The Experts below are selected from a list of 44133 Experts worldwide ranked by ideXlab platform

Akihiko Yamagishi - One of the best experts on this subject based on the ideXlab platform.

  • An efficient gene replacement and deletion system for an extreme thermophile, Thermus thermophilus.
    Fems Microbiology Letters, 1999
    Co-Authors: Masatada Tamakoshi, Takuro Yaoi, Tairo Oshima, Akihiko Yamagishi
    Abstract:

    A Thermus thermophilus host strain of which the leuB gene was totally deleted was constructed from a ΔpyrE strain by a two step method. First, the leuB gene was replaced with the pyrE gene. Second, the inserted pyrE gene was deleted by using 5-fluoroorotic acid. A Plasmid Vector with the leuB marker was constructed and the Plasmid complemented the leuB deficiency of the host. When the leuB gene from Escherichia coli and its derivative encoding a stabilized enzyme were expressed with the host-Vector system, their growth temperature reflected the stability of the enzyme. These results suggest that the gene replacement deletion method using the pyrE gene is useful for the construction of a reliable Plasmid Vector system and it can be applied to the selection of stabilized enzymes.

Masaki Iwai - One of the best experts on this subject based on the ideXlab platform.

  • Polyethylenimine-Mediated Suicide Gene Transfer Induces a Therapeutic Effect for Hepatocellular Carcinoma in Vivo by Using an Epstein–Barr Virus-Based Plasmid Vector
    Biochemical and biophysical research communications, 2002
    Co-Authors: Masaki Iwai, Yoshinori Harada, Jiro Imanishi, Saiyu Tanaka, Akira Muramatsu, Takahiro Mori, Kei Kashima, Osam Mazda
    Abstract:

    The present study aimed to establish a novel efficient nonviral strategy for suicide gene transfer in hepatocellular carcinoma (HCC) in vivo. We employed branched polyethylenimine (PEI) and combined it with Epstein-Barr virus (EBV)-based Plasmid Vectors. The HCC cells transfected with an EBV-based Plasmid carrying the herpes simplex virus-1 thymidine kinase (HSV-1 Tk) gene (pSES.Tk) showed up to 30-fold higher susceptibilities to ganciclovir (GCV) than those transfected with a conventional Plasmid Vector carrying the HSV-1 Tk gene (pS.Tk). The therapeutic effect in vivo was tested by intratumoral injection of the Plasmids into HuH-7 hepatomas transplanted into C.B-17 scid/scid mutant (SCID) mice and subsequent GCV administrations. Treatment with pSES.Tk, but not pS.Tk, markedly suppressed growth of hepatomas in vivo, resulting in a significantly prolonged survival period of the mice. These findings suggest that PEI-mediated gene transfer system can confer efficient expression of the suicide gene in HCC cells in vivo by using EBV-based Plasmid Vectors.

  • Effective suicide gene therapy in vivo by EBV-based Plasmid Vector coupled with polyamidoamine dendrimer.
    Gene therapy, 2000
    Co-Authors: Hiroko Maruyama-tabata, Yoshinori Harada, Takafumi Matsumura, Etsuko Satoh, Feng-de Cui, Masaki Iwai, Masakazu Kita, Shigeyoshi Hibi, Jiro Imanishi, Tadashi Sawada
    Abstract:

    This study demonstrates in vivo effectiveness of a nonviral Vector system, Epstein–Barr virus (EBV)-based Plasmid Vector coupled with polyamidoamine (PAMAM) dendrimer (EBV/polyplex), in suicide gene therapy of cancer. The EBV-based Vector is a Plasmid Vector containing EBV nuclear antigen 1 (EBNA1) gene and oriP from EBV genome. HSV-1 tk gene was transferred into Ewing's sarcoma cell lines, A4573 and KP-EWS-YI, by using an EBV-based Plasmid Vector, pSES.Tk, or a conventional Plasmid Vector, pS.Tk. Cells transfected with pSES.Tk/dendrimer showed approximately 100 times lower ID50 to ganciclovir (GCV) compared with those transfected with pS.Tk/dendrimer. Intratumoral injection of pSES.Tk/dendrimer but not pS.Tk/dendrimer drastically suppressed the growth of tumors which had generated from A4573 or Huh7 hepatocellular carcinoma (HCC) cells inoculated into severe combined immunodeficiency (SCID) mice. The treatment with pSES.Tk/dendrimer also resulted in significant prolongation of survival of the mice implanted with A4573. These results suggest that the EBV/polyplex system could be useful for in vivo suicide gene therapy of cancer.

