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Jennifer K. Ketzis - One of the best experts on this subject based on the ideXlab platform.
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Comparison of fecal analysis methods for the detection of Platynosomum fastosum in naturally infected cats
Journal of feline medicine and surgery, 2019Co-Authors: Hilary Eisenbraun, Adnan Alluwie, Samantha Connolly, Rajeev Nair, Guilherme G. Verocai, Jennifer K. KetzisAbstract:ObjectivesDiagnosis of Platynosomum fastosum Kossack 1910, a liver fluke of cats, can be challenging, with infections potentially not identified depending on the fecal analysis method used. The aim of this study was to compare three methods for identification of P fastosum eggs in feces.MethodsFeces from 50 cats with unknown infection status from a location endemic for P fastosum were analyzed. The methods used included double centrifugation with Sheather’s sugar flotation solution (specific gravity 1.28); the Mini-Parasep (Apacor) flotation kit with ZnSO4 (specific gravity 1.20); and the Mini-Parasep sedimentation kit with Triton X/ethyl acetate solution.ResultsThirty-four cats were identified as positive for P fastosum. Of these cats, 33 were identified with the double centrifugation method, 16 with the Mini-Parasep flotation kit and 11 with the Mini-Parasep sedimentation kit. Using the total number of samples identified as positive by at least one method as the true positive, the sensitivity of the met...
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percutaneous ultrasound guided cholecystocentesis and bile analysis for the detection of Platynosomum spp induced cholangitis in cats
Journal of Veterinary Internal Medicine, 2016Co-Authors: Liza S Köster, Oscar Illanes, C. Lathroum, K. Neuville, Jennifer K. KetzisAbstract:Background Examination of bile could be useful to diagnose Platynosomum spp.-induced cholangitis in cats. Obtaining bile via percutaneous ultrasound-guided cholecystocentesis (PUC) is possible but raises safety concerns in cats with severe cholecystitis. Objectives The objectives of this study were to investigate the use of PUC to collect bile samples from cats with known platynosomosis and to determine if bile analysis could be a diagnostic test. Animals Twenty-seven free-roaming cats positive for Platynosomum spp. eggs via fecal examination. Methods In this prospective study, fecal egg counts were performed by double centrifugation with Sheather's solution. Bile was collected using PUC from anesthetized cats. Egg counts in bile were performed with a stereoscope. Euthanasia and postmortem examination were performed immediately after PUC. Results All cats had ultrasound (US) evidence of cholangitis or cholecystitis. Thirty-nine PUCs were performed with 14 cats having 2 PUCs 12 or 24 days apart. Postmortem examinations showed no overt gallbladder damage or leakage but fresh blood was noted in the gallbladder lumen of 3 cats. Median Platynosomum spp. egg counts were higher in bile (1450 eggs/mL; IQR, 400; 5138 eggs/mL) as compared to feces (46 eggs/mL; IQR, 10; 107 eggs/mL) (P < .001). Conclusion and Clinical Importance Bile egg count analysis is an alternative method with higher egg counts as compared to fecal egg count analysis for the diagnosis of platynosomosis. Obtaining bile via US guidance is technically feasible and safe in cats with cholangitis/cholecystitis. Cholecystocentesis and bile analysis are especially relevant for those cats with chronic cholangitis/cholecystitis and negative fecal egg counts for Platynosomum.
