The Experts below are selected from a list of 360 Experts worldwide ranked by ideXlab platform

Karl R Koehler - One of the best experts on this subject based on the ideXlab platform.

  • hair follicle development in mouse Pluripotent Stem Cell derived skin organoids
    Cell Reports, 2018
    Co-Authors: Jiyoon Lee, Robert Bӧscke, Peiciao Tang, Byron H Hartman, Stefan Heller, Karl R Koehler
    Abstract:

    The mammalian hair follicle arises during embryonic development from coordinated interactions between the epidermis and dermis. It is currently unclear how to recapitulate hair follicle induction in Pluripotent Stem Cell cultures for use in basic research studies or in vitro drug testing. To date, generation of hair follicles in vitro has only been possible using primary Cells isolated from embryonic skin, cultured alone or in a co-culture with Stem Cell-derived Cells, combined with in vivo transplantation. Here, we describe the derivation of skin organoids, constituting epidermal and dermal layers, from a homogeneous population of mouse Pluripotent Stem Cells in a 3D culture. We show that skin organoids spontaneously produce de novo hair follicles in a process that mimics normal embryonic hair folliculogenesis. This in vitro model of skin development will be useful for studying mechanisms of hair follicle induction, evaluating hair growth or inhibitory drugs, and modeling skin diseases.

  • Hair Follicle Development in Mouse Pluripotent Stem Cell-Derived Skin Organoids
    Elsevier, 2018
    Co-Authors: Jiyoon Lee, Robert Bӧscke, Peiciao Tang, Byron H Hartman, Stefan Heller, Karl R Koehler
    Abstract:

    Summary: The mammalian hair follicle arises during embryonic development from coordinated interactions between the epidermis and dermis. It is currently unclear how to recapitulate hair follicle induction in Pluripotent Stem Cell cultures for use in basic research studies or in vitro drug testing. To date, generation of hair follicles in vitro has only been possible using primary Cells isolated from embryonic skin, cultured alone or in a co-culture with Stem Cell-derived Cells, combined with in vivo transplantation. Here, we describe the derivation of skin organoids, constituting epidermal and dermal layers, from a homogeneous population of mouse Pluripotent Stem Cells in a 3D culture. We show that skin organoids spontaneously produce de novo hair follicles in a process that mimics normal embryonic hair folliculogenesis. This in vitro model of skin development will be useful for studying mechanisms of hair follicle induction, evaluating hair growth or inhibitory drugs, and modeling skin diseases. : Lee at el. describe a defined in vitro 3D culture syStem that generates skin organoids from mouse Pluripotent Stem Cells. The skin organoids contain self-organized skin layers and skin appendages, including hair follicles, sebaceous glands, and adipocytes. Keywords: Pluripotent Stem Cells, organoids, skin, epidermis, dermis, hair follicle, skin appendages, 3D cultur

Masumi Hirabayashi - One of the best experts on this subject based on the ideXlab platform.

  • Generation of Pluripotent Stem Cell-derived mouse kidneys in Sall1-targeted anephric rats
    Nature communications, 2019
    Co-Authors: Teppei Goto, Hiromasa Hara, Makoto Sanbo, Hideki Masaki, Hideyuki Sato, Tomoyuki Yamaguchi, Shinichi Hochi, Toshihiro Kobayashi, Hiromitsu Nakauchi, Masumi Hirabayashi
    Abstract:

    Regeneration of human kidneys in animal models would help combat the severe shortage of donors in transplantation therapy. Previously, we demonstrated by interspecific blastocyst complementation between mouse and rats, generation of Pluripotent Stem Cell (PSC)-derived functional pancreas, in apancreatic Pdx1 mutant mice. We, however, were unable to obtain rat PSC-derived kidneys in anephric Sall1 mutant mice, likely due to the poor contribution of rat PSCs to the mouse metanephric mesenchyme, a nephron progenitor. Here, conversely, we show that mouse PSCs can efficiently differentiate into the metanephric mesenchyme in rat, allowing the generation of mouse PSC-derived kidney in anephric Sall1 mutant rat. Glomerular epithelium and renal tubules in the kidneys are entirely composed of mouse PSC-derived Cells expressing key functional markers. Importantly, the ureter-bladder junction is normally formed. These data provide proof-of-principle for interspecific blastocyst complementation as a viable approach for kidney generation.

  • Generation of Pluripotent Stem Cell-derived mouse kidneys in Sall1-targeted anephric rats
    Nature Publishing Group, 2019
    Co-Authors: Teppei Goto, Hiromasa Hara, Makoto Sanbo, Hideki Masaki, Hideyuki Sato, Tomoyuki Yamaguchi, Shinichi Hochi, Toshihiro Kobayashi, Hiromitsu Nakauchi, Masumi Hirabayashi
    Abstract:

    The use of Pluripotent-Stem Cell derived organs for transplantation would be promising, if organs can be grown in a suitable host. Here, the authors use interspecific blastocyst complementation to generate a mouse Pluripotent Stem Cell-derived kidney in anephric Sall1 mutant rats

Dhruv Sareen - One of the best experts on this subject based on the ideXlab platform.

