The Experts below are selected from a list of 21 Experts worldwide ranked by ideXlab platform
V. L. Rapoport - One of the best experts on this subject based on the ideXlab platform.
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A study of the spectral and luminescence manifestations of the aggregation of thymine chromophores in Polythymidylic Acid aqueous solutions at room temperature in the context of photochemical information recording using thymine
Biophysics, 2008Co-Authors: V. M. Malkin, V. L. RapoportAbstract:The luminescence and excitation spectra of Polythymidylic Acid aqueous solutions at room temperature were studied. In addition to the previously described band at 338 nm, two new bands at 320 and 350 nm were recorded at various excitation wavelengths. An examination of the excitation spectra that had not been studied previously, as well as their comparison with the differential absorption spectra previously recorded during photodimerization, allowed us to interpret the band at 320 nm as the band of noninteracting chromophores; the band at 338 nm as the band of the most photochemically active, densely packed stacking dimmers (exciton splitting of absorption band of ∼4000 cm^−1); and the band at 350 nm as the band of photochemically inactive large stacking aggregates ( n ≥ 10, exciton splitting of ∼8000 cm^−1). The changes in the optical density of the Polythymidylic Acid aqueous solution at γ = 270 nm after successive irradiation of the solution with light at 279 + 302 and 248 nm were studied. The reasons for their incomplete reversibility are discussed.
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study of spectral and luminescent manifestations of the aggregation of thymine chromophores in aqueous solutions of Polythymidylic Acid at room temperature in connection with the task of photochemical record of information on thymine
Biofizika, 2008Co-Authors: V. M. Malkin, V. L. RapoportAbstract:Luminescence and excitation luminescence spectra of water solutions of Polythymidylic Acid at room temperature were studied. Three luminescence bands at different excitation wavelengths were observed: at 338 nm, which was known earlier, and two new bands, at 320 and 350 nm. The study of excitation luminescence spectra that have not been studied earlier led us to interpret the band at 320 nm as a band of chromophores that do not interact, the band at 338 nm as a band of photochemically most active densely packed stacking dimers (absorption band exciton splitting approximately 4000 cm(-1)), and the band at 350 nm as a band of photochemically inactive big stacking aggregates (n > or = 10, exciton splitting approximately 8000 cm(-1)). Changes in optical density at 270 nm of poly-T water solutions after consecutive irradiations with UV light at 297+302 and 248 nm were studied. The causes of incomplete reversibility are discussed.
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photochemical revelation of the stacking aggregation of thymine chromophores in water solutions of Polythymidylic Acid
Biofizika, 2005Co-Authors: V. M. Malkin, V. L. RapoportAbstract:The structure heterogeneity of water solutions of polyribothymidylic Acid at T(room) was studied from changes caused in their absorption spectra by the photodimerization reaction. Three fractions of thymine chromophores were revealed from the differential absorption spectra: (a) the main fraction consisting of weakly interacting (isolated chromophores) chromophores with the absorption spectrum maximum at approximately 270 nm; (b) pair chromophores of the first type with the absorption spectrum maxima at 260 and 290 nm (exciton splitting 4000 cm(-1)); and (c) pair chromophores of the second type with the absorption spectrum maxima at 250 and 280 nm (exciton splitting 4300 cm(-1)). The revealed aggregates have a relatively high photochemical activity in the photodimerization reaction in comparison with the isolated chromophores. They contribute little to the total absorption spectrum of solutions but make a great contribution to its changes at the initial stages of the UV irradiation of solutions.
V. M. Malkin - One of the best experts on this subject based on the ideXlab platform.
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A study of the spectral and luminescence manifestations of the aggregation of thymine chromophores in Polythymidylic Acid aqueous solutions at room temperature in the context of photochemical information recording using thymine
Biophysics, 2008Co-Authors: V. M. Malkin, V. L. RapoportAbstract:The luminescence and excitation spectra of Polythymidylic Acid aqueous solutions at room temperature were studied. In addition to the previously described band at 338 nm, two new bands at 320 and 350 nm were recorded at various excitation wavelengths. An examination of the excitation spectra that had not been studied previously, as well as their comparison with the differential absorption spectra previously recorded during photodimerization, allowed us to interpret the band at 320 nm as the band of noninteracting chromophores; the band at 338 nm as the band of the most photochemically active, densely packed stacking dimmers (exciton splitting of absorption band of ∼4000 cm^−1); and the band at 350 nm as the band of photochemically inactive large stacking aggregates ( n ≥ 10, exciton splitting of ∼8000 cm^−1). The changes in the optical density of the Polythymidylic Acid aqueous solution at γ = 270 nm after successive irradiation of the solution with light at 279 + 302 and 248 nm were studied. The reasons for their incomplete reversibility are discussed.
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study of spectral and luminescent manifestations of the aggregation of thymine chromophores in aqueous solutions of Polythymidylic Acid at room temperature in connection with the task of photochemical record of information on thymine
Biofizika, 2008Co-Authors: V. M. Malkin, V. L. RapoportAbstract:Luminescence and excitation luminescence spectra of water solutions of Polythymidylic Acid at room temperature were studied. Three luminescence bands at different excitation wavelengths were observed: at 338 nm, which was known earlier, and two new bands, at 320 and 350 nm. The study of excitation luminescence spectra that have not been studied earlier led us to interpret the band at 320 nm as a band of chromophores that do not interact, the band at 338 nm as a band of photochemically most active densely packed stacking dimers (absorption band exciton splitting approximately 4000 cm(-1)), and the band at 350 nm as a band of photochemically inactive big stacking aggregates (n > or = 10, exciton splitting approximately 8000 cm(-1)). Changes in optical density at 270 nm of poly-T water solutions after consecutive irradiations with UV light at 297+302 and 248 nm were studied. The causes of incomplete reversibility are discussed.
