The Experts below are selected from a list of 147 Experts worldwide ranked by ideXlab platform

Wei Shen - One of the best experts on this subject based on the ideXlab platform.

  • red blood cell transport mechanisms in Polyester Thread based blood typing devices
    Analytical and Bioanalytical Chemistry, 2016
    Co-Authors: Azadeh Nilghaz, David Robert Ballerini, Liyun Guan, Lizi Li, Wei Shen
    Abstract:

    A recently developed blood typing diagnostic based on a Polyester Thread substrate has shown great promise for use in medical emergencies and in impoverished regions. The device is easy to use and transport, while also being inexpensive, accurate, and rapid. This study used a fluorescent confocal microscope to delve deeper into how red blood cells were behaving within the Polyester Thread-based diagnostic at the cellular level, and how plasma separation could be made to visibly occur on the Thread, making it possible to identify blood type in a single step. Red blood cells were stained and the plasma phase dyed with fluorescent compounds to enable them to be visualised under the confocal microscope at high magnification. The mechanisms uncovered were in surprising contrast with those found for a similar, paper-based method. Red blood cell aggregates did not flow over each other within the Thread substrate as expected, but suffered from a restriction to their flow which resulted in the chromatographic separation of the RBCs from the liquid phase of the blood. It is hoped that these results will lead to the optimisation of the method to enable more accurate and sensitive detection, increasing the range of blood systems that can be detected.

  • Red blood cell transport mechanisms in Polyester Thread-based blood typing devices
    Analytical and Bioanalytical Chemistry, 2016
    Co-Authors: Azadeh Nilghaz, David Robert Ballerini, Liyun Guan, Lizi Li, Wei Shen
    Abstract:

    A recently developed blood typing diagnostic based on a Polyester Thread substrate has shown great promise for use in medical emergencies and in impoverished regions. The device is easy to use and transport, while also being inexpensive, accurate, and rapid. This study used a fluorescent confocal microscope to delve deeper into how red blood cells were behaving within the Polyester Thread-based diagnostic at the cellular level, and how plasma separation could be made to visibly occur on the Thread, making it possible to identify blood type in a single step. Red blood cells were stained and the plasma phase dyed with fluorescent compounds to enable them to be visualised under the confocal microscope at high magnification. The mechanisms uncovered were in surprising contrast with those found for a similar, paper-based method. Red blood cell aggregates did not flow over each other within the Thread substrate as expected, but suffered from a restriction to their flow which resulted in the chromatographic separation of the RBCs from the liquid phase of the blood. It is hoped that these results will lead to the optimisation of the method to enable more accurate and sensitive detection, increasing the range of blood systems that can be detected. Graphical Abstract Agglutinated FITC stained A+ blood on anti-A antibody treated Thread. Sheet like structures composed of agglutinated RBCs can be seen, wrapped around fibres and occupying the spaces between them

Azadeh Nilghaz - One of the best experts on this subject based on the ideXlab platform.

  • red blood cell transport mechanisms in Polyester Thread based blood typing devices
    Analytical and Bioanalytical Chemistry, 2016
    Co-Authors: Azadeh Nilghaz, David Robert Ballerini, Liyun Guan, Lizi Li, Wei Shen
    Abstract:

    A recently developed blood typing diagnostic based on a Polyester Thread substrate has shown great promise for use in medical emergencies and in impoverished regions. The device is easy to use and transport, while also being inexpensive, accurate, and rapid. This study used a fluorescent confocal microscope to delve deeper into how red blood cells were behaving within the Polyester Thread-based diagnostic at the cellular level, and how plasma separation could be made to visibly occur on the Thread, making it possible to identify blood type in a single step. Red blood cells were stained and the plasma phase dyed with fluorescent compounds to enable them to be visualised under the confocal microscope at high magnification. The mechanisms uncovered were in surprising contrast with those found for a similar, paper-based method. Red blood cell aggregates did not flow over each other within the Thread substrate as expected, but suffered from a restriction to their flow which resulted in the chromatographic separation of the RBCs from the liquid phase of the blood. It is hoped that these results will lead to the optimisation of the method to enable more accurate and sensitive detection, increasing the range of blood systems that can be detected.

