes were collected for detection of superoxide, nitrotyrosine, nitric oxide, CD31 (endothelial cell marker) and fetal blood spaces, Vegfa and Igf2 mRNA expression in the Placental labyrinth zone. Prenatal hypoxia decreased male fetal weight which was not changed by nMitoQ treatment; however, Placental efficiency (fetal/Placental weight ratio) decreased by hypoxia, and was increased by nMitoQ in both males and females. nMitoQ treatment reduced the prenatal hypoxia-induced increase in Placental superoxide levels in both male and female Placentae but improved oxygenation in only female Placentae. Nitrotyrosine levels were increased in hypoxic female Placentae and were reduced by nMitoQ. Prenatal hypoxia reduced Placental Vegfa and Igf2 expression in both sexes while nMitoQ increased Vegfa and Igf2 expression only in hypoxic female Placentae. In summary, our study suggests that nMitoQ treatment could be pursued as a potential preventative strategy against Placental oxidative stress and programming of adult cardiovascular disease in offspring exposed to hypoxia in utero. However, sex differences need to be taken into account when developing therapeutic strategies to improve fetal development in complicated pregnancies, as nMitoQ treatment was more effective in Placentae from females compared with males.
Olivier Morel - One of the best experts on this subject based on the ideXlab platform.
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radiofrequency ablation of retained Placenta accreta after conservative management preliminary evaluation in the pregnant ewe and in normal human Placenta in vitro
British Journal of Obstetrics and Gynaecology, 2009Co-Authors: Olivier Morel, E Monceau, N Tran, C Malartic, F Morel, Emmanuel Barranger, J F Cote, Etienne Gayat, Pascale Chavattepalmer, Dominique CabrolAbstract:Objective To evaluate radiofrequency (RF) efficiency and safety for the ablation of retained Placenta in humans, using a pregnant sheep model. Design Experimental study. Setting Laboratory of Surgery School, Nancy, France. Population/sample Three pregnant ewes/ten human Placentas. Methods Various RF procedures were tested in pregnant ewes on 50 placentomes (individual Placental units). Reproducibility of the best procedure was then evaluated in a further 20 placentomes and on ten human term Placentas in vitro after delivery. Main outcome measures Placental tissues destruction, lesions’ size, myometrial lesions. Results Low power (100 W) and low target temperatures (60°C) lead to homogenous tissue destruction, without myometrial lesion. No significant difference was observed in terms of lesion size and procedure duration for in the placentomes of pregnant ewe in vivo and in human Placentas in vitro. The diameter of the ablation could be correlated with the tines deployment. Conclusion The Placental tissue structure is very permissive to RF energy, which suggests that RF could be used for the ablation of retained Placenta, providing optimal control of tissue destruction. These results call for further experimental evaluations.
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Radiofrequency ablation of retained Placenta accreta after conservative management: preliminary evaluation in the pregnant ewe and in normal human Placenta in vitro
BJOG: An International Journal of Obstetrics and Gynaecology, 2009Co-Authors: Olivier Morel, E Monceau, N Tran, C Malartic, F Morel, Emmanuel Barranger, J F Cote, Etienne Gayat, Pascale Chavatte Palmer, Dominique CabrolAbstract:OBJECTIVE: To evaluate radiofrequency (RF) efficiency and safety for the ablation of retained Placenta in humans, using a pregnant sheep model. DESIGN: Experimental study. SETTING: Laboratory of Surgery School, Nancy, France. POPULATION/SAMPLE: Three pregnant ewes/ten human Placentas. METHODS: Various RF procedures were tested in pregnant ewes on 50 placentomes (individual Placental units). Reproducibility of the best procedure was then evaluated in a further 20 placentomes and on ten human term Placentas in vitro after delivery. MAIN OUTCOME MEASURES: Placental tissues destruction, lesions' size, myometrial lesions. RESULTS: Low power (100 W) and low target temperatures (60 degrees C) lead to homogenous tissue destruction, without myometrial lesion. No significant difference was observed in terms of lesion size and procedure duration for in the placentomes of pregnant ewe in vivo and in human Placentas in vitro. The diameter of the ablation could be correlated with the tines deployment. CONCLUSION: The Placental tissue structure is very permissive to RF energy, which suggests that RF could be used for the ablation of retained Placenta, providing optimal control of tissue destruction. These results call for further experimental evaluations.
Philip Britz-mckibbin - One of the best experts on this subject based on the ideXlab platform.
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Placental Metabolomics for Assessment of Sex-specific Differences in Fetal Development During Normal Gestation
Scientific Reports, 2020Co-Authors: Michelle Saoi, Katherine M. Kennedy, Wajiha Gohir, Deborah M. Sloboda, Philip Britz-mckibbinAbstract:The Placenta is a metabolically active interfacial organ that plays crucial roles in fetal nutrient delivery, gas exchange and waste removal reflecting dynamic maternal and fetal interactions during gestation. There is growing evidence that the sex of the Placenta influences fetal responses to external stimuli in utero , such as changes in maternal nutrition and exposure to environmental stressors. However, the exact biochemical mechanisms associated with sex-specific metabolic adaptations during pregnancy and its link to Placental function and fetal development remain poorly understood. Herein, multisegment injection-capillary electrophoresis-mass spectrometry is used as a high throughput metabolomics platform to characterize lyophilized Placental tissue (~2 mg dried weight) from C57BL/6J mice fed a standardized diet. Over 130 authentic metabolites were consistently measured from Placental extracts when using a nontargeted metabolomics workflow with stringent quality control and robust batch correction. Our work revealed distinct metabolic phenotype differences that exist between male ( n = 14) and female ( n = 14) Placentae collected at embryonic day E18.5. Intracellular metabolites associated with fatty acid oxidation and purine degradation were found to be elevated in females as compared to male Placentae ( p 0.40), including uric acid, valerylcarnitine, hexanoylcarnitine, and 3-hydroxyhexanolycarnitine. This murine model sheds new insights into sex-specific differences in Placental mitochondrial function and protective mechanisms against deleterious oxidative stress that may impact fetal growth and birth outcomes later in life.
