The Experts below are selected from a list of 46008 Experts worldwide ranked by ideXlab platform
Yue Mao-xing - One of the best experts on this subject based on the ideXlab platform.
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development of a Polyclonal Antibody based enzyme linked immunosorbent assay elisa for detection of sunset yellow fcf in food samples
Talanta, 2012Co-Authors: Yue Mao-xing, Taichang Zhang, Meng Meng, Rimo XiAbstract:Abstract Sunset Yellow FCF is widely used as food additives to make foods more attractive. Due to its abuse and potential risk to human health, Sunset Yellow FCF is precisely limited to use in food. To monitor the illegal use of Sunset Yellow FCF, a Polyclonal Antibody-based indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) with satisfactory sensitivity and specificity was developed. A carboxyl group was introduced to Sunset Yellow FCF, then the modified hapten was coupled with carrier proteins to synthesize the immunogen and coating antigen. The IC50 value of 0.52 ng mL−1 and detection limit of 25 pg mL−1 (in buffer) were achieved by this method. The cross-reactivity values of the antibodies with six structurally related colorants were less than 1.5%, indicating the high selectivity. Three kinds of food samples (beverage, dried beancurd, braised pork) and serum were chosen to evaluate the application of the immunoassay in real systems. The limits of detection (LOD) in the above three food samples were 0.12, 0.04 and 1.11, respectively (mean+3SD). The recovery (94%–106%), intra-assay (
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development of a Polyclonal Antibody based enzyme linked immunosorbent assay elisa for detection of sunset yellow fcf in food samples
Talanta, 2012Co-Authors: Yue Mao-xing, Taichang Zhang, Meng Meng, Huyin Xue, Yongmei YinAbstract:Sunset Yellow FCF is widely used as food additives to make foods more attractive. Due to its abuse and potential risk to human health, Sunset Yellow FCF is precisely limited to use in food. To monitor the illegal use of Sunset Yellow FCF, a Polyclonal Antibody-based indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) with satisfactory sensitivity and specificity was developed. A carboxyl group was introduced to Sunset Yellow FCF, then the modified hapten was coupled with carrier proteins to synthesize the immunogen and coating antigen. The IC(50) value of 0.52 ng mL(-1) and detection limit of 25 pg mL(-1) (in buffer) were achieved by this method. The cross-reactivity values of the antibodies with six structurally related colorants were less than 1.5%, indicating the high selectivity. Three kinds of food samples (beverage, dried beancurd, braised pork) and serum were chosen to evaluate the application of the immunoassay in real systems. The limits of detection (LOD) in the above three food samples were 0.12, 0.04 and 1.11, respectively (mean+3SD). The recovery (94%-106%), intra-assay (<5%) and inter-assay (<12%) coefficients of variation in foods and serum samples were also acceptable. The results suggest that this ELISA method is a specific, sensitive and simple method for the determination of Sunset Yellow FCF additives.
Huisheng Zhuang - One of the best experts on this subject based on the ideXlab platform.
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development of a highly sensitive biotin streptavidin enzyme linked immunosorbent assay for detecting diethyl phthalate based on a specific Polyclonal Antibody
Food and Agricultural Immunology, 2015Co-Authors: Ruiyan Sun, Huisheng ZhuangAbstract:An indirect competitive biotin–streptavidin enzyme-linked immunosorbent assay (BA-ELISA) has been established for the determination of diethyl phthalate (DEP) in wine samples. A highly sensitive and specific Polyclonal Antibody (pAb-DEP) targeting DEP was prepared. Under optimized conditions, good linearity was achieved within a range of 0.021–9.512 μg·L−1. The limit of detection (IC10) was 0.0079 μg·L−1 and the median inhibitory concentration (IC50) was 0.443 μg·L−1. Besides, the BA-ELISA was highly selective, with low cross-reactivity values with DEP analogs (below 5%). Finally, the concentrations of DEP in wine samples ranged from 5.93 μg·L−1 to 59.15 μg·L−1 by BA-ELISA. Satisfactory recoveries (89.19–112.33%) and variation coefficient values (5.81–9.43%) were successfully obtained. The consistency between the results of BA-ELISA and gas chromatography–mass spectrometry (GC–MS) was 97.48%, which further confirmed that the proposed BA-ELISA immunoassay is reliable, rapid, sensitive, and accurate for mon...