  • Red blood cell transport mechanisms in Polyester Thread-based blood typing devices
    Analytical and Bioanalytical Chemistry, 2016
    Co-Authors: Azadeh Nilghaz, David Robert Ballerini, Liyun Guan, Lizi Li, Wei Shen
    Abstract:

    A recently developed blood typing diagnostic based on a Polyester Thread substrate has shown great promise for use in medical emergencies and in impoverished regions. The device is easy to use and transport, while also being inexpensive, accurate, and rapid. This study used a fluorescent confocal microscope to delve deeper into how red blood cells were behaving within the Polyester Thread-based diagnostic at the cellular level, and how plasma separation could be made to visibly occur on the Thread, making it possible to identify blood type in a single step. Red blood cells were stained and the plasma phase dyed with fluorescent compounds to enable them to be visualised under the confocal microscope at high magnification. The mechanisms uncovered were in surprising contrast with those found for a similar, paper-based method. Red blood cell aggregates did not flow over each other within the Thread substrate as expected, but suffered from a restriction to their flow which resulted in the chromatographic separation of the RBCs from the liquid phase of the blood. It is hoped that these results will lead to the optimisation of the method to enable more accurate and sensitive detection, increasing the range of blood systems that can be detected. Graphical Abstract Agglutinated FITC stained A+ blood on anti-A antibody treated Thread. Sheet like structures composed of agglutinated RBCs can be seen, wrapped around fibres and occupying the spaces between them

Lizi Li - One of the best experts on this subject based on the ideXlab platform.

  • red blood cell transport mechanisms in Polyester Thread based blood typing devices
    Analytical and Bioanalytical Chemistry, 2016
    Co-Authors: Azadeh Nilghaz, David Robert Ballerini, Liyun Guan, Lizi Li, Wei Shen
    Abstract:

    A recently developed blood typing diagnostic based on a Polyester Thread substrate has shown great promise for use in medical emergencies and in impoverished regions. The device is easy to use and transport, while also being inexpensive, accurate, and rapid. This study used a fluorescent confocal microscope to delve deeper into how red blood cells were behaving within the Polyester Thread-based diagnostic at the cellular level, and how plasma separation could be made to visibly occur on the Thread, making it possible to identify blood type in a single step. Red blood cells were stained and the plasma phase dyed with fluorescent compounds to enable them to be visualised under the confocal microscope at high magnification. The mechanisms uncovered were in surprising contrast with those found for a similar, paper-based method. Red blood cell aggregates did not flow over each other within the Thread substrate as expected, but suffered from a restriction to their flow which resulted in the chromatographic separation of the RBCs from the liquid phase of the blood. It is hoped that these results will lead to the optimisation of the method to enable more accurate and sensitive detection, increasing the range of blood systems that can be detected.

  • Red blood cell transport mechanisms in Polyester Thread-based blood typing devices
    Analytical and Bioanalytical Chemistry, 2016
    Co-Authors: Azadeh Nilghaz, David Robert Ballerini, Liyun Guan, Lizi Li, Wei Shen
    Abstract:

    A recently developed blood typing diagnostic based on a Polyester Thread substrate has shown great promise for use in medical emergencies and in impoverished regions. The device is easy to use and transport, while also being inexpensive, accurate, and rapid. This study used a fluorescent confocal microscope to delve deeper into how red blood cells were behaving within the Polyester Thread-based diagnostic at the cellular level, and how plasma separation could be made to visibly occur on the Thread, making it possible to identify blood type in a single step. Red blood cells were stained and the plasma phase dyed with fluorescent compounds to enable them to be visualised under the confocal microscope at high magnification. The mechanisms uncovered were in surprising contrast with those found for a similar, paper-based method. Red blood cell aggregates did not flow over each other within the Thread substrate as expected, but suffered from a restriction to their flow which resulted in the chromatographic separation of the RBCs from the liquid phase of the blood. It is hoped that these results will lead to the optimisation of the method to enable more accurate and sensitive detection, increasing the range of blood systems that can be detected. Graphical Abstract Agglutinated FITC stained A+ blood on anti-A antibody treated Thread. Sheet like structures composed of agglutinated RBCs can be seen, wrapped around fibres and occupying the spaces between them

David Robert Ballerini - One of the best experts on this subject based on the ideXlab platform.

  • red blood cell transport mechanisms in Polyester Thread based blood typing devices
    Analytical and Bioanalytical Chemistry, 2016
    Co-Authors: Azadeh Nilghaz, David Robert Ballerini, Liyun Guan, Lizi Li, Wei Shen
    Abstract:

    A recently developed blood typing diagnostic based on a Polyester Thread substrate has shown great promise for use in medical emergencies and in impoverished regions. The device is easy to use and transport, while also being inexpensive, accurate, and rapid. This study used a fluorescent confocal microscope to delve deeper into how red blood cells were behaving within the Polyester Thread-based diagnostic at the cellular level, and how plasma separation could be made to visibly occur on the Thread, making it possible to identify blood type in a single step. Red blood cells were stained and the plasma phase dyed with fluorescent compounds to enable them to be visualised under the confocal microscope at high magnification. The mechanisms uncovered were in surprising contrast with those found for a similar, paper-based method. Red blood cell aggregates did not flow over each other within the Thread substrate as expected, but suffered from a restriction to their flow which resulted in the chromatographic separation of the RBCs from the liquid phase of the blood. It is hoped that these results will lead to the optimisation of the method to enable more accurate and sensitive detection, increasing the range of blood systems that can be detected.

