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J B Mcalpine - One of the best experts on this subject based on the ideXlab platform.

  • A method for the selective isolation ofMyxococcus directly from soil
    Journal of Industrial Microbiology, 1996
    Co-Authors: J P Karwowski, G N Sunga, S Kadam, J B Mcalpine
    Abstract:

    A new method is described for the selective isolation of species of Myxococcus directly from soil by dilution Plating. The method involves suppression of competing microorganisms with antibiotics combined with air drying and wet heat treatment of soils. Fungi were eliminated by supplementing the Plating Medium with cycloheximide and nystatin. Non-sporulating bacteria were controlled by air drying soils and then heating aqueous soil dilutions for 10 min at 56°C. The predominant sporulating bacteria in soil, Streptomyces and Bacillus , were suppressed by adding either tiacumicin B, ristocetin or vancomycin to the Medium. Swarming of Myxococcus colonies was controlled with a casein digest-yeast extract Plating Medium (CY-C10 agar). Ultrasound treatment of soil suspensions gave the highest number of Myxococcus colonies in the soils studied, but these cultures could be recovered without ultrasound. Strains of Myxococccus fulvus, M. xanthus, M. coralloides, M. stipitatus and M. virescens were isolated from soil using this technique. Soils examined yielded one or two Myxococcus species per sample.

  • A method for the selective isolation of Myxococcus directly from soil
    Journal of Industrial Microbiology & Biotechnology, 1996
    Co-Authors: J P Karwowski, G N Sunga, S Kadam, J B Mcalpine
    Abstract:

    A new method is described for the selective isolation of species ofMyxococcus directly from soil by dilution Plating. The method involves suppression of competing microorganisms with antibiotics combined with air drying and wet heat treatment of soils. Fungi were eliminated by supplementing the Plating Medium with cycloheximide and nystatin. Non-sporulating bacteria were controlled by air drying soils and then heating aqueous soil dilutions for 10 min at 56°C. The predominant sporulating bacteria in soil,Streptomyces andBacillus, were suppressed by adding either tiacumicin B, ristocetin or vancomycin to the Medium. Swarming ofMyxococcus colonies was controlled with a casein digest-yeast extract Plating Medium (CY-C10 agar). Ultrasound treatment of soil suspensions gave the highest number ofMyxococcus colonies in the soils studied, but these cultures could be recovered without ultrasound. Strains ofMyxococccus fulvus, M. xanthus, M. coralloides, M. stipitatus andM. virescens were isolated from soil using this technique. Soils examined yielded one or twoMyxococcus species per sample.

J P Karwowski - One of the best experts on this subject based on the ideXlab platform.

  • A method for the selective isolation ofMyxococcus directly from soil
    Journal of Industrial Microbiology, 1996
    Co-Authors: J P Karwowski, G N Sunga, S Kadam, J B Mcalpine
    Abstract:

    A new method is described for the selective isolation of species of Myxococcus directly from soil by dilution Plating. The method involves suppression of competing microorganisms with antibiotics combined with air drying and wet heat treatment of soils. Fungi were eliminated by supplementing the Plating Medium with cycloheximide and nystatin. Non-sporulating bacteria were controlled by air drying soils and then heating aqueous soil dilutions for 10 min at 56°C. The predominant sporulating bacteria in soil, Streptomyces and Bacillus , were suppressed by adding either tiacumicin B, ristocetin or vancomycin to the Medium. Swarming of Myxococcus colonies was controlled with a casein digest-yeast extract Plating Medium (CY-C10 agar). Ultrasound treatment of soil suspensions gave the highest number of Myxococcus colonies in the soils studied, but these cultures could be recovered without ultrasound. Strains of Myxococccus fulvus, M. xanthus, M. coralloides, M. stipitatus and M. virescens were isolated from soil using this technique. Soils examined yielded one or two Myxococcus species per sample.

