The Experts below are selected from a list of 165 Experts worldwide ranked by ideXlab platform
Samir Attoub - One of the best experts on this subject based on the ideXlab platform.
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Inhibitory Effects of Salinomycin on Cell Survival, Colony Growth, Migration, and Invasion of Human Non-Small Cell Lung Cancer A549 and LNM35: Involvement of NAG-1
2016Co-Authors: Kholoud Arafat, Rabah Iratni, Takashi Takahashi, Khatija Parekh, Yusra Al Dhaheri, Thomas E. Adrian, Samir AttoubAbstract:A major challenge for oncologists and pharmacologists is to develop more potent and less toxic drugs that will decrease the tumor growth and improve the survival of lung cancer patients. Salinomycin is a polyether antibiotic used to kill gram-positive bacteria including mycobacteria, protozoans such as plasmodium falciparum, and the parasites responsible for the Poultry Disease coccidiosis. This old agent is now a serious anti-cancer drug candidate that selectively inhibits the growth of cancer stem cells. We investigated the impact of salinomycin on survival, colony growth, migration and invasion of the differentiated human non-small cell lung cancer lines LNM35 and A549. Salinomycin caused concentration- and time-dependent reduction in viability of LNM35 and A549 cells through a caspase 3/7-associated cell death pathway. Similarly, salinomycin (2.5–5 mM for 7 days) significantly decreased the growth of LNM35 and A549 colonies in soft agar. Metastasis is the main cause of death related to lung cancer. In this context, salinomycin induced a time- and concentration-dependent inhibition of cell migration and invasion. We also demonstrated for the first time that salinomycin induced a marked increase in the expression of the pro-apoptotic protein NAG-1 leading to the inhibition of lung cancer cell invasion but no
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inhibitory effects of salinomycin on cell survival colony growth migration and invasion of human non small cell lung cancer a549 and lnm35 involvement of nag 1
PLOS ONE, 2013Co-Authors: Kholoud Arafat, Rabah Iratni, Takashi Takahashi, Khatija Parekh, Yusra Al Dhaheri, Thomas E. Adrian, Samir AttoubAbstract:A major challenge for oncologists and pharmacologists is to develop more potent and less toxic drugs that will decrease the tumor growth and improve the survival of lung cancer patients. Salinomycin is a polyether antibiotic used to kill gram-positive bacteria including mycobacteria, protozoans such as plasmodium falciparum, and the parasites responsible for the Poultry Disease coccidiosis. This old agent is now a serious anti-cancer drug candidate that selectively inhibits the growth of cancer stem cells. We investigated the impact of salinomycin on survival, colony growth, migration and invasion of the differentiated human non-small cell lung cancer lines LNM35 and A549. Salinomycin caused concentration- and time-dependent reduction in viability of LNM35 and A549 cells through a caspase 3/7-associated cell death pathway. Similarly, salinomycin (2.5–5 µM for 7 days) significantly decreased the growth of LNM35 and A549 colonies in soft agar. Metastasis is the main cause of death related to lung cancer. In this context, salinomycin induced a time- and concentration-dependent inhibition of cell migration and invasion. We also demonstrated for the first time that salinomycin induced a marked increase in the expression of the pro-apoptotic protein NAG-1 leading to the inhibition of lung cancer cell invasion but not cell survival. These findings identify salinomycin as a promising novel therapeutic agent for lung cancer.
Huanmin Zhang - One of the best experts on this subject based on the ideXlab platform.
