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J I Hormaza - One of the best experts on this subject based on the ideXlab platform.

  • Effect of temperature on Pollen Germination and Pollen tube growth in longan (Dimocarpus longan Lour.)
    Scientia Horticulturae, 2015
    Co-Authors: V.t. Pham, Maria Herrero, J I Hormaza
    Abstract:

    ABSTRACT Longan (Dimocarpus longan) is a subtropical fruit tree with a long cultivation tradition in South East Asia, and also with a clear potential in other areas of the world. In other plant species, the reproductive phase is most vulnerable to temperature conditions, but information on the effect of temperature on Pollen Germination and tube growth in longan is still lacking. In order to fill this gap, in this work the effect of temperature on longan Pollen Germination was assessed both in vivo and in vitro. For this purpose first Pollen Germination was evaluated in vivo at three temperature regimes, showing the best performance at 23/24 °C. Then an in vitro Pollen Germination medium was optimized for longan and Pollen storage conditions were evaluated, showing good Pollen Germination in vitro after storage for one year at −20 °C. Finally, the effect of temperature was evaluated in vitro in four cultivars and for two male flower types M1 and M2, differing on the degree of pistil differentiation. Both Pollen types behave similarly in all conditions, and all the cultivars studied had good Pollen Germination in vitro at 30 °C, a temperature higher than that reported as optimum in vivo reflecting a higher sensitivity of the stigma than Pollen to high temperatures. However, clear differences between cultivars in their performance at 20 °C and 40 °C were recorded. These results set the basis for reproductive biology studies and breeding in longan, opening a window to select the best cultivars for different temperature conditions.

  • in vitro Pollen Germination in avocado persea americana mill optimization of the method and effect of temperature
    Scientia Horticulturae, 2011
    Co-Authors: M L Alcaraz, Marta Montserrat, J I Hormaza
    Abstract:

    Abstract An improved in vitro Pollen Germination method was developed for avocado ( Persea americana Mill.). The effect of different concentrations of sucrose, polyethylene glycol (PEG), Mg and Ca on Pollen Germination was evaluated in order to determine the optimal Pollen Germination medium, i.e. that maximizing the percentage of Pollen Germination and minimizing the percentage of bursted Pollen grains. Once the Germination medium was optimized we used it to study the effect of temperature on in vitro Pollen Germination and tube growth in different cultivars from the three botanical varieties of avocado, that differ in their adaptation to environmental conditions. Significant differences in percentage of Pollen Germination and in Pollen tube growth were observed among cultivars. These results could have implications not only for optimizing Pollen management in avocado but also to select the best pollinizers for a particular cultivar.

  • In vitro Pollen Germination in avocado (Persea americana Mill.): optimization of the method and effect of temperature
    Scientia Horticulturae, 2011
    Co-Authors: M L Alcaraz, Marta Montserrat, J I Hormaza
    Abstract:

    An improved in vitro Pollen Germination method was developed for avocado (Persea americana Mill.). The effect of different concentrations of sucrose, polyethylene glycol (PEG), Mg and Ca on Pollen Germination was evaluated in order to determine the optimal Pollen Germination medium, i.e. that maximizing the percentage of Pollen Germination and minimizing the percentage of bursted Pollen grains. Once the Germination medium was optimized we used it to study the effect of temperature on in vitro Pollen Germination and tube growth in different cultivars from the three botanical varieties of avocado, that differ in their adaptation to environmental conditions. Significant differences in percentage of Pollen Germination and in Pollen tube growth were observed among cultivars. These results could have implications not only for optimizing Pollen management in avocado but also to select the best pollinizers for a particular cultivarThe research was supported by the Ministerio de Ciencia e Innovación – European Regional Development Fund, European Union (AGL2010-15140) and Junta de Andalucía (Proyecto de Excelencia P08-AGR-3694).Peer reviewe

Zhangliang Chen - One of the best experts on this subject based on the ideXlab platform.