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Percutaneous Ultrasound‐guided Cholecystocentesis and Bile Analysis for the Detection of Platynosomum spp.‐Induced Cholangitis in Cats
Journal of veterinary internal medicine, 2016Co-Authors: Liza S Köster, Oscar Illanes, C. Lathroum, K. Neuville, Jennifer K. KetzisAbstract:Background Examination of bile could be useful to diagnose Platynosomum spp.-induced cholangitis in cats. Obtaining bile via percutaneous ultrasound-guided cholecystocentesis (PUC) is possible but raises safety concerns in cats with severe cholecystitis. Objectives The objectives of this study were to investigate the use of PUC to collect bile samples from cats with known platynosomosis and to determine if bile analysis could be a diagnostic test. Animals Twenty-seven free-roaming cats positive for Platynosomum spp. eggs via fecal examination. Methods In this prospective study, fecal egg counts were performed by double centrifugation with Sheather's solution. Bile was collected using PUC from anesthetized cats. Egg counts in bile were performed with a stereoscope. Euthanasia and postmortem examination were performed immediately after PUC. Results All cats had ultrasound (US) evidence of cholangitis or cholecystitis. Thirty-nine PUCs were performed with 14 cats having 2 PUCs 12 or 24 days apart. Postmortem examinations showed no overt gallbladder damage or leakage but fresh blood was noted in the gallbladder lumen of 3 cats. Median Platynosomum spp. egg counts were higher in bile (1450 eggs/mL; IQR, 400; 5138 eggs/mL) as compared to feces (46 eggs/mL; IQR, 10; 107 eggs/mL) (P < .001). Conclusion and Clinical Importance Bile egg count analysis is an alternative method with higher egg counts as compared to fecal egg count analysis for the diagnosis of platynosomosis. Obtaining bile via US guidance is technically feasible and safe in cats with cholangitis/cholecystitis. Cholecystocentesis and bile analysis are especially relevant for those cats with chronic cholangitis/cholecystitis and negative fecal egg counts for Platynosomum.
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Brief Original Article The prevalence of Trichuris spp. infection in indoor and outdoor cats on St.
2015Co-Authors: Jennifer K. Ketzis, Linda Shell, Sarah Chinault, Charles Pemberton, Mary Mauldin PereiraAbstract:Introduction: The present study was conducted to evaluate the prevalence of Trichuris spp. and other intestinal parasitic infections in owned cats on St. Kitts. Methodology: The feces of 41 non-feral cats (23 indoor only; 18 indoor/outdoor) were examined for the presence of Trichuris spp. eggs. Results: Nine (22%) of the cats were positive for Trichuris spp. Prevalence of trichuriasis in indoor cats was 26.0 % as compared to 16.7 % in outdoor cats. Other parasites identified included Ancylostoma spp. (10%; 4 cats), Toxocara cati (2%; 1 cat), Platynosomum spp. (22%;
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Praziquantel treatment for Platynosomum species infection of a domestic cat on St Kitts, West Indies.
JFMS open reports, 2015Co-Authors: Linda Shell, Jennifer K. Ketzis, Rebecca Hall, Gilda Rawlins, Wencke M. Du PlessisAbstract:Case summary A 1-year-old, female spayed, domestic shorthair, indoor cat on the island of St Kitts was diagnosed with platynosomiasis, infection with a feline-specific liver fluke, and treated with praziquantel at the marketed dose for tapeworms (5 mg/kg; actual calculated dose 5.75 mg/kg). Serial fecal analyses showed that egg counts decreased to zero within 10 days of treatment but re-emerged at day 17 and persisted at low levels until a second treatment was administered on day 78. After the second treatment, all fecal samples (n = 15) from day 85 to day 350 post-initial treatment were negative for Platynosomum ova. Relevance and novel information Treatment of platynosomiasis is poorly documented; no drugs are labeled for use against Platynosomum and the efficacy of suggested treatments is unknown. Using 5.75 mg/kg once, a dose that is significantly lower than published recommended doses for platynosomiasis, egg counts initially disappeared but re-emerged and persisted at low levels until a second treatment was administered. We hypothesize that immature forms may not have been killed and subsequently matured to produce eggs, or that the one-time dose may not have been completely effective at eliminating all adult flukes. However, administering praziquantel at 5.75 mg/kg twice, several weeks apart, appeared to be effective in treating this cat with platynosomiasis, as evidenced by monitoring of fecal egg counts over the course of 350 days.
Liza S Köster - One of the best experts on this subject based on the ideXlab platform.
