The Experts below are selected from a list of 16131 Experts worldwide ranked by ideXlab platform

A M Weindling - One of the best experts on this subject based on the ideXlab platform.

  • randomised controlled trial of Plasma Protein Fraction versus dopamine in hypotensive very low birthweight infants
    Archives of Disease in Childhood, 1993
    Co-Authors: A B Gill, A M Weindling
    Abstract:

    Around 20% of very low birthweight infants admitted to a neonatal intensive care unit become hypotensive within 24 hours of their admission. Standard treatment is either expansion of the circulating volume by the infusion of Plasma Protein Fraction or by using dopamine to improve cardiac function. The purpose of this study was to investigate by a randomised controlled trial which was the most appropriate treatment. Thirty nine infants were randomised to receive either Plasma Protein Fraction or dopamine as first line treatment if they became hypotensive within 24 hours of admission to the neonatal intensive care unit. Seventeen of 19 (89%) infants responded to dopamine, whereas only 9/20 (45%) responded to Plasma Protein Fraction. The median dose of dopamine needed to increase the blood pressure to at least the 10th centile was 7.5 micrograms/kg/min and was infused for a median duration of 18 hours. These observations suggest that dopamine should be used earlier in the treatment of these infants than has previously been recommended.

Michael Weindling - One of the best experts on this subject based on the ideXlab platform.

A B Gill - One of the best experts on this subject based on the ideXlab platform.

  • randomised controlled trial of Plasma Protein Fraction versus dopamine in hypotensive very low birthweight infants
    Archives of Disease in Childhood, 1993
    Co-Authors: A B Gill, A M Weindling
    Abstract:

    Around 20% of very low birthweight infants admitted to a neonatal intensive care unit become hypotensive within 24 hours of their admission. Standard treatment is either expansion of the circulating volume by the infusion of Plasma Protein Fraction or by using dopamine to improve cardiac function. The purpose of this study was to investigate by a randomised controlled trial which was the most appropriate treatment. Thirty nine infants were randomised to receive either Plasma Protein Fraction or dopamine as first line treatment if they became hypotensive within 24 hours of admission to the neonatal intensive care unit. Seventeen of 19 (89%) infants responded to dopamine, whereas only 9/20 (45%) responded to Plasma Protein Fraction. The median dose of dopamine needed to increase the blood pressure to at least the 10th centile was 7.5 micrograms/kg/min and was infused for a median duration of 18 hours. These observations suggest that dopamine should be used earlier in the treatment of these infants than has previously been recommended.

Bryan Gill - One of the best experts on this subject based on the ideXlab platform.

Hartmut Schlüter - One of the best experts on this subject based on the ideXlab platform.

  • sample displacement batch chromatography of Proteins
    Methods of Molecular Biology, 2014
    Co-Authors: Marta Kotasinska, Verena Richter, Marcel Kwiatkowski, Hartmut Schlüter
    Abstract:

    In downstream processing, large-scale chromatography plays an important role. For its development, screening experiments followed by pilot-plant chromatography are mandatory steps. Here we describe fast, simple, and inexpensive methods for establishing a preparative chromatography for the separation of complex Protein mixtures, based on sample displacement batch chromatography. The methods are demonstrated by anion-exchange chromatography of a human Plasma Protein Fraction (Cohn IV-4), including the screening step and upscaling of the chromatography by a factor of one hundred. The results of the screening experiments and the preparative chromatography are monitored by SDS-PAGE electrophoresis. In summary, we provide a protocol, which should be easily adaptable for the chromatographic large-scale purification of other Proteins, in the laboratory as well as in the manufacturing of biopharmaceuticals. These protocols cover the initial piloting steps for establishing a large-scale sample batch chromatography. The results from the piloting steps may also be applied for packed columns for performing simulated-moving-bed (SMB) chromatography rather than batch chromatography.

  • Application of displacement chromatography for the proteome analysis of a human Plasma Protein Fraction.
    Journal of chromatography. A, 2009
    Co-Authors: Robert Ahrends, Björn Lichtner, Andreas Bertsch, Oliver Kohlbacher, Diana Hildebrand, Maria Trusch, Hartmut Schlüter
    Abstract:

    It was the aim of this study to compare the performance of displacement chromatography with gradient elution chromatography both applied as the cation-exchange separation step for a proteome analysis in a bottom-up approach using multidimensional chromatography for the separation of tryptic peptides prior to their mass spectrometric analysis. The tryptic digest of the human Cohn Fraction IV-4 served as a sample. For both chromatography modes commonly used operating parameters were chosen thus ensuring optimal separation results of equal sample amounts for each mode. All resulting Fractions were analyzed with an HPLC-chip-LC-MS system. The eluate of the HPLC-chip column was ionized by electrospray ionization (ESI) and analyzed with an ion-trap mass spectrometer. For guaranteeing high confidence concerning the identity of the peptides, the mass spectrometric data were processed by different bioinformatic tools applying stringent criteria. By the displacement approach the total amount of identified Proteins (78) was significantly higher than in the gradient mode (58). The results showed that displacement chromatography is a well suited alternative in comparison to gradient elution separation for analysis of proteomes via the bottom-up approach applying multidimensional chromatography, especially in those cases when larger quantities of Proteins are available.