The Experts below are selected from a list of 24225 Experts worldwide ranked by ideXlab platform
Isao Makino - One of the best experts on this subject based on the ideXlab platform.
-
restoration of the glucocorticoid receptor function by the phosphodiester compound of vitamins c and e epc k1 l ascorbic acid 2 3 4 dihydro 2 5 7 8 tetramethyl 2 4 8 12 trimethyltridecyl 2h 1 benzopyran 6 yl hydrogen phosphate Potassium Salt via a redox dependent mechanism
Biochemical Pharmacology, 1998Co-Authors: Kensaku Okamoto, Hirotoshi Tanaka, Yuichi Makino, Isao MakinoAbstract:Abstract We examined the effect of the novel antioxidant EPC-K1 ( l -ascorbic acid 2-[3,4-dihydro-2,5,7,8-tetramethyl-2-(4,8,12-trimethyltridecyl)-2H-1-benzopyran-6-yl hydrogen phosphate] Potassium Salt) on glucocorticoid receptor function. We used cloned CHOpMTGR cells in which human glucocorticoid receptor cDNA was stably transfected and the glucocorticoid receptor was expressed at high levels. We recently suggested that glucocorticoid-mediated gene expression is modulated via the cellular redox state [Makino et al., J Clin Invest98: 2469–2477, 1996]. In the present study, this issue was clearly evidenced by the finding that cellular treatment with H2O2 decreased the ligand binding and transcriptional activity of the glucocorticoid receptor, and we showed that these inhibitory effects of H2O2 were effectively titrated by the addition of EPC-K1. Moreover, DNA-binding activity of the bacterially expressed DNA-binding domain of the glucocorticoid receptor was repressed by the thiol-oxidizing reagent diamide; EPC-K1 also counteracted this repressive effect of diamide. Thus, the redox state was indicated to influence glucocorticoid receptor function at various steps, and EPC-K1 may be useful in restoring the cellular glucocorticoid-responsiveness in oxidative conditions.
Kensaku Okamoto - One of the best experts on this subject based on the ideXlab platform.
-
restoration of the glucocorticoid receptor function by the phosphodiester compound of vitamins c and e epc k1 l ascorbic acid 2 3 4 dihydro 2 5 7 8 tetramethyl 2 4 8 12 trimethyltridecyl 2h 1 benzopyran 6 yl hydrogen phosphate Potassium Salt via a redox dependent mechanism
Biochemical Pharmacology, 1998Co-Authors: Kensaku Okamoto, Hirotoshi Tanaka, Yuichi Makino, Isao MakinoAbstract:Abstract We examined the effect of the novel antioxidant EPC-K1 ( l -ascorbic acid 2-[3,4-dihydro-2,5,7,8-tetramethyl-2-(4,8,12-trimethyltridecyl)-2H-1-benzopyran-6-yl hydrogen phosphate] Potassium Salt) on glucocorticoid receptor function. We used cloned CHOpMTGR cells in which human glucocorticoid receptor cDNA was stably transfected and the glucocorticoid receptor was expressed at high levels. We recently suggested that glucocorticoid-mediated gene expression is modulated via the cellular redox state [Makino et al., J Clin Invest98: 2469–2477, 1996]. In the present study, this issue was clearly evidenced by the finding that cellular treatment with H2O2 decreased the ligand binding and transcriptional activity of the glucocorticoid receptor, and we showed that these inhibitory effects of H2O2 were effectively titrated by the addition of EPC-K1. Moreover, DNA-binding activity of the bacterially expressed DNA-binding domain of the glucocorticoid receptor was repressed by the thiol-oxidizing reagent diamide; EPC-K1 also counteracted this repressive effect of diamide. Thus, the redox state was indicated to influence glucocorticoid receptor function at various steps, and EPC-K1 may be useful in restoring the cellular glucocorticoid-responsiveness in oxidative conditions.
P Srinivas - One of the best experts on this subject based on the ideXlab platform.
-
ultrasound assisted reaction of 2 3 4 6 tetra o acetyl α d glucopyranosyl bromide with Potassium Salt of curcumin under ptc conditions
Ultrasonics Sonochemistry, 2008Co-Authors: Kattera S Parvathy, P SrinivasAbstract:Reaction of 2,3,4,6-tetra-O-acetyl-alpha-D-glucopyranosyl bromide with Potassium Salt of curcumin [bis-1,7-(3'-methoxy-4'-hydroxy)phenyl-5-hydroxy-1,4,6-heptatrien-3-one] under either thermal or high pressure conditions affect the labile substrate, curcumin, thus resulting in drastic reduction in the yields of the glucosides. This drawback could be effectively overcome by carrying out the biphasic reaction in the presence of a phase transfer catalyst under the effect of ultrasound. The reaction under the sonochemical conditions was faster and resulted in the increased yield of the glucoside products. The reaction was investigated in detail with a view to optimizing the yield of the glucosides. The detailed study clearly indicated the important role of the nature and quantity of the phase transfer catalyst employed in the reaction. Also, the selectivity with respect to the formation of mono- or di-beta-glucosides under both mono- and biphasic reaction conditions was clearly discernable. The study establishes a simple synthetic protocol for the glucoside derivatives of curcumin in high yields and selectivity using ultrasonic waves.