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percutaneous ultrasound guided cholecystocentesis and bile analysis for the detection of Platynosomum spp induced cholangitis in cats
Journal of Veterinary Internal Medicine, 2016Co-Authors: Liza S Köster, Oscar Illanes, C. Lathroum, K. Neuville, Jennifer K. KetzisAbstract:Background Examination of bile could be useful to diagnose Platynosomum spp.-induced cholangitis in cats. Obtaining bile via percutaneous ultrasound-guided cholecystocentesis (PUC) is possible but raises safety concerns in cats with severe cholecystitis. Objectives The objectives of this study were to investigate the use of PUC to collect bile samples from cats with known platynosomosis and to determine if bile analysis could be a diagnostic test. Animals Twenty-seven free-roaming cats positive for Platynosomum spp. eggs via fecal examination. Methods In this prospective study, fecal egg counts were performed by double centrifugation with Sheather's solution. Bile was collected using PUC from anesthetized cats. Egg counts in bile were performed with a stereoscope. Euthanasia and postmortem examination were performed immediately after PUC. Results All cats had ultrasound (US) evidence of cholangitis or cholecystitis. Thirty-nine PUCs were performed with 14 cats having 2 PUCs 12 or 24 days apart. Postmortem examinations showed no overt gallbladder damage or leakage but fresh blood was noted in the gallbladder lumen of 3 cats. Median Platynosomum spp. egg counts were higher in bile (1450 eggs/mL; IQR, 400; 5138 eggs/mL) as compared to feces (46 eggs/mL; IQR, 10; 107 eggs/mL) (P < .001). Conclusion and Clinical Importance Bile egg count analysis is an alternative method with higher egg counts as compared to fecal egg count analysis for the diagnosis of platynosomosis. Obtaining bile via US guidance is technically feasible and safe in cats with cholangitis/cholecystitis. Cholecystocentesis and bile analysis are especially relevant for those cats with chronic cholangitis/cholecystitis and negative fecal egg counts for Platynosomum.
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Percutaneous Ultrasound‐guided Cholecystocentesis and Bile Analysis for the Detection of Platynosomum spp.‐Induced Cholangitis in Cats
Journal of veterinary internal medicine, 2016Co-Authors: Liza S Köster, Oscar Illanes, C. Lathroum, K. Neuville, Jennifer K. KetzisAbstract:Background Examination of bile could be useful to diagnose Platynosomum spp.-induced cholangitis in cats. Obtaining bile via percutaneous ultrasound-guided cholecystocentesis (PUC) is possible but raises safety concerns in cats with severe cholecystitis. Objectives The objectives of this study were to investigate the use of PUC to collect bile samples from cats with known platynosomosis and to determine if bile analysis could be a diagnostic test. Animals Twenty-seven free-roaming cats positive for Platynosomum spp. eggs via fecal examination. Methods In this prospective study, fecal egg counts were performed by double centrifugation with Sheather's solution. Bile was collected using PUC from anesthetized cats. Egg counts in bile were performed with a stereoscope. Euthanasia and postmortem examination were performed immediately after PUC. Results All cats had ultrasound (US) evidence of cholangitis or cholecystitis. Thirty-nine PUCs were performed with 14 cats having 2 PUCs 12 or 24 days apart. Postmortem examinations showed no overt gallbladder damage or leakage but fresh blood was noted in the gallbladder lumen of 3 cats. Median Platynosomum spp. egg counts were higher in bile (1450 eggs/mL; IQR, 400; 5138 eggs/mL) as compared to feces (46 eggs/mL; IQR, 10; 107 eggs/mL) (P < .001). Conclusion and Clinical Importance Bile egg count analysis is an alternative method with higher egg counts as compared to fecal egg count analysis for the diagnosis of platynosomosis. Obtaining bile via US guidance is technically feasible and safe in cats with cholangitis/cholecystitis. Cholecystocentesis and bile analysis are especially relevant for those cats with chronic cholangitis/cholecystitis and negative fecal egg counts for Platynosomum.