  • Red blood cell transport mechanisms in Polyester Thread-based blood typing devices
    Analytical and Bioanalytical Chemistry, 2016
    Co-Authors: Azadeh Nilghaz, David Robert Ballerini, Liyun Guan, Lizi Li, Wei Shen
    Abstract:

    A recently developed blood typing diagnostic based on a Polyester Thread substrate has shown great promise for use in medical emergencies and in impoverished regions. The device is easy to use and transport, while also being inexpensive, accurate, and rapid. This study used a fluorescent confocal microscope to delve deeper into how red blood cells were behaving within the Polyester Thread-based diagnostic at the cellular level, and how plasma separation could be made to visibly occur on the Thread, making it possible to identify blood type in a single step. Red blood cells were stained and the plasma phase dyed with fluorescent compounds to enable them to be visualised under the confocal microscope at high magnification. The mechanisms uncovered were in surprising contrast with those found for a similar, paper-based method. Red blood cell aggregates did not flow over each other within the Thread substrate as expected, but suffered from a restriction to their flow which resulted in the chromatographic separation of the RBCs from the liquid phase of the blood. It is hoped that these results will lead to the optimisation of the method to enable more accurate and sensitive detection, increasing the range of blood systems that can be detected. Graphical Abstract Agglutinated FITC stained A+ blood on anti-A antibody treated Thread. Sheet like structures composed of agglutinated RBCs can be seen, wrapped around fibres and occupying the spaces between them

Liyun Guan - One of the best experts on this subject based on the ideXlab platform.

  • red blood cell transport mechanisms in Polyester Thread based blood typing devices
    Analytical and Bioanalytical Chemistry, 2016
    Co-Authors: Azadeh Nilghaz, David Robert Ballerini, Liyun Guan, Lizi Li, Wei Shen
    Abstract:

    A recently developed blood typing diagnostic based on a Polyester Thread substrate has shown great promise for use in medical emergencies and in impoverished regions. The device is easy to use and transport, while also being inexpensive, accurate, and rapid. This study used a fluorescent confocal microscope to delve deeper into how red blood cells were behaving within the Polyester Thread-based diagnostic at the cellular level, and how plasma separation could be made to visibly occur on the Thread, making it possible to identify blood type in a single step. Red blood cells were stained and the plasma phase dyed with fluorescent compounds to enable them to be visualised under the confocal microscope at high magnification. The mechanisms uncovered were in surprising contrast with those found for a similar, paper-based method. Red blood cell aggregates did not flow over each other within the Thread substrate as expected, but suffered from a restriction to their flow which resulted in the chromatographic separation of the RBCs from the liquid phase of the blood. It is hoped that these results will lead to the optimisation of the method to enable more accurate and sensitive detection, increasing the range of blood systems that can be detected.

  • Red blood cell transport mechanisms in Polyester Thread-based blood typing devices
    Analytical and Bioanalytical Chemistry, 2016
    Co-Authors: Azadeh Nilghaz, David Robert Ballerini, Liyun Guan, Lizi Li, Wei Shen
    Abstract:

    A recently developed blood typing diagnostic based on a Polyester Thread substrate has shown great promise for use in medical emergencies and in impoverished regions. The device is easy to use and transport, while also being inexpensive, accurate, and rapid. This study used a fluorescent confocal microscope to delve deeper into how red blood cells were behaving within the Polyester Thread-based diagnostic at the cellular level, and how plasma separation could be made to visibly occur on the Thread, making it possible to identify blood type in a single step. Red blood cells were stained and the plasma phase dyed with fluorescent compounds to enable them to be visualised under the confocal microscope at high magnification. The mechanisms uncovered were in surprising contrast with those found for a similar, paper-based method. Red blood cell aggregates did not flow over each other within the Thread substrate as expected, but suffered from a restriction to their flow which resulted in the chromatographic separation of the RBCs from the liquid phase of the blood. It is hoped that these results will lead to the optimisation of the method to enable more accurate and sensitive detection, increasing the range of blood systems that can be detected. Graphical Abstract Agglutinated FITC stained A+ blood on anti-A antibody treated Thread. Sheet like structures composed of agglutinated RBCs can be seen, wrapped around fibres and occupying the spaces between them