The Experts below are selected from a list of 303 Experts worldwide ranked by ideXlab platform

Chea Nguon - One of the best experts on this subject based on the ideXlab platform.

Yaping Gu - One of the best experts on this subject based on the ideXlab platform.

Alassane Mbengue - One of the best experts on this subject based on the ideXlab platform.

  • a molecular mechanism of artemisinin resistance in Plasmodium falciparum malaria
    Nature, 2015
    Co-Authors: Alassane Mbengue, Souvik Bhattacharjee, Trupti Pandharkar, Shahir S Rizk, Dieudonne Lemuh Njimoh, Yana Ryan, Kesinee Chotivanich, Robert V Stahelin, Guillermina Estiu, Chea Nguon
    Abstract:

    Artemisinins are key anti-malarial drugs, but artemisinin resistance has been increasing; this study identifies the molecular target of artemisinins as phosphatidylinositol-3-kinase and increase of the lipid product phosphatidylinositol-3-phosphate induces resistance in Plasmodium falciparum.

  • Human Erythrocyte Remodelling by Plasmodium falciparum
    Malaria Parasites, 2012
    Co-Authors: Alassane Mbengue, Catherine Braun-breton
    Abstract:

    Making the erythrocyte its home for 48 hours has important consequences for the human malaria parasite Plasmodium falciparum. Indeed, erythrocytes are terminally differentiated cells that lack a nucleus as well as intracellular organelles, are thus unable to endocytose or exocytose macromolecules, and have lost several membrane transport activities upon differentiation. Consequently, the parasite has to deeply remodel its host cell, from the very beginning to ensure its entry into the red blood cell, throughout its growth and multiplication to fulfil its needs for extracellular nutrients, and to the very end of its intraerythrocytic development with the parasite-induced opening and curling of the red cell membrane leading to the dispersion of newly formed merozoites into the blood flow. The most spectacular, and first reported, modifications of the red blood cell membrane induced by P. falciparum are electron dense protrusions named knobs and consisting of parasite proteins exported to the red cell membrane and sub-membrane skeleton where they eventually interact with host cell proteins. Knobs are directly related to the severity of falciparum malaria because they mediate adherence of infected erythrocytes to the microvasculature endothelium. More recent studies have revealed that the parasite might export several hundreds of proteins, as well as membrane compartments, to the red cell and divert enzymatic and structural host proteins to make the erythrocyte a suitable environment for its growth. In the last decade, remodelling of its host cell by Plasmodium falciparum has become an important and growing field of research. In this review, we will describe the current stage of knowledge concerning red blood cell remodelling by Plasmodium falciparum and the role of these parasite-induced modifications for its growth and survival.

Feng Lu - One of the best experts on this subject based on the ideXlab platform.

Yan Yb - One of the best experts on this subject based on the ideXlab platform.

  • Molecular recognition between GlcNAc and Plasmodium falciparum merozoites
    Chinese Journal of Parasitology and Parasitic Diseases, 1994
    Co-Authors: Zhu K, Li Jx, Wei Ym, Zheng Z, Chen Km, Yan Yb
    Abstract:

    : In this study p-aminophenyl-2-acetamido-2-deoxy-beta-D-glucopyranoside was bound covalently to bovine serum albumin (BSA) to form neoglycoprotein (GlcNAc-BSA). The antigenicity of BSA and the pyranose structure of the sugar was preserved. By using the neoglycoprotein antibody against BSA and staphylococcal protein A-gold (SPA-gold), Plasmodium falciparum FCC-1/HN merozoites were immunolabeled. The labelled sample was observed under transmission electron microscope (TEM). The TEM pictures showed that colloidal gold pellets were distributed all over the merozoite surface. This is the first report on the direct experimental evidence of molecular recognition between GlcNAc-BSA (or GlcNAc) and Plasmodium falciparum merozoites. But free GlcNAc or even GlcN can inhibit the immunolabeleation. The results support the hypothesis that, besides N-acetylneuraminic acid, GlcNAc is involved as another recognized site on GPA for Plasmodium falciparum. Thus we further confirmed that N-acetyl of GlcNAc is not necessary for the recognition.

  • Molecular recognition between glycophorin A and Plasmodium falciparum merozoites
    Chinese Journal of Parasitology and Parasitic Diseases, 1991
    Co-Authors: Wang Xd, Li Jx, Wei Ym, Zheng Z, Yan Yb
    Abstract:

    : By using purified human erythrocyte membrane glycophorin A (GPA) and glycopeptide of GPA, antibodies against GPA and against GPA-glycopeptide, and SPA-colloidal gold, Plasmodium falciparum FCC-1/HN merozoites were immunolabeled. The labeled samples were observed under transmission electron microscope (TEM). The TEM pictures showed that colloidal gold pellets were distributed over all of the merozoite surface. This is the first report on the direct experimental evidence of molecular recognition and combination between GPA (or glycopeptide of GPA) and Plasmodium falciparum merozoites. The results strongly support the hypothesis that GPA is involved as a recognized receptor on erythrocytes for Plasmodium falciparum and the glycopeptide domain of GPA is the receptor site for Plasmodium falciparum.