C. Lathroum - One of the best experts on this subject based on the ideXlab platform.
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percutaneous ultrasound guided cholecystocentesis and bile analysis for the detection of Platynosomum spp induced cholangitis in cats
Journal of Veterinary Internal Medicine, 2016Co-Authors: Liza S Köster, Oscar Illanes, C. Lathroum, K. Neuville, Jennifer K. KetzisAbstract:Background Examination of bile could be useful to diagnose Platynosomum spp.-induced cholangitis in cats. Obtaining bile via percutaneous ultrasound-guided cholecystocentesis (PUC) is possible but raises safety concerns in cats with severe cholecystitis. Objectives The objectives of this study were to investigate the use of PUC to collect bile samples from cats with known platynosomosis and to determine if bile analysis could be a diagnostic test. Animals Twenty-seven free-roaming cats positive for Platynosomum spp. eggs via fecal examination. Methods In this prospective study, fecal egg counts were performed by double centrifugation with Sheather's solution. Bile was collected using PUC from anesthetized cats. Egg counts in bile were performed with a stereoscope. Euthanasia and postmortem examination were performed immediately after PUC. Results All cats had ultrasound (US) evidence of cholangitis or cholecystitis. Thirty-nine PUCs were performed with 14 cats having 2 PUCs 12 or 24 days apart. Postmortem examinations showed no overt gallbladder damage or leakage but fresh blood was noted in the gallbladder lumen of 3 cats. Median Platynosomum spp. egg counts were higher in bile (1450 eggs/mL; IQR, 400; 5138 eggs/mL) as compared to feces (46 eggs/mL; IQR, 10; 107 eggs/mL) (P < .001). Conclusion and Clinical Importance Bile egg count analysis is an alternative method with higher egg counts as compared to fecal egg count analysis for the diagnosis of platynosomosis. Obtaining bile via US guidance is technically feasible and safe in cats with cholangitis/cholecystitis. Cholecystocentesis and bile analysis are especially relevant for those cats with chronic cholangitis/cholecystitis and negative fecal egg counts for Platynosomum.
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Percutaneous Ultrasound‐guided Cholecystocentesis and Bile Analysis for the Detection of Platynosomum spp.‐Induced Cholangitis in Cats
Journal of veterinary internal medicine, 2016Co-Authors: Liza S Köster, Oscar Illanes, C. Lathroum, K. Neuville, Jennifer K. KetzisAbstract:Background Examination of bile could be useful to diagnose Platynosomum spp.-induced cholangitis in cats. Obtaining bile via percutaneous ultrasound-guided cholecystocentesis (PUC) is possible but raises safety concerns in cats with severe cholecystitis. Objectives The objectives of this study were to investigate the use of PUC to collect bile samples from cats with known platynosomosis and to determine if bile analysis could be a diagnostic test. Animals Twenty-seven free-roaming cats positive for Platynosomum spp. eggs via fecal examination. Methods In this prospective study, fecal egg counts were performed by double centrifugation with Sheather's solution. Bile was collected using PUC from anesthetized cats. Egg counts in bile were performed with a stereoscope. Euthanasia and postmortem examination were performed immediately after PUC. Results All cats had ultrasound (US) evidence of cholangitis or cholecystitis. Thirty-nine PUCs were performed with 14 cats having 2 PUCs 12 or 24 days apart. Postmortem examinations showed no overt gallbladder damage or leakage but fresh blood was noted in the gallbladder lumen of 3 cats. Median Platynosomum spp. egg counts were higher in bile (1450 eggs/mL; IQR, 400; 5138 eggs/mL) as compared to feces (46 eggs/mL; IQR, 10; 107 eggs/mL) (P < .001). Conclusion and Clinical Importance Bile egg count analysis is an alternative method with higher egg counts as compared to fecal egg count analysis for the diagnosis of platynosomosis. Obtaining bile via US guidance is technically feasible and safe in cats with cholangitis/cholecystitis. Cholecystocentesis and bile analysis are especially relevant for those cats with chronic cholangitis/cholecystitis and negative fecal egg counts for Platynosomum.
Oscar Illanes - One of the best experts on this subject based on the ideXlab platform.
