The Experts below are selected from a list of 360 Experts worldwide ranked by ideXlab platform
Electron Kebebew - One of the best experts on this subject based on the ideXlab platform.
-
mir 126 3p inhibits thyroid cancer cell growth and metastasis and is associated with aggressive thyroid cancer
PLOS ONE, 2015Co-Authors: Yin Xiong, Lisa Zhang, Martha A Zeiger, Shweta Kotian, Electron KebebewAbstract:Background Previous studies have shown that microRNAs are dysregulated in thyroid cancer and play important roles in the Post-Transcriptional Regulation of target oncogenes and/or tumor suppressor genes.
Horace H Loh - One of the best experts on this subject based on the ideXlab platform.
-
post transcriptional Regulation of mu opioid receptor role of the rna binding proteins heterogeneous nuclear ribonucleoprotein h1 and f
Cellular and Molecular Life Sciences, 2012Co-Authors: Kyu Young Song, Hack Sun Choi, Pingyee Law, Lina Wei, Horace H LohAbstract:Classical opioids have been historically used for the treatment of pain and are among the most widely used drugs for both acute severe pain and long-term pain. Morphine and endogenous mu-opioid peptides exert their pharmacological actions mainly through the mu-opioid receptor (MOR). However, the expression of opioid receptor (OR) proteins is controlled by extensive transcriptional and Post-Transcriptional processing. Previously, the 5′-untranslated region (UTR) of the mouse MOR was found to be important for Post-Transcriptional Regulation of the MOR gene in neuronal cells. To identify proteins binding to the 5′-UTR as potential regulators of the mouse MOR gene, affinity column chromatography using 5′-UTR-specific RNA oligonucleotides was performed using neuroblastoma NS20Y cells. Chromatography was followed by two-dimensional gel electrophoresis and MALDI-TOF mass spectrometry. We identified two heterogeneous ribonucleoproteins (hnRNPs) that bound to RNA sequences of interest: hnRNP H1 and hnRNP F. Binding of these proteins to the RNA region was M4-region sequence-specific as confirmed by Western-blot analysis and RNA supershift assay. Furthermore, a cotransfection study showed that the presence of hnRNP H1 and F resulted in repressed expression of the mouse MOR. Our data suggest that hnRNP H1 and F can function as repressors of MOR translation dependent on the M4 (−75 to −71 bp upstream of ATG) sequences. We demonstrate for the first time a role of hnRNPs as Post-Transcriptional repressors in MOR gene Regulation.
Alessandro Vasciaveo - One of the best experts on this subject based on the ideXlab platform.
-
an extended gene protein products boolean network model including post transcriptional Regulation
Theoretical Biology and Medical Modelling, 2014Co-Authors: Alfredo Benso, Stefano Di Carlo, Gianfranco Politano, Alessandro Savino, Alessandro VasciaveoAbstract:Background Networks Biology allows the study of complex interactions between biological systems using formal, well structured, and computationally friendly models. Several different network models can be created, depending on the type of interactions that need to be investigated. Gene Regulatory Networks (GRN) are an effective model commonly used to study the complex regulatory mechanisms of a cell. Unfortunately, given their intrinsic complexity and non discrete nature, the computational study of realistic-sized complex GRNs requires some abstractions. Boolean Networks (BNs), for example, are a reliable model that can be used to represent networks where the possible state of a node is a boolean value (0 or 1). Despite this strong simplification, BNs have been used to study both structural and dynamic properties of real as well as randomly generated GRNs.
-
using boolean networks to model post transcriptional Regulation in gene regulatory networks
Journal of Computational Science, 2014Co-Authors: Gianfranco Politano, Alfredo Benso, Stefano Di Carlo, Alessandro Savino, Hafeez Ur Rehman, Alessandro VasciaveoAbstract:Abstract Gene regulatory networks (GRNs) model some of the mechanisms that regulate gene expression. Among the computational approaches available to model and study GNRs, Boolean network (BN) emerged as very successful to better understand both the structural and dynamical properties of GRNs. Nevertheless, the most widely used models based on BNs do not include any Post-Transcriptional Regulation mechanism. Since miRNAs have been proved to play an important regulatory role, in this paper we show how the Post-Transcriptional Regulation mechanism mediated by miRNAs has been included in an enhanced BN-based model. We resort to the miR-7 in two Drosophila cell fate determination networks to verify the effectiveness of miRNAs modeling in BNs, by implementing it in our tool for the analysis of Boolean networks.
Gaëtan Droc - One of the best experts on this subject based on the ideXlab platform.
