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Marcia J Kieliszewski - One of the best experts on this subject based on the ideXlab platform.

  • structure of a hydroxyproline hyp arabinogalactan Polysaccharide from repetitive ala hyp expressed in transgenic nicotiana tabacum
    Journal of Biological Chemistry, 2004
    Co-Authors: Derek T A Lamport, Marcia J Kieliszewski
    Abstract:

    Abstract A synthetic gene encoding the fusion protein (Ala-Hyp)51-enhanced green fluorescent protein expressed in Nicotiana tabacum cells produced a fusion glycoprotein with all proline residues hydroxylated and substituted with an arabinogalactan Polysaccharide. Alkaline hydrolysis of the fusion glycoprotein yielded a population of hydroxyproline (Hyp)-arabinogalactan Polysaccharides ranging in size from 13 to 26 saccharide residues/Hyp, with a median size of 15-17 residues. We isolated a 15-residue Hyp-arabinogalactan for structure determination by sugar analyses and one- and two-dimensional nuclear magnetic resonance techniques that provided the assignment of proton and carbon signals of a small Polysaccharide O-linked to the hydroxyl group of Hyp. The Polysaccharide consisted of a 1,3-linked β-d-Galp backbone with a single 1,6-linked β-d-Galp “kink.” The backbone had two side chains of Galp substituted at position 3 with an arabinose di- or trisaccharide and at position 6 with glucuronic acid or rhamnosyl glucuronic acid. Energy-minimized space-filling molecular models showed hydrogen bonding within Polysaccharides attached to repetitive Ala-Hyp and also between Polysaccharides and the peptide backbone. Polysaccharides distorted the peptide Ramachandran angles consistent with the circular dichroic spectra of isolated (Ala-Hyp)51 and its reversion to a polyproline II-like helix after deglycosylation. This first complete structure of a Hyp-arabinogalactan Polysaccharide shows that computer-based molecular modeling of Hyp-rich glycoproteins is now feasible and supports the suggestion that small repetitive subunits comprise larger arabinogalactan Polysaccharides.

  • structure of a hydroxyproline hyp arabinogalactan Polysaccharide from repetitive ala hyp expressed in transgenic nicotiana tabacum
    Journal of Biological Chemistry, 2004
    Co-Authors: Li Tan, Derek T A Lamport, Feng Qiu, Marcia J Kieliszewski
    Abstract:

    A synthetic gene encoding the fusion protein (Ala-Hyp)(51)-enhanced green fluorescent protein expressed in Nicotiana tabacum cells produced a fusion glycoprotein with all proline residues hydroxylated and substituted with an arabinogalactan Polysaccharide. Alkaline hydrolysis of the fusion glycoprotein yielded a population of hydroxyproline (Hyp)-arabinogalactan Polysaccharides ranging in size from 13 to 26 saccharide residues/Hyp, with a median size of 15-17 residues. We isolated a 15-residue Hyp-arabinogalactan for structure determination by sugar analyses and one- and two-dimensional nuclear magnetic resonance techniques that provided the assignment of proton and carbon signals of a small Polysaccharide O-linked to the hydroxyl group of Hyp. The Polysaccharide consisted of a 1,3-linked beta-D-Galp backbone with a single 1,6-linked beta-D-Galp "kink." The backbone had two side chains of Galp substituted at position 3 with an arabinose di- or trisaccharide and at position 6 with glucuronic acid or rhamnosyl glucuronic acid. Energy-minimized space-filling molecular models showed hydrogen bonding within Polysaccharides attached to repetitive Ala-Hyp and also between Polysaccharides and the peptide backbone. Polysaccharides distorted the peptide Ramachandran angles consistent with the circular dichroic spectra of isolated (Ala-Hyp)(51) and its reversion to a polyproline II-like helix after deglycosylation. This first complete structure of a Hyp-arabinogalactan Polysaccharide shows that computer-based molecular modeling of Hyp-rich glycoproteins is now feasible and supports the suggestion that small repetitive subunits comprise larger arabinogalactan Polysaccharides.

Yueming Jiang - One of the best experts on this subject based on the ideXlab platform.

  • the structure changes of water soluble Polysaccharides in papaya during ripening
    International Journal of Biological Macromolecules, 2018
    Co-Authors: Afiya John, Yueming Jiang, Jiali Yang, Juan Liu, Bao Yang
    Abstract:

    Papaya is a fruit mainly grown in tropical and subtropical regions. The structural changes of Polysaccharides are highly involved in the organoleptic property change of papaya. However, it remains unclear how the structure characteristics of water-soluble Polysaccharides are changed during postharvest ripening. It is interesting to work on this topic. In this work, the dominant water-soluble Polysaccharide in papaya was purified. It was identified to be α-(1→4)-D-galacturonan by NMR. It remained stable during postharvest ripening. β-(1→4)-D-galactan was only detected in fully green papaya. Once the postharvest ripening initiated, this Polysaccharide was rapidly degraded. There were no significant differences in the structure changes of water-soluble Polysaccharides for the naturally ripening papaya and ethylene-treated papaya. These results extended the understanding about the relationship between Polysaccharide structure and papaya ripening during postharvest storage.

