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M.c. Biol-n'garagba - One of the best experts on this subject based on the ideXlab platform.
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Expression of galectin 4 in the rat small intestine during Postnatal Development
Biochimie, 2004Co-Authors: E. Niepceron, F. Simian, P. Louisot, M.c. Biol-n'garagbaAbstract:We determined the expression of an endogenous lectin, galectin 4, in the rat small intestine during Postnatal Development. The mRNA levels of galectin 4 did not change significantly between birth and adulthood. In contrast, the protein was present at higher levels after than before weaning, and the potential ligands for galectin 4 were more highly represented in the enterocyte microvilli of weaned than of suckling rats. These results suggest possible differences either in galectin 4 mRNA stability, in its translation regulation or in the protein stability
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Expression and localization of galectin 4 in rat stomach during Postnatal Development
International Journal of Biochemistry and Cell Biology, 2004Co-Authors: E. Niepceron, P. Louisot, F. Simian-lerme, M.c. Biol-n'garagbaAbstract:Galectins are lectins implicated in cell-cell or cell-matrix adhesion, cell growth, the cell cycle, transcription processes, and apoptosis, and some of them are differentially regulated during pre- or post-natal Development. The purpose of the present study was to determine whether the expression of galectin 4 is relevant to Developmental processes during Postnatal Development in the rat stomach. Galectin 4 expression in the rat gastric mucosa, between birth and adulthood, was studied at the protein and mRNA levels by western and northern blotting, respectively. This lectin was localized precisely by immunoelectron microscopy. In the gastric mucosa, galectin 4 protein was present at lower levels in suckling than in weaned rats, but mRNA levels did not change significantly during Postnatal Development. This suggests possible differences in mRNA stability or in the translation regulation. Immunocytochemical examination of galectin 4 confirmed more highly elevated levels of the protein in endocrine, parietal, and chief cells in weaned rats than in suckling rats. Galectin 4 was more strongly localized in weaned rats than in suckling rats in the nuclei of all cell types and in or over secretory granules in endocrine and chief cells, suggesting that galectin 4 is implicated in nuclear events and perhaps in secretory processes.
Allan E Herbison - One of the best experts on this subject based on the ideXlab platform.
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Postnatal Development of kisspeptin neurons in mouse hypothalamus sexual dimorphism and projections to gonadotropin releasing hormone neurons
Endocrinology, 2006Co-Authors: Jenny Clarkson, Allan E HerbisonAbstract:The neuropeptide kisspeptin has recently been implicated as having a critical role in the activation of the GnRH neurons to bring about puberty. We examined here the Postnatal Development of kisspeptin neuronal populations and their projections to GnRH neurons in the mouse. Three populations of kisspeptin neurons located in the 1) anteroventral periventricular nucleus (AVPV) and the preoptic periventricular nucleus (PeN), 2) dorsomedial hypothalamus, and 3) arcuate nucleus were identified using an antisera raised against mouse kisspeptin-10. A marked 10-fold (P < 0.01), female-dominant sex difference in the numbers of kisspeptin neurons existed in the AVPV/PeN but not elsewhere. Kisspeptin neurons in the AVPV/PeN of both sexes displayed a similar pattern of Postnatal Development with no cells detected at Postnatal day (P) 10, followed by increases from P25 to reach adult levels by puberty onset (P < 0.01; P31 females and P45 males). This pattern was not found in the dorsomedial hypothalamus or arcuate nuc...