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biotin streptavidin enzyme linked immunosorbent assay for the determination of dibutyl phthalate in beverages and drinking water using a specific Polyclonal Antibody
Food Analytical Methods, 2015Co-Authors: Ruiyan Sun, Huisheng ZhuangAbstract:A specific Polyclonal Antibody targeting dibutyl phthalate (DBP) was obtained, and a sensitive indirect competitive biotin-streptavidin enzyme-linked immunosorbent assay (BA-ELISA) was developed for the determination of DBP in beverages and drinking water. Under optimal conditions, good linearity was achieved within a range of 0.02 to 8.97 μg · L−1. The limit of detection (IC10) was 5 ng · L−1, and the median inhibitory concentration (IC50) was 0.36 μg · L−1. The BA-ELISA was highly selective, with lower cross-reactivity values with DBP analogues (lower than 4 %). Finally, the concentrations of DBP in beverages and drinking water by this method ranged from 0.45 to 7.06 μg · L−1. Satisfactory recoveries (89.5 to 109.5 %) and variation coefficient values (6.0 to 8.7 %) were obtained. The consistency between the results obtained from BA-ELISA and gas chromatography-mass spectrometry (GC-MS) was 98.8 %, which further confirmed that this proposed BA-ELISA immunoassay was reliable, rapid, sensitive, and accurate for monitoring DBP in the environmental samples.
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a sensitive heterogeneous biotin streptavidin enzyme linked immunosorbent assay for the determination of di 2 ethylhexyl phthalate dehp in beverages using a specific Polyclonal Antibody
Analytical Methods, 2014Co-Authors: Ruiyan Sun, Huisheng ZhuangAbstract:Di-(2-ethylhexyl)phthalate (DEHP) is one of the long-chain or high-molecular-weight compounds of the phthalic acid diester (PAE) family, which is the most commonly used plasticizer and additive. However, DEHP may cause birth defects, sexual dysfunction, even cancers, possibly heart disease, etc. In order to detect DEHP with high sensitivity and specificity, an indirect competitive biotin–streptavidin enzyme-linked immunosorbent assay (BA-ELISA) has been established in this study. A specific Polyclonal Antibody (pAb-DEHP) targeting DEHP was obtained first, and the procedures of BA-ELISA were optimized for the determination of DEHP in beverages. Under optimal conditions, a good linearity was achieved within a range of 0.021 to 12.948 μg L−1. The limit of detection (IC10) was 0.0074 μg L−1 and the median inhibitory concentration (IC50) was 0.526 μg L−1. The BA-ELISA was highly selective, with low cross-reactivity values with DEHP analogues (lower than 7%). Finally, the assay was successfully used to detect DEHP in beverages; the concentrations of DEHP in the samples ranged from 1.18 μg L−1 to 40.68 μg L−1. Satisfactory recoveries (89.07–109.33%) and coefficient of variation (CV) values (5.97% to 10.68%) were obtained, which further confirmed that this proposed BA-ELISA immunoassay is sensitive, rapid and accurate for monitoring DEHP in the environment.
Rimo Xi - One of the best experts on this subject based on the ideXlab platform.
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development of a Polyclonal Antibody based enzyme linked immunosorbent assay elisa for detection of sunset yellow fcf in food samples
Talanta, 2012Co-Authors: Yue Mao-xing, Taichang Zhang, Meng Meng, Rimo XiAbstract:Abstract Sunset Yellow FCF is widely used as food additives to make foods more attractive. Due to its abuse and potential risk to human health, Sunset Yellow FCF is precisely limited to use in food. To monitor the illegal use of Sunset Yellow FCF, a Polyclonal Antibody-based indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) with satisfactory sensitivity and specificity was developed. A carboxyl group was introduced to Sunset Yellow FCF, then the modified hapten was coupled with carrier proteins to synthesize the immunogen and coating antigen. The IC50 value of 0.52 ng mL−1 and detection limit of 25 pg mL−1 (in buffer) were achieved by this method. The cross-reactivity values of the antibodies with six structurally related colorants were less than 1.5%, indicating the high selectivity. Three kinds of food samples (beverage, dried beancurd, braised pork) and serum were chosen to evaluate the application of the immunoassay in real systems. The limits of detection (LOD) in the above three food samples were 0.12, 0.04 and 1.11, respectively (mean+3SD). The recovery (94%–106%), intra-assay (
Yongmei Yin - One of the best experts on this subject based on the ideXlab platform.