  • identification of Polysaccharides from pericarp tissues of litchi litchi chinensis sonn fruit in relation to their antioxidant activities
    Carbohydrate Research, 2006
    Co-Authors: Bao Yang, Jinshui Wang, Mourning Zhao, Wei Wang, Yueming Jiang
    Abstract:

    Abstract A large number of Polysaccharides are present in the pericarp tissues of harvested litchi fruits. A DEAE Sepharose fast-flow anion-exchange column and a Sephadex G-50 gel-permeation column were used to isolate and purify the major Polysaccharides from litchi fruit pericarp tissues. Antioxidant activities of these major Polysaccharide components were also evaluated. An aqueous extract of the Polysaccharides from litchi fruit pericarp tissues was chromatographed on a DEAE anion-exchange column to yield two fractions. The largest amount of the Polysaccharide fraction was subjected to further purification by gel filtration on Sephadex G-50. The purified product was a neutral Polysaccharide, with a molecular weight of 14 kDa, comprised mainly of 65.6% mannose, 33.0% galactose and 1.4% arabinose. Analysis by Smith degradation indicated that there were 8.7% of (1→2)-glycosidic linkages, 83.3% of (1→3)-glycosidic linkages and 8.0% of (1→6)-glycosidic linkages in the Polysaccharide. Furthermore, different Polysaccharide fractions extracted and purified from litchi fruit pericarp tissues exhibited strong antioxidant activities. Among these fractions, the purified Polysaccharide had the highest antioxidant activity and should be explored as a novel potential antioxidant.

  • identification of Polysaccharides from pericarp tissues of litchi litchi chinensis sonn fruit in relation to their antioxidant activities
    Carbohydrate Research, 2006
    Co-Authors: Bao Yang, Jinshui Wang, Mourning Zhao, Wei Wang, Yang Liu, Yueming Jiang
    Abstract:

    Abstract A large number of Polysaccharides are present in the pericarp tissues of harvested litchi fruits. A DEAE Sepharose fast-flow anion-exchange column and a Sephadex G-50 gel-permeation column were used to isolate and purify the major Polysaccharides from litchi fruit pericarp tissues. Antioxidant activities of these major Polysaccharide components were also evaluated. An aqueous extract of the Polysaccharides from litchi fruit pericarp tissues was chromatographed on a DEAE anion-exchange column to yield two fractions. The largest amount of the Polysaccharide fraction was subjected to further purification by gel filtration on Sephadex G-50. The purified product was a neutral Polysaccharide, with a molecular weight of 14 kDa, comprised mainly of 65.6% mannose, 33.0% galactose and 1.4% arabinose. Analysis by Smith degradation indicated that there were 8.7% of (1→2)-glycosidic linkages, 83.3% of (1→3)-glycosidic linkages and 8.0% of (1→6)-glycosidic linkages in the Polysaccharide. Furthermore, different Polysaccharide fractions extracted and purified from litchi fruit pericarp tissues exhibited strong antioxidant activities. Among these fractions, the purified Polysaccharide had the highest antioxidant activity and should be explored as a novel potential antioxidant.

Bao Yang - One of the best experts on this subject based on the ideXlab platform.

  • the structure changes of water soluble Polysaccharides in papaya during ripening
    International Journal of Biological Macromolecules, 2018
    Co-Authors: Afiya John, Yueming Jiang, Jiali Yang, Juan Liu, Bao Yang
    Abstract:

    Papaya is a fruit mainly grown in tropical and subtropical regions. The structural changes of Polysaccharides are highly involved in the organoleptic property change of papaya. However, it remains unclear how the structure characteristics of water-soluble Polysaccharides are changed during postharvest ripening. It is interesting to work on this topic. In this work, the dominant water-soluble Polysaccharide in papaya was purified. It was identified to be α-(1→4)-D-galacturonan by NMR. It remained stable during postharvest ripening. β-(1→4)-D-galactan was only detected in fully green papaya. Once the postharvest ripening initiated, this Polysaccharide was rapidly degraded. There were no significant differences in the structure changes of water-soluble Polysaccharides for the naturally ripening papaya and ethylene-treated papaya. These results extended the understanding about the relationship between Polysaccharide structure and papaya ripening during postharvest storage.