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Postnatal Development of kisspeptin neurons in mouse hypothalamus sexual dimorphism and projections to gonadotropin releasing hormone neurons
Endocrinology, 2006Co-Authors: Jenny Clarkson, Allan E HerbisonAbstract:The neuropeptide kisspeptin has recently been implicated as having a critical role in the activation of the GnRH neurons to bring about puberty. We examined here the Postnatal Development of kisspeptin neuronal populations and their projections to GnRH neurons in the mouse. Three populations of kisspeptin neurons located in the 1) anteroventral periventricular nucleus (AVPV) and the preoptic periventricular nucleus (PeN), 2) dorsomedial hypothalamus, and 3) arcuate nucleus were identified using an antisera raised against mouse kisspeptin-10. A marked 10-fold (P<0.01), female-dominant sex difference in the numbers of kisspeptin neurons existed in the AVPV/PeN but not elsewhere. Kisspeptin neurons in the AVPV/PeN of both sexes displayed a similar pattern of Postnatal Development with no cells detected at Postnatal day (P) 10, followed by increases from P25 to reach adult levels by puberty onset (P<0.01; P31 females and P45 males). This pattern was not found in the dorsomedial hypothalamus or arcuate nucleus. Dual immunofluorescence experiments demonstrated close appositions between kisspeptin fibers and GnRH neuron cell bodies that were first apparent at P25 and increased across Postnatal Development in both sexes. These studies demonstrate kisspeptin peptide expression in the mouse hypothalamus and reveal the Postnatal Development of a sexually dimorphic continuum of kisspeptin neurons within the AVPV and PeN. This periventricular population of kisspeptin neurons reaches adult-like proportions at the time of puberty onset and is the likely source of the kisspeptin inputs to GnRH neurons.
E. Niepceron - One of the best experts on this subject based on the ideXlab platform.
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Expression of galectin 4 in the rat small intestine during Postnatal Development
Biochimie, 2004Co-Authors: E. Niepceron, F. Simian, P. Louisot, M.c. Biol-n'garagbaAbstract:We determined the expression of an endogenous lectin, galectin 4, in the rat small intestine during Postnatal Development. The mRNA levels of galectin 4 did not change significantly between birth and adulthood. In contrast, the protein was present at higher levels after than before weaning, and the potential ligands for galectin 4 were more highly represented in the enterocyte microvilli of weaned than of suckling rats. These results suggest possible differences either in galectin 4 mRNA stability, in its translation regulation or in the protein stability
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Expression and localization of galectin 4 in rat stomach during Postnatal Development
International Journal of Biochemistry and Cell Biology, 2004Co-Authors: E. Niepceron, P. Louisot, F. Simian-lerme, M.c. Biol-n'garagbaAbstract:Galectins are lectins implicated in cell-cell or cell-matrix adhesion, cell growth, the cell cycle, transcription processes, and apoptosis, and some of them are differentially regulated during pre- or post-natal Development. The purpose of the present study was to determine whether the expression of galectin 4 is relevant to Developmental processes during Postnatal Development in the rat stomach. Galectin 4 expression in the rat gastric mucosa, between birth and adulthood, was studied at the protein and mRNA levels by western and northern blotting, respectively. This lectin was localized precisely by immunoelectron microscopy. In the gastric mucosa, galectin 4 protein was present at lower levels in suckling than in weaned rats, but mRNA levels did not change significantly during Postnatal Development. This suggests possible differences in mRNA stability or in the translation regulation. Immunocytochemical examination of galectin 4 confirmed more highly elevated levels of the protein in endocrine, parietal, and chief cells in weaned rats than in suckling rats. Galectin 4 was more strongly localized in weaned rats than in suckling rats in the nuclei of all cell types and in or over secretory granules in endocrine and chief cells, suggesting that galectin 4 is implicated in nuclear events and perhaps in secretory processes.
Jenny Clarkson - One of the best experts on this subject based on the ideXlab platform.
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Postnatal Development of kisspeptin neurons in mouse hypothalamus sexual dimorphism and projections to gonadotropin releasing hormone neurons
Endocrinology, 2006Co-Authors: Jenny Clarkson, Allan E HerbisonAbstract:The neuropeptide kisspeptin has recently been implicated as having a critical role in the activation of the GnRH neurons to bring about puberty. We examined here the Postnatal Development of kisspeptin neuronal populations and their projections to GnRH neurons in the mouse. Three populations of kisspeptin neurons located in the 1) anteroventral periventricular nucleus (AVPV) and the preoptic periventricular nucleus (PeN), 2) dorsomedial hypothalamus, and 3) arcuate nucleus were identified using an antisera raised against mouse kisspeptin-10. A marked 10-fold (P < 0.01), female-dominant sex difference in the numbers of kisspeptin neurons existed in the AVPV/PeN but not elsewhere. Kisspeptin neurons in the AVPV/PeN of both sexes displayed a similar pattern of Postnatal Development with no cells detected at Postnatal day (P) 10, followed by increases from P25 to reach adult levels by puberty onset (P < 0.01; P31 females and P45 males). This pattern was not found in the dorsomedial hypothalamus or arcuate nuc...