  • endocrine disruptors induce perturbations in endoplasmic reticulum and mitochondria of human Pluripotent Stem Cell derivatives
    Nature Communications, 2017
    Co-Authors: Uthra Rajamani, Andrew R Gross, Camille Ocampo, Allen M Andres, Roberta A Gottlieb, Dhruv Sareen
    Abstract:

    Persistent exposure to man-made endocrine disrupting chemicals during fetal endocrine development may lead to disruption of metabolic homeostasis contributing to childhood obesity. Limited Cellular platforms exist to test endocrine disrupting chemical-induced developmental abnormalities in human endocrine tissues. Here we use an human-induced Pluripotent Stem Cell-based platform to demonstrate adverse impacts of obesogenic endocrine disrupting chemicals in the developing endocrine syStem. We delineate the effects upon physiological low-dose exposure to ubiquitous endocrine disrupting chemicals including, perfluoro-octanoic acid, tributyltin, and butylhydroxytoluene, in endocrine-active human-induced Pluripotent Stem Cell-derived foregut epithelial Cells and hypothalamic neurons. Endocrine disrupting chemicals induce endoplasmic reticulum stress, perturb NF-κB, and p53 signaling, and diminish mitochondrial respiratory gene expression, spare respiratory capacity, and ATP levels. As a result, normal production and secretion of appetite control hormones, PYY, α-MSH, and CART, are hampered. Blocking NF-κB rescues endocrine disrupting chemical-induced aberrant mitochondrial phenotypes and endocrine dysregulation, but not ER-stress and p53-phosphorylation changes. Harmful chemicals that disrupt the endocrine syStem and hormone regulation have been associated with obesity. Here the authors apply a human Pluripotent Stem Cell-based platform to study the effects of such compounds on developing gut endocrine and neuroendocrine syStems.

  • retraction note endocrine disruptors induce perturbations in endoplasmic reticulum and mitochondria of human Pluripotent Stem Cell derivatives
    Nature Communications, 2017
    Co-Authors: Uthra Rajamani, Andrew R Gross, Camille Ocampo, Allen M Andres, Roberta A Gottlieb, Dhruv Sareen
    Abstract:

    Persistent exposure to man-made endocrine disrupting chemicals during fetal endocrine development may lead to disruption of metabolic homeostasis contributing to childhood obesity. Limited Cellular platforms exist to test endocrine disrupting chemical-induced developmental abnormalities in human endocrine tissues. Here we use an human-induced Pluripotent Stem Cell-based platform to demonstrate adverse impacts of obesogenic endocrine disrupting chemicals in the developing endocrine syStem. We delineate the effects upon physiological low-dose exposure to ubiquitous endocrine disrupting chemicals including, perfluoro-octanoic acid, tributyltin, and butylhydroxytoluene, in endocrine-active human-induced Pluripotent Stem Cell-derived foregut epithelial Cells and hypothalamic neurons. Endocrine disrupting chemicals induce endoplasmic reticulum stress, perturb NF-κB, and p53 signaling, and diminish mitochondrial respiratory gene expression, spare respiratory capacity, and ATP levels. As a result, normal production and secretion of appetite control hormones, PYY, α-MSH, and CART, are hampered. Blocking NF-κB rescues endocrine disrupting chemical-induced aberrant mitochondrial phenotypes and endocrine dysregulation, but not ER-stress and p53-phosphorylation changes. Harmful chemicals that disrupt the endocrine syStem and hormone regulation have been associated with obesity. Here the authors apply a human Pluripotent Stem Cell-based platform to study the effects of such compounds on developing gut endocrine and neuroendocrine syStems.

Camille Ocampo - One of the best experts on this subject based on the ideXlab platform.

  • endocrine disruptors induce perturbations in endoplasmic reticulum and mitochondria of human Pluripotent Stem Cell derivatives
    Nature Communications, 2017
    Co-Authors: Uthra Rajamani, Andrew R Gross, Camille Ocampo, Allen M Andres, Roberta A Gottlieb, Dhruv Sareen
    Abstract:

    Persistent exposure to man-made endocrine disrupting chemicals during fetal endocrine development may lead to disruption of metabolic homeostasis contributing to childhood obesity. Limited Cellular platforms exist to test endocrine disrupting chemical-induced developmental abnormalities in human endocrine tissues. Here we use an human-induced Pluripotent Stem Cell-based platform to demonstrate adverse impacts of obesogenic endocrine disrupting chemicals in the developing endocrine syStem. We delineate the effects upon physiological low-dose exposure to ubiquitous endocrine disrupting chemicals including, perfluoro-octanoic acid, tributyltin, and butylhydroxytoluene, in endocrine-active human-induced Pluripotent Stem Cell-derived foregut epithelial Cells and hypothalamic neurons. Endocrine disrupting chemicals induce endoplasmic reticulum stress, perturb NF-κB, and p53 signaling, and diminish mitochondrial respiratory gene expression, spare respiratory capacity, and ATP levels. As a result, normal production and secretion of appetite control hormones, PYY, α-MSH, and CART, are hampered. Blocking NF-κB rescues endocrine disrupting chemical-induced aberrant mitochondrial phenotypes and endocrine dysregulation, but not ER-stress and p53-phosphorylation changes. Harmful chemicals that disrupt the endocrine syStem and hormone regulation have been associated with obesity. Here the authors apply a human Pluripotent Stem Cell-based platform to study the effects of such compounds on developing gut endocrine and neuroendocrine syStems.