  • identification of Polysaccharides from pericarp tissues of litchi litchi chinensis sonn fruit in relation to their antioxidant activities
    Carbohydrate Research, 2006
    Co-Authors: Bao Yang, Jinshui Wang, Mourning Zhao, Wei Wang, Yueming Jiang
    Abstract:

    Abstract A large number of Polysaccharides are present in the pericarp tissues of harvested litchi fruits. A DEAE Sepharose fast-flow anion-exchange column and a Sephadex G-50 gel-permeation column were used to isolate and purify the major Polysaccharides from litchi fruit pericarp tissues. Antioxidant activities of these major Polysaccharide components were also evaluated. An aqueous extract of the Polysaccharides from litchi fruit pericarp tissues was chromatographed on a DEAE anion-exchange column to yield two fractions. The largest amount of the Polysaccharide fraction was subjected to further purification by gel filtration on Sephadex G-50. The purified product was a neutral Polysaccharide, with a molecular weight of 14 kDa, comprised mainly of 65.6% mannose, 33.0% galactose and 1.4% arabinose. Analysis by Smith degradation indicated that there were 8.7% of (1→2)-glycosidic linkages, 83.3% of (1→3)-glycosidic linkages and 8.0% of (1→6)-glycosidic linkages in the Polysaccharide. Furthermore, different Polysaccharide fractions extracted and purified from litchi fruit pericarp tissues exhibited strong antioxidant activities. Among these fractions, the purified Polysaccharide had the highest antioxidant activity and should be explored as a novel potential antioxidant.

  • identification of Polysaccharides from pericarp tissues of litchi litchi chinensis sonn fruit in relation to their antioxidant activities
    Carbohydrate Research, 2006
    Co-Authors: Bao Yang, Jinshui Wang, Mourning Zhao, Wei Wang, Yang Liu, Yueming Jiang
    Abstract:

    Abstract A large number of Polysaccharides are present in the pericarp tissues of harvested litchi fruits. A DEAE Sepharose fast-flow anion-exchange column and a Sephadex G-50 gel-permeation column were used to isolate and purify the major Polysaccharides from litchi fruit pericarp tissues. Antioxidant activities of these major Polysaccharide components were also evaluated. An aqueous extract of the Polysaccharides from litchi fruit pericarp tissues was chromatographed on a DEAE anion-exchange column to yield two fractions. The largest amount of the Polysaccharide fraction was subjected to further purification by gel filtration on Sephadex G-50. The purified product was a neutral Polysaccharide, with a molecular weight of 14 kDa, comprised mainly of 65.6% mannose, 33.0% galactose and 1.4% arabinose. Analysis by Smith degradation indicated that there were 8.7% of (1→2)-glycosidic linkages, 83.3% of (1→3)-glycosidic linkages and 8.0% of (1→6)-glycosidic linkages in the Polysaccharide. Furthermore, different Polysaccharide fractions extracted and purified from litchi fruit pericarp tissues exhibited strong antioxidant activities. Among these fractions, the purified Polysaccharide had the highest antioxidant activity and should be explored as a novel potential antioxidant.

Derek T A Lamport - One of the best experts on this subject based on the ideXlab platform.

  • structure of a hydroxyproline hyp arabinogalactan Polysaccharide from repetitive ala hyp expressed in transgenic nicotiana tabacum
    Journal of Biological Chemistry, 2004
    Co-Authors: Derek T A Lamport, Marcia J Kieliszewski
    Abstract:

    Abstract A synthetic gene encoding the fusion protein (Ala-Hyp)51-enhanced green fluorescent protein expressed in Nicotiana tabacum cells produced a fusion glycoprotein with all proline residues hydroxylated and substituted with an arabinogalactan Polysaccharide. Alkaline hydrolysis of the fusion glycoprotein yielded a population of hydroxyproline (Hyp)-arabinogalactan Polysaccharides ranging in size from 13 to 26 saccharide residues/Hyp, with a median size of 15-17 residues. We isolated a 15-residue Hyp-arabinogalactan for structure determination by sugar analyses and one- and two-dimensional nuclear magnetic resonance techniques that provided the assignment of proton and carbon signals of a small Polysaccharide O-linked to the hydroxyl group of Hyp. The Polysaccharide consisted of a 1,3-linked β-d-Galp backbone with a single 1,6-linked β-d-Galp “kink.” The backbone had two side chains of Galp substituted at position 3 with an arabinose di- or trisaccharide and at position 6 with glucuronic acid or rhamnosyl glucuronic acid. Energy-minimized space-filling molecular models showed hydrogen bonding within Polysaccharides attached to repetitive Ala-Hyp and also between Polysaccharides and the peptide backbone. Polysaccharides distorted the peptide Ramachandran angles consistent with the circular dichroic spectra of isolated (Ala-Hyp)51 and its reversion to a polyproline II-like helix after deglycosylation. This first complete structure of a Hyp-arabinogalactan Polysaccharide shows that computer-based molecular modeling of Hyp-rich glycoproteins is now feasible and supports the suggestion that small repetitive subunits comprise larger arabinogalactan Polysaccharides.