  • A method for the selective isolation of Myxococcus directly from soil
    Journal of Industrial Microbiology & Biotechnology, 1996
    Co-Authors: J P Karwowski, G N Sunga, S Kadam, J B Mcalpine
    Abstract:

    A new method is described for the selective isolation of species ofMyxococcus directly from soil by dilution Plating. The method involves suppression of competing microorganisms with antibiotics combined with air drying and wet heat treatment of soils. Fungi were eliminated by supplementing the Plating Medium with cycloheximide and nystatin. Non-sporulating bacteria were controlled by air drying soils and then heating aqueous soil dilutions for 10 min at 56°C. The predominant sporulating bacteria in soil,Streptomyces andBacillus, were suppressed by adding either tiacumicin B, ristocetin or vancomycin to the Medium. Swarming ofMyxococcus colonies was controlled with a casein digest-yeast extract Plating Medium (CY-C10 agar). Ultrasound treatment of soil suspensions gave the highest number ofMyxococcus colonies in the soils studied, but these cultures could be recovered without ultrasound. Strains ofMyxococccus fulvus, M. xanthus, M. coralloides, M. stipitatus andM. virescens were isolated from soil using this technique. Soils examined yielded one or twoMyxococcus species per sample.

Donald G. Macphee - One of the best experts on this subject based on the ideXlab platform.

  • Glucose inhibition of mutagenesis by 9-aminoacridine in Salmonella typhimurium
    Mutation Research, 2003
    Co-Authors: George Kopsidas, Donald G. Macphee
    Abstract:

    Abstract Back mutation to prototrophy of the hisC3076 marker of Salmonella typhimurium has been measured following treatment with 9-aminoacridine (9AA) under conditions in which growth is either permitted or not permitted. Cells treated with 9AA in buffer (i.e. under conditions in which little or no replication was possible) were found to respond well to 9AA-induced mutagenesis. This remained true whether or not the Plating Medium contained trace amounts of histidine to allow residual replication of His − cells (as for example in the Ames test); yields of His + mutants were also about the same regardless of whether the Plating Medium contained glucose as the sole carbon source. By contrast, 9AA-induced mutagenesis was essentially abolished when cells were treated in buffer containing 1% glucose (i.e. under conditions which strongly favoured replication). Glucose inhibition of 9AA-induced mutagenesis began to be apparent at glucose concentrations of about 0.02%, whilst total abolition was observed at about 0.2%. In addition, glucose inhibition was found to be dependent on the concentration of 9AA, the induced mutation rate increasing at levels of up to 100–500 μg/mol of 9AA and then declining steeply at higher levels. Further kinetic studies indicated that glucose could depress the 9AA-induced mutation rate significantly even in cells exposed to the mutagen for up to 18 min prior to the addition of glucose, whilst inhibition of 9AA mutagenesis by glucose was both temporary and reversible.

  • Precise excision of TnlO in Salmonella typhimurium: effects of mutations in the polA, dam, mutH and mutB genes and of methionine or ethionine in the Plating Medium
    Mutation Research Letters, 1991
    Co-Authors: Louise M. Hafner, Donald G. Macphee
    Abstract:

    Abstract Precise excision of Tn10 occurs at significantly elevated frequencies in cultures of the polA 7 mutant strain of Salmonella typhimurium, is further increased in polA7dam-1 and polA7mutB strains and decreased in a polA7mutH background. The numbers of precise excision events occurring in polA7 strains are also significantly increased when methionine (20 μg/ml or less) is present in the Plating Medium but decreased when ethionine (again, 20 μg/ml or less) is present. When both amino acids are present, the outcome is about a 2-fold increase in precise excision events. The involvement of mismatch repair and methylation patterns in precise excision events is discussed.

S Kadam - One of the best experts on this subject based on the ideXlab platform.

  • A method for the selective isolation ofMyxococcus directly from soil
    Journal of Industrial Microbiology, 1996
    Co-Authors: J P Karwowski, G N Sunga, S Kadam, J B Mcalpine
    Abstract:

    A new method is described for the selective isolation of species of Myxococcus directly from soil by dilution Plating. The method involves suppression of competing microorganisms with antibiotics combined with air drying and wet heat treatment of soils. Fungi were eliminated by supplementing the Plating Medium with cycloheximide and nystatin. Non-sporulating bacteria were controlled by air drying soils and then heating aqueous soil dilutions for 10 min at 56°C. The predominant sporulating bacteria in soil, Streptomyces and Bacillus , were suppressed by adding either tiacumicin B, ristocetin or vancomycin to the Medium. Swarming of Myxococcus colonies was controlled with a casein digest-yeast extract Plating Medium (CY-C10 agar). Ultrasound treatment of soil suspensions gave the highest number of Myxococcus colonies in the soils studied, but these cultures could be recovered without ultrasound. Strains of Myxococccus fulvus, M. xanthus, M. coralloides, M. stipitatus and M. virescens were isolated from soil using this technique. Soils examined yielded one or two Myxococcus species per sample.