  • retraction note endocrine disruptors induce perturbations in endoplasmic reticulum and mitochondria of human Pluripotent Stem Cell derivatives
    Nature Communications, 2017
    Co-Authors: Uthra Rajamani, Andrew R Gross, Camille Ocampo, Allen M Andres, Roberta A Gottlieb, Dhruv Sareen
    Abstract:

    Persistent exposure to man-made endocrine disrupting chemicals during fetal endocrine development may lead to disruption of metabolic homeostasis contributing to childhood obesity. Limited Cellular platforms exist to test endocrine disrupting chemical-induced developmental abnormalities in human endocrine tissues. Here we use an human-induced Pluripotent Stem Cell-based platform to demonstrate adverse impacts of obesogenic endocrine disrupting chemicals in the developing endocrine syStem. We delineate the effects upon physiological low-dose exposure to ubiquitous endocrine disrupting chemicals including, perfluoro-octanoic acid, tributyltin, and butylhydroxytoluene, in endocrine-active human-induced Pluripotent Stem Cell-derived foregut epithelial Cells and hypothalamic neurons. Endocrine disrupting chemicals induce endoplasmic reticulum stress, perturb NF-κB, and p53 signaling, and diminish mitochondrial respiratory gene expression, spare respiratory capacity, and ATP levels. As a result, normal production and secretion of appetite control hormones, PYY, α-MSH, and CART, are hampered. Blocking NF-κB rescues endocrine disrupting chemical-induced aberrant mitochondrial phenotypes and endocrine dysregulation, but not ER-stress and p53-phosphorylation changes. Harmful chemicals that disrupt the endocrine syStem and hormone regulation have been associated with obesity. Here the authors apply a human Pluripotent Stem Cell-based platform to study the effects of such compounds on developing gut endocrine and neuroendocrine syStems.

Jiyoon Lee - One of the best experts on this subject based on the ideXlab platform.

  • hair follicle development in mouse Pluripotent Stem Cell derived skin organoids
    Cell Reports, 2018
    Co-Authors: Jiyoon Lee, Robert Bӧscke, Peiciao Tang, Byron H Hartman, Stefan Heller, Karl R Koehler
    Abstract:

    The mammalian hair follicle arises during embryonic development from coordinated interactions between the epidermis and dermis. It is currently unclear how to recapitulate hair follicle induction in Pluripotent Stem Cell cultures for use in basic research studies or in vitro drug testing. To date, generation of hair follicles in vitro has only been possible using primary Cells isolated from embryonic skin, cultured alone or in a co-culture with Stem Cell-derived Cells, combined with in vivo transplantation. Here, we describe the derivation of skin organoids, constituting epidermal and dermal layers, from a homogeneous population of mouse Pluripotent Stem Cells in a 3D culture. We show that skin organoids spontaneously produce de novo hair follicles in a process that mimics normal embryonic hair folliculogenesis. This in vitro model of skin development will be useful for studying mechanisms of hair follicle induction, evaluating hair growth or inhibitory drugs, and modeling skin diseases.

  • Hair Follicle Development in Mouse Pluripotent Stem Cell-Derived Skin Organoids
    Elsevier, 2018
    Co-Authors: Jiyoon Lee, Robert Bӧscke, Peiciao Tang, Byron H Hartman, Stefan Heller, Karl R Koehler
    Abstract:

    Summary: The mammalian hair follicle arises during embryonic development from coordinated interactions between the epidermis and dermis. It is currently unclear how to recapitulate hair follicle induction in Pluripotent Stem Cell cultures for use in basic research studies or in vitro drug testing. To date, generation of hair follicles in vitro has only been possible using primary Cells isolated from embryonic skin, cultured alone or in a co-culture with Stem Cell-derived Cells, combined with in vivo transplantation. Here, we describe the derivation of skin organoids, constituting epidermal and dermal layers, from a homogeneous population of mouse Pluripotent Stem Cells in a 3D culture. We show that skin organoids spontaneously produce de novo hair follicles in a process that mimics normal embryonic hair folliculogenesis. This in vitro model of skin development will be useful for studying mechanisms of hair follicle induction, evaluating hair growth or inhibitory drugs, and modeling skin diseases. : Lee at el. describe a defined in vitro 3D culture syStem that generates skin organoids from mouse Pluripotent Stem Cells. The skin organoids contain self-organized skin layers and skin appendages, including hair follicles, sebaceous glands, and adipocytes. Keywords: Pluripotent Stem Cells, organoids, skin, epidermis, dermis, hair follicle, skin appendages, 3D cultur