-
ultrasound assisted reaction of 2 3 4 6 tetra o acetyl α d glucopyranosyl bromide with Potassium Salt of curcumin under ptc conditions
Ultrasonics Sonochemistry, 2008Co-Authors: Kattera S Parvathy, P SrinivasAbstract:Reaction of 2,3,4,6-tetra-O-acetyl-α-d-glucopyranosyl bromide with Potassium Salt of curcumin [bis-1,7-(3′-methoxy-4′-hydroxy)phenyl-5-hydroxy-1,4,6-heptatrien-3-one] under either thermal or high pressure conditions affect the labile substrate, curcumin, thus resulting in drastic reduction in the yields of the glucosides. This drawback could be effectively overcome by carrying out the biphasic reaction in the presence of a phase transfer catalyst under the effect of ultrasound. The reaction under the sonochemical conditions was faster and resulted in the increased yield of the glucoside products. The reaction was investigated in detail with a view to optimizing the yield of the glucosides. The detailed study clearly indicated the important role of the nature and quantity of the phase transfer catalyst employed in the reaction. Also, the selectivity with respect to the formation of mono- or di-β-glucosides under both mono- and biphasic reaction conditions was clearly discernable. The study establishes a simple synthetic protocol for the glucoside derivatives of curcumin in high yields and selectivity using ultrasonic waves.
Hirotoshi Tanaka - One of the best experts on this subject based on the ideXlab platform.
-
restoration of the glucocorticoid receptor function by the phosphodiester compound of vitamins c and e epc k1 l ascorbic acid 2 3 4 dihydro 2 5 7 8 tetramethyl 2 4 8 12 trimethyltridecyl 2h 1 benzopyran 6 yl hydrogen phosphate Potassium Salt via a redox dependent mechanism
Biochemical Pharmacology, 1998Co-Authors: Kensaku Okamoto, Hirotoshi Tanaka, Yuichi Makino, Isao MakinoAbstract:Abstract We examined the effect of the novel antioxidant EPC-K1 ( l -ascorbic acid 2-[3,4-dihydro-2,5,7,8-tetramethyl-2-(4,8,12-trimethyltridecyl)-2H-1-benzopyran-6-yl hydrogen phosphate] Potassium Salt) on glucocorticoid receptor function. We used cloned CHOpMTGR cells in which human glucocorticoid receptor cDNA was stably transfected and the glucocorticoid receptor was expressed at high levels. We recently suggested that glucocorticoid-mediated gene expression is modulated via the cellular redox state [Makino et al., J Clin Invest98: 2469–2477, 1996]. In the present study, this issue was clearly evidenced by the finding that cellular treatment with H2O2 decreased the ligand binding and transcriptional activity of the glucocorticoid receptor, and we showed that these inhibitory effects of H2O2 were effectively titrated by the addition of EPC-K1. Moreover, DNA-binding activity of the bacterially expressed DNA-binding domain of the glucocorticoid receptor was repressed by the thiol-oxidizing reagent diamide; EPC-K1 also counteracted this repressive effect of diamide. Thus, the redox state was indicated to influence glucocorticoid receptor function at various steps, and EPC-K1 may be useful in restoring the cellular glucocorticoid-responsiveness in oxidative conditions.
Yuichi Makino - One of the best experts on this subject based on the ideXlab platform.
-
restoration of the glucocorticoid receptor function by the phosphodiester compound of vitamins c and e epc k1 l ascorbic acid 2 3 4 dihydro 2 5 7 8 tetramethyl 2 4 8 12 trimethyltridecyl 2h 1 benzopyran 6 yl hydrogen phosphate Potassium Salt via a redox dependent mechanism
Biochemical Pharmacology, 1998Co-Authors: Kensaku Okamoto, Hirotoshi Tanaka, Yuichi Makino, Isao MakinoAbstract:Abstract We examined the effect of the novel antioxidant EPC-K1 ( l -ascorbic acid 2-[3,4-dihydro-2,5,7,8-tetramethyl-2-(4,8,12-trimethyltridecyl)-2H-1-benzopyran-6-yl hydrogen phosphate] Potassium Salt) on glucocorticoid receptor function. We used cloned CHOpMTGR cells in which human glucocorticoid receptor cDNA was stably transfected and the glucocorticoid receptor was expressed at high levels. We recently suggested that glucocorticoid-mediated gene expression is modulated via the cellular redox state [Makino et al., J Clin Invest98: 2469–2477, 1996]. In the present study, this issue was clearly evidenced by the finding that cellular treatment with H2O2 decreased the ligand binding and transcriptional activity of the glucocorticoid receptor, and we showed that these inhibitory effects of H2O2 were effectively titrated by the addition of EPC-K1. Moreover, DNA-binding activity of the bacterially expressed DNA-binding domain of the glucocorticoid receptor was repressed by the thiol-oxidizing reagent diamide; EPC-K1 also counteracted this repressive effect of diamide. Thus, the redox state was indicated to influence glucocorticoid receptor function at various steps, and EPC-K1 may be useful in restoring the cellular glucocorticoid-responsiveness in oxidative conditions.