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photochemical revelation of the stacking aggregation of thymine chromophores in water solutions of Polythymidylic Acid
Biofizika, 2005Co-Authors: V. M. Malkin, V. L. RapoportAbstract:The structure heterogeneity of water solutions of polyribothymidylic Acid at T(room) was studied from changes caused in their absorption spectra by the photodimerization reaction. Three fractions of thymine chromophores were revealed from the differential absorption spectra: (a) the main fraction consisting of weakly interacting (isolated chromophores) chromophores with the absorption spectrum maximum at approximately 270 nm; (b) pair chromophores of the first type with the absorption spectrum maxima at 260 and 290 nm (exciton splitting 4000 cm(-1)); and (c) pair chromophores of the second type with the absorption spectrum maxima at 250 and 280 nm (exciton splitting 4300 cm(-1)). The revealed aggregates have a relatively high photochemical activity in the photodimerization reaction in comparison with the isolated chromophores. They contribute little to the total absorption spectrum of solutions but make a great contribution to its changes at the initial stages of the UV irradiation of solutions.
Junichi Yano - One of the best experts on this subject based on the ideXlab platform.
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formation of triplexes of diastereoisomers of 2 o methyladenylyl 3 5 2 o methyladenosine ethylphosphotriesters and 2 o methyladenylyl 3 5 2 o methyladenosine methyl phosphonates with polyuridylic Acid and Polythymidylic Acid a steric effect
Journal of The Chinese Chemical Society, 1993Co-Authors: Lousing Kan, William Koo, Junichi YanoAbstract:The triplex formation of diastereoisomers of 2′-O-methyladenylyl-3′,5′-2′-O-methyladenosine ethylphosphotriesters and 2′-O-methyladenylyl-3′,5′-2′-O-methyladenosinc methyl phosphonates with polyuridylic Acid and Polythymidylic Acid was monitored by UV spectral mediods. Possible steric effects on the stability of the triplex are discussed with the aid of simulated chemical structures.
Lousing Kan - One of the best experts on this subject based on the ideXlab platform.
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formation of triplexes of diastereoisomers of 2 o methyladenylyl 3 5 2 o methyladenosine ethylphosphotriesters and 2 o methyladenylyl 3 5 2 o methyladenosine methyl phosphonates with polyuridylic Acid and Polythymidylic Acid a steric effect
Journal of The Chinese Chemical Society, 1993Co-Authors: Lousing Kan, William Koo, Junichi YanoAbstract:The triplex formation of diastereoisomers of 2′-O-methyladenylyl-3′,5′-2′-O-methyladenosine ethylphosphotriesters and 2′-O-methyladenylyl-3′,5′-2′-O-methyladenosinc methyl phosphonates with polyuridylic Acid and Polythymidylic Acid was monitored by UV spectral mediods. Possible steric effects on the stability of the triplex are discussed with the aid of simulated chemical structures.
Qiwei Qin - One of the best experts on this subject based on the ideXlab platform.
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isolation and characterization of tumor necrosis factor receptor associated factor 6 traf6 from grouper epinephelus tauvina
Fish & Shellfish Immunology, 2014Co-Authors: Jingguang Wei, Minglan Guo, Pin Gao, Yang Yan, Qiwei QinAbstract:Tumor necrosis factor receptor-associated factor 6 (TRAF6) is one of the key adapter molecules in Toll-like receptor signal transduction that triggers downstream cascades involved in innate immunity. In the present study, a TRAF6 (named as Et-TRAF6) was identified from the marine fish grouper, Epinephelus tauvina by RACE PCR. The full-length cDNA of Et-TRAF6 comprised 1949 bp with a 1713 bp open reading frame (ORF) that encodes a putative protein of 570 amino Acids. Similar to most TRAF6s, Et-TRAF6 includes one N-terminal RING domain (78aa-116aa), two zinc fingers of TRAF-type (159aa-210aa and 212aa-269aa), one coiled-coil region (370aa-394aa), and one conserved C-terminal meprin and TRAF homology (MATH) domain (401aa-526aa). Quantitative real-time PCR analysis revealed that Et-TRAF6 mRNA is expressed in all tested tissues, with the predominant expression in the stomach and intestine. The expression of Et-TRAF6 was up-regulated in the liver after challenge with Lipoteichoic Acid (LTA), Peptidoglycan (PGN), Zymosan, polyinosine polycytidylic Acid [Poly(I:C)] and Polydeoxyadenylic Acid -Polythymidylic Acid sodium salt [Poly(dA:dT)]. The expression of Et-TRAF6 was also up-regulated in the liver after infection with Vibrio alginolyticus, Singapore grouper iridovirus (SGIV) and grouper nervous necrosis virus (GNNV). Recombinant Et-TRAF6 (rEt-TRAF6) was expressed in Escherichia BL21 (DE3) and purified for mouse anti-Et-TRAF6 serum preparation. Intracellular localization revealed that Et-TRAF6 is distributed in both cytoplasm and nucleus, and predominantly in the cytoplasm. These results together indicated that Et-TRAF6 might be involved in immune responses toward bacterial and virus challenges. (C) 2014 Elsevier Ltd. All rights reserved.