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percutaneous ultrasound guided cholecystocentesis and bile analysis for the detection of Platynosomum spp induced cholangitis in cats
Journal of Veterinary Internal Medicine, 2016Co-Authors: Liza S Köster, Oscar Illanes, C. Lathroum, K. Neuville, Jennifer K. KetzisAbstract:Background Examination of bile could be useful to diagnose Platynosomum spp.-induced cholangitis in cats. Obtaining bile via percutaneous ultrasound-guided cholecystocentesis (PUC) is possible but raises safety concerns in cats with severe cholecystitis. Objectives The objectives of this study were to investigate the use of PUC to collect bile samples from cats with known platynosomosis and to determine if bile analysis could be a diagnostic test. Animals Twenty-seven free-roaming cats positive for Platynosomum spp. eggs via fecal examination. Methods In this prospective study, fecal egg counts were performed by double centrifugation with Sheather's solution. Bile was collected using PUC from anesthetized cats. Egg counts in bile were performed with a stereoscope. Euthanasia and postmortem examination were performed immediately after PUC. Results All cats had ultrasound (US) evidence of cholangitis or cholecystitis. Thirty-nine PUCs were performed with 14 cats having 2 PUCs 12 or 24 days apart. Postmortem examinations showed no overt gallbladder damage or leakage but fresh blood was noted in the gallbladder lumen of 3 cats. Median Platynosomum spp. egg counts were higher in bile (1450 eggs/mL; IQR, 400; 5138 eggs/mL) as compared to feces (46 eggs/mL; IQR, 10; 107 eggs/mL) (P < .001). Conclusion and Clinical Importance Bile egg count analysis is an alternative method with higher egg counts as compared to fecal egg count analysis for the diagnosis of platynosomosis. Obtaining bile via US guidance is technically feasible and safe in cats with cholangitis/cholecystitis. Cholecystocentesis and bile analysis are especially relevant for those cats with chronic cholangitis/cholecystitis and negative fecal egg counts for Platynosomum.
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Percutaneous Ultrasound‐guided Cholecystocentesis and Bile Analysis for the Detection of Platynosomum spp.‐Induced Cholangitis in Cats
Journal of veterinary internal medicine, 2016Co-Authors: Liza S Köster, Oscar Illanes, C. Lathroum, K. Neuville, Jennifer K. KetzisAbstract:Background Examination of bile could be useful to diagnose Platynosomum spp.-induced cholangitis in cats. Obtaining bile via percutaneous ultrasound-guided cholecystocentesis (PUC) is possible but raises safety concerns in cats with severe cholecystitis. Objectives The objectives of this study were to investigate the use of PUC to collect bile samples from cats with known platynosomosis and to determine if bile analysis could be a diagnostic test. Animals Twenty-seven free-roaming cats positive for Platynosomum spp. eggs via fecal examination. Methods In this prospective study, fecal egg counts were performed by double centrifugation with Sheather's solution. Bile was collected using PUC from anesthetized cats. Egg counts in bile were performed with a stereoscope. Euthanasia and postmortem examination were performed immediately after PUC. Results All cats had ultrasound (US) evidence of cholangitis or cholecystitis. Thirty-nine PUCs were performed with 14 cats having 2 PUCs 12 or 24 days apart. Postmortem examinations showed no overt gallbladder damage or leakage but fresh blood was noted in the gallbladder lumen of 3 cats. Median Platynosomum spp. egg counts were higher in bile (1450 eggs/mL; IQR, 400; 5138 eggs/mL) as compared to feces (46 eggs/mL; IQR, 10; 107 eggs/mL) (P < .001). Conclusion and Clinical Importance Bile egg count analysis is an alternative method with higher egg counts as compared to fecal egg count analysis for the diagnosis of platynosomosis. Obtaining bile via US guidance is technically feasible and safe in cats with cholangitis/cholecystitis. Cholecystocentesis and bile analysis are especially relevant for those cats with chronic cholangitis/cholecystitis and negative fecal egg counts for Platynosomum.
