The Experts below are selected from a list of 105 Experts worldwide ranked by ideXlab platform
Colin D. Melia - One of the best experts on this subject based on the ideXlab platform.
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Determining the molar mass of a Plasma Substitute succinylated gelatin by size exclusion chromatography-multi-angle laser light scattering, sedimentation equilibrium and conventional size exclusion chromatography.
Journal of Chromatography A, 2002Co-Authors: Manjit Kaur, Kim R. Hardie, Andrea Hardman, John Meadows, Kornelia Jumel, Colin D. MeliaAbstract:Abstract The clinical effectiveness of succinylated gelatin as a Plasma Substitute depends strongly on its molar mass, determined conventionally by size exclusion chromatography (SEC). This study evaluates different SEC calibration standards in comparison with two independent “absolute” methods for determining the weight average molar mass ( M w ) of a succinylated gelatin sample. SEC calibrated using succinylated gelatin fractions correlated well with size exclusion chromatography–multi-angle laser light scattering (SEC–MALLS) and sedimentation equilibrium whereas SEC calibrated with unmodified gelatin, sodium polystyrene sulfonates or pullulans overestimated M w by over 20%. Universal calibration was equivocal. The problems associated with the preparation of succinylated gelatin fractions suggest that an absolute method such as SEC–MALLS may be a more suitable choice for determining the M w in succinylated gelatins.
Manjit Kaur - One of the best experts on this subject based on the ideXlab platform.
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Determining the molar mass of a Plasma Substitute succinylated gelatin by size exclusion chromatography-multi-angle laser light scattering, sedimentation equilibrium and conventional size exclusion chromatography.
Journal of Chromatography A, 2002Co-Authors: Manjit Kaur, Kim R. Hardie, Andrea Hardman, John Meadows, Kornelia Jumel, Colin D. MeliaAbstract:Abstract The clinical effectiveness of succinylated gelatin as a Plasma Substitute depends strongly on its molar mass, determined conventionally by size exclusion chromatography (SEC). This study evaluates different SEC calibration standards in comparison with two independent “absolute” methods for determining the weight average molar mass ( M w ) of a succinylated gelatin sample. SEC calibrated using succinylated gelatin fractions correlated well with size exclusion chromatography–multi-angle laser light scattering (SEC–MALLS) and sedimentation equilibrium whereas SEC calibrated with unmodified gelatin, sodium polystyrene sulfonates or pullulans overestimated M w by over 20%. Universal calibration was equivocal. The problems associated with the preparation of succinylated gelatin fractions suggest that an absolute method such as SEC–MALLS may be a more suitable choice for determining the M w in succinylated gelatins.
Kornelia Jumel - One of the best experts on this subject based on the ideXlab platform.
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Determining the molar mass of a Plasma Substitute succinylated gelatin by size exclusion chromatography-multi-angle laser light scattering, sedimentation equilibrium and conventional size exclusion chromatography.
Journal of Chromatography A, 2002Co-Authors: Manjit Kaur, Kim R. Hardie, Andrea Hardman, John Meadows, Kornelia Jumel, Colin D. MeliaAbstract:Abstract The clinical effectiveness of succinylated gelatin as a Plasma Substitute depends strongly on its molar mass, determined conventionally by size exclusion chromatography (SEC). This study evaluates different SEC calibration standards in comparison with two independent “absolute” methods for determining the weight average molar mass ( M w ) of a succinylated gelatin sample. SEC calibrated using succinylated gelatin fractions correlated well with size exclusion chromatography–multi-angle laser light scattering (SEC–MALLS) and sedimentation equilibrium whereas SEC calibrated with unmodified gelatin, sodium polystyrene sulfonates or pullulans overestimated M w by over 20%. Universal calibration was equivocal. The problems associated with the preparation of succinylated gelatin fractions suggest that an absolute method such as SEC–MALLS may be a more suitable choice for determining the M w in succinylated gelatins.
Kim R. Hardie - One of the best experts on this subject based on the ideXlab platform.
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Determining the molar mass of a Plasma Substitute succinylated gelatin by size exclusion chromatography-multi-angle laser light scattering, sedimentation equilibrium and conventional size exclusion chromatography.
Journal of Chromatography A, 2002Co-Authors: Manjit Kaur, Kim R. Hardie, Andrea Hardman, John Meadows, Kornelia Jumel, Colin D. MeliaAbstract:Abstract The clinical effectiveness of succinylated gelatin as a Plasma Substitute depends strongly on its molar mass, determined conventionally by size exclusion chromatography (SEC). This study evaluates different SEC calibration standards in comparison with two independent “absolute” methods for determining the weight average molar mass ( M w ) of a succinylated gelatin sample. SEC calibrated using succinylated gelatin fractions correlated well with size exclusion chromatography–multi-angle laser light scattering (SEC–MALLS) and sedimentation equilibrium whereas SEC calibrated with unmodified gelatin, sodium polystyrene sulfonates or pullulans overestimated M w by over 20%. Universal calibration was equivocal. The problems associated with the preparation of succinylated gelatin fractions suggest that an absolute method such as SEC–MALLS may be a more suitable choice for determining the M w in succinylated gelatins.
Andrea Hardman - One of the best experts on this subject based on the ideXlab platform.
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Determining the molar mass of a Plasma Substitute succinylated gelatin by size exclusion chromatography-multi-angle laser light scattering, sedimentation equilibrium and conventional size exclusion chromatography.
Journal of Chromatography A, 2002Co-Authors: Manjit Kaur, Kim R. Hardie, Andrea Hardman, John Meadows, Kornelia Jumel, Colin D. MeliaAbstract:Abstract The clinical effectiveness of succinylated gelatin as a Plasma Substitute depends strongly on its molar mass, determined conventionally by size exclusion chromatography (SEC). This study evaluates different SEC calibration standards in comparison with two independent “absolute” methods for determining the weight average molar mass ( M w ) of a succinylated gelatin sample. SEC calibrated using succinylated gelatin fractions correlated well with size exclusion chromatography–multi-angle laser light scattering (SEC–MALLS) and sedimentation equilibrium whereas SEC calibrated with unmodified gelatin, sodium polystyrene sulfonates or pullulans overestimated M w by over 20%. Universal calibration was equivocal. The problems associated with the preparation of succinylated gelatin fractions suggest that an absolute method such as SEC–MALLS may be a more suitable choice for determining the M w in succinylated gelatins.