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a premature stop codon within the tvb receptor gene results in decreased susceptibility to infection by avian leukosis virus subgroups b d and e
Oncotarget, 2017Co-Authors: Weiguo Chen, Xinheng Zhang, Aijun Li, Xinjian Li, Hongxing Li, Huanmin ZhangAbstract:// WeiGuo Chen 1, 5, 6, * , Yang Liu 1, 5, * , Aijun Li 2 , Xinjian Li 1, 5 , Hongxing Li 1, 5 , Zhenkai Dai 1, 5 , Yiming Yan 1, 5 , Xinheng Zhang 1, 5 , Dingming Shu 3 , Huanmin Zhang 4 , Wencheng Lin 1, 5, 6 , Jingyun Ma 1, 5, 6 and Qingmei Xie 1, 5, 6 1 College of Animal Science, South China Agricultural University & Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding, Guangzhou 510642, P. R. China 2 College of Science and Engineering, Jinan University, Guangzhou 510632, P. R. China 3 Institute of Animal Science, Guangdong Academy of Agriculture Sciences, Guangzhou 510640, P. R. China 4 USDA, Agriculture Research Service, Avian Disease and Oncology Laboratory, East Lansing, MI 48823, USA 5 Key Laboratory of Animal Health Aquaculture and Environmental Control, Guangdong, Guangzhou 510642, P. R. China 6 South China Collaborative Innovation Center for Poultry Disease Control and Product Safety, Guangzhou 510642, P. R. China * These authors have contributed equally to this work Correspondence to: Qingmei Xie, email: qmx@scau.edu.cn Keywords: avian leukosis virus; tvb receptor gene; premature stop codon; host resistance; chicken Received: June 15, 2017 Accepted: November 03, 2017 Published: November 18, 2017 ABSTRACT Avian leukosis virus (ALV) is an oncogenic virus causing a variety of neoplasms in chickens. The group of avian leukosis virus in chickens contains six closely related subgroups, A to E and J. The prevalence of ALVs in hosts may have imposed strong selective pressure toward resistance to ALVs infection. The tvb gene encodes Tvb receptor and determines susceptibility or resistance to the subgroups B, D, and E ALV. In this study, we characterized a novel resistant allele of the tvb receptor gene, tvb r3 , which carries a single-nucleotide substitution (c.298C>T) that constitutes a premature termination codon within the fourth exon and leads to the production of a truncated Tvb R3 receptor protein. As a result, we observed decreased susceptibility to infection by ALV-B, ALV-D and ALV-E both in vitro and in vivo , and decreased the binding affinity of the Tvb R3 receptor for the subgroups B, D, and E ALV envelope glycoproteins. Additionally, we found that the tvb r3 allele was prevalent in Chinese broiler lines. This study demonstrated that premature termination codon in the tvb receptor gene can confer genetic resistance to subgroups B, D, and E ALV in the host, and indicates that tvb r3 could potentially serve as a resistant target against ALV-B, ALV-D and ALV-E infection.
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circular rna alterations are involved in resistance to avian leukosis virus subgroup j induced tumor formation in chickens
Oncotarget, 2017Co-Authors: Xinheng Zhang, Aijun Li, Huanmin Zhang, Xinjian Li, Weiguo Chen, Feng ChenAbstract:// Xinheng Zhang 1, 2, 5, 6 , Yiming Yan 1, 2, 5 , Xiaoya Lei 1, 2, 5 , Aijun Li 3 , Huanmin Zhang 4 , Zhenkai Dai 1, 2, 5 , Xinjian Li 1, 2, 5 , Weiguo Chen 1, 2, 5, 6 , Wencheng Lin 1, 2, 5, 6 , Feng Chen 1, 2, 5, 6 , Jingyun Ma 1, 2, 5, 6 and Qingmei Xie 1, 2, 5, 6 1 College of Animal Science, South China Agricultural University, Guangzhou, 510642, P.R. China 2 Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding, Guangzhou, 510642, P.R. China 3 College of Science and Engineering, Jinan University, Guangzhou, 510632, P.R. China 4 USDA, Agriculture Research Service, Avian Disease and Oncology Laboratory, East Lansing, MI, 48823, USA 5 Key Laboratory of Animal Health Aquaculture and Environmental Control, Guangdong, Guangzhou, 510642, P.R. China 6 South China Collaborative Innovation Center for Poultry Disease Control and Product Safety, Guangzhou, 510642, P.R. China Correspondence to: Qingmei Xie, email: qmx@scau.edu.cn Keywords: circular RNA, ALV-J-resistant chickens, circular-seq, tumor Received: November 25, 2016 Accepted: March 08, 2017 Published: March 22, 2017 ABSTRACT Avian leukosis virus subgroup (ALV-J) is an oncogenic neoplasm-inducing retrovirus that causes significant economic losses in the Poultry industry. Recent studies have demonstrated circular RNAs (circRNAs) are implicated in pathogenic processes; however, no research has indicated circRNAs are involved in resistance to Disease. In this study, over 1800 circRNAs were detected by circRNA sequencing of liver tissues from ALV-J-resistant ( n = 3) and ALV-J-susceptible chickens ( n = 3). 32 differentially expressed circRNAs were selected for analyzing including 12 upregulated in ALV-J-resistant chickens and 20 upregulated in ALV-J-susceptible chickens, besides, the top five microRNAs (miRNAs) for 12 upregulated circRNAs in ALV-J-resistant chickens were analyzed. Gene ontology and KEGG pathway analyses were performed for miRNA target genes, the predicted genes were mainly involved in immune pathways. This study provides the first evidence that circRNA alterations are involved in resistance to ALV-J-induced tumor formation. We propose circRNAs may help to mediate tumor induction and development in chickens.
