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Anirban Basu - One of the best experts on this subject based on the ideXlab platform.
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interaction of proflavine with the rna polynucleotide polyriboadenylic Acid polyribouridylic Acid photophysical and calorimetric studies
Journal of Biomolecular Structure & Dynamics, 2019Co-Authors: Anirban Basu, Gopinatha Suresh KumarAbstract:The binding of proflavine, an acriflavine derivative, with the RNA polynucletodide polyadenylic Acid–Polyuridylic Acid is investigated here to understand the structural and thermodynamic basis of t...
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Interaction of proflavine with the RNA polynucleotide polyriboadenylic Acid–polyribouridylic Acid: photophysical and calorimetric studies
2019Co-Authors: Anirban Basu, Gopinatha Suresh KumarAbstract:The binding of proflavine, an acriflavine derivative, with the RNA polynucletodide polyadenylic Acid–Polyuridylic Acid is investigated here to understand the structural and thermodynamic basis of the binding process. Such binding data are crucial for designing viable theraperutic agents. Spectroscopic studies clearly suggest a strong binding interaction between proflavine and polyadenylic Acid–Polyuridylic Acid leading to efficient energy transfer between the poly AU base pairs and proflavine. The stoichiometry of proflavine polyadenylic Acid–Polyuridylic Acid binding was independently estimated by continuous variation analysis of Job. An intercalative binding model is envisaged for the binding from hydrodynamic studies. Circular dichroism experiments revealed that the binding induced conformational changes in the RNA, and also led to induction of optical activity in the bound dye molecules. The binding affinity of the complex was deduced to be (6.57 ± 0.75) 105 M−1 at (298.15 ± 0.10) K from isothermal titration calorimetry experiment. Positive entropy and negative enthalpy changes characterized the complexation. The binding was observed to be weaker both at higher temperatures and increased [Na+]. The affinity of binding decreased with increasing [Na+]. When the Gibbs energy was parsed between polyelectrolytic and nonpolyelectropytic components, it surprisingly revealed a higher role for the non-polyelectrolytic forces. These results present new data for developing RNA targeted ligands. Communicated by Ramaswamy H. Sarma
Gopinatha Suresh Kumar - One of the best experts on this subject based on the ideXlab platform.
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interaction of proflavine with the rna polynucleotide polyriboadenylic Acid polyribouridylic Acid photophysical and calorimetric studies
Journal of Biomolecular Structure & Dynamics, 2019Co-Authors: Anirban Basu, Gopinatha Suresh KumarAbstract:The binding of proflavine, an acriflavine derivative, with the RNA polynucletodide polyadenylic Acid–Polyuridylic Acid is investigated here to understand the structural and thermodynamic basis of t...
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Interaction of proflavine with the RNA polynucleotide polyriboadenylic Acid–polyribouridylic Acid: photophysical and calorimetric studies
2019Co-Authors: Anirban Basu, Gopinatha Suresh KumarAbstract:The binding of proflavine, an acriflavine derivative, with the RNA polynucletodide polyadenylic Acid–Polyuridylic Acid is investigated here to understand the structural and thermodynamic basis of the binding process. Such binding data are crucial for designing viable theraperutic agents. Spectroscopic studies clearly suggest a strong binding interaction between proflavine and polyadenylic Acid–Polyuridylic Acid leading to efficient energy transfer between the poly AU base pairs and proflavine. The stoichiometry of proflavine polyadenylic Acid–Polyuridylic Acid binding was independently estimated by continuous variation analysis of Job. An intercalative binding model is envisaged for the binding from hydrodynamic studies. Circular dichroism experiments revealed that the binding induced conformational changes in the RNA, and also led to induction of optical activity in the bound dye molecules. The binding affinity of the complex was deduced to be (6.57 ± 0.75) 105 M−1 at (298.15 ± 0.10) K from isothermal titration calorimetry experiment. Positive entropy and negative enthalpy changes characterized the complexation. The binding was observed to be weaker both at higher temperatures and increased [Na+]. The affinity of binding decreased with increasing [Na+]. When the Gibbs energy was parsed between polyelectrolytic and nonpolyelectropytic components, it surprisingly revealed a higher role for the non-polyelectrolytic forces. These results present new data for developing RNA targeted ligands. Communicated by Ramaswamy H. Sarma
James Edward Eyles - One of the best experts on this subject based on the ideXlab platform.
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immunological responses after immunisation of mice with microparticles containing antigen and single stranded rna Polyuridylic Acid
Vaccine, 2006Co-Authors: Angie Westwood, Stephen J Elvin, Gareth D Healey, Diane E Williamson, James Edward EylesAbstract:Certain toll-like receptor (TLR) agonists, e.g. CpG DNA, can be used as potent vaccine 'adjuvants'. It is known that some sequences of single stranded (ss) RNA stimulate proinflammatory and antiviral responses following interaction with TLR 7 and 8. We have encapsulated ovalbumin (OVA) in the presence and absence of Polyuridylic Acid (poly-U) inside polylactide microparticles. In comparison to microparticles containing only OVA, bulk cultures of bone marrow-derived plasmacytoid and myeloid dendritic cells produced more (P<0.05) IL-12 and interferon (IFN)-alpha when stimulated with microparticles containing OVA and poly-U. Subcutaneous injection of comicroencapsulated OVA and poly-U resulted in statistically elevated levels of serum anti-OVA IgG1 (P<0.05 versus naive mice). Conversely, anti-OVA IgG1 levels in C57 BL6 mice immunised with OVA loaded microparticles (without RNA) were statistically indifferent to naive animals. Furthermore, injection of coencapsulated OVA and poly-U resulted in (P<0.05) greater numbers of OVA specific IFN-gamma secreting T-cells as compared with mice injected with OVA loaded microparticles. A similar trend was seen in mice immunised with OVA loaded microparticles decorated with CpG or solutions of admixed OVA and CpG (P<0.05). These data demonstrate, for the first time, that appropriately formulated ssRNA can act as a potent adjuvant and modulator of adaptive immunological responses.
