The Experts below are selected from a list of 48651 Experts worldwide ranked by ideXlab platform
Jian-ding Qiu - One of the best experts on this subject based on the ideXlab platform.
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Target-Triggering Multiple-Cycle Amplification Strategy for Ultrasensitive Detection of Adenosine Based on Surface Plasma Resonance Techniques
2015Co-Authors: Gui-hong Yao, Ru-ping Liang, Chun-fang Huang, Li Zhang, Jian-ding QiuAbstract:An ultrasensitive protocol for surface Plasma Resonance (SPR) detection of adenosine is designed with the aptamer-based target-triggering cascade multiple cycle amplification, and streptavidin-coated Au-NPs (Au NPs–SA) enhancement to enhance the SPR signals. The cascade amplification process consists of the aptamer-based target-triggering nicking enzyme signaling amplification (T-NESA), the nicking enzyme signaling amplification (NESA) and the hybridization chain reaction (HCR), the entire circle amplification process is triggered by the target recognition of adenosine. Upon recognition of the aptamer to target adenosine, DNA s1 is released from the aptamer and then hybridizes with hairpin DNA (HP1). The DNA s1 can be dissociated from HP1 under the reaction of nicking endonuclease to initiate the next hybridization and cleavage process. Moreover, the products of the upstream cycle (T-NESA) (DNA s2 and s3) could act as the “DNA trigger” of the downstream cycle (NESA and HCR) to generate further signal amplification, resulting in the immobilization of abundant Au NPs−SA on the gold substrate, and thus significant SPR enhancement is achieved due to the electronic coupling interaction between the localized surface Plasma of Au NPs and the surface Plasma wave. This detection method exhibits excellent specificity and sensitivity toward adenosine with a detection limit of 4 fM. The high sensitivity and specificity make this method a great potential for detecting biomolecules with trace amounts in bioanalysis and clinical biomedicine
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Target-Triggering Multiple-Cycle Amplification Strategy for Ultrasensitive Detection of Adenosine Based on Surface Plasma Resonance Techniques
Analytical chemistry, 2014Co-Authors: Gui-hong Yao, Ru-ping Liang, Chun-fang Huang, Li Zhang, Jian-ding QiuAbstract:An ultrasensitive protocol for surface Plasma Resonance (SPR) detection of adenosine is designed with the aptamer-based target-triggering cascade multiple cycle amplification, and streptavidin-coated Au-NPs (Au NPs–SA) enhancement to enhance the SPR signals. The cascade amplification process consists of the aptamer-based target-triggering nicking enzyme signaling amplification (T-NESA), the nicking enzyme signaling amplification (NESA) and the hybridization chain reaction (HCR), the entire circle amplification process is triggered by the target recognition of adenosine. Upon recognition of the aptamer to target adenosine, DNA s1 is released from the aptamer and then hybridizes with hairpin DNA (HP1). The DNA s1 can be dissociated from HP1 under the reaction of nicking endonuclease to initiate the next hybridization and cleavage process. Moreover, the products of the upstream cycle (T-NESA) (DNA s2 and s3) could act as the “DNA trigger” of the downstream cycle (NESA and HCR) to generate further signal ampl...
V B Gildenburg - One of the best experts on this subject based on the ideXlab platform.
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volume nanograting formation in laser silica interaction as a result of the 1d Plasma Resonance ionization instability
Physics of Plasmas, 2016Co-Authors: V B Gildenburg, I A PavlichenkoAbstract:The initial stage of the small-scale ionization-induced instability developing inside the fused silica volume exposed to the femtosecond laser pulse is studied as a possible initial cause of the self-organized nanograting formation. We have calculated the spatial spectra of the instability with the electron-hole diffusion taken into account for the first time and have found that it results in the formation of some hybrid (diffusion-wave) 1D structure with the spatial period determined as the geometrical mean of the laser wavelength and characteristic diffusion length of the process considered. Near the threshold of the instability, this period occurs to be approximately equal to the laser half-wavelength in the silica, close to the one experimentally observed.