Kholoud Arafat - One of the best experts on this subject based on the ideXlab platform.
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Inhibitory Effects of Salinomycin on Cell Survival, Colony Growth, Migration, and Invasion of Human Non-Small Cell Lung Cancer A549 and LNM35: Involvement of NAG-1
2016Co-Authors: Kholoud Arafat, Rabah Iratni, Takashi Takahashi, Khatija Parekh, Yusra Al Dhaheri, Thomas E. Adrian, Samir AttoubAbstract:A major challenge for oncologists and pharmacologists is to develop more potent and less toxic drugs that will decrease the tumor growth and improve the survival of lung cancer patients. Salinomycin is a polyether antibiotic used to kill gram-positive bacteria including mycobacteria, protozoans such as plasmodium falciparum, and the parasites responsible for the Poultry Disease coccidiosis. This old agent is now a serious anti-cancer drug candidate that selectively inhibits the growth of cancer stem cells. We investigated the impact of salinomycin on survival, colony growth, migration and invasion of the differentiated human non-small cell lung cancer lines LNM35 and A549. Salinomycin caused concentration- and time-dependent reduction in viability of LNM35 and A549 cells through a caspase 3/7-associated cell death pathway. Similarly, salinomycin (2.5–5 mM for 7 days) significantly decreased the growth of LNM35 and A549 colonies in soft agar. Metastasis is the main cause of death related to lung cancer. In this context, salinomycin induced a time- and concentration-dependent inhibition of cell migration and invasion. We also demonstrated for the first time that salinomycin induced a marked increase in the expression of the pro-apoptotic protein NAG-1 leading to the inhibition of lung cancer cell invasion but no
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inhibitory effects of salinomycin on cell survival colony growth migration and invasion of human non small cell lung cancer a549 and lnm35 involvement of nag 1
PLOS ONE, 2013Co-Authors: Kholoud Arafat, Rabah Iratni, Takashi Takahashi, Khatija Parekh, Yusra Al Dhaheri, Thomas E. Adrian, Samir AttoubAbstract:A major challenge for oncologists and pharmacologists is to develop more potent and less toxic drugs that will decrease the tumor growth and improve the survival of lung cancer patients. Salinomycin is a polyether antibiotic used to kill gram-positive bacteria including mycobacteria, protozoans such as plasmodium falciparum, and the parasites responsible for the Poultry Disease coccidiosis. This old agent is now a serious anti-cancer drug candidate that selectively inhibits the growth of cancer stem cells. We investigated the impact of salinomycin on survival, colony growth, migration and invasion of the differentiated human non-small cell lung cancer lines LNM35 and A549. Salinomycin caused concentration- and time-dependent reduction in viability of LNM35 and A549 cells through a caspase 3/7-associated cell death pathway. Similarly, salinomycin (2.5–5 µM for 7 days) significantly decreased the growth of LNM35 and A549 colonies in soft agar. Metastasis is the main cause of death related to lung cancer. In this context, salinomycin induced a time- and concentration-dependent inhibition of cell migration and invasion. We also demonstrated for the first time that salinomycin induced a marked increase in the expression of the pro-apoptotic protein NAG-1 leading to the inhibition of lung cancer cell invasion but not cell survival. These findings identify salinomycin as a promising novel therapeutic agent for lung cancer.