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Postnatal Development of kisspeptin neurons in mouse hypothalamus sexual dimorphism and projections to gonadotropin releasing hormone neurons
Endocrinology, 2006Co-Authors: Jenny Clarkson, Allan E HerbisonAbstract:The neuropeptide kisspeptin has recently been implicated as having a critical role in the activation of the GnRH neurons to bring about puberty. We examined here the Postnatal Development of kisspeptin neuronal populations and their projections to GnRH neurons in the mouse. Three populations of kisspeptin neurons located in the 1) anteroventral periventricular nucleus (AVPV) and the preoptic periventricular nucleus (PeN), 2) dorsomedial hypothalamus, and 3) arcuate nucleus were identified using an antisera raised against mouse kisspeptin-10. A marked 10-fold (P<0.01), female-dominant sex difference in the numbers of kisspeptin neurons existed in the AVPV/PeN but not elsewhere. Kisspeptin neurons in the AVPV/PeN of both sexes displayed a similar pattern of Postnatal Development with no cells detected at Postnatal day (P) 10, followed by increases from P25 to reach adult levels by puberty onset (P<0.01; P31 females and P45 males). This pattern was not found in the dorsomedial hypothalamus or arcuate nucleus. Dual immunofluorescence experiments demonstrated close appositions between kisspeptin fibers and GnRH neuron cell bodies that were first apparent at P25 and increased across Postnatal Development in both sexes. These studies demonstrate kisspeptin peptide expression in the mouse hypothalamus and reveal the Postnatal Development of a sexually dimorphic continuum of kisspeptin neurons within the AVPV and PeN. This periventricular population of kisspeptin neurons reaches adult-like proportions at the time of puberty onset and is the likely source of the kisspeptin inputs to GnRH neurons.
Bernardo Morenolopez - One of the best experts on this subject based on the ideXlab platform.
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nitric oxide controls excitatory inhibitory balance in the hypoglossal nucleus during early Postnatal Development
Brain Structure & Function, 2020Co-Authors: Federico Portillo, Bernardo MorenolopezAbstract:Synaptic remodeling during early Postnatal Development lies behind neuronal networks refinement and nervous system maturation. In particular, the respiratory system is immature at birth and is subjected to significant Postnatal Development. In this context, the excitatory/inhibitory balance dramatically changes in the respiratory-related hypoglossal nucleus (HN) during the 3 perinatal weeks. Since, Development abnormalities of hypoglossal motor neurons (HMNs) are associated with sudden infant death syndrome and obstructive sleep apnea, deciphering molecular partners behind synaptic remodeling in the HN is of basic and clinical relevance. Interestingly, a transient expression of the neuronal isoform of nitric oxide (NO) synthase (NOS) occurs in HMNs at neonatal stage that disappears before Postnatal day 21 (P21). NO, in turn, is a determining factor for synaptic refinement in several physiopathological conditions. Here, intracerebroventricular chronic administration (P7-P21) of the broad spectrum NOS inhibitor L-NAME (N(ω)-nitro-L-arginine methyl ester) differentially affected excitatory and inhibitory rearrangement during this neonatal interval in the rat. Whilst L-NAME led to a reduction in the number of excitatory structures, inhibitory synaptic puncta were increased at P21 in comparison to administration of the inactive stereoisomer D-NAME. Finally, L-NAME decreased levels of the phosphorylated form of myosin light chain in the nucleus, which is known to regulate the actomyosin contraction apparatus. These outcomes indicate that physiologically synthesized NO modulates excitatory/inhibitory balance during early Postnatal Development by acting as an anti-synaptotrophic and/or synaptotoxic factor for inhibitory synapses, and as a synaptotrophin for excitatory ones. The mechanism of action could rely on the modulation of the actomyosin contraction apparatus.