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volume nanograting formation in laser silica interaction as a result of the 1d Plasma Resonance ionization instability
arXiv: Plasma Physics, 2016Co-Authors: V B Gildenburg, I A PavlichenkoAbstract:The initial stage of the small-scale ionization-induced instability developing inside the fused silica volume exposed to the femtosecond laser pulse is studied as a possible initial cause of the self-organized nanograting formation. We have calculated the spatial spectra of the instability with the electron-hole diffusion taken into account for the first time and have found that it results in the formation of some hybrid (diffusion-wave) 1D structure with the spatial period determined as geometrical mean of the laser wavelength and characteristic diffusion length of the process considered. Near the threshold of the instability this period occurs to be approximately equal to the laser half-wavelength in the silica, close to the one experimentally observed.
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steady state modification of the electron density profile in the Resonance region of moving Plasma subjected to an alternating electric field
Physics of Plasmas, 2014Co-Authors: V B Gildenburg, I A Pavlichenko, A I SimatovAbstract:Self-consistent electron density profiles of the Plasma stream interacting with the electric field of an ultrashort laser pulse are calculated in the framework of the one-dimensional steady-state model with nonlocal polarizability. These profiles are settled down against the background of a given (linear) ion density distribution as a result of the equilibrium between the averaged ponderomotive force, the static space-charge field, and the electron pressure. The average electron density and the longitudinal (parallel to the density gradient) ac electric field are found to be greatly space-modulated in the region downstream of the Plasma Resonance point.
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frequency upshifting of microwave radiation via resonant excitation of Plasma oscillations in a thin layer of a time varying Plasma
Physics of Plasmas, 2001Co-Authors: M I Bakunov, V B Gildenburg, Yasushi Nishida, Noboru YugamiAbstract:Interaction of a p-polarized microwave radiation with a thin (compared to the wavelength) Plasma layer, whose density slowly (compared to the wave period) grows in time due to ionization by an external source, e.g., laser pulse, is considered. Unlike previous papers on the frequency upshifting of electromagnetic radiation in a time-varying Plasma, which treated mainly the cases of unbounded Plasma or thick Plasma slab, a novel mechanism of frequency upshifting is presented. It is connected with the excitation of free oscillations in the Plasma layer at the moment when the Plasma density passes the critical value and, thus, Plasma Resonance in the layer occurs. The frequency of the excited Plasma oscillations increases in time following adiabatically the growing Plasma frequency, whereas the oscillations themselves gradually radiate. Due to this mechanism, the upshifted electromagnetic radiation with the frequency equal to the maximum value of the Plasma frequency in the layer can be effectively generated....
Gui-hong Yao - One of the best experts on this subject based on the ideXlab platform.
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Target-Triggering Multiple-Cycle Amplification Strategy for Ultrasensitive Detection of Adenosine Based on Surface Plasma Resonance Techniques
2015Co-Authors: Gui-hong Yao, Ru-ping Liang, Chun-fang Huang, Li Zhang, Jian-ding QiuAbstract:An ultrasensitive protocol for surface Plasma Resonance (SPR) detection of adenosine is designed with the aptamer-based target-triggering cascade multiple cycle amplification, and streptavidin-coated Au-NPs (Au NPs–SA) enhancement to enhance the SPR signals. The cascade amplification process consists of the aptamer-based target-triggering nicking enzyme signaling amplification (T-NESA), the nicking enzyme signaling amplification (NESA) and the hybridization chain reaction (HCR), the entire circle amplification process is triggered by the target recognition of adenosine. Upon recognition of the aptamer to target adenosine, DNA s1 is released from the aptamer and then hybridizes with hairpin DNA (HP1). The DNA s1 can be dissociated from HP1 under the reaction of nicking endonuclease to initiate the next hybridization and cleavage process. Moreover, the products of the upstream cycle (T-NESA) (DNA s2 and s3) could act as the “DNA trigger” of the downstream cycle (NESA and HCR) to generate further signal amplification, resulting in the immobilization of abundant Au NPs−SA on the gold substrate, and thus significant SPR enhancement is achieved due to the electronic coupling interaction between the localized surface Plasma of Au NPs and the surface Plasma wave. This detection method exhibits excellent specificity and sensitivity toward adenosine with a detection limit of 4 fM. The high sensitivity and specificity make this method a great potential for detecting biomolecules with trace amounts in bioanalysis and clinical biomedicine
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Target-Triggering Multiple-Cycle Amplification Strategy for Ultrasensitive Detection of Adenosine Based on Surface Plasma Resonance Techniques
Analytical chemistry, 2014Co-Authors: Gui-hong Yao, Ru-ping Liang, Chun-fang Huang, Li Zhang, Jian-ding QiuAbstract:An ultrasensitive protocol for surface Plasma Resonance (SPR) detection of adenosine is designed with the aptamer-based target-triggering cascade multiple cycle amplification, and streptavidin-coated Au-NPs (Au NPs–SA) enhancement to enhance the SPR signals. The cascade amplification process consists of the aptamer-based target-triggering nicking enzyme signaling amplification (T-NESA), the nicking enzyme signaling amplification (NESA) and the hybridization chain reaction (HCR), the entire circle amplification process is triggered by the target recognition of adenosine. Upon recognition of the aptamer to target adenosine, DNA s1 is released from the aptamer and then hybridizes with hairpin DNA (HP1). The DNA s1 can be dissociated from HP1 under the reaction of nicking endonuclease to initiate the next hybridization and cleavage process. Moreover, the products of the upstream cycle (T-NESA) (DNA s2 and s3) could act as the “DNA trigger” of the downstream cycle (NESA and HCR) to generate further signal ampl...