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development of a Polyclonal Antibody based enzyme linked immunosorbent assay elisa for detection of sunset yellow fcf in food samples
Talanta, 2012Co-Authors: Yue Mao-xing, Taichang Zhang, Meng Meng, Huyin Xue, Yongmei YinAbstract:Sunset Yellow FCF is widely used as food additives to make foods more attractive. Due to its abuse and potential risk to human health, Sunset Yellow FCF is precisely limited to use in food. To monitor the illegal use of Sunset Yellow FCF, a Polyclonal Antibody-based indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) with satisfactory sensitivity and specificity was developed. A carboxyl group was introduced to Sunset Yellow FCF, then the modified hapten was coupled with carrier proteins to synthesize the immunogen and coating antigen. The IC(50) value of 0.52 ng mL(-1) and detection limit of 25 pg mL(-1) (in buffer) were achieved by this method. The cross-reactivity values of the antibodies with six structurally related colorants were less than 1.5%, indicating the high selectivity. Three kinds of food samples (beverage, dried beancurd, braised pork) and serum were chosen to evaluate the application of the immunoassay in real systems. The limits of detection (LOD) in the above three food samples were 0.12, 0.04 and 1.11, respectively (mean+3SD). The recovery (94%-106%), intra-assay (<5%) and inter-assay (<12%) coefficients of variation in foods and serum samples were also acceptable. The results suggest that this ELISA method is a specific, sensitive and simple method for the determination of Sunset Yellow FCF additives.
Taichang Zhang - One of the best experts on this subject based on the ideXlab platform.
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development of a Polyclonal Antibody based enzyme linked immunosorbent assay elisa for detection of sunset yellow fcf in food samples
Talanta, 2012Co-Authors: Yue Mao-xing, Taichang Zhang, Meng Meng, Rimo XiAbstract:Abstract Sunset Yellow FCF is widely used as food additives to make foods more attractive. Due to its abuse and potential risk to human health, Sunset Yellow FCF is precisely limited to use in food. To monitor the illegal use of Sunset Yellow FCF, a Polyclonal Antibody-based indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) with satisfactory sensitivity and specificity was developed. A carboxyl group was introduced to Sunset Yellow FCF, then the modified hapten was coupled with carrier proteins to synthesize the immunogen and coating antigen. The IC50 value of 0.52 ng mL−1 and detection limit of 25 pg mL−1 (in buffer) were achieved by this method. The cross-reactivity values of the antibodies with six structurally related colorants were less than 1.5%, indicating the high selectivity. Three kinds of food samples (beverage, dried beancurd, braised pork) and serum were chosen to evaluate the application of the immunoassay in real systems. The limits of detection (LOD) in the above three food samples were 0.12, 0.04 and 1.11, respectively (mean+3SD). The recovery (94%–106%), intra-assay (
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development of a Polyclonal Antibody based enzyme linked immunosorbent assay elisa for detection of sunset yellow fcf in food samples
Talanta, 2012Co-Authors: Yue Mao-xing, Taichang Zhang, Meng Meng, Huyin Xue, Yongmei YinAbstract:Sunset Yellow FCF is widely used as food additives to make foods more attractive. Due to its abuse and potential risk to human health, Sunset Yellow FCF is precisely limited to use in food. To monitor the illegal use of Sunset Yellow FCF, a Polyclonal Antibody-based indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) with satisfactory sensitivity and specificity was developed. A carboxyl group was introduced to Sunset Yellow FCF, then the modified hapten was coupled with carrier proteins to synthesize the immunogen and coating antigen. The IC(50) value of 0.52 ng mL(-1) and detection limit of 25 pg mL(-1) (in buffer) were achieved by this method. The cross-reactivity values of the antibodies with six structurally related colorants were less than 1.5%, indicating the high selectivity. Three kinds of food samples (beverage, dried beancurd, braised pork) and serum were chosen to evaluate the application of the immunoassay in real systems. The limits of detection (LOD) in the above three food samples were 0.12, 0.04 and 1.11, respectively (mean+3SD). The recovery (94%-106%), intra-assay (<5%) and inter-assay (<12%) coefficients of variation in foods and serum samples were also acceptable. The results suggest that this ELISA method is a specific, sensitive and simple method for the determination of Sunset Yellow FCF additives.
