The Experts below are selected from a list of 30621 Experts worldwide ranked by ideXlab platform

Massart Sébastien - One of the best experts on this subject based on the ideXlab platform.

  • Identification, molecular and biological characterization of two novel secovirids in wild grass species in Belgium
    2021
    Co-Authors: Maclot François, Filloux Denis, Debue Virginie, Blouin Arnaud, Fontdevila Pareta Núria, Tamisier Lucie, Massart Sébastien
    Abstract:

    High throughput sequencing was performed on virion-associated nucleic acids (VANA) from a pool of fifty asymptomatic rough bluegrasses (Poa trivialis L.) collected in a Belgian grazed pasture. Bioinformatics analyses produced some contigs presenting similarities with secovirid genomes, in particular nepoviruses and waikaviruses. Three distinct positive-sense single-stranded RNAs including 5’ and 3’ UTR were reconstructed and they represented two novel viruses infecting rough bluegrass, for which the provisional names Poaceae Liege nepovirus A (PoLNVA, 7298 nt for RNA1 and 4263 nt for RNA2) and Poaceae Liege virus 1 (PoLV1, 11,623 nt) were proposed. Compared to other Secoviridae members, the highest amino acid identity reached 90.7 % and 66.7 % between PoLNVA and nepoviruses for the Pro-Pol and CP regions respectively, while PoLV1 presented the highest amino acid identity with waikaviruses but with lower identities, i.e. 41.2 % for Pro-Pol and 25.8 % for CP regions, far below the ICTV demarcation criteria for novel secovirid. Based on sequence identity and phylogenetic analyses, PoLNVA was proposed to belong to the genus Nepovirus and PoLV1 as an unclassified secovirids. Detection of the two novel viruses was confirmed in high prevalence in rough bluegrass and ten other wild Poaceae species (Agropyron repens, Agrostis capillaris, Apera spica-venti, Anthoxanthum odoratum, Cynosorus cristatus, Festuca rubra, Holcus lanatus, Lolium perenne, Phleum bertolini and Phleum pratense) by RT-PCR and Sanger sequencing, revealing a diverse host range within Poaceae for these novel secovirids. Seed transmission was evaluated and confirmed for PoLNVA.Peer reviewe

  • Identification, molecular and biological characterization of two novel secovirids in wild grass species in Belgium
    'Elsevier BV', 2021
    Co-Authors: Maclot François, Filloux Denis, Debue Virginie, Blouin Arnaud, Fontdevila Pareta Núria, Tamisier Lucie, Massart Sébastien
    Abstract:

    International audienceHigh throughput sequencing was performed on virion-associated nucleic acids (VANA) from a pool of fifty asymptomatic rough bluegrasses (Poa trivialis L.) collected in a Belgian grazed pasture. Bioinformatics analyses produced some contigs presenting similarities with secovirid genomes, in particular nepoviruses and waikaviruses. Three distinct positive-sense single-stranded RNAs including 5? and 3? UTR were reconstructed and they represented two novel viruses infecting rough bluegrass, for which the provisional names Poaceae Liege nepovirus A (PoLNVA, 7298 nt for RNA1 and 4263 nt for RNA2) and Poaceae Liege virus 1 (PoLV1, 11,623 nt) were proposed. Compared to other Secoviridae members, the highest amino acid identity reached 90.7 % and 66.7 % between PoLNVA and nepoviruses for the Pro-Pol and CP regions respectively, while PoLV1 presented the highest amino acid identity with waikaviruses but with lower identities, i.e. 41.2 % for Pro-Pol and 25.8 % for CP regions, far below the ICTV demarcation criteria for novel secovirid. Based on sequence identity and phylogenetic analyses, PoLNVA was proposed to belong to the genus Nepovirus and PoLV1 as an unclassified secovirids. Detection of the two novel viruses was confirmed in high prevalence in rough bluegrass and ten other wild Poaceae species (Agropyron repens, Agrostis capillaris, Apera spica-venti, Anthoxanthum odoratum, Cynosorus cristatus, Festuca rubra, Holcus lanatus, Lolium perenne, Phleum bertolini and Phleum pratense) by RT-PCR and Sanger sequencing, revealing a diverse host range within Poaceae for these novel secovirids. Seed transmission was evaluated and confirmed for PoLNVA

  • Exploration de la diversité et richesse virale dans des communautés végétales de Poacées contrastées
    2020
    Co-Authors: Maclot François, Filloux Denis, Candresse Thierry, Malmstrom Carolyn, Massart Sébastien
    Abstract:

    audience: researcherPrior to plant domestication, it is hypothesized that plant viruses were co-evolving with uncultivated plants growing in mixed species communities, thereby resulting in complex interactions (antagonism, commensalism, mutualism). Development of agriculture further deeply modified natural ecosystems, land use and dynamics of virus-plant interactions, which has fostered occurrence of virus disease emergence events. In this context, we are conducting a study in the Natural Park “Burdinale-Mehaigne” (Belgium) using high throughput sequencing technologies in order to examine the impact of plant species diversity on the virome (e.g. the genomes of viral community) of Poaceae in contrasted plant communities (cereal monocultures, grazed pastures and natural grasslands). We adapted a virion-associated nucleic acids (VANA) metagenomics protocol to sequence at high throughput pools of 50 plant samples per plant community (50 samples reflecting plant species composition) and per plant species. Over two years, about 4,300 Poaceae plants (corresponding to 24 species) were sampled and bioinformatic analyses revealed presence of diverse viral communities in wild and cultivated Poaceae, even though they did not present any symptoms. These viruses belong to diverse families (e.g. Alphaflexiviridae, Luteoviridae, Partitiviridae, Totiviridae), infecting a large range of hosts within Poaceae and transmitted by different vectors (insects, mites, nematods) or seed-borne. More than 30 virus species were detected in the different plots and at least half of them are candidates for new virus species, revealing that virome in Poaceae-based agroecosystems and its impact on the dynamic of plant communities remain largely unexplored.Impact of ecosystem diversity on Poaceae virom

  • Exploration de la diversité et richesse virale dans des communautés végétales de Poacées contrastées
    2020
    Co-Authors: Maclot François, Filloux Denis, Candresse Thierry, Malmstrom Carolyn, Massart Sébastien
    Abstract:

    Prior to plant domestication, it is hypothesized that plant viruses were co-evolving with uncultivated plants growing in mixed species communities, thereby resulting in complex interactions (antagonism, commensalism, mutualism). Development of agriculture further deeply modified natural ecosystems, land use and dynamics of virus-plant interactions, which has fostered occurrence of virus disease emergence events. In this context, we are conducting a study in the Natural Park “Burdinale-Mehaigne” (Belgium) using high throughput sequencing technologies in order to examine the impact of plant species diversity on the virome (e.g. the genomes of viral community) of Poaceae in contrasted plant communities (cereal monocultures, grazed pastures and natural grasslands). We adapted a virion-associated nucleic acids (VANA) metagenomics protocol to sequence at high throughput pools of 50 plant samples per plant community (50 samples reflecting plant species composition) and per plant species. Over two years, about 4,300 Poaceae plants (corresponding to 24 species) were sampled and bioinformatic analyses revealed presence of diverse viral communities in wild and cultivated Poaceae, even though they did not present any symptoms. These viruses belong to diverse families (e.g. Alphaflexiviridae, Luteoviridae, Partitiviridae, Totiviridae), infecting a large range of hosts within Poaceae and transmitted by different vectors (insects, mites, nematods) or seed-borne. More than 30 virus species were detected in the different plots and at least half of them are candidates for new virus species, revealing that virome in Poaceae-based agroecosystems and its impact on the dynamic of plant communities remain largely unexplored

  • Les espèces végétales minoritaires peuvent jouer un rôle clé dans le virome des Poaceae à l'échelle des agroécosystèmes
    2019
    Co-Authors: Maclot François, Filloux Denis, Roumagnac Philippe, Candresse Thierry, Massart Sébastien
    Abstract:

    Prior to the domestication of plants, it is hypothesized that plant viruses were co-evolving with uncultivated plants growing in mixed species communities, which probably resulted in complex interactions (antagonism, commensalism, mutualism). The development of agriculture further deeply modified natural ecosystems and land use, creating agroecosystems composed by both cultivated and uncultivated areas. It is postulated that the advent of agriculture has modified the dynamics of virus-plant interactions, which has fostered the occurrence of virus disease emergence events. At the level of natural ecosystems, the relationships between plant communities’ diversity and plant virus diversity, the distribution of plant viruses and the interactions between viruses and their uncultivated hosts, have just started to be explored. In this context, we are conducting a study in the Natural Park “Burdinale-Mehaigne” (Belgium) using high throughput sequencing technologies in order to characterize the virome of Poaceae communities, including minor species, in contrasted agricultural ecosystems (cereal monocultures, grazed pastures and natural grasslands). We adapted a virion-associated nucleic acids (VANA) metagenomics protocol to sequence at high throughput pools of 50 plant samples per ecosystem (50 samples reflecting plant species composition) and per plant species. Over two years, about 4,300 Poaceae plants (corresponding to 24 species) were sampled and processed using the VANA metagenomics approach. The bioinformatic analyses revealed the presence of diverse viral communities in wild and cultivated Poaceae, even though they did not present any symptoms. These viruses belong to diverse families (e.g. Endornaviridae, Luteoviridae, Partitiviridae, Potyviridae, Reoviridae, Secoviridae), infecting a large range of hosts within the Poaceae and transmitted by different vectors (aphids, planthoppers, mites, nematods) or seed-borne. Interestingly, minor Poaceae species studied in grasslands contained a wide virus richness, sometimes greater than ecosystemic pools containing 8-11 major plant species. Moreover, some virus genera (e.g. Amalgavirus, Alphaendornavirus, Potyvirus, Sobemovirus) were found only in minor species, underlining the importance of minor plant species as virus reservoirs in wild ecosystems such as grasslands

Maclot François - One of the best experts on this subject based on the ideXlab platform.

  • Identification, molecular and biological characterization of two novel secovirids in wild grass species in Belgium
    2021
    Co-Authors: Maclot François, Filloux Denis, Debue Virginie, Blouin Arnaud, Fontdevila Pareta Núria, Tamisier Lucie, Massart Sébastien
    Abstract:

    High throughput sequencing was performed on virion-associated nucleic acids (VANA) from a pool of fifty asymptomatic rough bluegrasses (Poa trivialis L.) collected in a Belgian grazed pasture. Bioinformatics analyses produced some contigs presenting similarities with secovirid genomes, in particular nepoviruses and waikaviruses. Three distinct positive-sense single-stranded RNAs including 5’ and 3’ UTR were reconstructed and they represented two novel viruses infecting rough bluegrass, for which the provisional names Poaceae Liege nepovirus A (PoLNVA, 7298 nt for RNA1 and 4263 nt for RNA2) and Poaceae Liege virus 1 (PoLV1, 11,623 nt) were proposed. Compared to other Secoviridae members, the highest amino acid identity reached 90.7 % and 66.7 % between PoLNVA and nepoviruses for the Pro-Pol and CP regions respectively, while PoLV1 presented the highest amino acid identity with waikaviruses but with lower identities, i.e. 41.2 % for Pro-Pol and 25.8 % for CP regions, far below the ICTV demarcation criteria for novel secovirid. Based on sequence identity and phylogenetic analyses, PoLNVA was proposed to belong to the genus Nepovirus and PoLV1 as an unclassified secovirids. Detection of the two novel viruses was confirmed in high prevalence in rough bluegrass and ten other wild Poaceae species (Agropyron repens, Agrostis capillaris, Apera spica-venti, Anthoxanthum odoratum, Cynosorus cristatus, Festuca rubra, Holcus lanatus, Lolium perenne, Phleum bertolini and Phleum pratense) by RT-PCR and Sanger sequencing, revealing a diverse host range within Poaceae for these novel secovirids. Seed transmission was evaluated and confirmed for PoLNVA.Peer reviewe

  • Identification, molecular and biological characterization of two novel secovirids in wild grass species in Belgium
    'Elsevier BV', 2021
    Co-Authors: Maclot François, Filloux Denis, Debue Virginie, Blouin Arnaud, Fontdevila Pareta Núria, Tamisier Lucie, Massart Sébastien
    Abstract:

    International audienceHigh throughput sequencing was performed on virion-associated nucleic acids (VANA) from a pool of fifty asymptomatic rough bluegrasses (Poa trivialis L.) collected in a Belgian grazed pasture. Bioinformatics analyses produced some contigs presenting similarities with secovirid genomes, in particular nepoviruses and waikaviruses. Three distinct positive-sense single-stranded RNAs including 5? and 3? UTR were reconstructed and they represented two novel viruses infecting rough bluegrass, for which the provisional names Poaceae Liege nepovirus A (PoLNVA, 7298 nt for RNA1 and 4263 nt for RNA2) and Poaceae Liege virus 1 (PoLV1, 11,623 nt) were proposed. Compared to other Secoviridae members, the highest amino acid identity reached 90.7 % and 66.7 % between PoLNVA and nepoviruses for the Pro-Pol and CP regions respectively, while PoLV1 presented the highest amino acid identity with waikaviruses but with lower identities, i.e. 41.2 % for Pro-Pol and 25.8 % for CP regions, far below the ICTV demarcation criteria for novel secovirid. Based on sequence identity and phylogenetic analyses, PoLNVA was proposed to belong to the genus Nepovirus and PoLV1 as an unclassified secovirids. Detection of the two novel viruses was confirmed in high prevalence in rough bluegrass and ten other wild Poaceae species (Agropyron repens, Agrostis capillaris, Apera spica-venti, Anthoxanthum odoratum, Cynosorus cristatus, Festuca rubra, Holcus lanatus, Lolium perenne, Phleum bertolini and Phleum pratense) by RT-PCR and Sanger sequencing, revealing a diverse host range within Poaceae for these novel secovirids. Seed transmission was evaluated and confirmed for PoLNVA

  • Exploration de la diversité et richesse virale dans des communautés végétales de Poacées contrastées
    2020
    Co-Authors: Maclot François, Filloux Denis, Candresse Thierry, Malmstrom Carolyn, Massart Sébastien
    Abstract:

    audience: researcherPrior to plant domestication, it is hypothesized that plant viruses were co-evolving with uncultivated plants growing in mixed species communities, thereby resulting in complex interactions (antagonism, commensalism, mutualism). Development of agriculture further deeply modified natural ecosystems, land use and dynamics of virus-plant interactions, which has fostered occurrence of virus disease emergence events. In this context, we are conducting a study in the Natural Park “Burdinale-Mehaigne” (Belgium) using high throughput sequencing technologies in order to examine the impact of plant species diversity on the virome (e.g. the genomes of viral community) of Poaceae in contrasted plant communities (cereal monocultures, grazed pastures and natural grasslands). We adapted a virion-associated nucleic acids (VANA) metagenomics protocol to sequence at high throughput pools of 50 plant samples per plant community (50 samples reflecting plant species composition) and per plant species. Over two years, about 4,300 Poaceae plants (corresponding to 24 species) were sampled and bioinformatic analyses revealed presence of diverse viral communities in wild and cultivated Poaceae, even though they did not present any symptoms. These viruses belong to diverse families (e.g. Alphaflexiviridae, Luteoviridae, Partitiviridae, Totiviridae), infecting a large range of hosts within Poaceae and transmitted by different vectors (insects, mites, nematods) or seed-borne. More than 30 virus species were detected in the different plots and at least half of them are candidates for new virus species, revealing that virome in Poaceae-based agroecosystems and its impact on the dynamic of plant communities remain largely unexplored.Impact of ecosystem diversity on Poaceae virom

  • Exploration de la diversité et richesse virale dans des communautés végétales de Poacées contrastées
    2020
    Co-Authors: Maclot François, Filloux Denis, Candresse Thierry, Malmstrom Carolyn, Massart Sébastien
    Abstract:

    Prior to plant domestication, it is hypothesized that plant viruses were co-evolving with uncultivated plants growing in mixed species communities, thereby resulting in complex interactions (antagonism, commensalism, mutualism). Development of agriculture further deeply modified natural ecosystems, land use and dynamics of virus-plant interactions, which has fostered occurrence of virus disease emergence events. In this context, we are conducting a study in the Natural Park “Burdinale-Mehaigne” (Belgium) using high throughput sequencing technologies in order to examine the impact of plant species diversity on the virome (e.g. the genomes of viral community) of Poaceae in contrasted plant communities (cereal monocultures, grazed pastures and natural grasslands). We adapted a virion-associated nucleic acids (VANA) metagenomics protocol to sequence at high throughput pools of 50 plant samples per plant community (50 samples reflecting plant species composition) and per plant species. Over two years, about 4,300 Poaceae plants (corresponding to 24 species) were sampled and bioinformatic analyses revealed presence of diverse viral communities in wild and cultivated Poaceae, even though they did not present any symptoms. These viruses belong to diverse families (e.g. Alphaflexiviridae, Luteoviridae, Partitiviridae, Totiviridae), infecting a large range of hosts within Poaceae and transmitted by different vectors (insects, mites, nematods) or seed-borne. More than 30 virus species were detected in the different plots and at least half of them are candidates for new virus species, revealing that virome in Poaceae-based agroecosystems and its impact on the dynamic of plant communities remain largely unexplored

  • Les espèces végétales minoritaires peuvent jouer un rôle clé dans le virome des Poaceae à l'échelle des agroécosystèmes
    2019
    Co-Authors: Maclot François, Filloux Denis, Roumagnac Philippe, Candresse Thierry, Massart Sébastien
    Abstract:

    Prior to the domestication of plants, it is hypothesized that plant viruses were co-evolving with uncultivated plants growing in mixed species communities, which probably resulted in complex interactions (antagonism, commensalism, mutualism). The development of agriculture further deeply modified natural ecosystems and land use, creating agroecosystems composed by both cultivated and uncultivated areas. It is postulated that the advent of agriculture has modified the dynamics of virus-plant interactions, which has fostered the occurrence of virus disease emergence events. At the level of natural ecosystems, the relationships between plant communities’ diversity and plant virus diversity, the distribution of plant viruses and the interactions between viruses and their uncultivated hosts, have just started to be explored. In this context, we are conducting a study in the Natural Park “Burdinale-Mehaigne” (Belgium) using high throughput sequencing technologies in order to characterize the virome of Poaceae communities, including minor species, in contrasted agricultural ecosystems (cereal monocultures, grazed pastures and natural grasslands). We adapted a virion-associated nucleic acids (VANA) metagenomics protocol to sequence at high throughput pools of 50 plant samples per ecosystem (50 samples reflecting plant species composition) and per plant species. Over two years, about 4,300 Poaceae plants (corresponding to 24 species) were sampled and processed using the VANA metagenomics approach. The bioinformatic analyses revealed the presence of diverse viral communities in wild and cultivated Poaceae, even though they did not present any symptoms. These viruses belong to diverse families (e.g. Endornaviridae, Luteoviridae, Partitiviridae, Potyviridae, Reoviridae, Secoviridae), infecting a large range of hosts within the Poaceae and transmitted by different vectors (aphids, planthoppers, mites, nematods) or seed-borne. Interestingly, minor Poaceae species studied in grasslands contained a wide virus richness, sometimes greater than ecosystemic pools containing 8-11 major plant species. Moreover, some virus genera (e.g. Amalgavirus, Alphaendornavirus, Potyvirus, Sobemovirus) were found only in minor species, underlining the importance of minor plant species as virus reservoirs in wild ecosystems such as grasslands

Filloux Denis - One of the best experts on this subject based on the ideXlab platform.