  • NONVIRAL TRANSFER TECHNIQUES RESEARCH ARTICLE Effective suicide gene therapy in vivo by EBV-based Plasmid Vector coupled with polyamidoamine dendrimer
    2000
    Co-Authors: Hiroko Maruyama-tabata, Yoshinori Harada, Takafumi Matsumura, Etsuko Satoh, Masaki Iwai, Masakazu Kita, Shigeyoshi Hibi, Jiro Imanishi, F Cui, Tadashi Sawada
    Abstract:

    This study demonstrates in vivo effectiveness of a nonviral Vector system, Epstein‐Barr virus (EBV)-based Plasmid Vector coupled with polyamidoamine (PAMAM) dendrimer (EBV/polyplex), in suicide gene therapy of cancer. The EBVbased Vector is a Plasmid Vector containing EBV nuclear antigen 1 (EBNA1) gene and oriP from EBV genome. HSV1 tk gene was transferred into Ewing’s sarcoma cell lines, A4573 and KP-EWS-YI, by using an EBV-based Plasmid Vector, pSES.Tk, or a conventional Plasmid Vector, pS.Tk. Cells transfected with pSES.Tk/dendrimer showed approximately 100 times lower ID50 to ganciclovir (GCV) compared

Masatada Tamakoshi - One of the best experts on this subject based on the ideXlab platform.

  • An efficient gene replacement and deletion system for an extreme thermophile, Thermus thermophilus.
    Fems Microbiology Letters, 1999
    Co-Authors: Masatada Tamakoshi, Takuro Yaoi, Tairo Oshima, Akihiko Yamagishi
    Abstract:

    A Thermus thermophilus host strain of which the leuB gene was totally deleted was constructed from a ΔpyrE strain by a two step method. First, the leuB gene was replaced with the pyrE gene. Second, the inserted pyrE gene was deleted by using 5-fluoroorotic acid. A Plasmid Vector with the leuB marker was constructed and the Plasmid complemented the leuB deficiency of the host. When the leuB gene from Escherichia coli and its derivative encoding a stabilized enzyme were expressed with the host-Vector system, their growth temperature reflected the stability of the enzyme. These results suggest that the gene replacement deletion method using the pyrE gene is useful for the construction of a reliable Plasmid Vector system and it can be applied to the selection of stabilized enzymes.

Tadashi Sawada - One of the best experts on this subject based on the ideXlab platform.

  • Effective suicide gene therapy in vivo by EBV-based Plasmid Vector coupled with polyamidoamine dendrimer.
    Gene therapy, 2000
    Co-Authors: Hiroko Maruyama-tabata, Yoshinori Harada, Takafumi Matsumura, Etsuko Satoh, Feng-de Cui, Masaki Iwai, Masakazu Kita, Shigeyoshi Hibi, Jiro Imanishi, Tadashi Sawada
    Abstract:

    This study demonstrates in vivo effectiveness of a nonviral Vector system, Epstein–Barr virus (EBV)-based Plasmid Vector coupled with polyamidoamine (PAMAM) dendrimer (EBV/polyplex), in suicide gene therapy of cancer. The EBV-based Vector is a Plasmid Vector containing EBV nuclear antigen 1 (EBNA1) gene and oriP from EBV genome. HSV-1 tk gene was transferred into Ewing's sarcoma cell lines, A4573 and KP-EWS-YI, by using an EBV-based Plasmid Vector, pSES.Tk, or a conventional Plasmid Vector, pS.Tk. Cells transfected with pSES.Tk/dendrimer showed approximately 100 times lower ID50 to ganciclovir (GCV) compared with those transfected with pS.Tk/dendrimer. Intratumoral injection of pSES.Tk/dendrimer but not pS.Tk/dendrimer drastically suppressed the growth of tumors which had generated from A4573 or Huh7 hepatocellular carcinoma (HCC) cells inoculated into severe combined immunodeficiency (SCID) mice. The treatment with pSES.Tk/dendrimer also resulted in significant prolongation of survival of the mice implanted with A4573. These results suggest that the EBV/polyplex system could be useful for in vivo suicide gene therapy of cancer.

  • NONVIRAL TRANSFER TECHNIQUES RESEARCH ARTICLE Effective suicide gene therapy in vivo by EBV-based Plasmid Vector coupled with polyamidoamine dendrimer
    2000
    Co-Authors: Hiroko Maruyama-tabata, Yoshinori Harada, Takafumi Matsumura, Etsuko Satoh, Masaki Iwai, Masakazu Kita, Shigeyoshi Hibi, Jiro Imanishi, F Cui, Tadashi Sawada
    Abstract:

    This study demonstrates in vivo effectiveness of a nonviral Vector system, Epstein‐Barr virus (EBV)-based Plasmid Vector coupled with polyamidoamine (PAMAM) dendrimer (EBV/polyplex), in suicide gene therapy of cancer. The EBVbased Vector is a Plasmid Vector containing EBV nuclear antigen 1 (EBNA1) gene and oriP from EBV genome. HSV1 tk gene was transferred into Ewing’s sarcoma cell lines, A4573 and KP-EWS-YI, by using an EBV-based Plasmid Vector, pSES.Tk, or a conventional Plasmid Vector, pS.Tk. Cells transfected with pSES.Tk/dendrimer showed approximately 100 times lower ID50 to ganciclovir (GCV) compared

Yoshinori Harada - One of the best experts on this subject based on the ideXlab platform.