Takahiro Obara - One of the best experts on this subject based on the ideXlab platform.
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frequency dependence of polarization of zebra pattern in type iv solar radio bursts
arXiv: Solar and Stellar Astrophysics, 2015Co-Authors: Kazutaka Kaneda, Kazumasa Iwai, Fuminori Tsuchiya, Hiroaki Misawa, Takahiro ObaraAbstract:We investigated the polarization characteristics of a zebra pattern (ZP) in a type-IV solar radio burst observed with AMATERAS on 2011 June 21 for the purpose of evaluating the generation processes of ZP. Analyzing highly resolved spectral and polarization data revealed the frequency dependence of the degree of circular polarization and the delay between two polarized components for the first time. The degree of circular polarization was 50-70 percent right-handed and it varied little as a function of frequency. Cross-correlation analysis determined that the left-handed circularly polarized component was delayed by 50-70 ms relative to the right-handed component over the entire frequency range of the ZP and this delay increased with the frequency. We examined the obtained polarization characteristics by using pre-existing ZP models and concluded that the ZP was generated by the double Plasma Resonance process. Our results suggest that the ZP emission was originally generated in a completely polarized state in the O-mode and was partly converted into the X-mode near the source. Subsequently, the difference between the group velocities of the O-mode and X-mode caused the temporal delay.
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frequency dependence of polarization of zebra pattern in type iv solar radio bursts
The Astrophysical Journal, 2015Co-Authors: Kazutaka Kaneda, Kazumasa Iwai, Fuminori Tsuchiya, Hiroaki Misawa, Takahiro ObaraAbstract:We investigated the polarization characteristics of a zebra pattern (ZP) in a type-IV solar radio burst observed with AMATERAS on 2011 June 21 for the purpose of evaluating the generation processes of ZPs. Analyzing highly resolved spectral and polarization data revealed the frequency dependence of the degree of circular polarization and the delay between two polarized components for the first time. The degree of circular polarization was 50%–70% right-handed and it varied little as a function of frequency. Cross-correlation analysis determined that the left-handed circularly polarized component was delayed by 50–70 ms relative to the right-handed component over the entire frequency range of the ZP and this delay increased with the frequency. We examined the obtained polarization characteristics by using pre-existing ZP models and concluded that the ZP was generated by the double-Plasma-Resonance process. Our results suggest that the ZP emission was originally generated in a completely polarized state in the O-mode and was partly converted into the X-mode near the source. Subsequently, the difference between the group velocities of the O-mode and X-mode caused the temporal delay.
Yu T Tsap - One of the best experts on this subject based on the ideXlab platform.
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loss cone instability and formation of zebra patterns in type iv solar radio bursts
Solar Physics, 2007Co-Authors: A A Kuznetsov, Yu T TsapAbstract:The loss-cone instability of energetic electrons at double Plasma Resonance is considered. Conditions required for the formation of a zebra pattern in type IV solar radio bursts are determined. It is shown that electrons with a power-law energetic spectrum can effectively excite upper-hybrid waves at double Plasma Resonance. Stripes of a zebra pattern become more pronounced with an increase of the loss-cone opening angle and the power-law spectral index. The growth rate at the Resonance frequencies decreases with an increase of the cyclotron harmonic number. Interpretation of observations and diagnostics of Plasma for the April 21, 2002, event are performed. Conclusions about the impulsive mode of injection of energetic electrons into a coronal arc are made.