  • Arabidopsis AtBECLIN 1/AtAtg6/AtVps30 is essential for Pollen Germination and plant development.
    Cell research, 2007
    Co-Authors: Genji Qin, Li Zhang, Shufan Xing, Xianhui Hou, Jie Deng, Jingjing Liu, Zhangliang Chen
    Abstract:

    Pollen Germination on the surface of compatible stigmatic tissues is an essential step for plant fertilization. Here we report that the Arabidopsis mutant bcl1 is male sterile as a result of the failure of Pollen Germination. We show that the bcl1 mutant allele cannot be transmitted by male gametophytes and no homozygous bcl1 mutants were obtained. Analysis of Pollen developmental stages indicates that the bcl1 mutation affects Pollen Germination but not Pollen maturation. Molecular analysis demonstrates that the failure of Pollen Germination was caused by the disruption of AtBECLIN 1. AtBECLIN 1 is expressed predominantly in mature Pollen and encodes a protein with significant homology to Beclin1/Atg6/Vps30 required for the processes of autophagy and vacuolar protein sorting (VPS) in yeast. We also show that AtBECLIN 1 is required for normal plant development, and that genes related to autophagy, VPS and the glycosylphosphatidylinositol anchor system, were affected by the deficiency of AtBECLIN 1.

  • arabidopsis atbeclin 1 atatg6 atvps30 is essential for Pollen Germination and plant development
    Cell Research, 2007
    Co-Authors: Genji Qin, Li Zhang, Shufan Xing, Xianhui Hou, Jie Deng, Jingjing Liu, Zhangliang Chen
    Abstract:

    Pollen Germination on the surface of compatible stigmatic tissues is an essential step for plant fertilization. Here we report that the Arabidopsis mutant bcl1 is male sterile as a result of the failure of Pollen Germination. We show that the bcl1 mutant allele cannot be transmitted by male gametophytes and no homozygous bcl1 mutants were obtained. Analysis of Pollen developmental stages indicates that the bcl1 mutation affects Pollen Germination but not Pollen maturation. Molecular analysis demonstrates that the failure of Pollen Germination was caused by the disruption of AtBECLIN 1. AtBECLIN 1 is expressed predominantly in mature Pollen and encodes a protein with significant homology to Beclin1/Atg6/Vps30 required for the processes of autophagy and vacuolar protein sorting (VPS) in yeast. We also show that AtBECLIN 1 is required for normal plant development, and that genes related to autophagy, VPS and the glycosylphosphatidylinositol anchor system, were affected by the deficiency of AtBECLIN 1.

Genji Qin - One of the best experts on this subject based on the ideXlab platform.

  • Arabidopsis AtBECLIN 1/AtAtg6/AtVps30 is essential for Pollen Germination and plant development.
    Cell research, 2007
    Co-Authors: Genji Qin, Li Zhang, Shufan Xing, Xianhui Hou, Jie Deng, Jingjing Liu, Zhangliang Chen
    Abstract:

    Pollen Germination on the surface of compatible stigmatic tissues is an essential step for plant fertilization. Here we report that the Arabidopsis mutant bcl1 is male sterile as a result of the failure of Pollen Germination. We show that the bcl1 mutant allele cannot be transmitted by male gametophytes and no homozygous bcl1 mutants were obtained. Analysis of Pollen developmental stages indicates that the bcl1 mutation affects Pollen Germination but not Pollen maturation. Molecular analysis demonstrates that the failure of Pollen Germination was caused by the disruption of AtBECLIN 1. AtBECLIN 1 is expressed predominantly in mature Pollen and encodes a protein with significant homology to Beclin1/Atg6/Vps30 required for the processes of autophagy and vacuolar protein sorting (VPS) in yeast. We also show that AtBECLIN 1 is required for normal plant development, and that genes related to autophagy, VPS and the glycosylphosphatidylinositol anchor system, were affected by the deficiency of AtBECLIN 1.

  • arabidopsis atbeclin 1 atatg6 atvps30 is essential for Pollen Germination and plant development
    Cell Research, 2007
    Co-Authors: Genji Qin, Li Zhang, Shufan Xing, Xianhui Hou, Jie Deng, Jingjing Liu, Zhangliang Chen
    Abstract:

    Pollen Germination on the surface of compatible stigmatic tissues is an essential step for plant fertilization. Here we report that the Arabidopsis mutant bcl1 is male sterile as a result of the failure of Pollen Germination. We show that the bcl1 mutant allele cannot be transmitted by male gametophytes and no homozygous bcl1 mutants were obtained. Analysis of Pollen developmental stages indicates that the bcl1 mutation affects Pollen Germination but not Pollen maturation. Molecular analysis demonstrates that the failure of Pollen Germination was caused by the disruption of AtBECLIN 1. AtBECLIN 1 is expressed predominantly in mature Pollen and encodes a protein with significant homology to Beclin1/Atg6/Vps30 required for the processes of autophagy and vacuolar protein sorting (VPS) in yeast. We also show that AtBECLIN 1 is required for normal plant development, and that genes related to autophagy, VPS and the glycosylphosphatidylinositol anchor system, were affected by the deficiency of AtBECLIN 1.