  • Polyethylenimine-Mediated Suicide Gene Transfer Induces a Therapeutic Effect for Hepatocellular Carcinoma in Vivo by Using an Epstein–Barr Virus-Based Plasmid Vector
    Biochemical and biophysical research communications, 2002
    Co-Authors: Masaki Iwai, Yoshinori Harada, Jiro Imanishi, Saiyu Tanaka, Akira Muramatsu, Takahiro Mori, Kei Kashima, Osam Mazda
    Abstract:

    The present study aimed to establish a novel efficient nonviral strategy for suicide gene transfer in hepatocellular carcinoma (HCC) in vivo. We employed branched polyethylenimine (PEI) and combined it with Epstein-Barr virus (EBV)-based Plasmid Vectors. The HCC cells transfected with an EBV-based Plasmid carrying the herpes simplex virus-1 thymidine kinase (HSV-1 Tk) gene (pSES.Tk) showed up to 30-fold higher susceptibilities to ganciclovir (GCV) than those transfected with a conventional Plasmid Vector carrying the HSV-1 Tk gene (pS.Tk). The therapeutic effect in vivo was tested by intratumoral injection of the Plasmids into HuH-7 hepatomas transplanted into C.B-17 scid/scid mutant (SCID) mice and subsequent GCV administrations. Treatment with pSES.Tk, but not pS.Tk, markedly suppressed growth of hepatomas in vivo, resulting in a significantly prolonged survival period of the mice. These findings suggest that PEI-mediated gene transfer system can confer efficient expression of the suicide gene in HCC cells in vivo by using EBV-based Plasmid Vectors.

  • Effective suicide gene therapy in vivo by EBV-based Plasmid Vector coupled with polyamidoamine dendrimer.
    Gene therapy, 2000
    Co-Authors: Hiroko Maruyama-tabata, Yoshinori Harada, Takafumi Matsumura, Etsuko Satoh, Feng-de Cui, Masaki Iwai, Masakazu Kita, Shigeyoshi Hibi, Jiro Imanishi, Tadashi Sawada
    Abstract:

    This study demonstrates in vivo effectiveness of a nonviral Vector system, Epstein–Barr virus (EBV)-based Plasmid Vector coupled with polyamidoamine (PAMAM) dendrimer (EBV/polyplex), in suicide gene therapy of cancer. The EBV-based Vector is a Plasmid Vector containing EBV nuclear antigen 1 (EBNA1) gene and oriP from EBV genome. HSV-1 tk gene was transferred into Ewing's sarcoma cell lines, A4573 and KP-EWS-YI, by using an EBV-based Plasmid Vector, pSES.Tk, or a conventional Plasmid Vector, pS.Tk. Cells transfected with pSES.Tk/dendrimer showed approximately 100 times lower ID50 to ganciclovir (GCV) compared with those transfected with pS.Tk/dendrimer. Intratumoral injection of pSES.Tk/dendrimer but not pS.Tk/dendrimer drastically suppressed the growth of tumors which had generated from A4573 or Huh7 hepatocellular carcinoma (HCC) cells inoculated into severe combined immunodeficiency (SCID) mice. The treatment with pSES.Tk/dendrimer also resulted in significant prolongation of survival of the mice implanted with A4573. These results suggest that the EBV/polyplex system could be useful for in vivo suicide gene therapy of cancer.

  • NONVIRAL TRANSFER TECHNIQUES RESEARCH ARTICLE Effective suicide gene therapy in vivo by EBV-based Plasmid Vector coupled with polyamidoamine dendrimer
    2000
    Co-Authors: Hiroko Maruyama-tabata, Yoshinori Harada, Takafumi Matsumura, Etsuko Satoh, Masaki Iwai, Masakazu Kita, Shigeyoshi Hibi, Jiro Imanishi, F Cui, Tadashi Sawada
    Abstract:

    This study demonstrates in vivo effectiveness of a nonviral Vector system, Epstein‐Barr virus (EBV)-based Plasmid Vector coupled with polyamidoamine (PAMAM) dendrimer (EBV/polyplex), in suicide gene therapy of cancer. The EBVbased Vector is a Plasmid Vector containing EBV nuclear antigen 1 (EBNA1) gene and oriP from EBV genome. HSV1 tk gene was transferred into Ewing’s sarcoma cell lines, A4573 and KP-EWS-YI, by using an EBV-based Plasmid Vector, pSES.Tk, or a conventional Plasmid Vector, pS.Tk. Cells transfected with pSES.Tk/dendrimer showed approximately 100 times lower ID50 to ganciclovir (GCV) compared