-
Post-Transcriptional Regulation of several biological processes involved in latex production in Hevea brasiliensis
PeerJ, 2020Co-Authors: Julie Leclercq, Benoît Farinas, Stéphanie Pointet, Bénédicte Favreau, Hélène Vignes, Kuswanhadi Kuswanhadi, Enrique Ortega-abboud, Jean-françois Dufayard, Shenghan Gao, Gaëtan DrocAbstract:Background. Small RNAs modulate plant gene expression at both the transcriptional and Post-Transcriptional level, mostly through the induction of either targeted DNA methylation or transcript cleavage, respectively. Small RNA networks are involved in specific plant developmental processes, in signaling pathways triggered by various abiotic stresses and in interactions between the plant and viral and non-viral pathogens. They are also involved in silencing maintenance of transposable elements and endogenous viral elements. Alteration in small RNA production in response to various environmental stresses can affect all the above-mentioned processes. In rubber trees, changes observed in small RNA populations in response to trees affected by tapping panel dryness, in comparison to healthy ones, suggest a shift from a transcriptional to a Post-Transcriptional regulatory pathway. This is the first attempt to characterise small RNAs involved in Post-Transcriptional silencing and their target transcripts in Hevea. Methods. Genes producing microRNAs (MIR genes) and loci producing trans-activated small interfering RNA (ta-siRNA) were identified in the clone PB 260 re-sequenced genome. Degradome libraries were constructed with a pool of total RNA from six different Hevea tissues in stressed and non-stressed plants. The analysis of cleaved RNA data, associated with genomics and transcriptomics data, led to the identification of transcripts that are affected by 20-22 nt small RNA-mediated Post-Transcriptional Regulation. A detailed analysis was carried out on gene families related to latex production and in response to growth regulators. Results. Compared to other tissues, latex cells had a higher proportion of transcript cleavage activity mediated by miRNAs and ta-siRNAs. Post-Transcriptional Regulation was also observed at each step of the natural rubber biosynthesis pathway. Among the genes involved in the miRNA biogenesis pathway, our analyses showed that all of them are expressed in latex. Using phylogenetic analyses, we show that both the Argonaute and Dicer-like gene families recently underwent expansion. Overall, our study underlines the fact that important biological pathways, including hormonal signalling and rubber biosynthesis, are subject to Post-Transcriptional silencing in laticifers.
Susan Gottesman - One of the best experts on this subject based on the ideXlab platform.
-
complex transcriptional and post transcriptional Regulation of an enzyme for lipopolysaccharide modification
Molecular Microbiology, 2013Co-Authors: Kyung Moon, David A Six, Hyunjung Lee, Christian R H Raetz, Susan GottesmanAbstract:Summary The PhoQ/PhoP two-component system activates many genes for lipopolysaccharide (LPS) modification when cells are grown at low Mg2+ concentrations. An additional target of PhoQ and PhoP is MgrR, an Hfq-dependent small RNA that negatively regulates expression of eptB, also encoding a protein that carries out LPS modification. Examination of LPS confirmed that MgrR effectively silences EptB; the phosphoethanolamine modification associated with EptB is found in ΔmgrR::kan but not mgrR+ cells. Sigma E has been reported to positively regulate eptB, although the eptB promoter does not have the expected Sigma E recognition motifs. The effects of Sigma E and deletion of mgrR on levels of eptB mRNA were independent, and the same 5′ end was found in both cases. In vitro transcription and the behaviour of transcriptional and translational fusions demonstrate that Sigma E acts directly at the level of transcription initiation for eptB, from the same start point as Sigma 70. The results suggest that when Sigma E is active, synthesis of eptB transcript outstrips MgrR-dependent degradation; presumably the modification of LPS is important under these conditions. Adding to the complexity of eptB Regulation is a second sRNA, ArcZ, which also directly and negatively regulates eptB.
-
involvement of a novel transcriptional activator and small rna in post transcriptional Regulation of the glucose phosphoenolpyruvate phosphotransferase system
Molecular Microbiology, 2004Co-Authors: Carin K Vanderpool, Susan GottesmanAbstract:: RyaA is a small non-coding RNA in Escherichia coli that was identified by its ability to bind tightly to the RNA chaperone Hfq. This study reports the role of RyaA in mediating the cellular response to glucose-specific phosphoenolypyruvate phosphotransferase system (PTS)-dependent phosphosugar stress. Aiba and co-workers have shown that a block in the metabolism of glucose 6-phosphate causes transient growth inhibition and Post-Transcriptional Regulation of ptsG, encoding the glucose-specific PTS transporter. We found that RyaA synthesis was induced by a non-metabolizable glucose phosphate analogue and was necessary for relief of the toxicity of glucose phosphate stress. Expression of RyaA was sufficient to cause a rapid loss of ptsG mRNA, probably reflecting degradation of the message mediated by RyaA:ptsG pairing. The ryaA gene was renamed sgrS, for sugar transport-related sRNA. Expression of sgrS is regulated by a novel transcriptional activator, SgrR (formerly YabN), which has a putative DNA-binding domain and a solute-binding domain similar to those found in certain transport proteins. Our results suggest that under conditions of glucose phosphate accumulation, SgrR activates SgrS synthesis, causing degradation of ptsG mRNA. Decreased ptsG mRNA results in decreased production of glucose transport machinery, thus limiting further accumulation of glucose phosphate.