  • Identification, molecular and biological characterization of two novel secovirids in wild grass species in Belgium
    2021
    Co-Authors: Maclot François, Filloux Denis, Debue Virginie, Blouin Arnaud, Fontdevila Pareta Núria, Tamisier Lucie, Massart Sébastien
    Abstract:

    High throughput sequencing was performed on virion-associated nucleic acids (VANA) from a pool of fifty asymptomatic rough bluegrasses (Poa trivialis L.) collected in a Belgian grazed pasture. Bioinformatics analyses produced some contigs presenting similarities with secovirid genomes, in particular nepoviruses and waikaviruses. Three distinct positive-sense single-stranded RNAs including 5’ and 3’ UTR were reconstructed and they represented two novel viruses infecting rough bluegrass, for which the provisional names Poaceae Liege nepovirus A (PoLNVA, 7298 nt for RNA1 and 4263 nt for RNA2) and Poaceae Liege virus 1 (PoLV1, 11,623 nt) were proposed. Compared to other Secoviridae members, the highest amino acid identity reached 90.7 % and 66.7 % between PoLNVA and nepoviruses for the Pro-Pol and CP regions respectively, while PoLV1 presented the highest amino acid identity with waikaviruses but with lower identities, i.e. 41.2 % for Pro-Pol and 25.8 % for CP regions, far below the ICTV demarcation criteria for novel secovirid. Based on sequence identity and phylogenetic analyses, PoLNVA was proposed to belong to the genus Nepovirus and PoLV1 as an unclassified secovirids. Detection of the two novel viruses was confirmed in high prevalence in rough bluegrass and ten other wild Poaceae species (Agropyron repens, Agrostis capillaris, Apera spica-venti, Anthoxanthum odoratum, Cynosorus cristatus, Festuca rubra, Holcus lanatus, Lolium perenne, Phleum bertolini and Phleum pratense) by RT-PCR and Sanger sequencing, revealing a diverse host range within Poaceae for these novel secovirids. Seed transmission was evaluated and confirmed for PoLNVA.Peer reviewe

  • Identification, molecular and biological characterization of two novel secovirids in wild grass species in Belgium
    'Elsevier BV', 2021
    Co-Authors: Maclot François, Filloux Denis, Debue Virginie, Blouin Arnaud, Fontdevila Pareta Núria, Tamisier Lucie, Massart Sébastien
    Abstract:

    International audienceHigh throughput sequencing was performed on virion-associated nucleic acids (VANA) from a pool of fifty asymptomatic rough bluegrasses (Poa trivialis L.) collected in a Belgian grazed pasture. Bioinformatics analyses produced some contigs presenting similarities with secovirid genomes, in particular nepoviruses and waikaviruses. Three distinct positive-sense single-stranded RNAs including 5? and 3? UTR were reconstructed and they represented two novel viruses infecting rough bluegrass, for which the provisional names Poaceae Liege nepovirus A (PoLNVA, 7298 nt for RNA1 and 4263 nt for RNA2) and Poaceae Liege virus 1 (PoLV1, 11,623 nt) were proposed. Compared to other Secoviridae members, the highest amino acid identity reached 90.7 % and 66.7 % between PoLNVA and nepoviruses for the Pro-Pol and CP regions respectively, while PoLV1 presented the highest amino acid identity with waikaviruses but with lower identities, i.e. 41.2 % for Pro-Pol and 25.8 % for CP regions, far below the ICTV demarcation criteria for novel secovirid. Based on sequence identity and phylogenetic analyses, PoLNVA was proposed to belong to the genus Nepovirus and PoLV1 as an unclassified secovirids. Detection of the two novel viruses was confirmed in high prevalence in rough bluegrass and ten other wild Poaceae species (Agropyron repens, Agrostis capillaris, Apera spica-venti, Anthoxanthum odoratum, Cynosorus cristatus, Festuca rubra, Holcus lanatus, Lolium perenne, Phleum bertolini and Phleum pratense) by RT-PCR and Sanger sequencing, revealing a diverse host range within Poaceae for these novel secovirids. Seed transmission was evaluated and confirmed for PoLNVA

  • Exploration de la diversité et richesse virale dans des communautés végétales de Poacées contrastées
    2020
    Co-Authors: Maclot François, Filloux Denis, Candresse Thierry, Malmstrom Carolyn, Massart Sébastien
    Abstract:

    audience: researcherPrior to plant domestication, it is hypothesized that plant viruses were co-evolving with uncultivated plants growing in mixed species communities, thereby resulting in complex interactions (antagonism, commensalism, mutualism). Development of agriculture further deeply modified natural ecosystems, land use and dynamics of virus-plant interactions, which has fostered occurrence of virus disease emergence events. In this context, we are conducting a study in the Natural Park “Burdinale-Mehaigne” (Belgium) using high throughput sequencing technologies in order to examine the impact of plant species diversity on the virome (e.g. the genomes of viral community) of Poaceae in contrasted plant communities (cereal monocultures, grazed pastures and natural grasslands). We adapted a virion-associated nucleic acids (VANA) metagenomics protocol to sequence at high throughput pools of 50 plant samples per plant community (50 samples reflecting plant species composition) and per plant species. Over two years, about 4,300 Poaceae plants (corresponding to 24 species) were sampled and bioinformatic analyses revealed presence of diverse viral communities in wild and cultivated Poaceae, even though they did not present any symptoms. These viruses belong to diverse families (e.g. Alphaflexiviridae, Luteoviridae, Partitiviridae, Totiviridae), infecting a large range of hosts within Poaceae and transmitted by different vectors (insects, mites, nematods) or seed-borne. More than 30 virus species were detected in the different plots and at least half of them are candidates for new virus species, revealing that virome in Poaceae-based agroecosystems and its impact on the dynamic of plant communities remain largely unexplored.Impact of ecosystem diversity on Poaceae virom

  • Exploration de la diversité et richesse virale dans des communautés végétales de Poacées contrastées
    2020
    Co-Authors: Maclot François, Filloux Denis, Candresse Thierry, Malmstrom Carolyn, Massart Sébastien
    Abstract:

    Prior to plant domestication, it is hypothesized that plant viruses were co-evolving with uncultivated plants growing in mixed species communities, thereby resulting in complex interactions (antagonism, commensalism, mutualism). Development of agriculture further deeply modified natural ecosystems, land use and dynamics of virus-plant interactions, which has fostered occurrence of virus disease emergence events. In this context, we are conducting a study in the Natural Park “Burdinale-Mehaigne” (Belgium) using high throughput sequencing technologies in order to examine the impact of plant species diversity on the virome (e.g. the genomes of viral community) of Poaceae in contrasted plant communities (cereal monocultures, grazed pastures and natural grasslands). We adapted a virion-associated nucleic acids (VANA) metagenomics protocol to sequence at high throughput pools of 50 plant samples per plant community (50 samples reflecting plant species composition) and per plant species. Over two years, about 4,300 Poaceae plants (corresponding to 24 species) were sampled and bioinformatic analyses revealed presence of diverse viral communities in wild and cultivated Poaceae, even though they did not present any symptoms. These viruses belong to diverse families (e.g. Alphaflexiviridae, Luteoviridae, Partitiviridae, Totiviridae), infecting a large range of hosts within Poaceae and transmitted by different vectors (insects, mites, nematods) or seed-borne. More than 30 virus species were detected in the different plots and at least half of them are candidates for new virus species, revealing that virome in Poaceae-based agroecosystems and its impact on the dynamic of plant communities remain largely unexplored

  • Les espèces végétales minoritaires peuvent jouer un rôle clé dans le virome des Poaceae à l'échelle des agroécosystèmes
    2019
    Co-Authors: Maclot François, Filloux Denis, Roumagnac Philippe, Candresse Thierry, Massart Sébastien
    Abstract:

    Prior to the domestication of plants, it is hypothesized that plant viruses were co-evolving with uncultivated plants growing in mixed species communities, which probably resulted in complex interactions (antagonism, commensalism, mutualism). The development of agriculture further deeply modified natural ecosystems and land use, creating agroecosystems composed by both cultivated and uncultivated areas. It is postulated that the advent of agriculture has modified the dynamics of virus-plant interactions, which has fostered the occurrence of virus disease emergence events. At the level of natural ecosystems, the relationships between plant communities’ diversity and plant virus diversity, the distribution of plant viruses and the interactions between viruses and their uncultivated hosts, have just started to be explored. In this context, we are conducting a study in the Natural Park “Burdinale-Mehaigne” (Belgium) using high throughput sequencing technologies in order to characterize the virome of Poaceae communities, including minor species, in contrasted agricultural ecosystems (cereal monocultures, grazed pastures and natural grasslands). We adapted a virion-associated nucleic acids (VANA) metagenomics protocol to sequence at high throughput pools of 50 plant samples per ecosystem (50 samples reflecting plant species composition) and per plant species. Over two years, about 4,300 Poaceae plants (corresponding to 24 species) were sampled and processed using the VANA metagenomics approach. The bioinformatic analyses revealed the presence of diverse viral communities in wild and cultivated Poaceae, even though they did not present any symptoms. These viruses belong to diverse families (e.g. Endornaviridae, Luteoviridae, Partitiviridae, Potyviridae, Reoviridae, Secoviridae), infecting a large range of hosts within the Poaceae and transmitted by different vectors (aphids, planthoppers, mites, nematods) or seed-borne. Interestingly, minor Poaceae species studied in grasslands contained a wide virus richness, sometimes greater than ecosystemic pools containing 8-11 major plant species. Moreover, some virus genera (e.g. Amalgavirus, Alphaendornavirus, Potyvirus, Sobemovirus) were found only in minor species, underlining the importance of minor plant species as virus reservoirs in wild ecosystems such as grasslands

Candresse Thierry - One of the best experts on this subject based on the ideXlab platform.

  • Exploration de la diversité et richesse virale dans des communautés végétales de Poacées contrastées
    2020
    Co-Authors: Maclot François, Filloux Denis, Candresse Thierry, Malmstrom Carolyn, Massart Sébastien
    Abstract:

    audience: researcherPrior to plant domestication, it is hypothesized that plant viruses were co-evolving with uncultivated plants growing in mixed species communities, thereby resulting in complex interactions (antagonism, commensalism, mutualism). Development of agriculture further deeply modified natural ecosystems, land use and dynamics of virus-plant interactions, which has fostered occurrence of virus disease emergence events. In this context, we are conducting a study in the Natural Park “Burdinale-Mehaigne” (Belgium) using high throughput sequencing technologies in order to examine the impact of plant species diversity on the virome (e.g. the genomes of viral community) of Poaceae in contrasted plant communities (cereal monocultures, grazed pastures and natural grasslands). We adapted a virion-associated nucleic acids (VANA) metagenomics protocol to sequence at high throughput pools of 50 plant samples per plant community (50 samples reflecting plant species composition) and per plant species. Over two years, about 4,300 Poaceae plants (corresponding to 24 species) were sampled and bioinformatic analyses revealed presence of diverse viral communities in wild and cultivated Poaceae, even though they did not present any symptoms. These viruses belong to diverse families (e.g. Alphaflexiviridae, Luteoviridae, Partitiviridae, Totiviridae), infecting a large range of hosts within Poaceae and transmitted by different vectors (insects, mites, nematods) or seed-borne. More than 30 virus species were detected in the different plots and at least half of them are candidates for new virus species, revealing that virome in Poaceae-based agroecosystems and its impact on the dynamic of plant communities remain largely unexplored.Impact of ecosystem diversity on Poaceae virom

  • Exploration de la diversité et richesse virale dans des communautés végétales de Poacées contrastées
    2020
    Co-Authors: Maclot François, Filloux Denis, Candresse Thierry, Malmstrom Carolyn, Massart Sébastien
    Abstract:

    Prior to plant domestication, it is hypothesized that plant viruses were co-evolving with uncultivated plants growing in mixed species communities, thereby resulting in complex interactions (antagonism, commensalism, mutualism). Development of agriculture further deeply modified natural ecosystems, land use and dynamics of virus-plant interactions, which has fostered occurrence of virus disease emergence events. In this context, we are conducting a study in the Natural Park “Burdinale-Mehaigne” (Belgium) using high throughput sequencing technologies in order to examine the impact of plant species diversity on the virome (e.g. the genomes of viral community) of Poaceae in contrasted plant communities (cereal monocultures, grazed pastures and natural grasslands). We adapted a virion-associated nucleic acids (VANA) metagenomics protocol to sequence at high throughput pools of 50 plant samples per plant community (50 samples reflecting plant species composition) and per plant species. Over two years, about 4,300 Poaceae plants (corresponding to 24 species) were sampled and bioinformatic analyses revealed presence of diverse viral communities in wild and cultivated Poaceae, even though they did not present any symptoms. These viruses belong to diverse families (e.g. Alphaflexiviridae, Luteoviridae, Partitiviridae, Totiviridae), infecting a large range of hosts within Poaceae and transmitted by different vectors (insects, mites, nematods) or seed-borne. More than 30 virus species were detected in the different plots and at least half of them are candidates for new virus species, revealing that virome in Poaceae-based agroecosystems and its impact on the dynamic of plant communities remain largely unexplored

  • Les espèces végétales minoritaires peuvent jouer un rôle clé dans le virome des Poaceae à l'échelle des agroécosystèmes
    2019
    Co-Authors: Maclot François, Filloux Denis, Roumagnac Philippe, Candresse Thierry, Massart Sébastien
    Abstract:

    Prior to the domestication of plants, it is hypothesized that plant viruses were co-evolving with uncultivated plants growing in mixed species communities, which probably resulted in complex interactions (antagonism, commensalism, mutualism). The development of agriculture further deeply modified natural ecosystems and land use, creating agroecosystems composed by both cultivated and uncultivated areas. It is postulated that the advent of agriculture has modified the dynamics of virus-plant interactions, which has fostered the occurrence of virus disease emergence events. At the level of natural ecosystems, the relationships between plant communities’ diversity and plant virus diversity, the distribution of plant viruses and the interactions between viruses and their uncultivated hosts, have just started to be explored. In this context, we are conducting a study in the Natural Park “Burdinale-Mehaigne” (Belgium) using high throughput sequencing technologies in order to characterize the virome of Poaceae communities, including minor species, in contrasted agricultural ecosystems (cereal monocultures, grazed pastures and natural grasslands). We adapted a virion-associated nucleic acids (VANA) metagenomics protocol to sequence at high throughput pools of 50 plant samples per ecosystem (50 samples reflecting plant species composition) and per plant species. Over two years, about 4,300 Poaceae plants (corresponding to 24 species) were sampled and processed using the VANA metagenomics approach. The bioinformatic analyses revealed the presence of diverse viral communities in wild and cultivated Poaceae, even though they did not present any symptoms. These viruses belong to diverse families (e.g. Endornaviridae, Luteoviridae, Partitiviridae, Potyviridae, Reoviridae, Secoviridae), infecting a large range of hosts within the Poaceae and transmitted by different vectors (aphids, planthoppers, mites, nematods) or seed-borne. Interestingly, minor Poaceae species studied in grasslands contained a wide virus richness, sometimes greater than ecosystemic pools containing 8-11 major plant species. Moreover, some virus genera (e.g. Amalgavirus, Alphaendornavirus, Potyvirus, Sobemovirus) were found only in minor species, underlining the importance of minor plant species as virus reservoirs in wild ecosystems such as grasslands

  • Les espèces végétales minoritaires peuvent jouer un rôle clé dans le virome des Poaceae à l'échelle des agroécosystèmes
    2019
    Co-Authors: Maclot François, Filloux Denis, Roumagnac Philippe, Candresse Thierry, Massart Sébastien
    Abstract:

    audience: researcherPrior to the domestication of plants, it is hypothesized that plant viruses were co-evolving with uncultivated plants growing in mixed species communities, which probably resulted in complex interactions (antagonism, commensalism, mutualism). The development of agriculture further deeply modified natural ecosystems and land use, creating agroecosystems composed by both cultivated and uncultivated areas. It is postulated that the advent of agriculture has modified the dynamics of virus-plant interactions, which has fostered the occurrence of virus disease emergence events. At the level of natural ecosystems, the relationships between plant communities’ diversity and plant virus diversity, the distribution of plant viruses and the interactions between viruses and their uncultivated hosts, have just started to be explored. In this context, we are conducting a study in the Natural Park “Burdinale-Mehaigne” (Belgium) using high throughput sequencing technologies in order to characterize the virome of Poaceae communities, including minor species, in contrasted agricultural ecosystems (cereal monocultures, grazed pastures and natural grasslands). We adapted a virion-associated nucleic acids (VANA) metagenomics protocol to sequence at high throughput pools of 50 plant samples per ecosystem (50 samples reflecting plant species composition) and per plant species. Over two years, about 4,300 Poaceae plants (corresponding to 24 species) were sampled and processed using the VANA metagenomics approach. The bioinformatic analyses revealed the presence of diverse viral communities in wild and cultivated Poaceae, even though they did not present any symptoms. These viruses belong to diverse families (e.g. Endornaviridae, Luteoviridae, Partitiviridae, Potyviridae, Reoviridae, Secoviridae), infecting a large range of hosts within the Poaceae and transmitted by different vectors (aphids, planthoppers, mites, nematods) or seed-borne. Interestingly, minor Poaceae species studied in grasslands contained a wide virus richness, sometimes greater than ecosystemic pools containing 8-11 major plant species. Moreover, some virus genera (e.g. Amalgavirus, Alphaendornavirus, Potyvirus, Sobemovirus) were found only in minor species, underlining the importance of minor plant species as virus reservoirs in wild ecosystems such as grasslands

  • Exploring the virus richness and diversity in contrasted plant communities of Poaceae
    2019
    Co-Authors: Maclot François, Filloux Denis, Roumagnac Philippe, Candresse Thierry, Malmstrom Carolyn, Massart Sébastien
    Abstract:

    audience: researcherPrior to the domestication of plants, it is hypothesized that plant viruses were only co-evolving with uncultivated plants growing in mixed species communities, thereby resulting in complex interactions (antagonism, commensalism, mutualism). The development of agriculture further deeply modified natural ecosystems and land use, creating agroecosystems composed by both cultivated and uncultivated areas. It is postulated that the advent of agriculture has modified the dynamics of virus-plant interactions, which has fostered the occurrence of virus disease emergence events. In this context, we are conducting a study in the Natural Park “Burdinale-Mehaigne” (Belgium) using high throughput sequencing technologies in order to examine the impact of species diversity on the virome of Poaceae communities in contrasted agricultural ecosystems (cereal monocultures, grazed pastures and natural grasslands). We adapted a virion-associated nucleic acids (VANA) metagenomics protocol to sequence at high throughput pools of 50 plant samples per ecosystem (50 samples reflecting plant species composition) and per plant species. Over two years, about 4,300 Poaceae plants (corresponding to 24 species) were sampled and processed using the VANA metagenomics approach. The bioinformatic analyses revealed the presence of diverse viral communities in wild and cultivated Poaceae, even though they did not present any symptoms. These viruses belong to diverse families (e.g. Alphaflexiviridae, Endornaviridae, Luteoviridae, Partitiviridae, Potyviridae, Reoviridae, Secoviridae), infecting a large range of hosts within the Poaceae and transmitted by different vectors (aphids, planthoppers, mites, nematods) or seed-borne. Interestingly, mycoviruses (Chrysovirus and Totivirus genera) were found in all ecosystems, in particular wheat and barley fields. Out of the 23 virus species detected in the different plots, ten of them are condidates for new virus species, revealing that virome in Poaceae-based agroecosystems remains unexplored. To confirm these results and determine the virus incidence in the different ecosystems, total RNA extraction and RT-PCR were implemented on 600 individual plants, targeting five viruses: three viruses detected in several ecosystems (Barley yellow dwarf virus, Lolium latent virus, Ryegrass mosaic virus) and two candidates for new virus species (belonging to Nepovirus and Waikavirus genera). The preliminary results are presented here.Impact of ecosystem diversity on Poaceae virom

Roumagnac Philippe - One of the best experts on this subject based on the ideXlab platform.

  • Les espèces végétales minoritaires peuvent jouer un rôle clé dans le virome des Poaceae à l'échelle des agroécosystèmes
    2019
    Co-Authors: Maclot François, Filloux Denis, Roumagnac Philippe, Candresse Thierry, Massart Sébastien
    Abstract:

    Prior to the domestication of plants, it is hypothesized that plant viruses were co-evolving with uncultivated plants growing in mixed species communities, which probably resulted in complex interactions (antagonism, commensalism, mutualism). The development of agriculture further deeply modified natural ecosystems and land use, creating agroecosystems composed by both cultivated and uncultivated areas. It is postulated that the advent of agriculture has modified the dynamics of virus-plant interactions, which has fostered the occurrence of virus disease emergence events. At the level of natural ecosystems, the relationships between plant communities’ diversity and plant virus diversity, the distribution of plant viruses and the interactions between viruses and their uncultivated hosts, have just started to be explored. In this context, we are conducting a study in the Natural Park “Burdinale-Mehaigne” (Belgium) using high throughput sequencing technologies in order to characterize the virome of Poaceae communities, including minor species, in contrasted agricultural ecosystems (cereal monocultures, grazed pastures and natural grasslands). We adapted a virion-associated nucleic acids (VANA) metagenomics protocol to sequence at high throughput pools of 50 plant samples per ecosystem (50 samples reflecting plant species composition) and per plant species. Over two years, about 4,300 Poaceae plants (corresponding to 24 species) were sampled and processed using the VANA metagenomics approach. The bioinformatic analyses revealed the presence of diverse viral communities in wild and cultivated Poaceae, even though they did not present any symptoms. These viruses belong to diverse families (e.g. Endornaviridae, Luteoviridae, Partitiviridae, Potyviridae, Reoviridae, Secoviridae), infecting a large range of hosts within the Poaceae and transmitted by different vectors (aphids, planthoppers, mites, nematods) or seed-borne. Interestingly, minor Poaceae species studied in grasslands contained a wide virus richness, sometimes greater than ecosystemic pools containing 8-11 major plant species. Moreover, some virus genera (e.g. Amalgavirus, Alphaendornavirus, Potyvirus, Sobemovirus) were found only in minor species, underlining the importance of minor plant species as virus reservoirs in wild ecosystems such as grasslands

  • Les espèces végétales minoritaires peuvent jouer un rôle clé dans le virome des Poaceae à l'échelle des agroécosystèmes
    2019
    Co-Authors: Maclot François, Filloux Denis, Roumagnac Philippe, Candresse Thierry, Massart Sébastien
    Abstract:

    audience: researcherPrior to the domestication of plants, it is hypothesized that plant viruses were co-evolving with uncultivated plants growing in mixed species communities, which probably resulted in complex interactions (antagonism, commensalism, mutualism). The development of agriculture further deeply modified natural ecosystems and land use, creating agroecosystems composed by both cultivated and uncultivated areas. It is postulated that the advent of agriculture has modified the dynamics of virus-plant interactions, which has fostered the occurrence of virus disease emergence events. At the level of natural ecosystems, the relationships between plant communities’ diversity and plant virus diversity, the distribution of plant viruses and the interactions between viruses and their uncultivated hosts, have just started to be explored. In this context, we are conducting a study in the Natural Park “Burdinale-Mehaigne” (Belgium) using high throughput sequencing technologies in order to characterize the virome of Poaceae communities, including minor species, in contrasted agricultural ecosystems (cereal monocultures, grazed pastures and natural grasslands). We adapted a virion-associated nucleic acids (VANA) metagenomics protocol to sequence at high throughput pools of 50 plant samples per ecosystem (50 samples reflecting plant species composition) and per plant species. Over two years, about 4,300 Poaceae plants (corresponding to 24 species) were sampled and processed using the VANA metagenomics approach. The bioinformatic analyses revealed the presence of diverse viral communities in wild and cultivated Poaceae, even though they did not present any symptoms. These viruses belong to diverse families (e.g. Endornaviridae, Luteoviridae, Partitiviridae, Potyviridae, Reoviridae, Secoviridae), infecting a large range of hosts within the Poaceae and transmitted by different vectors (aphids, planthoppers, mites, nematods) or seed-borne. Interestingly, minor Poaceae species studied in grasslands contained a wide virus richness, sometimes greater than ecosystemic pools containing 8-11 major plant species. Moreover, some virus genera (e.g. Amalgavirus, Alphaendornavirus, Potyvirus, Sobemovirus) were found only in minor species, underlining the importance of minor plant species as virus reservoirs in wild ecosystems such as grasslands