Hazel Y. Wetzstein - One of the best experts on this subject based on the ideXlab platform.

  • Pollen Germination, tube growth and morphology, and microtubule organization after exposure to benomyl
    Physiologia Plantarum, 1996
    Co-Authors: Barry A. Palevitz, Hazel Y. Wetzstein
    Abstract:

    The inhibitory effects of benomyl on Pollen tube growth have received little attention, particularly at the microscopic and immunohistochemical levels. Pollen Germination and tube growth in the presence of benomyl were evaluated in Tradescantia virginiana to investigate the effects of this fungicide on Pollen Germination rate, tube growth and morphology, and microtubule (Mt) organization. Benomyl was incorporated in Germination media at 0, 480, 600 or 720 mg l -1 . Inhibition of Pollen Germination, cytoplasmic streaming, and tube elongation were associated with benomyl treatments. Benomyl also induced abnormal Pollen tube morphology and Mt organization. Compared to controls, Mts in the treated tubes were characteristically fewer in number, fragmented, sinuous and increasingly disorganized. At the two highest benomyl concentrations, Mts were considerably fewer or absent in apical/subapical regions of the Pollen tubes. This work verifies that benomyl incorporated into Germination media at concentrations lower than recommended field rates inhibit Pollen Germination and tube growth, and that the effects are associated with alterations of Mt organization.

M L Alcaraz - One of the best experts on this subject based on the ideXlab platform.

  • in vitro Pollen Germination in avocado persea americana mill optimization of the method and effect of temperature
    Scientia Horticulturae, 2011
    Co-Authors: M L Alcaraz, Marta Montserrat, J I Hormaza
    Abstract:

    Abstract An improved in vitro Pollen Germination method was developed for avocado ( Persea americana Mill.). The effect of different concentrations of sucrose, polyethylene glycol (PEG), Mg and Ca on Pollen Germination was evaluated in order to determine the optimal Pollen Germination medium, i.e. that maximizing the percentage of Pollen Germination and minimizing the percentage of bursted Pollen grains. Once the Germination medium was optimized we used it to study the effect of temperature on in vitro Pollen Germination and tube growth in different cultivars from the three botanical varieties of avocado, that differ in their adaptation to environmental conditions. Significant differences in percentage of Pollen Germination and in Pollen tube growth were observed among cultivars. These results could have implications not only for optimizing Pollen management in avocado but also to select the best pollinizers for a particular cultivar.

  • In vitro Pollen Germination in avocado (Persea americana Mill.): optimization of the method and effect of temperature
    Scientia Horticulturae, 2011
    Co-Authors: M L Alcaraz, Marta Montserrat, J I Hormaza
    Abstract:

    An improved in vitro Pollen Germination method was developed for avocado (Persea americana Mill.). The effect of different concentrations of sucrose, polyethylene glycol (PEG), Mg and Ca on Pollen Germination was evaluated in order to determine the optimal Pollen Germination medium, i.e. that maximizing the percentage of Pollen Germination and minimizing the percentage of bursted Pollen grains. Once the Germination medium was optimized we used it to study the effect of temperature on in vitro Pollen Germination and tube growth in different cultivars from the three botanical varieties of avocado, that differ in their adaptation to environmental conditions. Significant differences in percentage of Pollen Germination and in Pollen tube growth were observed among cultivars. These results could have implications not only for optimizing Pollen management in avocado but also to select the best pollinizers for a particular cultivarThe research was supported by the Ministerio de Ciencia e Innovación – European Regional Development Fund, European Union (AGL2010-15140) and Junta de Andalucía (Proyecto de Excelencia P08-AGR-3694).Peer reviewe