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short communication immunological responses after immunisation of mice with microparticles containing antigen and single stranded rna Polyuridylic Acid
2006Co-Authors: Angie Westwood, Stephen J Elvin, Gareth D Healey, Diane E Williamson, James Edward EylesAbstract:Abstract Certain toll-like receptor (TLR) agonists, e.g. CpG DNA, can be used as potent vaccine ‘adjuvants’. It is known that some sequences ofsingle stranded (ss) RNA stimulate proinflammatory and antiviral responses following interaction with TLR 7 and 8. We have encapsulatedovalbumin (OVA) in the presence and absence of Polyuridylic Acid (poly-U) inside polylactide microparticles. In comparison to microparticlescontaining only OVA, bulk cultures of bone marrow-derived plasmacytoid and myeloid dendritic cells produced more ( P <0.05) IL-12 andinterferon(IFN)-alphawhenstimulatedwithmicroparticlescontainingOVAandpoly-U.SubcutaneousinjectionofcomicroencapsulatedOVAand poly-U resulted in statistically elevated levels of serum anti-OVA IgG1 ( P <0.05 versus na¨ive mice). Conversely, anti-OVA IgG1 levelsin C57 BL6 mice immunised with OVA loaded microparticles (without RNA) were statistically indifferent to na¨ive animals. Furthermore,injection of coencapsulated OVA and poly-U resulted in (
Ara G. Hovanessian - One of the best experts on this subject based on the ideXlab platform.
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Antiviral action of polyadenylic-Polyuridylic Acid against HIV in cell cultures.
AIDS research and human retroviruses, 1992Co-Authors: Anne G. Laurent-crawford, Bernard Krust, Evelyn Deschamps De Paillette, Luc Montagnier, Ara G. HovanessianAbstract:Polyadenylic-Polyuridylic Acid referred to as poly(A)·poly(U) is a synthetic double-stranded RNA which has been shown to manifest both antitumoral and immunomodulatory activities. Here we used this...
Angie Westwood - One of the best experts on this subject based on the ideXlab platform.
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immunological responses after immunisation of mice with microparticles containing antigen and single stranded rna Polyuridylic Acid
Vaccine, 2006Co-Authors: Angie Westwood, Stephen J Elvin, Gareth D Healey, Diane E Williamson, James Edward EylesAbstract:Certain toll-like receptor (TLR) agonists, e.g. CpG DNA, can be used as potent vaccine 'adjuvants'. It is known that some sequences of single stranded (ss) RNA stimulate proinflammatory and antiviral responses following interaction with TLR 7 and 8. We have encapsulated ovalbumin (OVA) in the presence and absence of Polyuridylic Acid (poly-U) inside polylactide microparticles. In comparison to microparticles containing only OVA, bulk cultures of bone marrow-derived plasmacytoid and myeloid dendritic cells produced more (P<0.05) IL-12 and interferon (IFN)-alpha when stimulated with microparticles containing OVA and poly-U. Subcutaneous injection of comicroencapsulated OVA and poly-U resulted in statistically elevated levels of serum anti-OVA IgG1 (P<0.05 versus naive mice). Conversely, anti-OVA IgG1 levels in C57 BL6 mice immunised with OVA loaded microparticles (without RNA) were statistically indifferent to naive animals. Furthermore, injection of coencapsulated OVA and poly-U resulted in (P<0.05) greater numbers of OVA specific IFN-gamma secreting T-cells as compared with mice injected with OVA loaded microparticles. A similar trend was seen in mice immunised with OVA loaded microparticles decorated with CpG or solutions of admixed OVA and CpG (P<0.05). These data demonstrate, for the first time, that appropriately formulated ssRNA can act as a potent adjuvant and modulator of adaptive immunological responses.
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short communication immunological responses after immunisation of mice with microparticles containing antigen and single stranded rna Polyuridylic Acid
2006Co-Authors: Angie Westwood, Stephen J Elvin, Gareth D Healey, Diane E Williamson, James Edward EylesAbstract:Abstract Certain toll-like receptor (TLR) agonists, e.g. CpG DNA, can be used as potent vaccine ‘adjuvants’. It is known that some sequences ofsingle stranded (ss) RNA stimulate proinflammatory and antiviral responses following interaction with TLR 7 and 8. We have encapsulatedovalbumin (OVA) in the presence and absence of Polyuridylic Acid (poly-U) inside polylactide microparticles. In comparison to microparticlescontaining only OVA, bulk cultures of bone marrow-derived plasmacytoid and myeloid dendritic cells produced more ( P <0.05) IL-12 andinterferon(IFN)-alphawhenstimulatedwithmicroparticlescontainingOVAandpoly-U.SubcutaneousinjectionofcomicroencapsulatedOVAand poly-U resulted in statistically elevated levels of serum anti-OVA IgG1 ( P <0.05 versus na¨ive mice). Conversely, anti-OVA IgG1 levelsin C57 BL6 mice immunised with OVA loaded microparticles (without RNA) were statistically indifferent to na¨ive animals. Furthermore,injection of coencapsulated OVA and poly-U resulted in (