K. Neuville - One of the best experts on this subject based on the ideXlab platform.
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percutaneous ultrasound guided cholecystocentesis and bile analysis for the detection of Platynosomum spp induced cholangitis in cats
Journal of Veterinary Internal Medicine, 2016Co-Authors: Liza S Köster, Oscar Illanes, C. Lathroum, K. Neuville, Jennifer K. KetzisAbstract:Background Examination of bile could be useful to diagnose Platynosomum spp.-induced cholangitis in cats. Obtaining bile via percutaneous ultrasound-guided cholecystocentesis (PUC) is possible but raises safety concerns in cats with severe cholecystitis. Objectives The objectives of this study were to investigate the use of PUC to collect bile samples from cats with known platynosomosis and to determine if bile analysis could be a diagnostic test. Animals Twenty-seven free-roaming cats positive for Platynosomum spp. eggs via fecal examination. Methods In this prospective study, fecal egg counts were performed by double centrifugation with Sheather's solution. Bile was collected using PUC from anesthetized cats. Egg counts in bile were performed with a stereoscope. Euthanasia and postmortem examination were performed immediately after PUC. Results All cats had ultrasound (US) evidence of cholangitis or cholecystitis. Thirty-nine PUCs were performed with 14 cats having 2 PUCs 12 or 24 days apart. Postmortem examinations showed no overt gallbladder damage or leakage but fresh blood was noted in the gallbladder lumen of 3 cats. Median Platynosomum spp. egg counts were higher in bile (1450 eggs/mL; IQR, 400; 5138 eggs/mL) as compared to feces (46 eggs/mL; IQR, 10; 107 eggs/mL) (P < .001). Conclusion and Clinical Importance Bile egg count analysis is an alternative method with higher egg counts as compared to fecal egg count analysis for the diagnosis of platynosomosis. Obtaining bile via US guidance is technically feasible and safe in cats with cholangitis/cholecystitis. Cholecystocentesis and bile analysis are especially relevant for those cats with chronic cholangitis/cholecystitis and negative fecal egg counts for Platynosomum.
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Percutaneous Ultrasound‐guided Cholecystocentesis and Bile Analysis for the Detection of Platynosomum spp.‐Induced Cholangitis in Cats
Journal of veterinary internal medicine, 2016Co-Authors: Liza S Köster, Oscar Illanes, C. Lathroum, K. Neuville, Jennifer K. KetzisAbstract:Background Examination of bile could be useful to diagnose Platynosomum spp.-induced cholangitis in cats. Obtaining bile via percutaneous ultrasound-guided cholecystocentesis (PUC) is possible but raises safety concerns in cats with severe cholecystitis. Objectives The objectives of this study were to investigate the use of PUC to collect bile samples from cats with known platynosomosis and to determine if bile analysis could be a diagnostic test. Animals Twenty-seven free-roaming cats positive for Platynosomum spp. eggs via fecal examination. Methods In this prospective study, fecal egg counts were performed by double centrifugation with Sheather's solution. Bile was collected using PUC from anesthetized cats. Egg counts in bile were performed with a stereoscope. Euthanasia and postmortem examination were performed immediately after PUC. Results All cats had ultrasound (US) evidence of cholangitis or cholecystitis. Thirty-nine PUCs were performed with 14 cats having 2 PUCs 12 or 24 days apart. Postmortem examinations showed no overt gallbladder damage or leakage but fresh blood was noted in the gallbladder lumen of 3 cats. Median Platynosomum spp. egg counts were higher in bile (1450 eggs/mL; IQR, 400; 5138 eggs/mL) as compared to feces (46 eggs/mL; IQR, 10; 107 eggs/mL) (P < .001). Conclusion and Clinical Importance Bile egg count analysis is an alternative method with higher egg counts as compared to fecal egg count analysis for the diagnosis of platynosomosis. Obtaining bile via US guidance is technically feasible and safe in cats with cholangitis/cholecystitis. Cholecystocentesis and bile analysis are especially relevant for those cats with chronic cholangitis/cholecystitis and negative fecal egg counts for Platynosomum.