  • A method for the selective isolation of Myxococcus directly from soil
    Journal of Industrial Microbiology & Biotechnology, 1996
    Co-Authors: J P Karwowski, G N Sunga, S Kadam, J B Mcalpine
    Abstract:

    A new method is described for the selective isolation of species ofMyxococcus directly from soil by dilution Plating. The method involves suppression of competing microorganisms with antibiotics combined with air drying and wet heat treatment of soils. Fungi were eliminated by supplementing the Plating Medium with cycloheximide and nystatin. Non-sporulating bacteria were controlled by air drying soils and then heating aqueous soil dilutions for 10 min at 56°C. The predominant sporulating bacteria in soil,Streptomyces andBacillus, were suppressed by adding either tiacumicin B, ristocetin or vancomycin to the Medium. Swarming ofMyxococcus colonies was controlled with a casein digest-yeast extract Plating Medium (CY-C10 agar). Ultrasound treatment of soil suspensions gave the highest number ofMyxococcus colonies in the soils studied, but these cultures could be recovered without ultrasound. Strains ofMyxococccus fulvus, M. xanthus, M. coralloides, M. stipitatus andM. virescens were isolated from soil using this technique. Soils examined yielded one or twoMyxococcus species per sample.

G N Sunga - One of the best experts on this subject based on the ideXlab platform.

  • A method for the selective isolation ofMyxococcus directly from soil
    Journal of Industrial Microbiology, 1996
    Co-Authors: J P Karwowski, G N Sunga, S Kadam, J B Mcalpine
    Abstract:

    A new method is described for the selective isolation of species of Myxococcus directly from soil by dilution Plating. The method involves suppression of competing microorganisms with antibiotics combined with air drying and wet heat treatment of soils. Fungi were eliminated by supplementing the Plating Medium with cycloheximide and nystatin. Non-sporulating bacteria were controlled by air drying soils and then heating aqueous soil dilutions for 10 min at 56°C. The predominant sporulating bacteria in soil, Streptomyces and Bacillus , were suppressed by adding either tiacumicin B, ristocetin or vancomycin to the Medium. Swarming of Myxococcus colonies was controlled with a casein digest-yeast extract Plating Medium (CY-C10 agar). Ultrasound treatment of soil suspensions gave the highest number of Myxococcus colonies in the soils studied, but these cultures could be recovered without ultrasound. Strains of Myxococccus fulvus, M. xanthus, M. coralloides, M. stipitatus and M. virescens were isolated from soil using this technique. Soils examined yielded one or two Myxococcus species per sample.

  • A method for the selective isolation of Myxococcus directly from soil
    Journal of Industrial Microbiology & Biotechnology, 1996
    Co-Authors: J P Karwowski, G N Sunga, S Kadam, J B Mcalpine
    Abstract:

    A new method is described for the selective isolation of species ofMyxococcus directly from soil by dilution Plating. The method involves suppression of competing microorganisms with antibiotics combined with air drying and wet heat treatment of soils. Fungi were eliminated by supplementing the Plating Medium with cycloheximide and nystatin. Non-sporulating bacteria were controlled by air drying soils and then heating aqueous soil dilutions for 10 min at 56°C. The predominant sporulating bacteria in soil,Streptomyces andBacillus, were suppressed by adding either tiacumicin B, ristocetin or vancomycin to the Medium. Swarming ofMyxococcus colonies was controlled with a casein digest-yeast extract Plating Medium (CY-C10 agar). Ultrasound treatment of soil suspensions gave the highest number ofMyxococcus colonies in the soils studied, but these cultures could be recovered without ultrasound. Strains ofMyxococccus fulvus, M. xanthus, M. coralloides, M. stipitatus andM. virescens were isolated from soil using this technique. Soils examined yielded one or twoMyxococcus species per sample.