Weiguo Chen - One of the best experts on this subject based on the ideXlab platform.
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a premature stop codon within the tvb receptor gene results in decreased susceptibility to infection by avian leukosis virus subgroups b d and e
Oncotarget, 2017Co-Authors: Weiguo Chen, Xinheng Zhang, Aijun Li, Xinjian Li, Hongxing Li, Huanmin ZhangAbstract:// WeiGuo Chen 1, 5, 6, * , Yang Liu 1, 5, * , Aijun Li 2 , Xinjian Li 1, 5 , Hongxing Li 1, 5 , Zhenkai Dai 1, 5 , Yiming Yan 1, 5 , Xinheng Zhang 1, 5 , Dingming Shu 3 , Huanmin Zhang 4 , Wencheng Lin 1, 5, 6 , Jingyun Ma 1, 5, 6 and Qingmei Xie 1, 5, 6 1 College of Animal Science, South China Agricultural University & Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding, Guangzhou 510642, P. R. China 2 College of Science and Engineering, Jinan University, Guangzhou 510632, P. R. China 3 Institute of Animal Science, Guangdong Academy of Agriculture Sciences, Guangzhou 510640, P. R. China 4 USDA, Agriculture Research Service, Avian Disease and Oncology Laboratory, East Lansing, MI 48823, USA 5 Key Laboratory of Animal Health Aquaculture and Environmental Control, Guangdong, Guangzhou 510642, P. R. China 6 South China Collaborative Innovation Center for Poultry Disease Control and Product Safety, Guangzhou 510642, P. R. China * These authors have contributed equally to this work Correspondence to: Qingmei Xie, email: qmx@scau.edu.cn Keywords: avian leukosis virus; tvb receptor gene; premature stop codon; host resistance; chicken Received: June 15, 2017 Accepted: November 03, 2017 Published: November 18, 2017 ABSTRACT Avian leukosis virus (ALV) is an oncogenic virus causing a variety of neoplasms in chickens. The group of avian leukosis virus in chickens contains six closely related subgroups, A to E and J. The prevalence of ALVs in hosts may have imposed strong selective pressure toward resistance to ALVs infection. The tvb gene encodes Tvb receptor and determines susceptibility or resistance to the subgroups B, D, and E ALV. In this study, we characterized a novel resistant allele of the tvb receptor gene, tvb r3 , which carries a single-nucleotide substitution (c.298C>T) that constitutes a premature termination codon within the fourth exon and leads to the production of a truncated Tvb R3 receptor protein. As a result, we observed decreased susceptibility to infection by ALV-B, ALV-D and ALV-E both in vitro and in vivo , and decreased the binding affinity of the Tvb R3 receptor for the subgroups B, D, and E ALV envelope glycoproteins. Additionally, we found that the tvb r3 allele was prevalent in Chinese broiler lines. This study demonstrated that premature termination codon in the tvb receptor gene can confer genetic resistance to subgroups B, D, and E ALV in the host, and indicates that tvb r3 could potentially serve as a resistant target against ALV-B, ALV-D and ALV-E infection.