  • Exploring the virus richness and diversity in contrasted plant communities of Poaceae
    2019
    Co-Authors: Maclot François, Filloux Denis, Roumagnac Philippe, Candresse Thierry, Malmstrom Carolyn, Massart Sébastien
    Abstract:

    audience: researcherPrior to the domestication of plants, it is hypothesized that plant viruses were only co-evolving with uncultivated plants growing in mixed species communities, thereby resulting in complex interactions (antagonism, commensalism, mutualism). The development of agriculture further deeply modified natural ecosystems and land use, creating agroecosystems composed by both cultivated and uncultivated areas. It is postulated that the advent of agriculture has modified the dynamics of virus-plant interactions, which has fostered the occurrence of virus disease emergence events. In this context, we are conducting a study in the Natural Park “Burdinale-Mehaigne” (Belgium) using high throughput sequencing technologies in order to examine the impact of species diversity on the virome of Poaceae communities in contrasted agricultural ecosystems (cereal monocultures, grazed pastures and natural grasslands). We adapted a virion-associated nucleic acids (VANA) metagenomics protocol to sequence at high throughput pools of 50 plant samples per ecosystem (50 samples reflecting plant species composition) and per plant species. Over two years, about 4,300 Poaceae plants (corresponding to 24 species) were sampled and processed using the VANA metagenomics approach. The bioinformatic analyses revealed the presence of diverse viral communities in wild and cultivated Poaceae, even though they did not present any symptoms. These viruses belong to diverse families (e.g. Alphaflexiviridae, Endornaviridae, Luteoviridae, Partitiviridae, Potyviridae, Reoviridae, Secoviridae), infecting a large range of hosts within the Poaceae and transmitted by different vectors (aphids, planthoppers, mites, nematods) or seed-borne. Interestingly, mycoviruses (Chrysovirus and Totivirus genera) were found in all ecosystems, in particular wheat and barley fields. Out of the 23 virus species detected in the different plots, ten of them are condidates for new virus species, revealing that virome in Poaceae-based agroecosystems remains unexplored. To confirm these results and determine the virus incidence in the different ecosystems, total RNA extraction and RT-PCR were implemented on 600 individual plants, targeting five viruses: three viruses detected in several ecosystems (Barley yellow dwarf virus, Lolium latent virus, Ryegrass mosaic virus) and two candidates for new virus species (belonging to Nepovirus and Waikavirus genera). The preliminary results are presented here.Impact of ecosystem diversity on Poaceae virom

  • Exploring the virus richness and diversity in contrasted plant communities of Poaceae
    2019
    Co-Authors: Maclot François, Filloux Denis, Roumagnac Philippe, Candresse Thierry, Malmstrom Carolyn, Massart Sébastien
    Abstract:

    Prior to the domestication of plants, it is hypothesized that plant viruses were only co-evolving with uncultivated plants growing in mixed species communities, thereby resulting in complex interactions (antagonism, commensalism, mutualism). The development of agriculture further deeply modified natural ecosystems and land use, creating agroecosystems composed by both cultivated and uncultivated areas. It is postulated that the advent of agriculture has modified the dynamics of virus-plant interactions, which has fostered the occurrence of virus disease emergence events. In this context, we are conducting a study in the Natural Park “Burdinale-Mehaigne” (Belgium) using high throughput sequencing technologies in order to examine the impact of species diversity on the virome of Poaceae communities in contrasted agricultural ecosystems (cereal monocultures, grazed pastures and natural grasslands). We adapted a virion-associated nucleic acids (VANA) metagenomics protocol to sequence at high throughput pools of 50 plant samples per ecosystem (50 samples reflecting plant species composition) and per plant species. Over two years, about 4,300 Poaceae plants (corresponding to 24 species) were sampled and processed using the VANA metagenomics approach. The bioinformatic analyses revealed the presence of diverse viral communities in wild and cultivated Poaceae, even though they did not present any symptoms. These viruses belong to diverse families (e.g. Alphaflexiviridae, Endornaviridae, Luteoviridae, Partitiviridae, Potyviridae, Reoviridae, Secoviridae), infecting a large range of hosts within the Poaceae and transmitted by different vectors (aphids, planthoppers, mites, nematods) or seed-borne. Interestingly, mycoviruses (Chrysovirus and Totivirus genera) were found in all ecosystems, in particular wheat and barley fields. Out of the 23 virus species detected in the different plots, ten of them are condidates for new virus species, revealing that virome in Poaceae-based agroecosystems remains unexplored. To confirm these results and determine the virus incidence in the different ecosystems, total RNA extraction and RT-PCR were implemented on 600 individual plants, targeting five viruses: three viruses detected in several ecosystems (Barley yellow dwarf virus, Lolium latent virus, Ryegrass mosaic virus) and two candidates for new virus species (belonging to Nepovirus and Waikavirus genera). The preliminary results are presented here

  • Impact de la diversité des écosystèmes sur le virome des Poaceae
    2018
    Co-Authors: Maclot François, Filloux Denis, Roumagnac Philippe, Candresse Thierry, Massart Sébastien
    Abstract:

    audience: researcherThis STSM was dedicated to the study of the viral diversity of different agro-ecological systems by metagenomic analyses. Indeed, before the domestication of plants, it is hypothesized that plant viruses were co-evolving with wild plants growing in mixed species communities, thereby resulting in complex interactions. The development of agriculture deeply modified ecosystems, which is postulated to have altered the dynamics of virus-plant pathosystems and accelerated the rate of virus evolution and emergence. In this context, the objective of my PhD is to study the impact of ecosystems with contrasted biodiversity (monoculture, pastures and grasslands of the Natural Park “Burdinale-Mehaigne”, Belgium) on the virome of Poaceae plant species using high throughput sequencing technologies. We developed a metagenomic approach consisting in virus particles purification, virion-associated nucleic acids (VANA) extraction and library preparation for Illumina sequencing applied on pools of 50 plant samples per ecosystem. Bringing my data with me, I visited for one month (March 2018) the team of Professor Philippe Roumagnac (MicroQuar, UMR BGPI, CIRAD, Montpellier, France) in order to discuss the statistical approaches used to characterize viral dynamics in ecosystems and to develop knowledge in the bioinformatic tools adapted to virus detection. The bioIT analysis of my VANA samples revealed the presence of viral communities in wild and cultivated Poaceae. These viruses belong to a quite diverse set of families (e.g. Endornaviridae, Luteoviridae, Partitiviridae, Potyviridae, Reoviridae, Secoviridae), infecting a large host range within the Poaceae and transmitted by various vectors (aphids, planthoppers, mites, nematods, seeds). This STSM had a very positive impact on (1) my work by allowing me to develop my own bioIT pipeline which is still used and optimized in the framework of my ongoing analyses, and (2) my lab with whom a new collaboration was initiated with the MicroQuar team. The preliminary results obtained will be presented.Ecosystem diversity and Poaceae Virom