  • structure of a hydroxyproline hyp arabinogalactan Polysaccharide from repetitive ala hyp expressed in transgenic nicotiana tabacum
    Journal of Biological Chemistry, 2004
    Co-Authors: Li Tan, Derek T A Lamport, Feng Qiu, Marcia J Kieliszewski
    Abstract:

    A synthetic gene encoding the fusion protein (Ala-Hyp)(51)-enhanced green fluorescent protein expressed in Nicotiana tabacum cells produced a fusion glycoprotein with all proline residues hydroxylated and substituted with an arabinogalactan Polysaccharide. Alkaline hydrolysis of the fusion glycoprotein yielded a population of hydroxyproline (Hyp)-arabinogalactan Polysaccharides ranging in size from 13 to 26 saccharide residues/Hyp, with a median size of 15-17 residues. We isolated a 15-residue Hyp-arabinogalactan for structure determination by sugar analyses and one- and two-dimensional nuclear magnetic resonance techniques that provided the assignment of proton and carbon signals of a small Polysaccharide O-linked to the hydroxyl group of Hyp. The Polysaccharide consisted of a 1,3-linked beta-D-Galp backbone with a single 1,6-linked beta-D-Galp "kink." The backbone had two side chains of Galp substituted at position 3 with an arabinose di- or trisaccharide and at position 6 with glucuronic acid or rhamnosyl glucuronic acid. Energy-minimized space-filling molecular models showed hydrogen bonding within Polysaccharides attached to repetitive Ala-Hyp and also between Polysaccharides and the peptide backbone. Polysaccharides distorted the peptide Ramachandran angles consistent with the circular dichroic spectra of isolated (Ala-Hyp)(51) and its reversion to a polyproline II-like helix after deglycosylation. This first complete structure of a Hyp-arabinogalactan Polysaccharide shows that computer-based molecular modeling of Hyp-rich glycoproteins is now feasible and supports the suggestion that small repetitive subunits comprise larger arabinogalactan Polysaccharides.

Hai-ning Zhuang - One of the best experts on this subject based on the ideXlab platform.

  • ultrasound assisted extraction of Polysaccharides from volvariella volvacea process optimization and structural characterization
    Molecules, 2018
    Co-Authors: Fengjie Cui, Li-sun Qian, Wen-jing Sun, Jin-song Zhang, Yan Yang, Hai-ning Zhuang
    Abstract:

    The aims of the present study were to optimize the operational parameters to maximize the yield of ultrasound-assisted Polysaccharide extraction from Volvariella volvacea (straw mushroom) fruiting bodies by using for the first time one-factor-at-a-time and three-level Box-Behnken factorial designs. A maximum Polysaccharide yield of 8.28 ± 0.23% was obtained under the optimized conditions of ultrasound power of 175 W, extraction temperature of 57 °C, extraction time of 33 min, and the ratio of liquid to raw material of 25:1, respectively. Compared to the hot-water extraction, the ultrasound-assistance favored the extraction of Polysaccharides from V. volvacea for its higher Polysaccharide yield and efficiency. Further preliminary Polysaccharide structural characterization indicated that ultrasound treatment affected the monosaccharide compositions and ratios, and molecular weight range of Polysaccharides extracted from V. volvacea.

  • Ultrasound-Assisted Extraction of Polysaccharides from Volvariella volvacea: Process Optimization and Structural Characterization
    MDPI AG, 2018
    Co-Authors: Fengjie Cui, Li-sun Qian, Wen-jing Sun, Jin-song Zhang, Yan Yang, Hai-ning Zhuang
    Abstract:

    The aims of the present study were to optimize the operational parameters to maximize the yield of ultrasound-assisted Polysaccharide extraction from Volvariella volvacea (straw mushroom) fruiting bodies by using for the first time one-factor-at-a-time and three-level Box-Behnken factorial designs. A maximum Polysaccharide yield of 8.28 ± 0.23% was obtained under the optimized conditions of ultrasound power of 175 W, extraction temperature of 57 °C, extraction time of 33 min, and the ratio of liquid to raw material of 25:1, respectively. Compared to the hot-water extraction, the ultrasound-assistance favored the extraction of Polysaccharides from V. volvacea for its higher Polysaccharide yield and efficiency. Further preliminary Polysaccharide structural characterization indicated that ultrasound treatment affected the monosaccharide compositions and ratios, and molecular weight range of Polysaccharides extracted from V. volvacea