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circular rna alterations are involved in resistance to avian leukosis virus subgroup j induced tumor formation in chickens
Oncotarget, 2017Co-Authors: Xinheng Zhang, Aijun Li, Huanmin Zhang, Xinjian Li, Weiguo Chen, Feng ChenAbstract:// Xinheng Zhang 1, 2, 5, 6 , Yiming Yan 1, 2, 5 , Xiaoya Lei 1, 2, 5 , Aijun Li 3 , Huanmin Zhang 4 , Zhenkai Dai 1, 2, 5 , Xinjian Li 1, 2, 5 , Weiguo Chen 1, 2, 5, 6 , Wencheng Lin 1, 2, 5, 6 , Feng Chen 1, 2, 5, 6 , Jingyun Ma 1, 2, 5, 6 and Qingmei Xie 1, 2, 5, 6 1 College of Animal Science, South China Agricultural University, Guangzhou, 510642, P.R. China 2 Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding, Guangzhou, 510642, P.R. China 3 College of Science and Engineering, Jinan University, Guangzhou, 510632, P.R. China 4 USDA, Agriculture Research Service, Avian Disease and Oncology Laboratory, East Lansing, MI, 48823, USA 5 Key Laboratory of Animal Health Aquaculture and Environmental Control, Guangdong, Guangzhou, 510642, P.R. China 6 South China Collaborative Innovation Center for Poultry Disease Control and Product Safety, Guangzhou, 510642, P.R. China Correspondence to: Qingmei Xie, email: qmx@scau.edu.cn Keywords: circular RNA, ALV-J-resistant chickens, circular-seq, tumor Received: November 25, 2016 Accepted: March 08, 2017 Published: March 22, 2017 ABSTRACT Avian leukosis virus subgroup (ALV-J) is an oncogenic neoplasm-inducing retrovirus that causes significant economic losses in the Poultry industry. Recent studies have demonstrated circular RNAs (circRNAs) are implicated in pathogenic processes; however, no research has indicated circRNAs are involved in resistance to Disease. In this study, over 1800 circRNAs were detected by circRNA sequencing of liver tissues from ALV-J-resistant ( n = 3) and ALV-J-susceptible chickens ( n = 3). 32 differentially expressed circRNAs were selected for analyzing including 12 upregulated in ALV-J-resistant chickens and 20 upregulated in ALV-J-susceptible chickens, besides, the top five microRNAs (miRNAs) for 12 upregulated circRNAs in ALV-J-resistant chickens were analyzed. Gene ontology and KEGG pathway analyses were performed for miRNA target genes, the predicted genes were mainly involved in immune pathways. This study provides the first evidence that circRNA alterations are involved in resistance to ALV-J-induced tumor formation. We propose circRNAs may help to mediate tumor induction and development in chickens.
Xinheng Zhang - One of the best experts on this subject based on the ideXlab platform.
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a premature stop codon within the tvb receptor gene results in decreased susceptibility to infection by avian leukosis virus subgroups b d and e
Oncotarget, 2017Co-Authors: Weiguo Chen, Xinheng Zhang, Aijun Li, Xinjian Li, Hongxing Li, Huanmin ZhangAbstract:// WeiGuo Chen 1, 5, 6, * , Yang Liu 1, 5, * , Aijun Li 2 , Xinjian Li 1, 5 , Hongxing Li 1, 5 , Zhenkai Dai 1, 5 , Yiming Yan 1, 5 , Xinheng Zhang 1, 5 , Dingming Shu 3 , Huanmin Zhang 4 , Wencheng Lin 1, 5, 6 , Jingyun Ma 1, 5, 6 and Qingmei Xie 1, 5, 6 1 College of Animal Science, South China Agricultural University & Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding, Guangzhou 510642, P. R. China 2 College of Science and Engineering, Jinan University, Guangzhou 510632, P. R. China 3 Institute of Animal Science, Guangdong Academy of Agriculture Sciences, Guangzhou 510640, P. R. China 4 USDA, Agriculture Research Service, Avian Disease and Oncology Laboratory, East Lansing, MI 48823, USA 5 Key Laboratory of Animal Health Aquaculture and Environmental Control, Guangdong, Guangzhou 510642, P. R. China 6 South China Collaborative Innovation Center for Poultry Disease Control and Product Safety, Guangzhou 510642, P. R. China * These authors have contributed equally to this work Correspondence to: Qingmei Xie, email: qmx@scau.edu.cn Keywords: avian leukosis virus; tvb receptor gene; premature stop codon; host resistance; chicken Received: June 15, 2017 Accepted: November 03, 2017 Published: November 18, 2017 ABSTRACT Avian leukosis virus (ALV) is an oncogenic virus causing a variety of neoplasms in chickens. The group of avian leukosis virus in chickens contains six closely related subgroups, A to E and J. The prevalence of ALVs in hosts may have imposed strong selective pressure toward resistance to ALVs infection. The tvb gene encodes Tvb receptor and determines susceptibility or resistance to the subgroups B, D, and E ALV. In this study, we characterized a novel resistant allele of the tvb receptor gene, tvb r3 , which carries a single-nucleotide substitution (c.298C>T) that constitutes a premature termination codon within the fourth exon and leads to the production of a truncated Tvb R3 receptor protein. As a result, we observed decreased susceptibility to infection by ALV-B, ALV-D and ALV-E both in vitro and in vivo , and decreased the binding affinity of the Tvb R3 receptor for the subgroups B, D, and E ALV envelope glycoproteins. Additionally, we found that the tvb r3 allele was prevalent in Chinese broiler lines. This study demonstrated that premature termination codon in the tvb receptor gene can confer genetic resistance to subgroups B, D, and E ALV in the host, and indicates that tvb r3 could potentially serve as a resistant target against ALV-B, ALV-D and ALV-E infection.
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circular rna alterations are involved in resistance to avian leukosis virus subgroup j induced tumor formation in chickens
Oncotarget, 2017Co-Authors: Xinheng Zhang, Aijun Li, Huanmin Zhang, Xinjian Li, Weiguo Chen, Feng ChenAbstract:// Xinheng Zhang 1, 2, 5, 6 , Yiming Yan 1, 2, 5 , Xiaoya Lei 1, 2, 5 , Aijun Li 3 , Huanmin Zhang 4 , Zhenkai Dai 1, 2, 5 , Xinjian Li 1, 2, 5 , Weiguo Chen 1, 2, 5, 6 , Wencheng Lin 1, 2, 5, 6 , Feng Chen 1, 2, 5, 6 , Jingyun Ma 1, 2, 5, 6 and Qingmei Xie 1, 2, 5, 6 1 College of Animal Science, South China Agricultural University, Guangzhou, 510642, P.R. China 2 Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding, Guangzhou, 510642, P.R. China 3 College of Science and Engineering, Jinan University, Guangzhou, 510632, P.R. China 4 USDA, Agriculture Research Service, Avian Disease and Oncology Laboratory, East Lansing, MI, 48823, USA 5 Key Laboratory of Animal Health Aquaculture and Environmental Control, Guangdong, Guangzhou, 510642, P.R. China 6 South China Collaborative Innovation Center for Poultry Disease Control and Product Safety, Guangzhou, 510642, P.R. China Correspondence to: Qingmei Xie, email: qmx@scau.edu.cn Keywords: circular RNA, ALV-J-resistant chickens, circular-seq, tumor Received: November 25, 2016 Accepted: March 08, 2017 Published: March 22, 2017 ABSTRACT Avian leukosis virus subgroup (ALV-J) is an oncogenic neoplasm-inducing retrovirus that causes significant economic losses in the Poultry industry. Recent studies have demonstrated circular RNAs (circRNAs) are implicated in pathogenic processes; however, no research has indicated circRNAs are involved in resistance to Disease. In this study, over 1800 circRNAs were detected by circRNA sequencing of liver tissues from ALV-J-resistant ( n = 3) and ALV-J-susceptible chickens ( n = 3). 32 differentially expressed circRNAs were selected for analyzing including 12 upregulated in ALV-J-resistant chickens and 20 upregulated in ALV-J-susceptible chickens, besides, the top five microRNAs (miRNAs) for 12 upregulated circRNAs in ALV-J-resistant chickens were analyzed. Gene ontology and KEGG pathway analyses were performed for miRNA target genes, the predicted genes were mainly involved in immune pathways. This study provides the first evidence that circRNA alterations are involved in resistance to ALV-J-induced tumor formation. We propose circRNAs may help to mediate tumor induction and development in chickens.